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Neutrophils are essential components of the innate immune system and they play a critical role in the defense of host against bacterial and fungal infections. The colony stimulating factors are a class of glycoproteins that are required for proliferation, differentiation, and functional activation of hematopoietic progenitor cells. Granulocyte-colony stimulating factor (G-CSF) is a member of this regulatory family of cytokines that specifically stimulates proliferation and maturation of precursor cells in the bone marrow into fully differentiated and functional neutrophils. G-CSF also modulates the biological activities of mature neutrophils in circulation. A bovine G-CSF (bG-CSF) cDNA clone (previously isolated and sequenced in our laboratory) was expressed in Escherichia coli and the biological activities of the solubilized protein from purified inclusion bodies were examined. Flow cytometric analysis of membrane antigen density of neutrophils activated with bG-CSF revealed an upregulation in the expression of CD11a (>114%), CD11b (>148%), CD11c (>87%), and CD18 (>109%). Expression of L-selectin was decreased by more than 43%. There was no change, however, in the expression of CD14. These findings indicate that recombinant bG-CSF (rbG-CSF) expressed in E. coli is biologically active and exerts the same type of effects on neutrophils in vitro as those of human G-CSF (hG-CSF). 相似文献
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Neutrophils play a critical role in defending against bacterial infections. Hematopoietic growth factors are a class of regulatory cytokines that are required for stimulation, proliferation, and differentiation of blood cells. Granulocyte colony stimulating factor (G-CSF) is a cytokine that induces proliferation and maturation of precursor myeloid cells in the bone marrow into fully differentiated neutrophils. G-CSF also modulates the functional activity of mature neutrophils. Treatment with G-CSF significantly enhances neutrophil phagocytic activity and killing of bacteria and fungi. We have isolated and sequenced a cDNA clone encoding bovine G-CSF (bG-CSF) from an endothelial cell cDNA library using primers designed from ovine G-CSF. The full length cDNA is 1460 nucleotides with 585 nucleotides comprising the open reading frame. Sequence analysis shows 95% identity with ovine, 89% with porcine, 85% with human, and 76% with murine G-CSF. The deduced G-CSF protein consists of 174 amino acids with 95% identity to ovine, 86% to porcine, 81% to human, and 71% to murine. The signal peptide of G-CSF is 21 amino acids long which is nine amino acids shorter than that of human and murine G-CSF. RT-PCR analysis shows that neither freshly isolated nor ConA stimulated neutrophils express G-CSF mRNA. Mononuclear cells, however, expressed G-CSF mRNA after 48 h incubation with or without ConA stimulation. 相似文献
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Ali Kadivar Habiballah Rashidzadeh Najmeh Davoodian Hasan Nazari Rohallah Dehghani Tafti Heidar Heidari Khoei Hasan Seidi Samani Jahangir Modaresi Ebrahim Ahmadi 《Reproduction in domestic animals》2021,56(2):287-291
Accurate diagnosis of foetal sex in pregnant mare is helpful for many breeders, both for private or commercial purposes. In this study, in order to pre-natal foetal sexing in equine, we used TaqMan duplex real-time PCR to detect the specific regions of SRY and TSPY genes on extracted cell-free foetal DNA from maternal blood. Peripheral blood samples from 50 pregnant Arabian mares with singleton foetuses were collected. Cell-free foetal DNA was extracted from maternal plasma, and duplex real-time PCR assays were performed with TaqMan probes and primers. Amplification of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene was used as control of DNA extraction procedure. From the 50 sampled mares, 28 cases had female and 22 mares had male foetuses. The final results for 46 samples were conclusive, and from them, 43 cases were predicted correctly. Sensitivity, specificity and accuracy of the test were 90.48%, 96% and 93.48%, respectively. In conclusion, a TaqMan duplex real-time PCR was set up to pre-natal detection of foetal sex in equine. The method was fast and decreased the false-positive and false-negative results. The technique can be used as a routine procedure in farms by collecting only a blood sample. 相似文献
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AbdolMajid Valipour Behrooz Heidari Hamidreza Vaziri S. Mohsen Asghari 《Reproduction in domestic animals》2021,56(10):1349-1357
Kisspeptin, upstream of the hypothalamic-pituitary-gonadal axis, play an essential role in the reproductive process. In the present study, the effect of different types of kisspeptin, including goldfish (Carassius auratus) kiss1 kisspeptin (Kiss1), human kisspeptin (Hkiss) and their combination (Kiss1+H) on the reproductive-related genes (kiss1, Kissr and Cyp19) of adult female goldfish was investigated in comparison with Ovaprim (a synthetic GnRH hormone). Kiss1 and Hkiss were synthesized using a solid-phase synthesis approach. Peptides were injected at a dose of 100 μg/kg body weight. The brain and ovarian tissues of samples were separated for histological studies 24 hr post-injection. The expression of the kiss1, Kissr and Cyp19 genes was measured by RT-PCR. The results showed a significant increase in expression of the reproductive-related genes. Histological analysis revealed higher number of mature oocytes in kisspeptin treated groups compare to other ones. In conclusion, Hkiss and Kiss1+H are the most effective peptides in oocyte maturation and expression of reproductive-related genes. In addition, it seems that kisspeptins in other domestic animals can be used to stimulate the hypothalamus-pituitary-gonadal axis. 相似文献
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Characterization of LORF11, a unique gene common to the three Marek's disease virus serotypes 总被引：1，自引：0，他引：1
The unique open reading frame 11 (LORF11) of Marek's disease virus (MDV) is present in all three serotypes of MDV and is located in the unique long region of the MDV genome. In the serotype 1 Md5 genome, LORF11 comprises 2711 nucleotides and encodes a predicted protein of 903 amino acids. In order to study the biological function of LORF11 we deleted it from the MDV cosmid A6 by using the RecA-assisted restriction endonuclease cleavage method. The recombinant cosmid, A6DeltaLORF11, was transfected into duck embryo fibroblasts (DEF) in conjunction with parental SN5, P89, SN16, and B40 cosmid clones. Recombinant rMd5DeltaLORF11 plaques were evident at 12-13 days after transfection. Polymerase chain reaction amplification of DEF cells infected with rMd5DeltaLORF11 viruses confirmed the deletion of a 2.57-kb fragment resulting in a 296-bp fragment. Three rMd5DeltaLORF11 mutants were generated and their biological functions were studied in vitro and in vivo. In vitro growth characteristics of rMd5DeltaLORF11 viruses were similar to those of parental rMd5, indicating that LORF11 is not essential for replication in vitro. In vivo studies of rMd5DeltaLORF11 mutants showed that they were impaired in viral replication in the lymphoid organs and had 100x lower viremia than chickens infected with the parental rMd5 virus. Furthermore, rMd5-infected chickens horizontally transmitted the virus to contact controls whereas no horizontal transmission occurred in rMd5DeltaLORF11-infected chickens. Three independent deletion mutants were tested and showed the same phenotypes, so it is unlikely that the observed phenotype is because of any random mutation in the genome. Therefore the LORF11 gene of MDV is essential for normal virus replication in chickens and deletion of LORF11 renders an attenuated virus. 相似文献
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Behrooz Hassanpour Mohd Mansor Ismail Zainalabidin Mohamed Nitty Hirawaty Kamarulzaman 《Aquaculture Economics & Management (Blackwell Science)》2013,17(3):218-234
This study investigates the sources of total factor productivity (TFP) growth in rainbow trout production in Iran using data envelopment analysis (DEA). The Malmquist index is then employed to decompose the TFP growth into technical efficiency change and technological progress. We utilized panel data of 207 trout farms in the country over a 5-year period from 2003 to 2007. The results of this study revealed that TFP growth of rainbow trout farming has an increasing trend over the period at an average annual rate of 3.7%. The trend of cumulative technological change is negative and tends to be contrary to cumulative technical efficiency change. Although there is no technological change or innovation on trout farming, the technical efficiency change was found to be the sole source for TFP change, whereas the mean of technical efficiency was estimated to be about 66%. Therefore, there is still a great relative potential for increasing trout production through improvement in managerial efficiency as well as technological progress. The study suggests that Iran also has considerable room to enhance trout aquaculture's TFP growth by shifting the production frontier with adoption of new technologies and improving innovation. 相似文献
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Feuj Roghiyeh Heidari Bahram Dadkhodaie Ali 《Journal of Crop Science and Biotechnology》2018,21(4):309-319
Journal of Crop Science and Biotechnology - Twenty-eight genotypes including triticale, wheat and rye were grown in a greenhouse under control, 14 and 21 dS/m salinity (NaCl and CaCl2, 1:1 ratio)... 相似文献
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M. Ghasemi Varnamkhasti H. Mobli A. Jafari A.R. Keyhani M. Heidari Soltanabadi S. Rafiee K. Kheiralipour 《Journal of Cereal Science》2008,47(3):496-501
Designing the equipment for processing, sorting, sizing and other post-harvesting equipment of agricultural products requires information about their physical properties. In this study, various physical properties of rough rice cultivars were determined at a moisture content of 10% (wet basis). In the case of Sorkheh cultivar, the average thousand grain weight, equivalent diameter, surface area, volume, sphericity, aspect ratio, true density, bulk density and porosity were 21.64 g, 3.35 mm, 31.76 mm2, 20.27 mm3, 39.71%, 0.28, 1269.1 kg/m3, 544.34 kg/m3, and 56.98%, respectively. The corresponding values were 20.52 g, 3.4 mm, 32.58 mm2, 21.06 mm3, 39.88%, 0.29, 1193.38 kg/m3, 471.21 kg/m3, and 60.37% for Sazandegi cultivar. For Sorkheh cultivar, the average static coefficient of friction varied from 0.2899 on glass to 0.4349 on plywood, while for Sazandegi cultivar the corresponding value varied from 0.2186 to 0.4279 on the same surfaces. Angle of repose values for Sorkheh and Sazandegi cultivars were 37.66° and 35.83°, respectively. Linear model for describing the mass of rough rice grain was investigated. Mass was estimated with single variable of kernel length with a determination coefficient as 0.862 for Sorkheh cultivar whereas for Sazandegi cultivar was as 0.860. 相似文献