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91.
92.
An extensive helium plume in the north central Pacific emanates from Loihi Seamount on the flanks of Hawaii. The maximum helium signal is found at a depth of about 1100 meters, the same depth as the near-field plume directly above Loihi Seamount. Although this helium plume is strongest near Hawaii, where the 3He/4He ratio at a depth of about 1100 meters reaches values 28 percent above the atmospheric ratio, it can be detected quite clearly at latitude 24°N, over 400 kilometers to the north. Excess 3He is also present on the same isopycnal between 15°N and 20°N at 135°W, some 2000 kilometers east of the Hawaiian Islands.  相似文献   
93.
In the non‐breeding season, LH release is reduced via dopaminergic systems in the ram. On the other hand, our previous studies demonstrated an opioidergic inhibition of LH release in stallions outside the breeding season. Thus, in the present study we investigated the dopaminergic regulation of LH and prolactin secretion in stallions, considering interactions between dopamine and opioids. To achieve this, stallions (n=8) were treated with the dopamine antagonist sulpiride (0.6 mg/kg), the opioid antagonist naloxone (0.5 mg/kg), sulpiride plus naloxone or saline in December, March and June. Two hours after the respective treatments, they received a GnRH agonist. Sulpiride induced a significant prolactin release which was most pronounced in December, indicating seasonal variations in the inhibition of prolactin secretion by dopaminergic systems. Prolactin concentrations were not changed by naloxone. Neither during nor outside the breeding season, a dopaminergic regulation of LH release could be demonstrated. In contrast, naloxone caused a significant (p < 0.05) LH release, confirming an opioidergic inhibition of LH release. In conclusion, opioidergic regulation of LH and dopaminergic inhibition of prolactin secretion undergo seasonal changes. Neither during nor outside the breeding season, dopaminergic effects on LH release exist in the stallion.  相似文献   
94.
Farming systems research and extension (FSRE), as used by the global Association for Farming Systems Research-Extension, applies to a family of methodologies used to generate, evaluate and disseminate agricultural technologies in association with farmer participation. FSRE shares many attributes with Diagnosis and Design as practiced in agroforestry. The history of FSRE is traced from 1965 to the present, showing the formalization of the methodology and its critical use in sustainable agricultural technology development. In on-farm research, a primary basis for FSRE, research and extension merge in practice. The definition of recommendation domains (a fundamental concept of FSRE) is based on analysis and interpretation of multi-environmental research results as evaluated by varied criteria.In this paper, we present the results of three research projects to demonstrate the nature of farmer criteria for evaluation. Modified Stability Analysis (MSA) is used to demonstrate the relationship of on-farm research to specific extension messages. Design of on-farm research to make it amenable to analysis by MSA is discussed.Florida Agricultural Experiment Station, Journal Series No. R-03113.  相似文献   
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The objectives of this study were to estimate heritability for scrotal circumference (SC) and semen traits and their genetic correlations (rg) with birth weight (BRW). Semen traits were recorded for Line 1 Hereford bulls (n = 841), born in 1963 or from 1967 to 2000, that were selected for use at Fort Keogh (Miles City, MT) or for sale. Semen was collected by electroejaculation when bulls were a mean age of 446 d. Phenotypes were BRW, SC, ejaculate volume, subjective scores for ejaculate color, swirl, sperm concentration and motility, and percentages of sperm classified as normal and live or having abnormal heads, abnormal midpieces, proximal cytoplasmic droplets (primary abnormalities), bent tails, coiled tails, or distal cytoplasmic droplets (secondary abnormalities). Percentages of primary and secondary also were calculated. Data were analyzed using multiple-trait derivative-free REML. Models included fixed effects for contemporary group, age of dam, age of bull, inbreeding of the bull and his dam, and random animal and residual effects. Random maternal and permanent maternal environmental effects were also included in the model for BRW. Estimates of heritability for BRW, SC, semen color, volume, concentration, swirl, motility, and percentages of normal, live, abnormal heads, abnormal midpieces, proximal cytoplasmic droplets, bent tails, coiled tails, distal cytoplasmic droplets, and primary and secondary abnormalities were 0.34, 0.57, 0.15, 0.09, 0.16, 0.21, 0.22, 0.35, 0.22, 0.00 0.16, 0.37, 0.00 0.34 0.00, 0.30, and 0.33, respectively. Estimates of rg for SC with color, volume, concentration, swirl, motility, and percentages of live, normal, and primary and secondary abnormalities were 0.73, 0.20, 0.77, 0.40, 0.34, 0.63, 0.33, -0.36, and -0.45, respectively. Estimates of rg for BRW with SC, color, volume, concentration, swirl, motility, and percentages live, normal, and primary and secondary abnormalities were 0.28, 0.60, 0.08, 0.58, 0.44, 0.21, 0.34, 0.20, -0.02, and -0.16, respectively. If selection pressure was applied to increase SC, all of the phenotypes evaluated would be expected to improve. Predicted correlated responses in semen characteristics per genetic SD of selection applied to SC were 0.87 genetic SD or less. If selection pressure was applied to reduce BRW, the correlated responses would generally be smaller but antagonistic to improving all of the phenotypes evaluated. Predicted correlated responses in SC and semen characteristics per genetic SD of selection applied to BRW were less than 0.35 genetic SD.  相似文献   
98.
The corpus luteum (CL) of the pig lacks luteolytic sensitivity (LS) to prostaglandin (PG) F‐2α until after day 12 of the oestrous cycle, but the mechanisms underlying this phenomenon are poorly understood. As luteolysis involves apoptosis, we hypothesized that critical apoptotic proteins may be deficient in CLs that lack LS. The specific aim of these studies was to examine mRNA expression and protein levels of apoptosis genes/proteins (BAX/Bax, BCLX/Bcl‐x, CASP3/Caspase‐3, CASP8/Caspase‐8, NFΚB1/NFκB, TP53/p53) in porcine CLs collected at different stages of the oestrous cycle. CLs were collected surgically, mRNA and protein extracted, and expression/levels analyzed by semi‐quantitative (SQ) PCR and Western blots, respectively. At the mRNA expression level, only BAX (maximal on day 4) and TP53 (maximal on day 7) showed significant variations during the oestrous cycle. At the protein level, only Bcl‐x and Caspase‐3 showed significant changes during the cycle; Bcl‐x decreased on day 13 and Caspase‐3 increased on day 13. It is concluded that apoptosis‐associated proteins (i.e. Bcl‐x and Caspase 3) may play a critical role in luteolytic sensitivity in the pig.  相似文献   
99.
This study was performed to test the effect that two separate, daily, constant-light regimes of both 9 and 16 h could have on the main parameters of boar-semen quality analysis, as well as on the motile sperm subpopulations structure and the ability of its conservation at 16 degrees C. Results show that both luminous regimes have slight, specific effects on the main parameters of boar-semen quality analysis, as well as on the motile sperm subpopulations structure. Furthermore, the conservation ability at 16 degrees C of boar semen was not significantly different between both photoperiods. When a temporal study was performed, results showed that semen quality and motility parameter changes were stabilized at nearly constant values from the second month of the study to the last month in both luminous regimes, indicating a rapid light-related effect on testicular function. Our results indicate that light regimes oscillating from 9 h daily to 16 h daily are of little importance in the control of boar-semen quality in a farming environment.  相似文献   
100.
The aim of this study was to analyse the effect of filtration through Sephadex on the subpopulation characteristics of the boar semen. For this purpose 3 ml of 16 commercial doses of fresh diluted boar semen were filtered through a Sephadex G‐15/Polypropylene column. Motility parameters were analysed by a CASA system and statistical study was performed by SAS package using the VARCLUS and the FASTLUST procedures. Statistical study revealed four subpopulations in fresh boar semen, as previously had described (Theriogenology 61: 673–690).Total motility was higher in control than in filtered semen, but there were not statistical differences (65.63 ± 9.65 vs 41.40 ± 9.02). Moreover, the analysis did not show many changes neither in the characteristics nor in the distribution of the four subpopulations. As example although ALHmed of filtered samples were slightly higher, there were only significant differences (p < 0.001) in two subpopulations (subpopulation 2 : 2.2 ± 0.05 in control vs 2.7 ± 0.08 in filtered. Subpopulation 3 : 4.5 ± 0.11 in control vs 5.8 ± 0.23 in filtered semen). HME was also statistically different (p < 0.005) in one subpopulation, showing great values in filtered semen (1.7 ± 0.15 vs 3.0 ± 0.30). In conclusion, the filtration by Sephadex/Polypropylene column does not cause strong changes in subpopulation sperm distribution.  相似文献   
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