In vitro comparison of mechanical and degradation properties of equivalent absorbable suture materials from two different manufacturers     

de la Puerta B  Parsons KJ  Draper ER  Moores AL  Moores AP 《Veterinary surgery : VS》2011,40(2):223-227

Objective: To compare the mechanical, knotting, and absorption properties of 3 synthetic absorbable suture materials (polyglactin 910, polydioxanone, poliglecaprone 25) from 2 different manufacturers (Huaiyin Medical Instruments Co. Ltd. and Ethicon Inc.). Sample Population: Suture material samples from the same manufacturing lot for each suture type and manufacturer. Methods: Part 1: 10 samples of each suture were incubated in bovine serum and tested for ultimate load and stiffness at days 0, 1, 7, 14, 21, and 28. Part 2: 10 knotted samples were similarly evaluated, without incubation. Results: Huaiyin polydioxanone had a greater (P<.001) ultimate load on days 0, 1, and 7; and a consistently greater stiffness for the duration of the study, compared with the Ethicon polydioxanone. Ethicon poliglecaprone 25 had a greater ultimate load on days 0, 1, 7 (P<.001); on day 21 Huaiyin had a greater ultimate load. Ethicon poliglecaprone had greater stiffness on days 0, 1, 7 (P<.001). Ethicon polyglactin 910 had a greater (P<.001) ultimate load at all times and a greater stiffness (P<.001) at days 0, 1, 7, and 14 compared with Huaiyin polyglactin 910. Huaiyin polydioxanone and polyglactin 910 had greater knot breaking strengths than Ethicon equivalents (P<.001). Conclusion: Differences in mechanical properties exist between nominally identical suture materials from different manufacturers.  相似文献   

Differentiation of 3T3-L1 fibroblasts to adipocytes induced by transfection of ras oncogenes.   总被引：16，自引：0，他引：16  

M Benito  A Porras  A R Nebreda  E Santos 《Science (New York, N.Y.)》1991,253(5019):565-568

Mammalian 3T3-L1 cells differentiate into adipocytes after continuous exposure to pharmacological doses of insulin or physiological doses of insulin-like growth factor I (IGF-1). Expression of transfected ras oncogenes led to differentiation of these cells into adipocytes in the absence of externally added insulin or IGF-I. Cells transfected with normal ras genes or the tyrosine kinase trk oncogene did not differentiate. Transfection with a dominant inhibitory ras mutant resulted in inhibition of differentiation. Exposure of untransfected 3T3-L1 cells to insulin stimulated formation of the active Ras.GTP complex. These observations indicate that Ras proteins participate in signal transduction pathways initiated by insulin and IGF-I in these cells.  相似文献   

Mevalonate pathway genes expressed in chilli CM334 inoculated with <Emphasis Type="Italic">Phytophthora capsici</Emphasis> and infected by <Emphasis Type="Italic">Nacobbus aberrans</Emphasis> and <Emphasis Type="Italic">Meloidogyne enterolobii</Emphasis>     

Edgar Villar-Luna  Reyna I. Rojas-Martínez  Benito Reyes-Trejo  Olga Gómez-Rodríguez  Emma Zavaleta-Mejía 《European journal of plant pathology / European Foundation for Plant Pathology》2017,148(4):867-881

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The distribution of immune cells in the lungs of classical and atypical ovine pulmonary adenocarcinoma     

Summers C  Benito A  Ortin A  Garcia de Jalon JA  González L  Norval M  Sharp JM  De las Heras M 《Veterinary immunology and immunopathology》2012,146(1):1-7

Ovine pulmonary adenocarcinoma (OPA) is a contagious tumour caused by infection of sheep with Jaagsiekte sheep retrovirus. Two forms of OPA have been identified, classical and atypical, which can be distinguished clinically and pathologically. Most notably classical OPA is progressive until death, while atypical OPA remains subclinical. In the present study the local immune responses in the lungs of cases of atypical OPA were compared with those from classical cases by immunohistochemistry using a panel of mouse anti-sheep mAbs. Distinct differences in the distribution of immune cell subsets in the two forms of OPA were observed. In particular there was an intratumoural influx of T cell subsets and MHC Class II expression on the tumour cells in atypical OPA, neither of which was seen in classical OPA. It is possible that these differences may contribute, at least in part, to determining the progressive course of classical OPA compared with the subclinical nature of atypical OPA.  相似文献   

Pathology in practice. Listeria monocytogenes     

Câmara AC  Lima PM  Batista JS  Feijó FM  Soto-Blanco B 《Journal of the American Veterinary Medical Association》2012,240(3):269-271

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Influence of drip and furrow irrigation systems on nitrogen oxide emissions from a horticultural crop     

Laura Sánchez-Martín  Augusto Arce  Alejandro Benito  Lourdes Garcia-Torres  Antonio Vallejo 《Soil biology & biochemistry》2008,40(7):1698-1706

Irrigation management has an important influence on emissions of nitrous oxide (N₂O) and nitric oxide (NO) from irrigated agricultural soils. In order to develop strategies to reduce the emission of these gases, a field experiment was carried out to compare the influence of different irrigation systems: furrow (FI) and drip-irrigation (DI), on N₂O and NO emissions from a soil during the melon crop season. Two fertilizer treatments were evaluated for each irrigation regime: ammonium sulphate (AS) as a mineral N fertilizer, at a rate of 175 kg N ha^?1; and a control without any N fertilizer (Control). On plots where the AS treatment was applied, drip irrigation reduced total N₂O and NO emissions (by 70% and 33% respectively) with respect to values for furrow irrigation. This was probably due to the lower amount of water applied and the different soil wetting pattern associated with DI. Dry areas of the drip-irrigated plots emitted a similar amount of N₂O to the wet areas (0.45 kg N₂O-N ha^?1) in the Control and greater quantities in the AS treatment (0.92 kg N₂O-N ha^?1 for dry and 0.70 kg N₂O-N ha^?1 for wet areas). We suggest that the N oxide pulses observed throughout the irrigation period on DI plots could have been the result of frequent increases in the soil wetting volume after the addition of water. Denitrification losses (from depths of 0–10 cm) were estimated at 11.44 kg N₂O- N ha^?1 for the AS treatment under FI and at 4.96 kg N₂O-N ha^?1 for DI. Under DI, nitrification was an important source of N₂O, whereas denitrification was the most important source under FI. The addition of NH₄⁺ and the use of DI enhanced the N₂O/N₂ ratio of gases produced through denitrification. The quantity of dissolved organic C (DOC) in the soil generally decreased with addition of NH₄⁺.This work showed that, in comparison with furrow irrigation, drip irrigation is a method that can be used to save water and mitigate emissions of the atmospheric pollutants NO and N₂O.  相似文献   

Evaluation of Osteotomy Accuracy and Rotational and Angular Alignment for Cranial Closing Wedge Ostectomy Performed With and Without Alignment Aids          下载免费PDF全文

Elena S. Addison MA  VetMB  Benito de la Puerta DVM  CertSAS  Diplomate ECVS  Anna Groth Dr. Med Vet. Diplomate ECVDI  Tony Black BVSc  FANZCVSc  Bruce A. Smith BVSc  MS  FANZCVSc  Diplomate ACVS  Neil J. Burton BVSc  CertSAS  DSAS  Michael J. Toscano BSc  MSc  PhD  Adrian M. Wallace BVSc  Diplomate ECVS 《Veterinary surgery : VS》2015,44(1):78-84

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Physical soil degradation induced by deforestation and slope modification in a temperate‐humid environment     

M.E. Varela  E. De Blas  E. Benito 《Land Degradation \u0026amp; Development》2001,12(5):477-484

Between February 1998 and March 1999 physical soil degradation was studied in temperate‐humid NW Spain after deforestation and slope modification. Changes in bulk density, total porosity, pore size distribution, aggregate stability and soil mechanical strength indicated the degradation process. Slope levelling produced the greatest impact. A rapid recovery of physical properties was observed, with the exception of those related to the soil mechanical strength. The increased soil erosion needs agronomic studies to understand the long‐term benefits and adverse aspects of the land‐use changes. Copyright © 2001 John Wiley & Sons, Ltd.  相似文献   

Effects of nursery shading on seedling quality and post-planting performance in two Mediterranean species with contrasting shade tolerance   总被引：1，自引：1，他引：0  

Jaime Puértolas  Luis F. Benito  Juan L. Pe?uelas 《New Forests》2009,38(3):295-308

In Mediterranean climates, seedlings are frequently shaded in the nursery to avoid heat damage and save water. However, the impact of this shading on the seedling quality and transplanting performance of Mediterranean species is not well known. We studied the effect of nursery shading on pre-planting features and post-planting performance of two Mediterranean tree species: the shade-intolerant pioneer Pinus halepensis and the shade-tolerant late-successional Quercus ilex. We grew one-year-old seedlings of both species under 100, 40 and 5% full sunlight. Shade had a low impact on the morphology and physiology of Q. ilex seedlings. In pines, only the deep shade treatment produced low quality seedlings with poor root development. In both species, transference to high light at planting in autumn did not impose any additional stress than that caused by frosts, but initial root growth was impaired in the two shaded treatments in pine. Post-planting growth and survival of oak seedlings showed no difference between treatments. Pine seedlings grown in deep shade showed higher mortality and lower growth after planting than those grown in full sun and intermediate light treatments, while intermediate light only reduced growth. For the nursery culture of Q. ilex seedlings, we advise using low light levels during summer to save water without impairing field performance. In P. halepensis, seedlings should be cultured under full sunlight conditions to maximize post-planting growth, but they can be cultured under intermediate light without impairing survival.  相似文献   

Effect of protease EPg222 obtained from Penicillium chrysogenum isolated from dry-cured ham in pieces of pork loins     

Benito MJ  Rodríguez M  Sosa MJ  Martín A  Córdoba JJ 《Journal of agricultural and food chemistry》2003,51(1):106-111

The fungal protease EPg222 obtained from Penicillium chrysogenum Pg222 isolated from dry-cured ham, was assayed for proteolytic activity in a meat model system based on sterile pieces of pork loins for 32 days. Treated samples showed a significative reduction of total high ionic strength-soluble proteins during the incubation period, as compared with a control incubated without enzyme, both on the surface and in the depth. SDS-PAGE analysis of this protein fraction showed higher hydrolysis of the main myofibrillar proteins H-meromyosin, actin, and tropomyosin in treated samples. Non-protein and amino acidic nitrogen were detected in higher amounts in enzyme-added than in control pieces of loins, both on the surface and in the depth. Thus, addition of enzyme EPg222 to whole pieces of meat results in an increase of protein hydrolysis. The effect of this enzyme could be of great interest for stimulating proteolysis in whole dry-cured meat pieces.  相似文献