Feasibility and repeatability of thermal quantitative sensory testing in normal dogs and dogs with hind limb osteoarthritis-associated pain     

Morika D. Williams  Amy E. Kirkpatrick  Emily Griffith  Javier Benito  Jon Hash  B.D.X. Lascelles 《Veterinary journal (London, England : 1997)》2014,199(1):63-67

The objectives of this study were to determine whether thermal quantitative sensory testing (QST) can be performed in client-owned dogs, is repeatable and whether QST differs between normal dogs and dogs with hind limb osteoarthritis (OA). This clinical, prospective, observational study used clinically normal dogs (n = 23) and dogs with OA-associated hind limb pain (n = 9). Thermal QST was performed in standing dogs using a high-powered light source delivered by a previously validated system. Dogs were tested on two occasions, 2 weeks apart. Five tests were performed on each hind limb at each time point. Repeated measures analysis of variance was used to evaluate the effects of leg, time point and OA/normal status on thermal threshold latencies (TTL). Additionally, paired t tests were used to compare the TTL of left and right limbs within groups and between time points.Thermal thresholds were successfully measured in 32 client-owned dogs without prior training. TTL were significantly different between normal and OA dogs (P = 0.012). There was no difference between limbs (P = 0.744) or time periods (P = 0.572), when analyzed by repeated measures analysis of variance, and no interactions between group and limb, visit and limb, or visit and group. In conclusion, thermal thresholds can be measured in client owned dogs with no prior training and are repeatable from week to week. Further data are required to determine if OA results in thermal hypoalgesia as measured at the distal hind limb and whether this is an indication of central sensitization.  相似文献   

DNA fingerprint of F1 interspecific hybrids from the Triticeae tribe using ISSRs     

A. Carvalho  Manuela Matos  José Lima-Brito  H. Guedes-Pinto  César Benito 《Euphytica》2005,143(1-2):93-99

Inter-simple-sequence repeat (ISSR) were used to fingerprint three kinds of F1 interspecific hybrids: the Portuguese durum wheat cultivar ‘Candial’ × tritordeum, the Portuguese bread wheat cultivar ‘Barbela’ × tritordeum and the Portuguese triticale cultivar ‘Douro’ × tritordeum. Among the 30 primers tested, the number of discriminative primers varies from 13 to 20 in each hybrid combination, the total number of ISSR loci analysed ranged from 101 to 183, and the number of discriminative ISSR loci varied from 50 to 71. ISSR-PCR technique revealed to be a valuable fingerprint system for all F1 hybrid combinations and respective parents analysed here. The complementary use of molecular cytogenetic techniques and molecular markers could allow a more accurately evaluation and characterisation of different plant species.  相似文献   

Component analysis of duration from seeding to heading in rice by the basic vegetative phase and the photoperiod-sensitive phase   总被引：4，自引：0，他引：4  

Te-Tzu Chang  Cheng-Chang Li  Benito S. Vergara 《Euphytica》1969,18(1):79-91

Summary Inheritance of the duration from seeding to heading in rice was analyzed by dividing the vegetative growth period into the basic vegetative phase (b.v.p.) and the photoperiod-sensitive phase (p.s.p.). Concurrent determination of the two physiologic phases on pure lines and hybrids was facilitated by testing vegetative tillers of the same plant under two photoperiods.In nine crosses of photoperiod-sensitive by insensitive arieties where the parents differed appreciably in b.v.p. estimates obtained under a 10-hour photoperiod, two to three Ef genes of relatively discrete effect controlled the F₂ variation in b.v.p. Short b.v.p. was controlled by dominant genes of cumulative but unequal effect. In three other sensitive × insensitive crosses where the parents differed less in b.v.p., the F₂ segregation could be ascribed to two to four pairs of metrical alleles with equal or unequal effect.Concurrent data obtained from sensitive × insensitive crosses grown under a 16-hour photoperiod indicate the action of one or two (duplicate) dominant Se genes in controlling strong sensitivity to a long photoperiod. One insensitive variety appeared to carry a recessive inhibitor, i-Se. The Se gene(s) is epistatic to the Ef genes in the expression of earliness under a short photoperiod. Pooled data also suggest an association between photoperiod sensitivity and a short b.v.p. in a large proportion of F₂ plants.Field data on the two principal components of the vegetative growth period obtained under natural daylength generally indicated agreement with duplicate plantings grown under controlled photoperiods. Segregation for the optimum photoperiod and critical photoperiod under a changing photoperiod in the field plantings probably resulted in modified expression of the Se and Ef genes.Research supported in part by grant GB-2417 from the U.S. National Science Foundation.  相似文献   

Feline Musculoskeletal Pain Index: Responsiveness and Testing of Criterion Validity     

J. Benito  B. Hansen  V. DePuy  G.S. Davidson  A. Thomson  W. Simpson  S. Roe  E. Hardie  B.D.X. Lascelles 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2013,27(3):474-482

Background

Progress in establishing if therapies provide relief to cats with degenerative joint disease (DJD)‐associated pain is hampered by a lack of validated owner‐administered assessment methods.

Hypothesis

That an appropriately developed subjective owner‐completed instrument (Feline Musculoskeletal Pain Index‐FMPI) to assess DJD‐associated impairment would have responsiveness and criterion validity.

Animals

Twenty‐five client‐owned cats with DJD‐associated pain.

Methods

FMPI responsiveness (ability to detect the effect of an analgesic treatment) and validity (correlation with an objective measure) were explored through a stratified, randomized, double blinded, placebo‐controlled, crossover 10‐week clinical study. Meloxicam was administered to effect pain relief. A linear mixed model, backward stepwise regression, and Pearson correlations were used to assess responsiveness and criterion validity with the assumption that the NSAID would increase activity.

Results

Positive responses of cats to placebo (P = .0001) and meloxicam treatment (P = .0004) were detected; however, the instrument did not detect any difference between placebo and meloxicam (linear mixed model), even for the high impairment cases. Percent meloxicam target dose administered, temperament, and total baseline FMPI score were covariates that most affected FMPI scores. Controlling for significant covariates, most positive effects were seen for placebo treatment. Positive treatment effects on activity were detected, but only for the cases designated as most highly impaired.

Conclusions and Clinical Importance

Neither responsiveness nor criterion validity were detected by the inclusion criteria for cases in this study. The data suggest that further work is indicated to understand factors affecting activity in cats to optimize inclusion criteria.  相似文献   

Mars-like soils in the Atacama Desert, Chile, and the dry limit of microbial life     

Navarro-González R  Rainey FA  Molina P  Bagaley DR  Hollen BJ  de la Rosa J  Small AM  Quinn RC  Grunthaner FJ  Cáceres L  Gomez-Silva B  McKay CP 《Science (New York, N.Y.)》2003,302(5647):1018-1021

The Viking missions showed the martian soil to be lifeless and depleted in organic material and indicated the presence of one or more reactive oxidants. Here we report the presence of Mars-like soils in the extreme arid region of the Atacama Desert. Samples from this region had organic species only at trace levels and extremely low levels of culturable bacteria. Two samples from the extreme arid region were tested for DNA and none was recovered. Incubation experiments, patterned after the Viking labeled-release experiment but with separate biological and nonbiological isomers, show active decomposition of organic species in these soils by nonbiological processes.  相似文献   

Efficiency of DNA typing methods for detection of smoked paprika "pimenton de la vera" adulteration used in the elaboration of dry-cured Iberian pork sausages     

Hernández A  Aranda E  Martín A  Benito MJ  Bartolomé T  de Gúa Córdoba M 《Journal of agricultural and food chemistry》2010,58(22):11688-11694

The purpose of this work was to develop a PCR method for the identification of smoked paprika "Pimento?n de la Vera" adulteration with paprika elaborated from varieties of pepper foreign to the la Vera region, in central western Spain. Three autochthonous varieties of pepper, Jaranda, Jariza, and Bola, and the varieties Papri Queen, Papri King, Sonora, PS9794, and Papri Ace, foreign to the La Vera region, were used in the study. Analyses of the ITS and 5.8S rDNA, RAPD-PCR, SSR, and ISSR were tested. RAPD-PCR, SSR, and ISSR analyses allowed differentiation among the varieties of paprika analyzed. There was no difference in the sequence of ITS1-5.8S rDNA-ITS2. In addition, with the RAPD-PCR primers S13 and S22, two molecular markers were obtained of 641 and 704 bp, respectively, which allowed all of the smoked paprika varieties to be differentiated from paprikas elaborated with the five foreign varieties. These two molecular markers were investigated as a basis for detecting the adulteration of smoked paprika with paprika elaborated from foreign varieties of pepper.  相似文献