Conserved eukaryotic fusogens can fuse viral envelopes to cells     

Avinoam O  Fridman K  Valansi C  Abutbul I  Zeev-Ben-Mordehai T  Maurer UE  Sapir A  Danino D  Grünewald K  White JM  Podbilewicz B 《Science (New York, N.Y.)》2011,332(6029):589-592

Caenorhabditis elegans proteins AFF-1 and EFF-1 [C. elegans fusion family (CeFF) proteins] are essential for developmental cell-to-cell fusion and can merge insect cells. To study the structure and function of AFF-1, we constructed vesicular stomatitis virus (VSV) displaying AFF-1 on the viral envelope, substituting the native fusogen VSV glycoprotein. Electron microscopy and tomography revealed that AFF-1 formed distinct supercomplexes resembling pentameric and hexameric "flowers" on pseudoviruses. Viruses carrying AFF-1 infected mammalian cells only when CeFFs were on the target cell surface. Furthermore, we identified fusion family (FF) proteins within and beyond nematodes, and divergent members from the human parasitic nematode Trichinella spiralis and the chordate Branchiostoma floridae could also fuse mammalian cells. Thus, FF proteins are part of an ancient family of cellular fusogens that can promote fusion when expressed on a viral particle.  相似文献   

Complex multicolor tilings and critical phenomena in tetraphilic liquid crystals     

Zeng X  Kieffer R  Glettner B  Nürnberger C  Liu F  Pelz K  Prehm M  Baumeister U  Hahn H  Lang H  Gehring GA  Weber CH  Hobbs JK  Tschierske C  Ungar G 《Science (New York, N.Y.)》2011,331(6022):1302-1306

T-shaped molecules with a rod-like aromatic core and a flexible side chain form liquid crystal honeycombs with aromatic cell walls and a cell interior filled with the side chains. Here, we show how the addition of a second chain, incompatible with the first (X-shaped molecules), can form honeycombs with highly complex tiling patterns, with cells of up to five different compositions ("colors") and polygonal shapes. The complexity is caused by the inability of the side chains to separate cleanly because of geometric frustration. Furthermore, a thermoreversible transition was observed between a multicolor (phase-separated) and a single-color (mixed) honeycomb phase. This is analogous to the Curie transition in simple and frustrated ferro- and antiferromagnets; here spin flips are replaced by 180° reorientations of the molecules.  相似文献   

Transition From Sagebrush Steppe to Annual Grass (Bromus tectorum): Influence on Belowground Carbon and Nitrogen     

Benjamin M. Rau  Dale W. Johnson  Robert R. Blank  Annmarrie. Lucchesi  Todd G. Caldwell  Eugene W. Schupp 《Strength and Conditioning Journal》2011,64(2):139-147

Vegetation changes associated with climate shifts and anthropogenic disturbance have major impacts on biogeochemical cycling. Much of the interior western United States currently is dominated by sagebrush (Artemisia tridentata Nutt.) ecosystems. At low to intermediate elevations, sagebrush ecosystems increasingly are influenced by cheatgrass (Bromus tectorum L.) invasion. Little currently is known about the distribution of belowground organic carbon (OC) on these changing landscapes, how annual grass invasion affects OC pools, or the role that nitrogen (N) plays in carbon (C) retention. As part of a Joint Fire Sciences-funded project called the Sagebrush Treatment Evaluation Project (SageSTEP), we quantified the depth distribution of soil OC and N at seven sites experiencing cheatgrass invasion. We sampled plots that retained sagebrush, but represented a continuum of cheatgrass invasion into the understory. Eighty-four soil cores were taken using a mechanically driven diamond-tipped core drill to a depth of 90 cm, or until bedrock or a restrictive layer was encountered. Samples were taken in 15-cm increments, and soil, rocks, and roots were analyzed for OC and total N. We determined that cheatgrass influences the vertical distribution of OC and N within the soil profile and might result in decreased soil OC content below 60  cm. We also found that OC and total N associated with coarse fragments accounted for at least 10% of belowground pools. This emphasizes the need for researchers to quantify nutrients in deep soil horizons and coarse fragments.  相似文献   

Evaluation of coagulation status in dogs with naturally occurring canine hyperadrenocorticism     

Tyler C. Klose DVM  DACVIM  Kate E. Creevy DVM  MS  DACVIM  Benjamin M. Brainard VMD  DACVA  DACVECC 《Journal of Veterinary Emergency and Critical Care》2011,21(6):625-632

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Anesthesia of Tibetan yak (Bos grunniens) using thiafentanil - xylazine and carfentanil - xylazine     

Cushing A  McClean M  Stanford M  Lohe T  Alcantar BE  Chirife AD 《Journal of zoo and wildlife medicine》2011,42(4):713-717

The use of 0.025 +/- 0.012 mg/kg (median +/- interquartile range) thiafentanil with 0.15 +/- 0.03 mg/kg xylazine (TX) and 0.011 +/- 0.0015 mg/kg carfentanil with 0.25 +/- 0.093 mg/kg xylazine (CX), with dosages based on estimated bodyweight, was used in the anesthesia of 37 Tibetan yak (Bos grunniens) housed within a drive-through animal park setting. The median time to lateral recumbency was 5 and 7 min for each group, respectively. With the addition of propofol in 8 CX animals and 17 TX animals, the anesthetic plane was suitable for a wide range of procedures. The median time to standing recovery following administration of naltrexone was 4 +/- 3.5 min with TX and 7 +/- 1.5 min with CX. There was one fatality and one case of renarcotization in the TX group. Overall, the dosages used in the study provided a reliable and useful anesthetic induction protocol, with TX animals demonstrating a more rapid induction and recovery with less cardiac depression than CX animals.  相似文献   

Tillage effects on near-surface soil hydraulic properties   总被引：1，自引：0，他引：1  

J. G. Benjamin 《Soil & Tillage Research》1993,26(4):277-288

The processes for the formation of porosity are thought to differ between tilled and non-tilled cropping systems. The pores are created primarily by the tillage tool in the tilled systems and by biological processes in non-tilled systems. Because of the different methods of pore formation, the pore size distribution, pore continuity and hydraulic conductivity functions would be expected to differ among tillage systems. The objective of this study was to determine effects of three tillage systems — mold-board plow (MP), chisel plow (CP), and no-till (NT) — on hydraulic properties of soils from eight long-term tillage and rotation experiments. Tillage effects on saturated and unsaturated hydraulic conductivity, pore size distribution, and moisture retention characteristics were more apparent for soils with a continuous corn (CC) rotation than for either a corn-soybean (CS) rotation or a corn-oats-alfalfa (COA) rotation. Pore size distributions were similar among tillage systems for each soil except for three soils with a CC rotation. The MP system increased volume of pores >150 μm radius by 23% to 91% compared with the NT system on two of the soils, but the NT system increased the volume of the same radius pore by 50% on one other soil. The NT system had 30 to 180% greater saturated hydraulic conductivity than either the CP or MP systems. The NT system with a CC rotation showed a greater slope of the log unsaturated hydraulic conductivity; log volumetric water content relationship on two of the soils indicating greater water movement through a few relatively large pores for this system than for either the CP or MP systems.  相似文献   

Semicarbazide formation in azodicarbonamide-treated flour: a model study   总被引：1，自引：0，他引：1  

Becalski A  Lau BP  Lewis D  Seaman SW 《Journal of agricultural and food chemistry》2004,52(18):5730-5734

Semicarbazide was previously found in foods that were in contact with rubber gaskets foamed at high temperatures with a blowing agent azodicarbonamide. Because azodicarbonamide is an approved flour additive in certain countries, we set out to ascertain if semicarbazide is formed during the baking process from flours containing that additive. The levels of semicarbazide in baking flour treated with azodicarbonamide and bread baked from such flours were determined by isotope dilution (13C15N2-semicarbazide) liquid chromatography electrospray tandem mass spectrometry (LC-MS/MS). The samples were homogenized with HCl, extracted with n-pentane, derivatized with 2-nitrobenzaldehyde, and the derivative was extracted with ethyl acetate. After solvent exchange to 10% acetonitrile in water containing 0.1% acetic acid, the samples were analyzed using a 2.1 mm x 150 mm C18 column eluted with 2 mM ammonium formate in water/methanol (40:60). Semicarbazide was formed during the dry heating of commercial azodicarbonamide-containing flours at temperatures of 150-200 degrees C reaching levels of 0.2 mg/kg. Similar levels of semicarbazide were found in the crusts of breads made from azodicarbonamide-treated flour.  相似文献   

Transitional cell carcinomas of the urinary tract in a colony of beagle dogs     

K J Nikula  S A Benjamin  G M Angleton  A C Lee 《Veterinary pathology》1989,26(6):455-461

Gross and light microscopic features of transitional cell carcinomas (TCC) of the urinary tract were examined in Beagle dogs used for the study of the long-term effects of low-dose, whole-body, 60Co gamma radiation. Thirty-eight cases of TCC occurred among 990 dogs that were from 0 to 14 years of age. There was no conclusive evidence of a radiation effect. The 38 TCC were equally divided between male and female dogs, but there was a significant difference in the sex distribution of urethra-origin TCC. Eleven males had a primary urethral TCC compared to only two females. There was no significant difference between the urethra-origin and bladder-origin TCCs in the number of tumors that caused clinical signs, metastasized, or that contributed to the death of the dog. All cases of urethral TCC in male dogs occurred in the prostatic urethra. The majority of these cases were not recognized to be neoplasms at gross necropsy, but microscopic examination revealed the TCC. Our findings differ from previous reports stating that TCC occurs more frequently in female than male dogs, and they especially differ from reports claiming that urethra-origin TCC is predominantly a disease of female dogs.  相似文献   

Adsorption of systemic fungicides and a herbicide by some components of plant tissues,in relation to some physicochemical properties of the pesticides     

Eran Barak  Amos Dinoor  Benjamin Jacoby 《Pest management science》1983,14(3):213-219

Lignin, an important component of plant tissues, adsorbed five systemic fungicides and one herbicide (carbendazim, triadimefon, nuarimol, triarimol, fenarimol and fluometuron) more strongly than bovine serum albumin, cellulose, ethylcellulose or sodium polygalacturonate. Significant correlations were found between the extent of the adsorption of the pesticides on the lignins extracted from three different plant species, and the log Poet (Poet is the octan-1-ol/water partition coefficient) of the compounds. The more lipophilic fungicides triarimol and fenarimol (log Poet about 2.6) were adsorbed to the greatest extent. Fluometuron, triadimefon and nuarimol (log Poet about 2.0) were moderately adsorbed, whereas carbendazim with the lowest log Poet (1.34 at pH 5.0), was adsorbed more than expected from its Poet value. The anomaly of carbendazim is discussed; it is ascribed to it's partial protonation at pH 5.0, whereas the other pesticides were non-ionised.  相似文献   

Primary production of grazed annual natural pasture and of grazed wheat in a semi-arid region of Israel     

R.W. Benjamin  M. Chen  N.G. Seligman  D. Wallach  M.J. al Hadad 《Agricultural Systems》1978,3(3):205-220

The effect of grazing on the above-ground primary production and water use of a fertilised annual pasture and of fertilised sown wheat was studied. Similar studies were made of the effect of cutting on sown wheat. Two simple models—one exponential, the other logistic, both based on the dry weight of the above-ground biomass—were used to describe the primary production in all treatments.The production of undisturbed pasture and undisturbed wheat was 6480 and 7080 kg DM/ha, respectively. Grazing had no significant effect on the total production of the pasture but reduced the DM yield of wheat by up to 32%. Cutting of the wheat reduced the yield by 56%. Water use was similar in all treatments of pasture and wheat and was not directly related to production. The predicted production by both models was similar to observation in the early part of the season but differed from observation in the later part. Possible reasons for the discrepancies are discussed.  相似文献