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41.
Studies were conducted with 66 gilts with synchronised ovulation, all kept in five separate experimental groups (I-V). Each of the animals received 75 mg Dexamethason (DXMS) per die over three consecutive days of pregnancy. Treatment of Groups I through V was started in this order on the 101st, 103rd, 105th, 107th or 109th days of pregnancy. The average intervals that elapsed between the last DXMS injection and onset of parturition were, in the same order, 174, 167, 133, 94 or 74 hours. Births were concentrated on two consecutive days in Group V. DXMS had no significant effect on the average duration and course of parturitions. The lowest average litter and piglet birth weights were recorded from those DXMS groups with shortest pregnancy. No significant mean differences in piglet weight were any longer established between the groups on the tenth day of age. 相似文献
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Partial reference is made to some literature on the formation and properties of gonadotropine from the serum of pregnant mares (PMSG). Also an assessment is made of biological testing methods by which to determine gonadotrophic, follicle-stimulating, and luteinising activities in PMSG. 相似文献
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VL Grillo KE Arzey PM Hansbro AC Hurt S Warner J Bergfeld GW Burgess B Cookson CJ Dickason M Ferenczi T Hollingsworth MDA Hoque RB Jackson M Klaassen PD Kirkland NY Kung S Lisovski MA O'Dea K O'Riley D Roshier LF Skerratt JP Tracey X Wang R Woods L Post 《Australian veterinary journal》2015,93(11):387-393
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Fike KE Wehrman ME Lindsey BR Bergfeld EG Melvin EJ Quintal JA Zanella EL Kojima FN Kinder JE 《Journal of animal science》1999,77(3):715-723
Our hypothesis was that estrus synchronization in beef cattle using melengestrol acetate (MGA) and an injection of progesterone (P4) and 17beta-estradiol (E2) to regress dominant ovarian follicles would improve pregnancy rate (number conceived/number in group) to AI compared with feeding only MGA or injecting PGF2alpha. During 2 yr, peripubertal heifers (n = 52) and cows (n = 327) received either 1) MGA for 18 d (d 0 = 1st d of MGA) plus an injection of P4 and E2 in sesame oil (vehicle) on d 11 to regress persistent ovarian follicles (MGA+P4), 2) MGA for 18 d plus vehicle on d 11 (MGA), or 3) two injections of PGF2alpha 10 d apart (d 7 and 17, PG). Concentration of P4 was assessed in blood samples obtained on d 0, 7, and 17 to indicate estrual status (anestrual or estrual) during treatment to induce estrus synchrony. Observations for detection of estrus occurred every 6 h for 180 h following treatment cessation. Females showing estrus were inseminated 6 to 12 h after estrus detection. Conception to AI was determined by ultrasonography 35 to 40 d later. Conception rate was greater (P < .05) in females in the PG than in those in the MGA group but did not differ from conception rate of females in the MGA+P4 group. Among anestrual females, estrus synchrony rates were greatest (P < .10) among females treated with MGA+P4. Among females that were estrual before treatment cessation, estrus synchrony rates were greater (P < .10) among females treated with MGA+P4 or PG than among those given MGA. Pregnancy rates were greater (P < .05) among females that were anestrual before treatment cessation and treated with MGA or MGA+P4 than among those treated with PG. Estrus synchronization using MGA+P4 and E2 differentially improves estrus synchronization and pregnancy rates among anestrual and estrual beef cattle while maintaining conception rates similar to those of PGF2alpha-treated females. 相似文献
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Treatment was applied, on two farms, to 272 gilts and 169 adult sows, 24 hours after discontinuation of Suisynchron application to the gilts or weaning of piglets of adult sows. Included were 600, 800 or 1,000 IU of PMSG for the gilts and 750, 1,000 oder 1,250 IU of the same batch (Pregmagon-Dessau) for the adult sows for ovarian stimulation. This was followed by application of 500 IU of HCG to the gilts or 300 micrograms Gn-RH vet. "Berlin-Chemie" + 300 IU HCG to the adult sows for synchronised ovulation. Laparotomy was performed on the gilts on the fifth day from PMSG application and on the adult sows on the fourth day. Average ovariotropic stimulation was "mild" in response to lower doses (14.8 follicles in gilts and 20.4 follicles in adult sows) but was "pronounced" in response to medium doses (19.4 or 22.7 follicles, respectively). The pharmacological concept of "mild" exogenic ovarian stimulation by low dosage at the threshold of effectiveness should deserve more attention, last but not least, for breeding aspects. The two groups of animals on both farms differed considerably from one another with regard to reactivity, which cannot be etiologically interpreted for the time being. 相似文献
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Single layer centrifugation of fresh dromedary camel semen improves sperm quality and in vitro fertilization capacity compared with simple sperm washing
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C Malo EG Crichton JM Morrell BS Pukazhenthi JA Skidmore 《Reproduction in domestic animals》2017,52(6):1097-1103
Single layer centrifugation (SLC) through a colloid is a tool for selecting viable mammalian spermatozoa but has not been used previously for fresh dromedary camel sperm. Semen from six camels (2 ejaculates/male) was diluted 1:5 (v:v) or 1:10 (v:v) in a Tris–citrate–fructose buffer for mechanical liquefaction by gentle pipetting. Following liquefaction, semen was processed either by SLC or by centrifugation without a colloid (control). Total and progressive motilities, CASA kinematics, vitality and acrosome integrity (eosin–nigrosin) and plasma membrane integrity (Hypo‐osmotic swelling test; HOST), and fertilizing ability in a heterologous assay (zona‐free goat oocytes) were evaluated. Both total (p = .003) and progressive motilities (p = .003) were higher in SLC‐processed than in control semen samples, irrespective of dilution. Positive HOST values increased when using colloid in 1:5 (p = .001) and 1:10 dilution (p = .010). Colloid‐selected sperm had higher penetration rates than controls (p < .001 and p = .02 for 1:5 and 1:10 dilutions, respectively). However, only the SLC sperm at 1:5 dilution showed higher percentages of pronuclear formation (p = .02) than controls. Dilution effect was only significant for total motility before in vitro fertilization, with higher values for the 1:5 dilution (p = .033). The recovery rates of motile sperm between dilutions were similar (26.1% vs 35.4%; p = .226). In conclusion, SLC is a promising tool for selecting functional dromedary camel sperm and warrants more research. 相似文献
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