Bee communities in forestry production landscapes: interactive effects of local-level management and landscape context     

Miljanic  Andriana S.  Loy  Xingwen  Gruenewald  David L.  Dobbs  Emily K.  Gottlieb  Isabel G. W.  Fletcher  Robert J.  Brosi  Berry J. 《Landscape Ecology》2019,34(5):1015-1032

Landscape Ecology - Land-use change is a key driver of pollinator declines worldwide. Plantation forests are a major land use worldwide and are likely to expand substantially in the near term,...  相似文献   

Conditioned medium of E17 rat brain cells induced differentiation of primary colony of mice blastocyst into neuron-like cells     

Vista Budiariati  Ratih Rinendyaputri  Ariyani Noviantari  Noer Muhammad Dliyaul Haq  Dwi Budiono  Diah Nugrahani Pristihadi  Berry Juliandi  Mokhamad Fahrudin  Arief Boediono 《Journal of veterinary science (Suw?n-si, Korea)》2021,22(6)

BackgroundConditioned medium is the medium obtained from certain cultured cells and contained secretome from the cells. The secretome, which can be in the form of growth factors, cytokines, exosomes, or other proteins secreted by the cells, can induce the differentiation of cells that still have pluripotent or multipotent properties.ObjectivesThis study examined the effects of conditioned medium derived from E17 rat brain cells on cells with pluripotent properties.MethodsThe conditioned medium used in this study originated from E17 rat brain cells. The CM was used to induce the differentiation of primary colonies of mice blastocysts. Primary colonies were stained with alkaline phosphatase to analyze the pluripotency. The morphological changes in the colonies were examined, and the colonies were stained with GFAP and Neu-N markers on days two and seven after adding the conditioned medium.ResultsThe conditioned medium could differentiate the primary colony, beginning with the formation of embryoid-body-like structure; round GFAP positive cells were identified. Finally, neuron-like cells testing positive for Neu-N were observed on the seventh day after adding the conditioned medium.ConclusionsConditioned medium from different species, in this case, E17 rat brain cells, induced and promoted the differentiation of the primary colony from mice blastocysts into neuron-like cells. The addition of CM mediated neurite growth in the differentiation process.  相似文献