小麦根高亲和NO_3~-转运体全长基因(TaNRT2.1)的克隆和鉴定     

印莉萍  黄勤妮  黄海明  米国华  James O Berry 《中国农业科学》2004,37(6):795-800

 在前期获得的 pTaAF 的工作基础上,采用 RACE 方法进一步克隆和鉴定了小麦根 NO3 转运体全长基因-(TaNRT2.1)。将TaNRT2.1和已知的硝态氮转运体基因家族进行同源性比较,指出TaNRT2.1属于HATS中的NRT2家族。Southern　印迹揭示小麦基因组中有1个TaNRT2.1拷贝。Northern　分析示出硝态氮可瞬时诱导TaNRT2.1　mRNA积累,NO 处理 1 h　TaNRT2.1　mRNA很快增加,4　h 达到最大,24　h恢复至诱导前水平,具根专一表达特点。 - 3  相似文献   

Clay plains and geomorphic history of the central Sudan A review     

B.P. Ruxton  L. Berry 《CATENA》1978,5(3-4)

The paper presents an analysis of the Sudan clay plain. Total plain area is 500,000 sq. km., but the paper focuses on an area of 90,000 sq. km.In the east-central Sudan an old erosion surface between600m× 730m above sea level is probably Miocene in age. The main plainlands lie 200 – 300 m below this and date from entertiary times. Evidence of an “African” surface may occur on skeletal volcanoes near Gedaref.Downwarping along NNW-SSE lines formed distinctive basins including the Blue Nile basin. Sediments accumulated in the basins and a sequence of arkose, paludal sandy clay, alluvial sand, silt and clay in the Blue Nile basin is capped by modern silt and clay which has weathered to form a clay soil.The clays on the plain vary in age from upper Pliocene to modern though the weathering seems in balance with a somewhat wetter climate than at present. The clays dominantly on almost level plains are alluvial or lacustrine. The remainder is residual or colluvial weathered material on flat but extremely gently sloping interfluves on higher ground.The clay of the aggradational plains in the Blue Nile basin has been reworked continuously by the rivers and is therefore, much younger than the clay on the degradational plains which is upper Pliocene to Middle Phistocene in age. The present Blue Nile flows in a wide alluvial valley with the channel incised up to 24 m below the general plain.  相似文献   

Production and characterization of a monoclonal antibody against the beta-adrenergic agonist ractopamine   总被引：2，自引：0，他引：2  

Shelver WL  Smith DJ  Berry ES 《Journal of agricultural and food chemistry》2000,48(9):4020-4026

A monoclonal antibody was generated toward the beta-adrenergic agonist ractopamine hydrochloride ?(1R,3R),(1R, 3S)-4-hydroxy-alpha-[[[3-(4-hydroxyphenyl)-1-methylpropyl]amino]methy l]benzenemethanol hydrochloride?. Ractopamine-glutarate-keyhole limpet hemocyanin (KLH) was used as the antigen for antibody generation in mice. Clone 5G10, secreted antibody with isotype IgG1kappa, was used for the development of an immunoassay. The selected antibody was specific for racemic ractopamine with an IC(50) of 2.69 +/- 0.36 ng/mL (n = 15). Antibody binding toward ractopamine was stereoselective with (1R,3R)-ractopamine having an IC(50) of 0.55 +/- 0.09 ng/mL (n = 3). IC(50) values for the (1S, 3R)-, (1S,3S)-, and (1R,3S)-ractopamine stereoisomers were 2.00 +/- 0.37, 140 +/- 23, and 291+/- 32 ng/mL (n = 3), respectively. Phenethanolamine beta-agonists showed low cross-reactivity. Studies using a series of ractopamine metabolites and ractopamine analogues demonstrated structural requirements for the antibody binding. A free phenolic group on the N-butylphenol moiety was required for high-affinity binding because methoxylated analogues and metabolites glucuronidated at this phenol generally had IC(50) values greater than 200 ng/mL. Ractopamine analogues methoxylated or glucuronidated at the ethanolamine phenol had IC(50) values of 0.7-2.6 ng/mL. Lack of a benzylic hydroxyl group was of less importance to antibody binding than was the correct stereochemical orientation (3R) of ractopamine's N-phenylalkyl group. In conclusion, a highly specific monoclonal antibody to ractopamine hydrochloride was developed that could be of potential utility in screening assays.  相似文献   

Soil catenas to estimate ages of movements on normal fault scarps, with an example from the Wasatch fault zone, Utah, USA     

James P. McCalpin  Margaret E. Berry   《CATENA》1996,27(3-4)

Trenches excavated across fault scarps on a 13 ka delta surface at Brigham City, Utah, USA, document four types of soil-catena phenomena. First, the relict summit soil is eroded at the scarp crest, and its components incorporated into the accumulating colluvial wedge at the scarp base. Second, the pre-faulting soil weakens laterally as it is traced scarpward beneath the colluvial wedge from a relict position beyond the scarp toe. Third, soils developed on individual colluvial wedges and on the underlying, pre-faulting surface merge downslope to form a cumulative soil profile at the scarp toeslope. Fourth, soil horizons thicken downslope, probably due to the combined affect of more available moisture, water infiltration, and sediment accumulation.Two soil catenas were used to evaluate the applicability of a continuity approach to paleoearthquake dating, by which percentages of total development time represented by each soil at a fault scarp footslope could be estimated. The continuity approach sets total soil development at the scarp footslope, including any buried pre-faulting soil plus the sum development of all colluvial wedge soils corrected by a slope factor (F), equal to total soil development at the scarp summit. Soil development was characterized by Profile Development Index (PDI) and pedogenic clay parameters, using facies models to estimate parent material values for genetically-different colluvial units, and recognizing inherited soil components in colluvial wedges as parent material properties. The F slope factor, used to correct colluvial wedge soils for development attributable to slope position rather than age, was checked by using a nearby fluvial scarp as an independent control. Our soil data, when compared to independent age estimates, imply that rates of soil development have not been constant over the last 13 ka at Brigham City, but rather were considerably higher than average between 13 ka and 8.3 ka. Higher rates are probably due to a relatively high influx of eolian dust during the late Pleistocene to early Holocene following the lowering of Lake Bonneville, a large paleolake in the western USA. These variable rates of soil development preclude using the continuity approach for paleoearthquake dating at Brigham City. However, promising results showing that generally consistent F factors can be calculated for fault and fluvial scarps leave open the possibility that in areas where soil development rates have been relatively constant over the time period in question, the continuity approach may be a broadly applicable method for paleoearthquake dating.  相似文献   

DORSAL PLANE COMPUTED TOMOGRAPHIC IMAGING OF THE ETHMOID REGION TO EVALUATE CHRONIC NASAL DISEASE IN THE DOG     

Philip D.  Koblik  DVM  MS  Clifford R.  Berry  DVM 《Veterinary radiology & ultrasound》1990,31(2):92-97

Computed tomographic anatomy of the normal canine ethmoid region using both transverse and dorsal imaging plane is described. In the transverse plane, the cribriform plate appears as an irregular heart-shaped to rectangular structure that is generally visualized on no more than one to two contiguous 4-mm slices. In the dorsal plane, the cribriform plate appears as a discrete "V"-shaped structure and can be visualized on four to five contiguous slices. Successful visualization of the cribriform was less dependent on patient positioning for the dorsal plane imaging studies. Computed tomographic findings in four dogs with chronic nasal disease and complete postmortem examination of the ethmoid region indicate that the dorsal imaging plane allows a more accurate assessment of cribriform plate involvement than the transverse imaging plane.  相似文献   

ANSWERS FOR FILM READING SESSION* 1995 AMERICAN COLLEGE OF VETERINARY RADIOLOGY MEETING HONOLULU,HAWAII     

Clifford R. Berry 《Veterinary radiology & ultrasound》1995,36(5):449-451

  相似文献   

Gonadotrophin secretion in ewes with bilateral gonadal hypoplasia     

PJ WRIGHT  DB GALLOWAY  IJ CLARKE 《Australian veterinary journal》1996,73(4):157-158

  相似文献   

Use of anti-inflammatory and analgesic drugs in dogs and cats   总被引：1，自引：0，他引：1  

ADJ WATSON  A. NICHOLSON  DB CHURCH  MRB PEARSON 《Australian veterinary journal》1996,74(3):203-210

SUMMARY: Responses (486) were collated from a survey of 5054 Australian veterinarians on their use of anti-inflammatory and analgesic drugs in dogs and cats. Almost all respondents used glucocorticoids (usually prednisolone) to treat allergic, pruritic dermatoses in dogs, while two-thirds also gave fatty acid supplements and one-half used antihistamines. Almost 60% of respondents initially injected a glucocorticoid (frequently a long-acting preparation) when treating inflammatory skin diseases in dogs. More than 90% of respondents used glucocorticoids to treat immunemediated haemolytic anaemia or thrombocytopenia, and about one-third also gave cytotoxic drugs. Administration of prednisolone on alternate days was generally favoured for long-term enteral steroid therapy. Phenylbutazone was the most preferred treatment for painful or inflammatory musculoskeletal disorders of dogs, but aspirin and pentosan polysulphate were also used widely. Regarding the use of analgesics drugs generally, both narcotic analgesics and non-steroidal anti-inflammatory drugs (NSAIDs) were used more widely in dogs than in cats, but alpha-2 agonists were used similarly in both species. The most commonly used narcotic analgesics were pethidine and buprenorphine in both species, while the NSAIDs used most often were flunixin and dipyrone in dogs and ketoprofen in cats. More than 80% of respondents generally used analgesic drugs with potentially painful surgical procedures, with doses given usually before anaesthetic recovery. Analgesic use rates varied with the condition, ranging from 94% for patients with acute severe trauma, through 60% for cruciate ligament repair and 29% for perineal herniorrhaphy, to about 5% for ovariohysterectomy and dog castration. The three clinical signs most frequently nominated as indicators of pain in dogs and cats were (in descending order) vocalisation, response to handling or palpating the affected area, and mental depression. Other items mentioned frequently were behavioural changes and immobility (in both species), inappetence/anorexia in cats, and altered respiration in dogs.  相似文献   

Host plant stimulation of detoxifying enzymes in a phytophagous insect     

S.J. Yu  R.E. Berry  L.C. Terriere 《Pesticide biochemistry and physiology》1979,12(3):280-284

The midgut microsomal aldrin epoxidase of variegated cutworm larvae (Peridroma saucia, Hübner) fed bean or peppermint leaves was up to 10 and 45 times more active, respectively, than that of larvae fed a basic control diet. Large increases in oxidase activity and cytochrome P-450 levels also occurred in larvae fed mint plant constituents such as menthol menthone, α-pinene, and β-pinene. Mint-fed larvae were more tolerant of the insecticide, carbaryl, than bean-fed larvae.  相似文献   

OSTEOSARCOMA OF THE PATELLA WITH PULMONARY METASTASES IN A DOG     

Michael D.  Lucroy  DVM  MS  Jeffrey N.  Peck  DVM  Clifford R.  Berry  DVM 《Veterinary radiology & ultrasound》2001,42(3):218-220

A 6-year-old neutered male Rottweiler was examined for a progressive right pelvic limb lameness. In radiographs of the right stifle, there was an osteolytic lesion with irregular new bone formation along the cranial aspect of the patella consistent with an aggressive bone lesion. In thoracic radiographs, there were multiple soft tissue nodular opacities throughout the lung fields, consistent with pulmonary metastases. Microscopically, fine needle aspirate samples from the right patella contained pleomorphic spindle cells with cytologic features of osteosarcoma. The presence of pulmonary metastases at the time of initial diagnosis in the dog described herein suggests that osteosarcoma of the patella has the potential for similar aggressive biologic behavior as that seen in dogs with appendicular osteosarcoma.  相似文献