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排序方式: 共有109条查询结果,搜索用时 203 毫秒
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Kuss SK Best GT Etheredge CA Pruijssers AJ Frierson JM Hooper LV Dermody TS Pfeiffer JK 《Science (New York, N.Y.)》2011,334(6053):249-252
Intestinal bacteria aid host health and limit bacterial pathogen colonization. However, the influence of bacteria on enteric viruses is largely unknown. We depleted the intestinal microbiota of mice with antibiotics before inoculation with poliovirus, an enteric virus. Antibiotic-treated mice were less susceptible to poliovirus disease and supported minimal viral replication in the intestine. Exposure to bacteria or their N-acetylglucosamine-containing surface polysaccharides, including lipopolysaccharide and peptidoglycan, enhanced poliovirus infectivity. We found that poliovirus binds lipopolysaccharide, and exposure of poliovirus to bacteria enhanced host cell association and infection. The pathogenesis of reovirus, an unrelated enteric virus, also was more severe in the presence of intestinal microbes. These results suggest that antibiotic-mediated microbiota depletion diminishes enteric virus infection and that enteric viruses exploit intestinal microbes for replication and transmission. 相似文献
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Kiupel M Webster JD Bailey KL Best S DeLay J Detrisac CJ Fitzgerald SD Gamble D Ginn PE Goldschmidt MH Hendrick MJ Howerth EW Janovitz EB Langohr I Lenz SD Lipscomb TP Miller MA Misdorp W Moroff S Mullaney TP Neyens I O'Toole D Ramos-Vara J Scase TJ Schulman FY Sledge D Smedley RC Smith K W Snyder P Southorn E Stedman NL Steficek BA Stromberg PC Valli VE Weisbrode SE Yager J Heller J Miller R 《Veterinary pathology》2011,48(1):147-155
Currently, prognostic and therapeutic determinations for canine cutaneous mast cell tumors (MCTs) are primarily based on histologic grade. However, the use of different grading systems by veterinary pathologists and institutional modifications make the prognostic value of histologic grading highly questionable. To evaluate the consistency of microscopic grading among veterinary pathologists and the prognostic significance of the Patnaik grading system, 95 cutaneous MCTs from 95 dogs were graded in a blinded study by 28 veterinary pathologists from 16 institutions. Concordance among veterinary pathologists was 75% for the diagnosis of grade 3 MCTs and less than 64% for the diagnosis of grade 1 and 2 MCTs. To improve concordance among pathologists and to provide better prognostic significance, a 2-tier histologic grading system was devised. The diagnosis of high-grade MCTs is based on the presence of any one of the following criteria: at least 7 mitotic figures in 10 high-power fields (hpf); at least 3 multinucleated (3 or more nuclei) cells in 10 hpf; at least 3 bizarre nuclei in 10 hpf; karyomegaly (ie, nuclear diameters of at least 10% of neoplastic cells vary by at least two-fold). Fields with the highest mitotic activity or with the highest degree of anisokaryosis were selected to assess the different parameters. According to the novel grading system, high-grade MCTs were significantly associated with shorter time to metastasis or new tumor development, and with shorter survival time. The median survival time was less than 4 months for high-grade MCTs but more than 2 years for low-grade MCTs. 相似文献
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Thompson JJ Yager JA Best SJ Pearl DL Coomber BL Torres RN Kiupel M Foster RA 《Veterinary pathology》2011,48(1):169-181
Molecular assays are widely used to prognosticate canine cutaneous mast cell tumors (MCT). There is limited information about these prognostic assays used on MCT that arise in the subcutis. The aims of this study were to evaluate the utility of KIT immunohistochemical labeling pattern, c-KIT mutational status (presence of internal tandem duplications in exon 11), and proliferation markers--including mitotic index, Ki67, and argyrophilic nucleolar organizing regions (AgNOR)--as independent prognostic markers for local recurrence and/or metastasis in canine subcutaneous MCT. A case-control design was used to analyze 60 subcutaneous MCT from 60 dogs, consisting of 24 dogs with subsequent local recurrence and 12 dogs with metastasis, as compared to dogs matched by breed, age, and sex with subcutaneous MCT that did not experience these events. Mitotic index, Ki67, the combination of Ki67 and AgNOR, and KIT cellular localization pattern were significantly associated with local recurrence and metastasis, thereby demonstrating their prognostic value for subcutaneous MCT. No internal tandem duplication mutations were detected in exon 11 of c-KIT in any tumors. Because c-KIT mutations have been demonstrated in only 20 to 30% of cutaneous MCT and primarily in tumors of higher grade, the number of subcutaneous MCT analyzed in this study may be insufficient to draw conclusions on the role c-KIT mutations in these tumors. 相似文献
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P.B. Best 《African Zoology》2013,48(2):114-123
Since 1979, 245 right whales (excluding calves) have been individually identified in aerial photographs taken annually on the South African coast, using variations in dorsal pigmentation and callosity patterns. White or grey blazes (or both) occurred dorsally in 16,7% of individuals, one form of which (partial albinism) occurred in 3,6% of the calves born but may be sex linked. In 19 animals carrying dorsal marks that were resighted two to seven years later, changes in the appearance of the callosities were noted in 95% of individuals, 58% showing pronounced changes. Definite matches between years were made for 139 cows with calves. The average per capita calving interval, adjusted for incomplete spatial and temporal coverage, was 3,183 ± 0,091 (SD) years, or a calf production rate of 0,314 ± 0,009 (SD) per adult female per year. From a simple model it is shown that rates of population increase as high as that observed (6–7%) can only be maintained with a calf production rate of this order if the age at first parturition is relatively low and/or the adult survival rate high. 相似文献
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Doxsee AL Yager JA Best SJ Foster RA 《The Canadian veterinary journal. La revue veterinaire canadienne》2006,47(8):763-766
Primary neoplasms derived from testicular tissue and in an extratesticular location are extremely rare. Clinical and surgical information was collected and verified from 15 different submitting practices for 12 dogs and 5 cats that spontaneously developed neoplasms of testicular origin after castration. Eleven dogs had Sertoli cell tumors in an extratesticular location. One dog and all 5 cats had an extratesticular interstitial cell tumor. Six animals (1 dog, 5 cats) had developed secondary sexual characteristics that reversed after removal of the tumor. All had a palpable mass in the scrotum or at the site of the original prescrotal incision. No animals died of neoplasia-related disease and no metastases were identified. Several possibilities, including the presence of embryological ectopic tissue or the presence of testicular tissue transplanted during castration, are considered as causal. 相似文献
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经过多年的研究,科学家发现营养物之间会竞争吸收位点,与氨基酸平衡理论相似,各种微量元素之间也存在复杂的互作和拮抗作用。而微量元素的吸收同时与饲料中的协同物质与颉抗物质存在量密切相关。中国饲料中普遍存在的高铜的现象,为了避免高铜带来的氧化应激及可能的亚中毒问题,又不影响其它微量元素铁和锌的吸收,必须添加高铁和高锌,造成了大量无机微量元素的浪费损失,饲料利用率显著下降,同时严重污染环境。在饲料中我们同时添加多种微量元素,只要有一种微量元素过量或不足,微量元素总体利用率就会下降,造成浪费,所以我们强调各种微量元素之间要尽可能平衡地添加,以确保达到最佳利用率。理想微量元素模型是根据大量的试验总结,以较好的试验结果所总结出来的不同种类微量元素之间的比例关系,主要是铁,铜,锰,锌之间的比例及添加量,以确保动物能够以较少而经济的添加量而得到更好的生长,免疫及繁殖效果。本文以铜为例综述了无机微量元素的缺陷及各种有机微量元素的差异,提出了应用理想微量元素模型提高动物生产性能的概念。 相似文献