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北疆苜蓿属植物繁育系统的研究   总被引:3,自引:0,他引:3  
采用等位酶技术,对北疆苜蓿属植物种群的繁育系统进行了研究。结果表明,北疆苜蓿属植物种群为外繁育系统,黄花苜蓿、多变苜蓿、紫花苜蓿基因组成中杂合体过多,均保持较低的自交结实率。参试苜蓿属植物表现某种程度的内繁育衰退现象,地理或生殖隔离等因素可能是导致个别种群出现内繁育衰退现象的原因之一。对北疆苜蓿属遗传资源保护时,既要选择遗传基础丰富的种和种群以保持最多的遗传变异,还要注意选择具有不同等位的种群以保持原有的基因和基因频率。  相似文献   
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A total of 295 birds belonging to 19 species of 7 families of wild Passeriformes were examined by haemagglutination-inhibition test. The birds were caught for an international research program "Balt" at the time of autumn migration (August-September 1984). Their blood sera were examined for antibodies against 6 arbovirus antigens of the genera Alphavirus (Sindbis-SIN) and Flavivirus (tick-borne encephalitis-TBE, West Nile-WN) and family Bunyaviridae (Tahyna-TAH, Calovo-CVO and Bhanja-BHA). Antibodies against all studied viruses were detected at different frequencies: SIN 6.4%, TBE 7.1%, WN 9.7%, TAH 16.3%, CVO 12.1%, and BHA 1.0%.  相似文献   
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Four infectious agents were isolated from the ticks Ixodes ricinus (L.) collected in the recreational area and park-forest of Prague. On the basis of cultivation, staining, biochemical, serologic properties, pathogenicity for animals and histological tests they were identified as Francisella tularensis with the following features: they are short, gram-negative rods of approximate dimensions of 0.3 X 0.8 micron, growing in enriched media after 3-4 day incubation at 37 degrees C. They form small circular, at first transparent, later greyish turbid colonies with regular rims. They are little active biochemically. They are susceptible to streptomycin and some broad spectrum antibiotics. They react positively with tularemic serum, but in lower titres than those in which this serum reacts with standard antigen. The microbes are highly pathogenic for mice, guinea-pigs, young rats, in which a massive bacteriemia occurs before death, but they do not kill rabbits. They multiply well in chick embryo, but do not grow in cell or tissue cultures. The most important histologic changes were observed in liver and spleen of mice. No pathologic changes were found in brain, lungs, heart, kidneys. Necroses were found in liver and in their marginal zones the microbes were present. Conspicuous were changes in numerous hepatocytes which became enlarged due to microbial multiplication and finally transformed into "sacs" packed with microbes. Histological and electronoptical examination showed that these are intracellular parasites fringed with a light lytic zone. Discussed is the problem to what extent the properties of the isolated strains are typical of F. tularensis as well as the importance of their detection from the aspect of epidemiology and differential diagnostics.  相似文献   
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A damaging virus isolated in the Netherlands from lettuce was studied and compared with a virus isolated from dandelion orginating from Czechoslovakia. It was found to biologically resemble dandelion yellow mosaic virus incompletely described from dandelion and lettuce in Great Britain (Kassanis, 1944, 1947) and from dandelion in Germany (Hein, 1963). Mechanical transmission was greatly improved by buffer solution and transmission byMyzus persicae seemed to be in the non-persistent manner. Longevity in vitro of the virus hardly exceeded one day. Thermal inactivation was between 60 and 65 °C and the dilution end-point was between 10 000 and 100 000. It was still infectious in leaf material dried and stored over CaCl2 at 4 °C for 6 1/2 years. The virus was isolated and purified with difficulty and was found to consist of one type of spherical particle of ca 30 nm diameter, with a sedimentation coefficient of 159 S, a buoyant density of 1.42 g.cm?3 and an A260/A280 ratio of 1.67. An antiserum was prepared with a titre of 256 in the agar double-diffusion test. The virus could be identified in crude extracts from lettuce andChenopodium amaranticolor by enzyme-linked immunosorbent assay (ELISA), but not by agar double diffusion. It could only be visualized in crude sap in the electron microscope after trapping of virus particles on antiserum-coated grids. The virus cannot yet be assigned to any known virus group. It is of potential economic importance to lettuce because of its occurrence in widely differing regions in Europe, its aggressiveness and virulence on 22 out of 23 lettuce cultivars tested (and on endive) and its pathogenicity toLactuca genotypes which are resistant to lettuce mosaic virus and other important pathogens of lettuce. ‘Laibacher Eis’ was the only cultivar showing some tolerance.  相似文献   
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A comparison was made between the genes in 29 new selections of wild emmer wheat resistant to yellow rust over wide geographic areas and the previously extensively studied selectionTriticum dicoccoides G-25. In 23 selections the resistance may be conferred by 1 dominant gene; these include 11 selections in which the gene is different from the dominant gene in sel. G-25 and two others in which the genes were closely linked or allelic to the gene in G-25, differing from sel. G-25 by race-specificity. Two dominant genes different from the gene in sel. G-25, seem to be present in one selection. In five selections the resistance may be conferred by one or two recessive genes, including three instances in which the recessive gene was associated with a dominat gene. Our findings show that at least 19 out of the 29 selections studied possess genes which are different from the gene inT. dicoccoides sel. G-25.Samenvatting In dit onderzoek werden 29 nieuwe resistente wilde-emmer selecties (Triticum dicoccoides) gekruist met de reeds uitvoerig bestudeerde resistente selectie G-25, om na te gaan of de resistentie van de nieuwe selecties wordt veroorzaakt door genen op dezelfde locus als het dominante gen in sel. G-25 of dat er andere loci bij zijn betrokken. De ouders, de F1-en F2-populaties van een bepaalade selectie werden in het kiemplantstadium getoetst met één Israëlisch gele-roest isolaat van fysio 2E0 of van fysio 2E18. In de uitsplitsende F2-populaties werden de niet-sporulerende planten als resistent beschouwd en de sporulerende als vatbaar.In de F2-populaties van 12 herkomsten werden geen vatbare planten gevonden, hetgeen er op duidt dat de resistentie wordt veroorzaakt door een gen op dezelfde locus als het gen in G-25 of door een gen dat neuw gekoppeld is aan het gen in G-25. Voor twee van deze herkomsten kan op basis van een fysio-specifieke interactie worden vastgesteld dat de resistentie berust op allelen die verschillen van het allel in sel. G-25. In 11 herkomsten werd een uitsplitsing voor twee dominante gene gevonden (RS=151), waarbij het tweede dominante gen uit de getoetste nieuwe selectie afkomstig is. De aanwezigheid van twee dominante genen verschillend van het gen in sel. G-25 werd gevonden in één herkomst (631). In de overige vijf selecties bleek de resistentie te worden veroorzaakt door één of twee recessieve genen waarnaast in drie gevallen ook nog een dominant gen werd gevonden.De resultaten tonen aan dat tenminste 19 van de 29 bestudeerde selecties resistentiegenen bezitten die verschillen van het gen inT. dicoccoides sel. G-25. Slechts in twee van deze selecties kan het gen allel zijn met het gen in sel. G-25.  相似文献   
30.
1. An experiment was conducted to determine the effect of different dietary protein contents on the performance of naked neck (Na/na) and normally feathered (na/na) broilers.

2. Chicks from the two genotypes were reared in wire‐floored cages and divided at random into 3 groups. Birds were fed on high protein (HP, 12.99 MJ ME, 238 g crude protein/kg and 12.94 MJ ME, 216 g crude protein/kg from 0 to 3 and 3 to 7 weeks, respectively), medium protein (MP, 12.99 MJ ME, 219 g crude protein/kg and 12.87 MJ ME, 201 g crude protein/kg from 0 to 3 and 3 to 7 weeks), and low protein (LP, 12.94 MJ ME, 205 g crude protein/kg and 12.75 MJ ME, 184 g protein/kg from 0 to 3 and 3 to 7 weeks) diets.

3. The LP diets resulted in a significantly lower daily body weight gain of males from 0 to 3 weeks. Dietary protein content had no effect on body weight gain from 3 to 7 weeks, body weight at 7 weeks, and the food intake of birds. Carcase composition of birds from both genotypes was unaffected by dietary protein.

4. Naked neck birds had significandy higher body weights at 7 weeks. Yields of carcase and breast of Na/na males were significantly higher than those of na/na males. There were no significant differences between females from the two genotypes as regards carcase yield.

5. It was concluded that the dietary protein requirements of naked neck birds were similar to those for normally feathered birds.  相似文献   

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