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91.
A 4.75-year old Simmental cow was presented with symptoms of colic and ileus. The clinical signs and blood analysis resulted in the diagnosis of suspected primary hypoadrenocorticism (Addison’s disease). Although Addison’s disease has been frequently described in other domestic mammals, to our knowledge, this disease has not previously been reported in cattle.  相似文献   
92.
The National Environmental Policy Act (NEPA) of 1970 changed the landscape of natural resource management by requiring federal agencies to assess the environmental consequences of their proposed actions and to include the public in their decision-making processes. Of all federal agencies in the United States, the Forest Service prepares the most Environmental Impact Statements under NEPA. The U.S. Forest Service manages the National Forest System, public forestlands comprising approximately 9% of the United States land area. The overall objectives of this study were to (1) determine the litigants, success rates, and management activities disputed for NEPA litigation involving the Forest Service from 1970 to 2001 and (2) examine differences and patterns in cases among the U.S. District, Circuit, and Supreme Courts. Methods include a historical analysis of published court cases and results show an increasing trend in the number of NEPA-Forest Service cases in the federal courts. Environmental groups were the most common litigants in NEPA-Forest Service cases and timber harvesting, management plans, and endangered species were the subject of the majority of cases in both the U.S. District Court and the U.S. Circuit Court of Appeals. The Forest Service won a preponderance of cases in which they were involved with success rates of 60% in U.S. District Court, 57% in the U.S. Circuit Court of Appeals, and 100% in the U.S. Supreme Court.  相似文献   
93.
Neurologic diseases are common in domestic cats, and infectious agents are suspected to be the primary cause in 30–45% of cases. Among infectious etiologies, those of bacterial origin are only sporadically characterized in the literature, with few of these reports correlating gross and histologic findings with confirmatory bacteriologic identification. Here, we describe bacterial meningitis and meningoencephalomyelitis associated with Pasteurella multocida in 3 domestic cats. Purulent exudate expanding the cerebral meninges was grossly evident in 2 of the cases. In all 3 cases, histologic changes included multifocal suppurative-to-necrosuppurative meningitis and/or meningoencephalomyelitis of variable severity. Intralesional colonies of gram-negative, short rod-shaped to coccobacillary bacteria were evident histologically in only 1 case. P. multocida was confirmed by routine bacteriologic culture in all cases. Based on our cases, we hypothesize that the upper respiratory system serves as the main portal of entry for P. multocida, leading to invasion of the central nervous system and possible systemic hematogenous dissemination. A case series of meningoencephalomyelitis associated with P. multocida infection in cats has not been reported previously, to our knowledge. We also review briefly other causes of meningoencephalomyelitis in cats.  相似文献   
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This study aimed to evaluate the effect of coffee drinking on clinical markers of diabetes and metabolic syndrome in Zucker rats. Diabetic Zucker rats with metabolic syndrome and control Zucker rats were used for in vivo tests. The animals received daily doses of coffee drink by gavage for 30 days. After the treatment, the levels of glucose, triglycerides, total cholesterol and fractions, creatinine, uric acid, activity of aspartate aminotransferase and alanine aminotransferase were evaluated. Urea and creatinine levels were also analyzed in urine. By collaborating in the modulation of the metabolic syndrome and diabetes mellitus type 2, coffee drink helped in reducing serum glucose, total cholesterol and triglycerides. The results demonstrate that treatment with roasted coffee drink, because of its hypoglycemic and hypolipidemic effect, is efficient in the protection of animals with metabolic syndrome and diabetes mellitus type 2.  相似文献   
96.
During thermal milk processing, severe oxidation can occur, which alters the technological and physiological properties of the milk proteins. Due to differences in composition and physicochemical properties, it can be expected that the particular milk proteins are differently affected by oxidative damage. Therefore, the protein-specific distribution of oxidation products in the heated milk proteome was investigated. Raw and heated milk samples were separated by one-dimensional gel electrophoresis. Protein oxidation was visualized by Western blot after derivatization of protein carbonyls with 2,4-dinitrophenylhydrazine. Thus, α-lactalbumin displayed enhanced oxidation compared to β-lactoglobulin, despite its lower concentration in milk. Highly selective oxidation was detected for a previously unassigned minor milk protein. The protein was identified by its peptide mass fingerprint as a variant of α(S1)-casein (α(S1)-casein*). Similar oxidation patterns were observed in several commercial milk products.  相似文献   
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  1. The Balbina hydropower dam in the Central Amazon basin, established in the Uatumã River in the 1980s, is emblematic for its socio-environmental disaster. Its environmental impacts go far beyond the reservoir and dam, however, affecting the floodplain forests (igapó) in the downstream area (dam shadow), which have been assessed using a transdisciplinary research approach, synthesized in this review.
  2. Floodplain tree species are adapted to a regular and predictable flood pulse, with high- and low-water periods occurring during the year. This was severely affected by the operation of the Balbina dam, which caused the suppression of both the aquatic phase at higher floodplain elevations and the terrestrial phase at lower floodplain elevations (termed the ‘sandwich effect’).
  3. During the period of construction and reservoir fill, large-scale mortality already occurred in the floodplains of the dam shadow as a result of reduced stream flow, in synergy with severe drought conditions induced by El Niño events, causing hydraulic failure and making floodplains vulnerable to wildfires.
  4. During the operational period of the dam, permanent flooding conditions at low topographical elevations resulted in massive tree mortality. So far, 12% of the igapó forests have died along a downstream river stretch of more than 125 km. As a result of flood suppression at the highest elevations, an encroachment of secondary tree species from upland (terra firme) forests occurred.
  5. More than 35 years after the implementation of the Balbina dam, the downstream impacts caused massive losses of macrohabitats, ecosystem services, and diversity of flood-adapted tree species, probably cascading down to the entire food web, which must be considered in conservation management.
  6. These findings are discussed critically, emphasizing the urgent need for the Brazilian environmental regulatory agencies to incorporate downstream impacts in the environmental assessments of several dam projects planned for the Amazon region.
  相似文献   
100.
The aim of this study was to evaluate the treatment of bovine semen with the pan-caspase inhibitor benzyloxycarbonyl-Val-Ala-Asp-fluoromethyl ketone (Z-VAD-FMK), before or after freezing on semen quality. After the initial assessment, sperm from 4 bulls were pooled (Experiment 1) and cryopreserved in BioXcell containing 0, 20 and 100 μM Z-VAD-FMK. After thawing semen viability, motility, membrane integrity, as well as DNA fragmentation and ΔΨm were evaluated. In Experiment 2, bovine frozen/thawed sperm were incubated for 1 hr with 0, 20 and 100 µM Z-VAD-FMK before assessing the semen quality. The treatment with Z -VAD-FMK before cryopreservation improved post-thawing sperm motility compared to the control group (p < .05), while no differences were recorded in sperm viability and membrane integrity among groups (on average 86.8 ± 1.5 and 69.1 ± 1.4, respectively). Interestingly, at the highest concentration, DNA fragmentation decreased (p < .05), while the percentage of spermatozoa with high ΔΨm increased (p < .05). The results of Experiment 2 showed that 1-hr treatment with Z-VAD-FMK did not affect sperm motility and viability (on average 63.4 ± 5.8 and 83.7.1 ± 1.2, respectively). However, Z-VAD-FMK improved sperm membrane integrity (p < .05) and at the highest concentration tested decreased the proportion of sperm showing DNA fragmentation (p < .05). No differences were recorded in the percentage of spermatozoa with high ΔΨm (on average 57.0 ± 11.4). In conclusion, the treatment with 100 µM of the caspase inhibitor Z-VAD-FMK before freezing increased bovine sperm mass motility and ΔΨm, while decreasing sperm DNA fragmentation. Treatment of semen after thawing with 100 µM Z-VAD-FMK improved sperm membrane integrity and reduced DNA fragmentation.  相似文献   
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