Experimental clinical and pathomorphological studies on young cocks after feeding mercury-disinfected seed with and without selenium supplementation     

O Schulz  F Berschneider  I Schulz  G Wilsdorf  B Westphal 《Archiv fuer experimentelle veterinaermedizin》1988,42(2):274-281

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Use of enzyme immunoassay and reverse-phase high-performance liquid chromatography to detect and confirm identity of dexamethasone in equine blood.     

A Friedrich  R Schulz  H H Meyer 《American journal of veterinary research》1992,53(12):2213-2220

An enzyme immunoassay (EIA) was developed for detection of dexamethasone in equine blood. Dexamethasone 21-hemisuccinate-bovine serum albumin was used for immunization of rabbits, and prednisolone 21-hemisuccinate-horseradish peroxidase was used as enzyme conjugate. The assay had sensitivity in the low-picogram range (detection limit, 0.3 pg/well, 50% inhibition of binding at 4.5 +/- 0.7 pg/well). Apart from cortisol, which was recognized by the antiserum at concentration > 8.5 ng/ml, the dexamethasone antiserum failed to interfere with endogenous steroids, but cross-reacted with triamcinolone, flumethasone, and betamethasone. Thus, the antiserum was used to perform simultaneous screening for these synthetic glucocorticoids and to confirm their identity by combining reverse-phase high-performance liquid chromatography (RP-HPLC) and EIA. The immunoreactivity obtained by direct serum measurements was characterized by means of 2 independent RP-HPLC systems. Serum extracts were submitted to RP-HPLC systems I and II, and the fractions were tested by EIA. Immunoreactive peaks were identified by comparing their retention time with that of the standard glucocorticoids used for calibration. Coinjection of an internal standard (methylprednisolone) in RP-HPLC system II yielded reproducible relative retention times. The effectiveness of the test system was evaluated, using blood from a horse treated with commonly used veterinary preparations of dexamethasone. Administration of the free alcohol of dexamethasone and of dexamethasone 21-trioxaundecanoate, both given IV, was detected, and the identity of each was confirmed for up to 48 hours. Intramuscular administration of dexamethasone 21-isonicotinate was continued for at least 14 days after injection of a therapeutic dose.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Osseous hydatidosis in a patas monkey   总被引：1，自引：0，他引：1  

M E Fowler  S Silverman  T A Schulz  N Baker 《Journal of the American Veterinary Medical Association》1974,165(9):840-844

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Technology of aerogenic immunization against swine erysipelas under practice conditions     

H M?hlmann  M Meese  P St?hr  V Schulz 《Monatshefte für Veterin?rmedizin》1970,25(21):829-832

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Microangiopathies in septicemic erysipelas characterised by disturbance of blood coagulation     

L C Schulz  K H B?hm  F Kl?pper 《DTW. Deutsche tier?rztliche Wochenschrift》1971,78(21):563-569

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Methionine supplementation of practical broiler rations     

S. Bornstein  Bianka Lipstein 《British poultry science》1964,5(2):167-174

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Studies on cultivation of parasitic larval forms of Hemonchus contortus in vitro     

H P Schulz 《Berliner und Münchener tier?rztliche Wochenschrift》1967,80(5):89-96

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Chlamydiosis in captive raptors     

M E Fowler  T Schulz  A Ardans  B Reynolds  D Behymer 《Avian diseases》1990,34(3):657-662

Chlamydia psittaci was isolated from four red-tailed hawks (Buteo jamaicensis) that died suddenly and from seven birds that survived at a raptor rehabilitation center in California in 1983. One hundred captive raptors representing 14 species in five families were subsequently tested serologically and by direct cloacal culture. C. psittaci was isolated from seven clinically normal birds. Forty-four percent of the raptors were considered positive using an enzyme-linked immunosorbent assay (ELISA), and 19% were suspects. The ELISA was repeated on 54 raptors in 1986. Forty-one percent of the birds were considered positive, and 35% were suspect, indicating that C. psittaci is endemic in the population.  相似文献   

Experimental udder infection with Escherichia coli. 3. Morphological changes directly to subepithelially situated structures in the area of the glands of the milk cistern and the large milk ducts     

P Sterba  G Michel  J Schulz 《Archiv fuer experimentelle veterinaermedizin》1990,44(5):757-764

Alterations were recorded from capillaries and connective tissue by means of optical light and electron microscopy in investigations of subepithelial structures, following experimental Escherichia coli infection. These alterations led to an impairment of the blood-udder barrier and thus to increased extravasation of cells (especially neutrophilic granulocytes). They were found to accumulate in subepithelial connective tissue and, eventually, to penetrate in high quantity the epithelium proper, and, consequently, to be considerably involved in alterations to the latter.  相似文献   

Cases of poisoning in the district of Neubrandenburg and inferences for their prevention     

O Schulz  K Kirchner 《Monatshefte für Veterin?rmedizin》1972,27(22):872-875

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