Epithiospecifier protein from broccoli (Brassica oleracea L. ssp. italica) inhibits formation of the anticancer agent sulforaphane     

Matusheski NV  Swarup R  Juvik JA  Mithen R  Bennett M  Jeffery EH 《Journal of agricultural and food chemistry》2006,54(6):2069-2076

In some cruciferous plants, epithiospecifier protein (ESP) directs myrosinase (EC 3.2.3.1)-catalyzed hydrolysis of alkenyl glucosinolates toward epithionitrile formation. Here, for the first time, we show that ESP activity is negatively correlated with the extent of formation of the health-promoting phytochemical sulforaphane in broccoli (Brassica oleracea L. ssp. italica). A 43 kDa protein with ESP activity and sequence homology to the ESP of Arabidopsis thaliana was cloned from the broccoli cv. Packman and expressed in Escherichia coli. In a model system, the recombinant protein not only directed myrosinase-dependent metabolism of the alkenyl glucosinolate epi-progoitrin [(2S)-2-hydroxy-3-butenyl glucosinolate] toward formation of an epithionitrile but also directed myrosinase-dependent hydrolysis of the glucosinolate glucoraphanin [4-(methylsulfinyl)butyl glucosinolate] to form sulforaphane nitrile, in place of the isothiocyanate sulforaphane. The importance of this finding is that, whereas sulforaphane has been shown to have anticarcinogenic properties, sulforaphane nitrile has not. Genetic manipulation designed to attenuate or eliminate expression of ESP in broccoli could increase the fractional conversion of glucoraphanin to sulforaphane, enhancing potential health benefits.  相似文献   

Characterization and pathogenicity studies of Vibrio parahaemolyticus isolated from diseased freshwater prawn, Macrobrachium rosenbergii (de Man)     

Chinmayee Priyadarshini Khuntia  Basant Kumar Das  Biswa Ranjan Samantaray  Surya Kanta Samal  & Bibhutendu Kumar Mishra 《Aquaculture Research》2008,39(3):301-310

Vibrio parahaemolyticus, an important pathogen of freshwater prawn, Macrobrachium rosenbergii causes high mortality. Four strains of V. parahaemolyticus were isolated from the gill, haepatopancreas, hemolymph and carapace of diseased prawn. The species were characterized based on biochemical and serological tests. On performing an antibiotic assay, these strains were found to be highly susceptible to cephalaxin, tetracycline and erythomycin. During the in vitro pathogenicity test, all the strains were found to be positive to the Congo red binding assay and to be haemolytic in nature, whereas an in vivo pathogenicity test revealed that 2 × 10⁹ CFUs mL^?1of bacteria induced disease symptoms such as black colouration on the carapace, red discolouration of the exoskeleton and loss of appendages within 6 days and 80% mortality. In histopathological studies, a prominent necrosis was seen in gill lamellae, and branchial arches were thickened at places due to hyperplasia and haemocytic infiltration. Hepatopancreatic tissue showed dilation of tubules, vacuolation of hepatocytes and marked necrosis in acinar cells. Development of an immunological technique for the detection and screening of V. parahaemolyticus infection and its treatment is highly important in freshwater prawn aquaculture.  相似文献   

Dietary β‐1,3‐glucan improves the immunity and disease resistance of freshwater prawn Macrobrachium rosenbergii challenged with Aeromonas hydrophila     

Pramoda Kumar Sahoo  Abhilipsa Das  Swagatika Mohanty  Bikash Ranjan Mohanty  Bindu R Pillai  Jyotirmaya Mohanty 《Aquaculture Research》2008,39(14):1574-1578

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Efficacy of moxidectin against naturally acquired nematode infections in cattle.     

S Ranjan  C Trudeau  R K Prichard  R von Kutzleben  D Carrier 《Veterinary parasitology》1992,41(3-4):227-231

The efficacy of moxidectin, a new endectocide against natural nematode infections, was evaluated. Twenty-five calves were divided into two groups of eight calves each and a third group of nine calves. Moxidectin was administered s.c. to two groups (I, 0.2 mg kg-1 body weight (BW); II, 0.3 mg kg-1 BW) and the third group (III) received the vehicle (placebo) s.c. Two pre-treatment and one post-treatment faecal nematode egg count determinations were made from all calves, and they were necropsied 2 weeks after treatment for the identification and enumeration of nematode parasites. Group III calves, which received the drug-free vehicle, harboured eight species of nematodes (Ostertagia ostertagi, Trichostrongylus axei, Cooperia oncophora, Cooperia punctata, Nematodirus helvetianus, Trichuris discolor, Oesophagostomum radiatum and Dictyocaulus viviparus). The mean total worm burden for this group was 8935. There was a significant reduction in the numbers of many species of nematodes (Ostertagia, Trichostrongylus, Cooperia and Nematodirus) in both treated groups. Cooperia oncophora was reduced by 94% in Group I and by 96% in Group II, while all other nematode species were reduced by 99%. Immature stages of Ostertagia and Nematodirus were significantly reduced in the two treated groups. Two weeks after treatment, the mean faecal egg counts of both treated groups were reduced by more than 98%. There was no significant difference in mean total worm burdens or egg counts between the two treated groups.  相似文献   

Distribution analysis of single nucleotide polymorphisms related to feet and legs and their association with lameness in Vrindavani cattle     

Prakash  Chandra  Gaur  Gyanendra Kumar  D R  Pruthviraj  Sahoo  Nihar Ranjan 《Tropical animal health and production》2020,52(2):851-858

Tropical Animal Health and Production - Lameness is one of the most serious economic problems in dairy animals. The present study was undertaken to identify the SNPs associated with lameness in...  相似文献   

Selection for resistance to macrocyclic lactones by Haemonchus contortus in sheep.     

S Ranjan  G T Wang  C Hirschlein  K L Simkins 《Veterinary parasitology》2002,103(1-2):109-117

An anthelmintic-sensitive Haemonchus contortus strain was selected for moxidectin and ivermectin resistance concurrently for 22 generations. Treatment with 0.002 mg moxidectin/kg BW or 0.02 mg ivermectin/kg BW produced >99% efficacy against the susceptible parent strain passaged for 22 generations without any anthelmintic exposure. However, to obtain similar efficacy the moxidectin-selected and the ivermectin-selected strains of H. contortus required 0.05 mg moxidectin/kg BW or 0.4 mg ivermectin/kg BW. These results indicate that development of resistance to one macrocyclic lactone, simultaneously results in resistance to another macrocyclic lactone. However, rates of resistance development differ between compounds and occurs more slowly with moxidectin than with ivermectin.  相似文献   

Impacts of soil fertility management on productivity and economics of rice and fodder intercropping systems under rainfed conditions in Odisha,India          下载免费PDF全文

Dilip Kumar BASTIA  Subrat Kumar BEHERA  Manas Ranjan PANDA 《农业科学学报》2021,20(12):3114-3126

Under small and marginal farm conditions, allocation of land exclusively for forages is not possible. Hence, integration of forages in existing crop geometry can ensure production of grain and fodder, simultaneously under rainfed conditions. A field experiment was conducted to study the effect of different nutrient management practices on rice and fodder intercropping systems under rainfed conditions during 2015–2017. The intercropping system comprised (i) sole rice (R), (ii) rice and cowpea (5:2) (CP) and (iii) rice and ricebean (5:2) (RB) whereas the different nutrient management practices comprised (i) application of farm yard manure (FYM) at 5 t ha^–1 (farmers’ practice) (N₁), (ii) application of inorganic fertilizer (recommended dose of fertilizer (RDF) of rice, 60:30:30 kg ha^–1 of N:P₂O₅:K₂O) (N₂) and (iii) application of both FYM at 5 t ha^–1 and 50% of RDF inorganic fertilizer (N₃). The results of the experiment revealed that the growth attribute such as leaf area was influenced significantly when fodder crops were taken as intercrops because rice plant was getting more nitrogen from soil due to nitrogen fixation of leguminous fodder crops. Among the nutrient management practices, significant differences in leaf area were found beween N₂ and N₁, and between N₃ and N₁ treatments. However, regarding total number of effective tillers, significant differences were found neither between nutrient management practices nor between cropping systems. The rice equivalent yield (REY) based on price (REY_P) was found to be significantly lower in CP (2 615 kg ha^–1; –6.4%) and RB intercropping systems (2 571 kg ha^–1; –8.0%) than in R monocropping system (2 794 kg ha^–1). However, the REY based on energy (REY_E) of CP (2 999 kg ha^–1; +7.3%) and RB (2 960 kg ha^–1; +5.9%) were found to be significantly higher than that of R (2 794 kg ha^–1) irrespective of nutrient management practices. Between different nutrient management practices, the N₃ treatment recorded the highest REY_P and REY_E which was at par with the N₂ treatment and significantly higher than the N₁ treatment irrespective of cropping systems. The combined application of both organic and inorganic sources of nutrients helped to supply nutrients throughout the growing season, which led to improved growth parameters and rice yield. The R monocropping system resulted in more income and rain water use efficiency (RWUE) closely followed by rice and fodder intercropping systems. However, the REY_E and energy use efficiency (EUE) of rice and fodder intercropping systems were higher than those of R. Also, fodder helped to meet the requirement of cattle feeding in the off-season. Hence, the intercropping system is advocated in the study zone. Further study can be done on ecosystem services and carbon sequestration potential of the intercropping system, as well as the system’s coping ability in response to short drought through observing periodic soil moisture regime in root zone.  相似文献   

OGG1 DNA Repair Gene Polymorphism As a Biomarker of Oxidative and Genotoxic DNA Damage     

Kanika Miglani  Sunil Kumar  Anita Yadav  Neeraj Aggarwal  Ranjan Gupta 《Iranian Biomedical Journal》2021,25(1):47

Background:Single nucleotide polymorphisms in OGG1 gene modulates DNA repair capacity and functions as one of the first lines of protective mechanisms against 8-OHdG mutagenicity. OGG1-Cys326 gene polymorphism may decrease DNA repair function, causing oxidative stress due to higher oxidative DNA damage. The main purpose of this study was to examine the link of oxidative and genotoxic DNA damage with DNA repair OGG1 gene polymorphism, in charcoal workers exposed to polyaromatic hydrocarbons. Methods:Urinary 8-OHdG excretion (a biomarker of oxidative DNA damage) was determined in both exposed and control populations. Genotyping of OGG1 DNA repair gene in the blood samples of subjects was carried out by PCR-RFLP method. Results:The 8-OHdG urinary concentration was significantly higher (p < 0.05) in the exposed (geometric mean 12.33 ± 3.78) than in the unexposed (geometric mean 7.36 ± 2.29) population. DNA damage, as measured by 8-OHdG and TM content, was found to be significantly higher in OGG1 homozygous mutants (mt/mt; 18.81 ± 3.34; 6.04 ± 0.52) as compared to wild-type genotypes (wt/wt; 10.34 ± 2.25; 5.19 ± 2.50) and heterozygous (wt/mt) mutants (12.82 ± 2.81; 6.04 ± 0.93) in the exposed group. Conclusion:We found a significant association of OGG1 heterozygous (wt/mt) and homozygous (mt/mt) variants with oxidative and genotoxic damage, suggesting that these polymorphisms may modulate the effects of PAH exposure in occupational workers. Key Words: 8-hydroxy-2’-deoxyguanosine, 1-hydroxypyrene, Polycyclic aromatic hydrocarbons  相似文献   

Genetic relationships of gladiolus cultivars inferred from fluorescence based AFLP markers     

Pragya Ranjan  K.V. Bhat  R.L. Misra  S.K. Singh  J.K. Ranjan 《Scientia Horticulturae》2010

Gladiolus is one of the important commercial flowers with a large number of cultivars. However, genetic relationships among its genotypes have not been reported. This study analyzed genetic relatedness of 54 gladiolus cultivars using amplified fragment length polymorphism (AFLP) markers. A total of 24 AFLP primer pairs with three samples were initially screened, from which 9 primer sets that showed clear scorable and highly polymorphic bands were selected for AFLP reactions. Fluorescence-labeled amplification products were subjected to electrophoresis and then analyzed using an automated sequencer. A dendrogram was constructed by the unweighted pair group method using the arithmetic average (UPGMA). The number of AFLP fragments generated per primer set ranged from 10 to 151 with fragment sizes varying from 50 to 450 bp. A total of 660 AFLP fragments were detected, of which 658 (99.70%) were polymorphic. All the primers except E-AGG/M-CTA displayed 100% polymorphism. All cultivars were clearly differentiated by their AFLP profiles. The AFLP data were compared with previously obtained RAPD data and combined to generate a common dendrogram. The first cluster was dominated with indigenously bred cultivars while the second was dominated with exotic cultivars. This shows that most of the exotic cultivars as well as indigenous cultivars are closely related with each other. However, two indigenous cultivars viz., Pusa Suhagin and Pusa Archana share genetic similarity with exotic cultivars. Among the genotypes selected for the investigation, Pusa Gunjan was identified as the most distinct genotype. The AFLP markers developed will help future Gladiolus cultivar identification, germplasm conservation and new cultivar development. The assessed genetic relationships among gladiolus cultivars may enhance the efficiency of breeding program by selecting desirable parents with reduced breeding cycle.  相似文献   

Effect of vitamin E and selenium supplementation on oxidative stress indices and cortisol level in blood in water buffaloes during pregnancy and early postpartum period   总被引：1，自引：1，他引：0  

Umesh Dimri  Rakesh Ranjan  Mahesh C. Sharma  V. P. Varshney 《Tropical animal health and production》2010,42(3):405-410

Pregnancy is a physiology state accompanied by high energy and oxygen demand that may lead to increased level of oxidative stress and development of metabolic and reproductive disorders in pregnant water buffaloes. In the present study, the alterations in serum cortisol and erythrocyte lipid peroxides and superoxide dismutase activities were examined in 28 pregnant water buffaloes supplemented with antioxidant nutrients, Vitamin E and selenium. Another 14 buffaloes did not receive any treatment during pregnancy to serve as negative control. Results of the present study suggested that pregnancy is associated with oxidative stress and supplementation of vitamin E and selenium may be beneficial by alleviating oxidative stress in water buffaloes.  相似文献