辣椒疫病药剂防治效果及对产量和农艺性状的影响     

彭诗云  刘利  夏兴勇  谢挺  罗燚 《辣椒杂志》2015,13(1)

为筛选出防治辣椒疫病的高效低毒新药剂,以遵辣6号为供试品种,选择68.75%银法利、58%甲霜灵猛锌、69%烯酰吗啉·锰锌、72%霜霉疫净及70%安泰生5种药剂进行防效试验,并对施药后辣椒的农艺性状和产量进行调查。结果表明,68.75%银法利、58%甲霜灵锰锌、69%烯酰吗啉·锰锌、72%霜霉疫净及70%安泰生对辣椒疫病的防治效果分别为80.12%、73.2%、59.84%、40.05%和19.88%,用药后辣椒产量分别比对照增产18.39%、15.33%、13.8%、8.05%和6.51%。这5种药剂的综合表现依次为68.75%银法利58%甲霜灵锰锌69%烯酰吗啉·锰锌72%霜霉疫净70%安泰生。  相似文献   

Transplantation of bcl-2 gene-modified bone marrow mesenchymal stem cells improves cardiac function and angiogenesis in rabbit ischemic car-diac insufficiency model     

GAO Qing  LI Shu-ren  XUN Li-ying  YUAN Ke-xin  XIE Yue-tao  ZHANG Qian-hui  HAO Qing-qing  DANG Yi  QI Xiao-yong 《园艺学报》2015,31(4):640-646

AIM: To investigate the effects of transplantation of bone marrow mesenchymal stem cells (BMSCs) modified by bcl-2 gene on myocardial cell apoptosis, angiogenesis and cardiac function in the rabbit after acute myocardial infarction (MI).METHODS: The rabbit BMSCs were isolated, cultured and purified in vitro. The BMSCs were transfected with adenovirus or adenovirus-Bcl-2. The rabbit model of MI was established by ligation of left anterior descending branch. The rabbits were injected with Ad-Bcl-2-BMSCs (MI+Bcl-2-BMSCs group), Ad-BMSCs (MI+BMSCs group) and DMEM (MI group) in infarction marginal zone 2 weeks after ligation. The cardiac function was evaluated by echocardiography.The apoptosis of myocardial cells was measured by TUNEL. The mRNA expression of VEGF was detected by real-time PCR. The expression of CD31 was examined by immunohistochemical staining, and new blood capillaries were counted at 4 weeks after BMSCs transplantation. The correlation of the above values with cardiac function was analyzed.RESULTS: The cardiac function was better, the apoptotic rate was lower, the mRNA expression of VEGF and the capillary density were higher in both MI+Bcl-2-BMSCs group and the MI+BMSCs group than those in MI group, and those in MI+Bcl-2-BMSCs group increased more obviously.The left ventricular ejection fraction (LVEF) had a negative correlation with the myocardial cell apoptosis rate. A positive correlation with the mRNA expression level of VEGF and the capillary density was also observed.CONCLUSION: The transplantation of BMSCs modified by bcl-2 gene significantly reduces the myocardial cell apoptosis, promotes angiogenesis, improves heart function of the rabbits with MI.  相似文献   

干旱处理对华北绣线菊和毛果绣线菊光合作用及相关蛋白表达的影响     

刘计璇  胡冰  仉茜  苏青  张娇  刘慧民 《园艺学报》2015,42(5):917-923

以抗旱力弱的毛果绣线菊与抗旱力强的华北绣线菊为试验材料,采用不同程度干旱处理,在测定其光合作用变化的基础上,采用蛋白质组学技术研究两种绣线菊光合作用相关蛋白表达的变化。结果表明:干旱胁迫两种绣线菊光合能力降低,光补偿点和光饱和点降低,说明利用弱光能力提高,华北绣线菊适应弱光环境的能力强于毛果绣线菊。不同水平干旱处理分别鉴定出涉及能量代谢与运输的相关蛋白20种。干旱胁迫下毛果绣线菊中卡尔文循环途径相关酶、光系统Ⅱ中多数放氧复合蛋白和捕光色素复合蛋白、参与光合磷酸化的酶发生显著上调和下调表达;华北绣线菊中参与卡尔文循环代谢相关酶、PSⅠ中反应中心蛋白和参与光合电子传递的蛋白、PSⅡ中捕光色素复合蛋白、参与光合磷酸化与氧化磷酸化的蛋白发生明显的上调和下调表达。干旱胁迫使抗旱力不同的毛果绣线菊和华北绣线菊光合作用相关蛋白均受到影响,毛果绣线菊中多是能量代谢相关蛋白发生显著变化,华北绣线菊中多是能量运输相关蛋白发生显著变化。  相似文献   

柑橘新品种‘粤丰早橘’     

姜波  钟云  曾继吾  谢文根  吴大清  易干军  闫化学  钟广炎  陈良周  彭成绩  蔡明段 《园艺学报》2015,42(12):2537-2538

‘粤丰早橘’是从‘南丰蜜橘’芽变中选育出来的优良新品种,具有适应性强,丰产稳产、果实大、化渣性好等特点,平均单果质量58.33 g,可溶性固形物含量13.36%,总糖含量11.80%,总酸含量0.52%。在广东韶关地区种植,果实10月上旬成熟。  相似文献   

毛花猕猴桃新品种‘赣猕6号’     

徐小彪  黄春辉  曲雪艳  陈明  钟敏  郎彬彬  陈楚佳  谢敏  张文标 《园艺学报》2015,42(12):2539-2540

‘赣猕6号’系从野生毛花猕猴桃(Actinidia eriantha)自然变异群体中选育而成的新品种。果实长圆柱形,果面密被白色短茸毛。果实中大,平均单果质量72.5 g,最大单果质量96 g。果肉墨绿,可溶性固形物含量13.6%,可滴定酸含量0.87%,干物质含量为17.3%。维生素C含量7.23 mg·g-1。果实成熟期为10月下旬。果实后熟达食用状态时易剥皮,肉质细嫩清香,风味酸甜适度。  相似文献   

Effects of microglia-derived IL-1β on differentiation of OPCs in corpus callosum of septic neonatal rats     

XIE Di  ZENG Hong-ke  CHEN Chun-bo  DENG Yi-yu 《园艺学报》2015,31(3):385-391

AIM: To explore whether IL-1β inhibits the oligodendrocyte precursor cell (OPCs) differentiation and affects axonal myelination. METHODS: One-day-old SD rats were randomly divided into control group and LPS group (48 rats in each group). The rats in LPS group were intraperitoneally injected with 1 mg/kg LPS. The rats in control group were injected with an equal volume of PBS. The rats in each group were further divided into 3 h, 24 h, 3 d, 7 d, 14 d and 28 d subgroups after injection. The expression of IL-1β and IL-1R₁ in the rat corpus callosum at 3 h, 24 h, 3 d, 7 d was determined by double immunofluorescence and Western blotting. The myelin basic protein(MBP) expression in the rat corpus callosum at 14 d, 28 d after injection was also measured. In vitro, primary OPCs culture was performed and divided into control group, 30 μg/L IL-1β group, 30 μg/L IL-1β+IL-1Ra group and 30 μg/L IL-1Ra group. The expression of MBP in the OPCs induced differentiation for 3 d was observed by double immunofluorescence and Western blotting. RESULTS: The expression of IL-1β and IL-1R₁ in the rat corpus callosum at 3 h, 24 h, 3 d, 7 d after LPS injection was obviously increased and the expression of MBP in the rat corpus callosum at 14 d, 28 d in LPS group was obviously decreased compared with control group in vivo. The level of MBP was significantly decreased after IL-1β treatment for 3 d in vitro. However, IL-1Ra (IL-1R inhibitor) reversed the down-regulation of MBP expression. IL-1β inhibited the expression of p-ERK, ERK over-expression reversed the down-regulation of MBP expression compared with IL-1β group. CONCLUSION: IL-1β inhibits the differentiation of OPCs, which may be involved in ERK pathways, thus leading to axonal hypomyelination in the corpus callosum of septic neonatal rats.  相似文献   

麝香百合×亚洲百合(LA,Longiflorum×Asiatic)百合杂种F_1代镰刀菌抗性的快速鉴定     

罗建让  谢松林 《北方园艺》2015,(6):90-94

以99个百合远缘杂种F1代-麝香百合×亚洲百合(LA)杂种为试材,采用栽培基质接种病原菌的方法对杂种后代的镰刀菌抗性进行了鉴定。结果表明:百合无性系温室人工接种技术成功应用于该远缘杂种,其母本麝香百合‘White Fox’对镰刀菌表现出高感,而父本亚洲百合‘Connecticut King’具有较强抗性,并成功遗传给杂种后代,在后代基因型中实现一系列分离;在后代杂种群体中,大部分基因型对镰刀菌表现出不同程度的抗性,F1代镰刀菌抗性基因表达率为70.1%。表明镰刀菌抗性由数量基因控制,通过杂种系间杂交实现百合镰刀菌抗性的渐渗育种切实可行。  相似文献   

白灵菇品种选育研究   总被引：1，自引：0，他引：1  

徐兵  姚璐晔  朱婧  冀宏 《北方园艺》2015,(6):147-151

以"白灵0号"、"白灵1号"、"白灵2号"3种白灵菇孢子为试材,采用杂交育种及紫外诱变育种技术,在致死率75%条件下进行紫外线诱变处理;以其为亲本,采用单孢杂交(两点法)进行单核菌丝体交配后,观测菌丝的"锁状联合"、拮抗试验(三点法)和酯酶同工酶谱鉴定杂交子。通过杂交子与亲本的菌丝体生长速度、色泽及菌丝体密度对比,筛选出优势菌株26株,选育白灵菇新品种。结果表明:经过诱变处理的白灵菇孢子具有较高杂交亲和性,且杂交子呈现正向变异率高的特点。  相似文献   

不同蓝莓品种的叶片结构和气孔特性比较     

谢兆森  宋世鑫  曹红梅 《北方园艺》2015,(4):5-8

选取3年生"蓝丰"、"北陆"、"美登"蓝莓品种为试验材料,采用石蜡切片法和"指甲油法"观察了3个蓝莓品种叶片解剖结构和气孔特性。结果表明:3个品种的叶片的角质层、表皮细胞、栅栏组织、海绵组织等组织厚度都存在着差异,其中"蓝丰"的角质层、表皮、栅栏组织、海绵组织厚度最大,"北陆"次之,"美登"最小;从叶片组织紧密度来看,"美登"的最大,"北陆"次之,"蓝丰"最小;栅海比参数比较中,"北陆"最大,"美登"次之,"蓝丰"最小;从蓝莓叶片气孔特性来看,"蓝丰"气孔长度和宽度最大,"北陆"次之,"美登"最小,而气孔的密度呈现相反的结果。  相似文献   

基于因子分析的核桃品质综合评价研究     

杨静怡  丁晓霞  夏玉芳  谢钊俊  陶兴月 《北方园艺》2015,(9):28-31

为从众多核桃品质的评价指标中找到主导的影响因子,达到用最少的指标获取最多核桃品质信息的目的,试验以赫章8个单株核桃为试材,通过对表征核桃物理化学特性的12项指标进行测定,利用因子分析,对众多指标进行降维处理,找出少数几个影响核桃品质的主导因子。结果表明:12项原始指标归属为3个相互独立的公因子,累计方差贡献率达到85.960%,其中,出仁率和壳厚度是重要的核桃品质的物理指标,蛋白质含量是重要的化学品质评价指标。核桃的理化品质与亲缘关系之间有一定的联系,利用因子分析结果进行聚类分析发现,1、5、6号核桃理化品质相似,可能具有较近的亲缘关系。4、7、8号单株单独成为3类,说明可能与其它单株的亲缘关系较远。研究结果可在一定程度为核桃资源的品质鉴定、优株鉴选、分类及亲缘关系的确定等提供理论依据。  相似文献