A longitudinal study of verotoxin-producing Escherichia coli in two dairy goat herds     

Orden JA  Cortés C  Horcajo P  De la Fuente R  Blanco JE  Mora A  López C  Blanco J  Contreras A  Sánchez A  Corrales JC  Domínguez-Bernal G 《Veterinary microbiology》2008,132(3-4):428-434

A longitudinal study was conducted on two dairy farms to investigate the pattern of shedding of verotoxin-producing Escherichia coli (VTEC) in goats. Faecal samples were taken from 20 goat kids once weekly during the first 4 weeks of life and then once every month for the next 5 months of life, and from 18 replacement animals and 15 adults once every month for 12 months. The proportion of samples containing VTEC was higher for replacement animals and adults (85.7% and 78.7%, respectively) than for goat kids (25.4%). About 90% of the VTEC colonies isolated from healthy goats belonged to five serogroups (O33, O76, O126, O146 and O166) but the most frequent serogroups of these isolates, except one, were different in the two herds studied. E. coli O157:H7 was found in three goat kids on only one occasion. None of the VTEC isolates, except the three E. coli O157:H7 isolates, was eae-positive. The patterns of shedding of VTEC in goat kids were variable, but, in contrast, most of the replacement animals and adults were persistent VTEC shedders. Our results show that isolates of VTEC O33, O76, O126, O146 and O166 are adapted for colonising the intestine of goats but that, in contrast, infection with VTEC O157:H7 in goats seems to be transient.  相似文献   

Serotypes, virulence genes, intimin types and PFGE profiles of Escherichia coli isolated from piglets with diarrhoea in Slovakia     

Vu-Khac H  Holoda E  Pilipcinec E  Blanco M  Blanco JE  Dahbi G  Mora A  López C  González EA  Blanco J 《Veterinary journal (London, England : 1997)》2007,174(1):176-187

Two hundred and fifty Escherichia coli isolates from diarrhoeic and healthy piglets were serotyped and tested for the presence of virulence genes for fimbriae, intimin, heat-labile (LT) and heat-stable (STa and STb) enterotoxins, Stx toxins, and enteroaggregative heat-stable 1 (EAST1) enterotoxin by polymerase chain reaction (PCR). Although 220 isolates from diarrhoeic piglets belonged to 43 O serogroups and 77 O:H serotypes, 60% were of one of the 10 serogroups O2, O8, O15, O54, O84, O101, O141, O147, O149 and O157, and 60% belonged to only 10 serotypes (O8:H-, O54:H-, O84:H7, O101:H-, O141:H-, O141:H4, O147:H-, O149:H10, O163:H-, and ONT:H-). PCR showed that 79% of 220 isolates carried genes for at least one of the virulence factors tested. The gene encoding for EAST1 was the most prevalent (65%) followed by those encoding for STb (49%), LT (42%), STa (13%), and Stx2e (4%). Eighty-three (38%) of the 220 E. coli isolates carried the gene for F4 (K88), whereas genes for F18, F5 (K99), F41, F6 (P987), F17, and intimin (eae) were detected in 9%, 3%, 3%, 3%, 1%, and 3%, respectively. Seropathotype O149:H10:F4:LT/STb/EAST1 (70 isolates) was the most common, representing 32% of isolates. Pulsed-field gel electrophoresis (PFGE) analysis with XbaI of 15 O149:H10 representative isolates from diarrhoeic piglets distinguished 14 types. The 15 isolates exhibited a wide variability of distinct restriction patterns though all belonged to the same serotype (O149:H10), and all but one showed identical virulence determinants (F4, LT, STb, and EAST1). Among 30 isolates from healthy piglets only two virulence genes were detected: EAST1 (26%) and eae (17%). In total, 12 isolates were positives for the eae gene: five isolates had intimin beta1, four possessed intimin theta and three showed intimin type xiB. This is believed to be the first study describing the presence of intimin type xiB in E. coli of porcine origin.  相似文献   

Determining suitable selection cutting intensities based on long-term observations on aboveground forest carbon,growth, and stand structure in Changbai Mountain,Northeast China     

Yang Liu  Juan A. Blanco  Xiaohua Wei  Weifeng Wang  Yanrong Guo 《Scandinavian Journal of Forest Research》2014,29(5):436-454

We studied the effects of different cutting intensities (0%, 5–10%, 15%, and 20% basal area removal) on stand growth, structure, and net carbon storage in spruce–fir (Picea jezoensis (Sieb. et Zucc.) Carr.–Abies nephrolepis (Trautv.) Maxim.) and broadleaf mixed forests on Changbai Mountain (Northeast China) over 19 years. At this site, inventory-based low-intensity selection cutting was used to maintain a continuous forest canopy. After two cutting events, results showed significant differences in growth, structure, and carbon storage among cutting intensities. When increasing cutting intensity, the growth rate of average diameter, basal area, and volume significantly increased, with the highest increment rates found in the plots with 20% basal area removal. Tree diameters for all plots showed a roughly inverse J-shaped distribution before cutting and a left-skewed unimodal distribution after two cuttings. Volume ratio (the relative amount of volume contained in different diameter classes) for small (6–14 cm), medium (14–26 cm), large (26–36 cm), and very large (>38 cm) diameters remained unchanged in the plots with 5 and 10% basal area removal, but the volume ratio of large and very large diameters increased in the plots with 15 and 20% basal area removal, reaching approximately a 1:2:3:4 ratio in the plots with 20% basal area removal after two selection cuttings. Net carbon storage increased when increasing cutting intensity, reaching maximum storage in the plots with 20% basal area removal (cutting intensity and net C storage increase: 0%, 7.21 Mg C ha^?1, 5–10%: 11.68 Mg C ha^?1, 15%: 21.41 Mg C ha^?1, 20%: 26.47 Mg C ha^?1). Therefore, our results show the potential of low-intensity selection cutting to meet demands of both timber production and maintenance of forest cover for biodiversity values.  相似文献   

Modeling the timber supply impact of introducing weevil-resistant spruce in British Columbia with cellular automata     

Olaf Schwab  Thomas Maness  Gary Bull  Clive Welham  Brad Seely  Juan Blanco 《Forest Policy and Economics》2011,13(1):61-68

Assessing the impacts of cumulative, non-lethal damages to forests poses a particular challenge to forest management planning since cumulative merchantable volume losses are not known until the time of harvest. This paper introduces a model that scales stand-level processes to their large-scale implications by combining elements of growth and yield modeling, insect infestation modeling, and large-scale spatial harvest simulation. The model is described and tested using the proposed large-scale introduction of weevil-resistant spruce in British Columbia, Canada as a case study. While spruce weevil damages were found to be significant, only a relatively small percentage of losses can be avoided by planting resistant stock. The model introduced in this paper can serve as an important decision support tool for policy makers and researchers in assessing the large-scale effects of proposed policy and management interventions.  相似文献   

Lactate Dehydrogenases in Human Testes     

Blanco A  Zinkham WH 《Science (New York, N.Y.)》1963,139(3555):601-602

A unique form of lactate dehydrogenase was observed in the starch-gel electrophoretic patterns of adult human testes. It was present in sperm, but absent in prepubertal testes. Its electrophoretic mobility, heat stability, kinetic behavior with pyridine nucleotide analogs, and chromatographic characteristics on diethylaminoethyl cellulose were intermediate to those observed for lactate dehydrogenase isozymes 3 and 4.  相似文献   

Early assessment of gilthead sea bream (Sparus aurata) spawning dynamics by mini‐broodstocks          下载免费PDF全文

Carmen García‐Fernández  José A Sánchez  Gloria Blanco 《Aquaculture Research》2018,49(1):36-47

The implementation of any sustainable breeding program requires a maximization of effective size (Ne) to maintain inbreeding rate at minimum levels. However, the control of Ne remains a major challenge for those species whose reproduction in captivity is based on a spawning strategy where there is no control over the established matings. The present study aims to assess the spawning dynamic of gilthead sea bream (Sparus aurata) at different dates from two mini‐broodstocks (4 ♀ and 4 ♂). Here we report the use of a simple protocol based on the genotyping of fertilized eggs from a reduced set of microsatellite loci which provides a quick evaluation of daily spawning in terms of family structuring (number of active breeders and number and family size). According to our results, the daily spawning dynamics is characterized by a fluctuation in the number of active breeders, reaching maximum values (until 100%) during peak production phase. However, the proportion of possible mating (families) in a single daily spawning never exceeded more than 81%. Unbalanced parental contributions were highly frequent, with extreme cases involving the assignment of 50%–90% of the offspring to a single family. Therefore, the use of mini‐broodstocks and the grouping of spawning harvested at different dates are proposed as strategies to minimize the inbreeding risk by providing a more optimal pattern of family structuring (increase in the number of families obtained and a more balanced parental contributions) that entails a more conservative Ne/N ratio.  相似文献   

Comparison of the effects of topical application of UMF20 and UMF5 manuka honey with a generic multifloral honey on wound healing variables in an uncontaminated surgical equine distal limb wound model          下载免费PDF全文

AS Tsang  AJ Dart  A Sole‐Guitart  CM Dart  NR Perkins  LB Jeffcott 《Australian veterinary journal》2017,95(9):333-337

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