Effects of Chloroformic Extracts from Washed and Unwashed Papaya Seeds (Carica papaya) on the Sperm Concentration of Dogs     

A Ortega‐Pacheco  M Jiménez‐Coello  E Gutiérrez‐Blanco  KY Acosta‐Viana  E Guzmán‐Marín  MA Zavala‐Sánchez  NE Montalvo‐Beltrán  MS Pérez‐Gutiérrez 《Reproduction in domestic animals》2010,45(6):1126-1129

Papaya seeds (Carica papaya Linn) have been found to have a significant effect on sperm characteristics in some mammals, including humans, but no studies have investigated the effects on dogs. In the present study, a significant decrease in sperm concentration was observed in a group of dogs treated with extracts from washed papaya seeds, but no decrease was observed in the group of dogs treated with non‐washed seeds. An important effect of extract components from washed seeds such as fatty acids is probably involved in the reduction of sperm production because of Sertoli cell damage, as has been suggested for langur monkeys. Dilution of the active components in the non‐washed papaya seeds or interference with some of the components may reduce the expected effect on spermatogenesis. This first report on the effects of a chloroformic extract of papaya seeds in dogs suggests that an increased dose is necessary to achieve azoospermia.  相似文献   

Aromatic characterization of pot distilled kiwi spirits     

López-Vázquez C  García-Llobodanin L  Pérez-Correa JR  López F  Blanco P  Orriols I 《Journal of agricultural and food chemistry》2012,60(9):2242-2247

This study contributes fundamental knowledge that will help to develop a distillate of kiwi wine, made from kiwis of the Hayward variety grown in the southwest of Galicia (Spain). Two yeast strains, L1 (Saccharomyces cerevisiae ALB-6 from the EVEGA yeast collection) and L2 (S. cerevisiae Uvaferm BDX from Lallemand) were assessed to obtain a highly aromatic distillate. The kiwi spirits obtained were compared with other fruit spirits, in terms of higher alcohols, minor alcohols, monoterpenols, and other minor compounds, which are relevant in determining the quality and taste of the kiwi spirits. It was found that the kiwi juice fermented with yeast L1 produced a more aromatic distillate. In addition, kiwi distillates produced with both yeasts had the same ratio of trans-3-hexen-1-ol and cis-3-hexen-1-ol, which is lower than that found in other fruit distillates.  相似文献   

Effects of topical treatment of cannabidiol extract in a unique manuka factor 5 manuka honey carrier on second intention wound healing on equine distal limb wounds: a preliminary study     

VC McIver  AS Tsang  NE Symonds  NR Perkins  E Uquillas  CM Dart  LB Jeffcott  AJ Dart 《Australian veterinary journal》2020,98(6):250-255

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Genetic diversity in Borago officinalis germplasm as revealed by seed oils and AFLP polymorphism     

Antonino De Lisi  Vincenzo Montesano  Donatella Negro  Giulio Sarli  Emanuela Blanco  Gabriella Sonnante  Gaetano Laghetti 《Genetic Resources and Crop Evolution》2014,61(4):853-859

Ten accessions of wild Borago officinalis were collected during missions in the course of a research activity carried out on plants producing nutraceutical compounds. The aim was to identify populations that produce a higher content of γ-linolenic acid, as this compound has important therapeutic properties and has been used in medical tests to treat rheumatoid arthritis, eczema, and psoriasis with good therapeutical results. The present study reports on the characterization of both the polyunsaturated fatty acid composition and on genetic diversity estimated by means of AFLP markers in several B. officinalis accessions collected in Southern Italy and grown in isolation by means of screen houses specifically designed for each single population. Polyunsaturated fatty acid were found in different amounts in all investigated accessions. The populations were very variable at the molecular level, and the analysis of relationships based on AFLP data, showed that similarity based on Jaccard’s index ranged between 0.330 and 0.742. Our investigations allowed us to identify a population that may be considered as a good source of GLA.  相似文献   

Study of gene effects for cotton yield and Verticillium wilt tolerance in cotton plant (Gossypium hirsutum L.)     

A. Aguado  B. De Los SantosC. Blanco  F. Romero 《Field Crops Research》2008

Verticillium wilt (VW), caused by Verticillium dahliae Kleb., is a destructive disease of cotton (Gossypium hirsutum L.). The use of resistant cultivars has long been considered the most practical and effective mean of control. The aim of this work was to study the quantitative genetic basis of Verticillium wilt resistance in Upland cotton by using five genotypes and their possible crosses without reciprocals selecting simultaneously for resistance and desirable agronomic characteristics. Five cotton cultivars and 10 F1s from half-diallel crosses were analyzed for VW resistance. The seed cotton yield, the number of bolls/ plant, and boll weight were measured and Verticillium wilt index (VWI) was estimated during two crop seasons in two different sites each year always on plots with naturally infested soil. Genetic components of variance were analyzed using the Hayman model. Analysis of variance for all characters showed significant differences between genotypes, without genotype-site interaction in most cases. Both, additive genetic variance component (D) and dominance genetic variance components (H1 and H2) were presented in all characters, except for VWI. D was the most important component for boll weight and VWI. Boll weight was the most correlated character with seed cotton yield and VWI. Broad sense heritability was high for boll weight and VWI, moderate for seed cotton yield and low for bolls per plant. Narrow sense heritability was moderate for boll weight, and high for VWI.  相似文献   

Subtypes of intimin among non-toxigenic Escherichia coli from diarrheic calves in Brazil.          下载免费PDF全文

L Aidar  A S Penteado  L R Trabulsi  J E Blanco  M Blanco  J Blanco    A F Pestana de Castro 《Canadian journal of veterinary research》2000,64(1):15-20

One hundred and five strains of Escherichia coli that were isolated from calves with diarrhea in the state of São Paulo, Brazil, and were negative for enterotoxins and cytotoxins, were examined for the eae gene. Four (3.8%) strains were positive by polymerase chain reaction (PCR) and were shown to produce intimin by using Western blot with specific antiserum against the conserved N-terminal region of intimin. Subtyping of the intimins was done by PCR with specific primers and by Western blot with specific antisera against the C-terminal variable region of the protein. Three of these isolates (O?:H11, O26:H-, O123:H1) produced the beta subtype of intimin, and the 4th (0103:H2) produced intimin that was not typable. The 0103:H2 and the O26:H-isolates adhered to HEp-2 cells with diffuse adherence and localized-like adherence patterns, respectively. The other strains did not adhere to HEp-2 cells. To our knowledge, this is the first report of the occurrence of a subtype of intimin described for human enteropathogenic E. coli among bovine diarrheogenic E. coli. It is also the first report from Brazil demonstrating the presence of bovine E. coli harboring the eae gene.  相似文献   

Fruit characterization of Cocos nucifera L. (ARECACEAE) cultivars from the Pacific coast of Costa Rica and the Philippines     

Alfonso Vargas  Fabio A. Blanco 《Genetic Resources and Crop Evolution》2000,47(5):483-487

Tall coconut cultivars from the Pacific coast of Costa Rica and the Philippines (San Ramón, Tagnanán, and Laguna), were evaluated for fruit characteristics. Most of the introduced cultivars showed extremely large heterogeneity. A cluster analysis, based on the Ward method, classified the palms into four groups with high internal homogeneity. Some of the evaluated coconut palms from the Costa Rican Pacific area had nut characteristics similar to the San Ramon and Tagnanan palm groups but not to the Laguna group. At the association level used (semipartial R ² = 0.10), another group which included the remaining palms sampled from the Costa Rican Pacific coast was constituted.  相似文献   

A spline polynomial model to describe serum IGF-I concentration from birth to slaughter in calves: effects of weaning age,pre-weaning concentrate feeding and breed     

M. Blanco  I. Casasús  D. Villalba 《Domestic animal endocrinology》2010

The statistical analysis of hormones sampled throughout the production cycle is complicated because factors such as age and weight at the measuring date interfere. Spline curves constructed from pieces of low-degree, random-effects polynomials could be used for a more accurate analysis of data. Concentration of insulin-like growth factor-1 (IGF-1), weight gain, and concentrate intake of Parda de Montaña (PM) (n = 27) and Pirenaica calves (n = 14) were modeled with a spline model according to age at weaning, pre-weaning concentrate feeding, and breed. At birth, calves were randomly assigned to early weaning (EW) at 90 d or traditional weaning (TW) at 150 d. During lactation, half of PM calves received concentrates (S), whereas the remainder received no concentrates (NS). After weaning, calves received concentrates on an ad libitum basis until they reached a weight of 450 kg. The spline model had better likelihood than a polynomial of 6 degrees or a split-plot model. Serum IGF-1 concentration was greatly affected by age at weaning and pre-weaning concentrate feeding, but not by breed. In NS calves, IGF-1 concentration was greater in EW than in TW calves from 120 to 300 d, irrespective of breed. During lactation, S calves had greater IGF-1 concentration than NS calves. After weaning, EWNS calves reached the IGF-1 concentration of EWS calves after 4 mo on concentrates, whereas TWNS calves attained IGF-1 concentration of TWS calves after only 2 mo, because of their increased concentrate intake relative to TWS calves. Concentration of IGF-1 was positively correlated with the immediate weight gains and intake, but it was not a good predictor of performance in the long term.  相似文献   

Effect of probiotic Enterococcus faecium SF68 on liver function in healthy dogs     

Rosario Lucena  Manuel Novales  Beatriz Blanco  Eduardo Hernndez  Pedro Jos Ginel 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2019,33(6):2628-2634

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Risk assessment and surveillance for bovine spongiform encephalopathy (BSE) in Argentina   总被引：7，自引：0，他引：7  

A. A. Schudel  B. J. Carrillo  E. L. Weber  J. Blanco Viera  E. J. Gimeno  C. van Gelderen  E. Ulloa  A. Nader  B. G. Can  R. H. Kimberlin 《Preventive veterinary medicine》1996,25(3-4):271-284

An assessment was made of the risks of bovine spongiform encephalopathy (BSE) occurring in Argentina. Most of the factors associated with the origin and development of the BSE epidemic in the UK are essentially absent. For example, Argentina's large sheep and cattle industries are based on low-cost production systems using grass. Concentrated feeds are not used for sheep, rarely for beef cattle and to a comparatively modest extent for dairy cows. Particularly important are the facts that scrapie (and BSE) has never been reported in Argentina—very small amounts of waste tissues from sheep are rendered to produce meat and bone meal (MBM)—and MBM is not used in concentrated feeds for cattle. We conclude that Argentina has an exceptionally low risk of BSE due to scrapie. There is a very small risk of BSE having been introduced via live animals imported from countries with BSE, but this could only give rise to isolated cases because MBM is not fed to cattle.

A surveillance programme has been carried out based largely on a histological examination of brains from three categories of old dairy cows: animals reported on the suspicion of having neurological disease; animals in poor condition at slaughter; healthy animals randomly selected in the abattoir. No evidence of transmissible spongiform encephalopathy was seen in several sections from each of a total of 1019 brains. We conclude that, for most practical purposes, Argentina may be considered to be free from BSE.  相似文献