A DNA Probe for Rapid Screening of Sugar Beet (Beta vulgaris L.) Carrying Extra Chromosomes from Wild Beets of the Procumbentes Section     

C. Jung  R. G. Herrmann 《Plant Breeding》1991,107(4):275-279

Genes from wild species of the Procumbentes section can be transferred to sugar beet chromosomes via translocations. Since large translocations, including for example a gene for nematode resistance, generally result in meiotic disturbances, there is a need to select new diploid resistant beets from progenies of monosomic wild beet addition lines. A dispersed repetitive DNA probe, which is closely correlated with the resistance gene and hybridizes exclusively with wild beet DNA, appears to be highly superior to selection based on isozyme markers. Characteristic ‘fingerprints’ on the available monosomic addition lines reveal the existence of at last 5 different chromosomes in the wild species each housing a gene for nematode resistance. This probe can be used advantageously to identify individuals carrying the intact or fragmented wild beet chromosomes, or even various amounts of translocated chromatin. Strategies are discussed for the identification of new translocation types using straightforward squash dot or Southern hybridization techniques in combination with the wild beet DNA probe.  相似文献   

Influence of cyto‐sterility sources of female line on seed quality of Indian mustard (Brassica juncea L. Czern & Coss.) in relation to storage period          下载免费PDF全文

Shyamal Kumar Chakrabarty  Aniruddha Maity  Jung Bahadur Yadav 《Plant Breeding》2015,134(3):333-337

Effect of cyto‐sterility sources on morphology and yield attributes in F₁ has well been studied in different crops, but not on the seed quality of hybrid. Six cytoplasmic male sterile (CMS) lines, viz. Ogura, Siifolia, Erucoides, Moricandia, Tournefortii and Oxyrrhina, which represent six different sources of CMS, were pollinated by a single maintainer line (‘Pusa Bold’) to check whether CMS sources have any marked effect on seed quality. Seeds were collected in 2007–2008 (rabi season: October–April) from CMS maintenance plot and kept in ambient storage for next 3 years. The results indicated that CMS/cyto‐sterility sources influenced the seed quality parameters in fresh seed as well as after storage. Per cent germination (as means over the storage period) was recorded up to 89.00, 88.25 and 87.88 in Moricandia, Ogura and Oxyrrhina systems, respectively, and it was significantly different from Tournefortii and ‘Pusa Bold’ (70.13 and 74.38, respectively). The CMS sources also had pronounced effect on other seed quality traits, viz. root and shoot length and seedling dry matter, and Ogura and Moricandia (green) performed better.  相似文献   

Color characteristics of monascus pigments derived by fermentation with various amino acids     

Jung H  Kim C  Kim K  Shin CS 《Journal of agricultural and food chemistry》2003,51(5):1302-1306

Various pigment colors were produced by Monascus fermentations with separate addition of 20 amino acids. The color characteristics and structures of the pigment derivatives were investigated. When each amino acid was added to the fermentation broth as a precursor, pigment extracts with different hue and chroma values were obtained depending on the content ratios of yellow, orange, and red colors in the fermentation broth. The yellow and orange pigments were identical regardless of amino acid addition. The red compounds varied on the basis of the type of amino acid added. LC-MS and (1)H and (13)C NMR structural analyses confirmed that the derivative pigments contained the moieties of the added amino acids. L, a, and b values of the CIELAB color system for the derivative pigments were measured. Values of hue and chroma were then calculated. The colors of the derivative pigments were in the range of orangish red to violet red. The hydrophilicities/hydrophobicities of the derivative pigments could be predicted from their log P values, which were estimated using computer programs.  相似文献   

Measurement of Atmospheric Formaldehyde and Monoaromatic Hydrocarbons using Differential Optical Absorption Spectroscopy during Winter and Summer Intensive Periods in Seoul, Korea     

Chulkyu Lee  Young Joon Kim  Sang-Bum Hong  Hanlim Lee  Jinsang Jung  Yeo-Jin Choi  Jungeun Park  Ki-Hyun Kim  Jai-Hoon Lee  Ki-Joon Chun  Hyun-Ho Kim 《Water, air, and soil pollution》2005,160(1-4):181-195

Ozone effects on Mediterranean forests were studied for 10 years in southern France. The aim of these studies was to understand the impact of the pollutant on foliar damage among Aleppo pine (Pinus halepensis Mill) and Arolla pine (Pinus cembra L) populations. During the summers of 2001 and 2002, ozone concentrations were measured using UV absorption analysers (4) and passive samplers (29) at two forest monitoring sites: Site 1 in the Nice coastal area for Aleppo pine and Site 2 in the Mercantour mountains for Arolla pine, in the vicinity of tree observation sites. AOT 40 values were quite high (25–33 ppm.h), especially in the Mercantour mountains, for the 5 months of measurement. Foliar damage (defoliation and discoloration) and specific symptoms (chlorotic mottle) were observed on 8 Aleppo pine and 13 Arolla pine plots. For Arolla pines, there was a significant correlation between defoliation and chlorotic mottles. There was also a correlation between mean ozone concentrations measured by passive samplers and chlorotic mottles. These correlations were less significant for Aleppo pine.  相似文献   

Study of interactions between food phenolics and aromatic flavors using one- and two-dimensional (1)H NMR spectroscopy     

Jung DM  de Ropp JS  Ebeler SE 《Journal of agricultural and food chemistry》2000,48(2):407-412

Changes in flavor release and aroma characteristics in a medium including food phenolics may be attributed to an intermolecular interaction between flavor compounds and phenolics. To investigate the interaction, one- and two-dimensional NMR studies have been carried out on the binding of two phenolics, gallic acid and naringin, with three aroma compounds, 2-methylpyrazine, vanillin, and ethyl benzoate. Evaluation of thermodynamic parameters and intermolecular nuclear Overhauser effects reveals that gallic acid can interact more strongly with aromatic flavors than naringin. The supramolecular complexation is also dependent on the structural nature of the flavors, with 2-methylpyrazine and vanillin interacting more strongly than ethyl benzoate. The interaction is principally pi-pi stacking between the galloyl ring and the aromatic ring of the aroma compounds, but secondary hydrogen-bonding effects help to stabilize the complex and enhance the specificity.  相似文献   

CX3CR1-mediated dendritic cell access to the intestinal lumen and bacterial clearance   总被引：3，自引：0，他引：3  

Niess JH  Brand S  Gu X  Landsman L  Jung S  McCormick BA  Vyas JM  Boes M  Ploegh HL  Fox JG  Littman DR  Reinecker HC 《Science (New York, N.Y.)》2005,307(5707):254-258

Dendritic cells (DCs) and macrophages are critical to innate and adaptive immunity to the intestinal bacterial microbiota. Here, we identify a myeloid-derived mucosal DC in mice, which populates the entire lamina propria of the small intestine. Lamina propria DCs were found to depend on the chemokine receptor CX3CR1 to form transepithelial dendrites, which enable the cells to directly sample luminal antigens. CX3CR1 was also found to control the clearance of entero-invasive pathogens by DCs. Thus, CX3CR1-dependent processes, which control host interactions of specialized DCs with commensal and pathogenic bacteria, may regulate immunological tolerance and inflammation.  相似文献   

Techview: drug discovery. Integrating combinatorial synthesis and bioassays     

Rademann J  Jung G 《Science (New York, N.Y.)》2000,287(5460):1947-1948

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Peptide processing and targeting in the neuronal secretory pathway   总被引：14，自引：0，他引：14  

L J Jung  R H Scheller 《Science (New York, N.Y.)》1991,251(4999):1330-1335

The abdominal ganglion of the marine mollusk Aplysia contains a pair of identified neuronal clusters, the bag cells, which control egg laying by means of a number of unique regulatory mechanisms. Each neuron in the bag cell clusters synthesizes several peptides derived from a single prohormone and packages them into separate vesicles. These vesicles are then differentially localized in specific neuronal processes, thus segregating peptides destined for autocrine and hormonal release sites. Therefore in this system, protein trafficking through the secretory pathway organizes multiple peptide neurochemical messengers to efficiently regulate simple behaviors.  相似文献   

Evidence of a pluripotent human embryonic stem cell line derived from a cloned blastocyst     

Hwang WS  Ryu YJ  Park JH  Park ES  Lee EG  Koo JM  Jeon HY  Lee BC  Kang SK  Kim SJ  Ahn C  Hwang JH  Park KY  Cibelli JB  Moon SY 《Science (New York, N.Y.)》2004,303(5664):1669-1674

Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells.  相似文献   

Diversity and extinction of tropical american mollusks and emergence of the isthmus of panama     

Jackson JB  Jung P  Coates AG  Collins LS 《Science (New York, N.Y.)》1993,260(5114):1624-1626

The gradual closure of the Panamanian seaway and the resulting environmental change stimulated an increase in Caribbean molluscan diversity rather than the mass extinction hypothesized previously on the basis of inadequate data. Upheaval of molluscan faunas did occur suddenly throughout tropical America at the end of the Pliocene as a result of more subtle, unknown causes. There is no necessary correlation between the magnitude of regional shifts in abiotic conditions and the subsequent biological response.  相似文献