The incidence of Dictyocaulus viviparus infections in cattle in The Netherlands. I. The Enzyme Linked Immunosorbent Assay as a diagnostic tool     

J H Boon  A Kloosterman  R van den Brink 《The Veterinary quarterly》1982,4(4):155-160

The Enzyme Linked Immunosorbent Assay (ELISA) provides a very efficient technique for detecting antibodies against Dictyocaulus vivparus in calves. Although low level cross reactions were found in animals with gastrointestinal nematodes, the specificity and sensitivity of the technique are sufficient for herd diagnosis of lungworm infections and for survey work. This conclusion is reached on the basis of artificially and naturally infected calves. ELISA titres correlate well with Indirect Haemagglutination titres, parasitological findings, and clinical observations.  相似文献   

Hidden defects in pets: the newest law     

Boon D 《Tijdschrift voor diergeneeskunde》1999,124(7):216-217

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Plasma granulocyte colony-stimulating factor concentrations in neutropenic, parvoviral enteritis-infected puppies     

Cohn LA  Rewerts JM  McCaw D  Boon GD  Wagner-Mann C  Lothrop CD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1999,13(6):581-586

We evaluated the temporal relationship between neutrophil numbers and plasma granulocyte colony-stimulating factor (G-CSF) concentrations in dogs infected with canine parvovirus, a common infectious cause of neutropenia. G-CSF is produced in response to neutropenia, infection, or inflammation, and results in the production and release of neutrophils from the bone marrow. Adequate numbers of functional neutrophils are necessary for protection from infection, and the timely production of G-CSF is a crucial response to certain diseases. The relationship between peripheral neutrophil numbers and plasma G-CSF concentrations during the course of an infectious disease characterized by neutropenia has not been described previously in dogs. Eight mixed-breed puppies were given an oronasal challenge with canine parvovirus, and peripheral neutrophil numbers as well as plasma G-CSF concentrations were measured daily. G-CSF was not detectable in plasma of any dog before the onset of neutropenia, but G-CSF became detectable just after the onset of neutropenia in the 7 dogs that developed clinical illness. Neutropenia persisted or worsened for at least 2 days after plasma G-CSF became detectable in all 7 dogs. Neutrophil nadir, the highest plasma G-CSF concentrations, and the most severe clinical illness occurred concurrently in most dogs. Although 1 dog died while still neutropenic, plasma G-CSF concentrations declined before resolution of neutropenia in the other 6 dogs, and were again below the limits of detection in 5 of the 6 dogs at the time of resolution.  相似文献   

‘Hollow noses’ in hyacinth,a disorder caused by the herbicide chlorpropham     

P. J. Muller  J. van der Boon 《European journal of plant pathology / European Foundation for Plant Pathology》1972,78(5):198-203

A malformation in hyacinths, first observed in recent years, was found to be an effect of uptake by the roots of the herbicide chlorpropham. The risk of this malformation is even present when normal doses of this compound are used for weed killing, especially on sandy soils with very little organic matter and under conditions of shallow planting.Samenvatting De laatste jaren wordt in hyacinten een onbekende afwijking gevonden. In de neus van de bol bevindt zich een grotere of kleinere holte, vandaar de naam holle neuzen (Fig. 1). De bollen zijn niet rond zoals gezonde, maar aan één zijde, naar de bolschijf toe, afgeplat. In de rokken komen op die plaats talloze, glazige vlekjes voor, waardoor het weefsel een crème-achtige kleur heeft. Ernstig beschadigde bollen hebben een krans van klisters (Fig. 1 en 2). In het veld zijn vóór de oogst al afwijkingen in de bol te vinden. Wanneer beschadigde bollen in de herfst opnieuw worden geplant, ontstaan planten met verscheidene spruiten, zgn. bosjesplanten.Het verschijnsel wordt vooral waargenomen op zeer humusarme duinzandgronden (spuittuinen). De meeste klachten over holle neuzen zijn pas de laatste jaren ontvangen, min of meer sedert het ogenblik dat het machinaal planten sterk is toegenomen. In veldproeven en een potproef werd aangetoond, dat deze afwijking een gevolg is van het door de wortels opnemen van het onkruidbestrijdingsmiddel chloorprofam (Tabel 1 en 2). Het middel kan de wortels gemakkelijk bereiken op zeer humusarme grond en ook als de bollen ondiep of onregelmatig zijn geplant, zoals het geval kan zijn bij machinaal planten. De bol komt dan vaak niet met de bolschijf naar beneden gekeerd in de grond terecht. In dat geval groeien de wortels eerst omhoog in plaats van naar beneden, waardoor de kans op een rechtstreeks contact met het gebruikte herbicide sterk toeneemt.  相似文献   

M-MODE ECHOCARDIOGRAPHY AS A DIAGNOSTIC AID FOR FELINE CARDIOMYOPATHY     

Susan F.  Soderberg  DVM  June A.  Boon  BA  Wayne E.  Wingfield  DVM  MS  Charles W.  Miller  PhD 《Veterinary radiology & ultrasound》1983,24(2):66-73

Normal cats and cats with congestive cardiomyopathy (CCM) and hypertrophic cardiomyopathy (HCM) were examined using M-mode echocardiography to determine its diagnostic capabilities. Sixteen normal cats were examined to verify previously reported data and to add further echocardiographic inforamtion (left atrial/aortic root ratio, left posterior wall thickness at end systole and end diastole, amplitude of mitral valve excursions, and velocity of valve opening and closure) to aid in differential diagnosis. Significant (p<0.05) changes were detected between the normal cats and those with cardiomyopathy. In each type of cardiomyopathy, alterations in left atrial dimension, left atrial/aortic root ratio, left ventricular dimension, left ventricular wall thickness and percentage of ventricular dimensional change were identified. Altered mitral valve motion was found with HCM. Echocardiography was found to be an accurate technique for definitive diagnosis of feline cardiomyopathy.  相似文献   

Describing management attitudes to guide forest policy implementation     

Tove Enggrob Boon  Henrik Meilby 《Small-Scale Forestry》2007,6(1):79-92

Forest policy in Denmark aims to increase the environmental values of forests. For policy implementation it is essential to know how to motivate private owners. Based on a survey among private forest owners in Denmark, four types of owners have been identified, clustered according to their forest management attitudes and practices: (1) the production-oriented owner, (2) the classic forest owner, (3) the environmental/recreational owner, and (4) the indifferent forest owner. Owners in Clusters 1 and 2 are mainly motivated by financial and wood production aspects, whereas owners in Cluster 3 are to a greater extent motivated by environmental and recreational aspects. Cluster 4 is the least motivated cluster. For effective policy intervention, the clusters should be addressed by different means. Owners in Clusters 1 and 2 should be met on their agricultural-production logic, Cluster 3 on their interest to improve environmental values, whereas owners in Cluster 4 might mainly be interested in passive nature management solutions.  相似文献   

Chronic eosinophilic dermatitis associated with persistent feline herpes virus infection in cheetahs (Acinonyx jubatus)     

Munson L  Wack R  Duncan M  Montali RJ  Boon D  Stalis I  Crawshaw GJ  Cameron KN  Mortenson J  Citino S  Zuba J  Junge RE 《Veterinary pathology》2004,41(2):170-176

A chronic ulcerative and eosinophilic dermatitis occurred in 20 captive cheetahs (Acinonyx jubatus) with persistent feline herpes virus 1 (FHV1) infection. Affected animals had erythematous, ulcerated plaques primarily on the face and forelegs in sites of contact with lachrymal and salivary secretions. The dermatitis was characterized by dense infiltrates of eosinophils and plasma cells and pseudoepitheliomatous hyperplasia. Rare keratinocytes within the lesions had nuclei with marginated chromatin and small eosinophilic inclusions composed of herpes virus nucleocapsids. Virus isolated from lesions was confirmed to be FHV1. Lesions persisted and progressed unless removed by cryoexcision. The occurrence of this unusual reaction to FHV1 in approximately 5% of captive North American cheetahs suggests a species propensity for a Th2-dominant response to herpes virus infection. This atypical immune reaction may indicate a heritable trait or modulation of the immune response by other factors such as chronic stress.  相似文献   

Development of a Chlamydophila psittaci species-specific and genotype-specific real-time PCR     

Geens T  Dewitte A  Boon N  Vanrompay D 《Veterinary research》2005,36(5-6):787-797

A Chlamydophila psittaci species-specific real-time PCR targeting the rDNA ribosomal spacer was developed as well as a genotype-specific real-time PCR targeting the Cp. psittaci outer membrane protein A (ompA) gene. The SYBR Green-based species-specific real-time PCR detected Cp. psittaci genotypes A to F, and the recently discovered E/B genotype. The genotype-specific real-time PCR could easily distinguish genotypes C, D, F by use of TaqMan probes. Genotypes A, B and E could not be distinguished from each other by simply using TaqMan probes. For this purpose, non-fluorescent competitor oligonucleotides, had to be used next to the TaqMan probes. Genotype E/B could only be detected by use of a minor groove binder (MGB) probe. Both real-time PCR assays allowed reproducible, sensitive (10 rDNA or ompA copies/microL DNA extract) and specific detection of Cp. psittaci DNA. The genotype-specific real-time PCR was compared to ompA sequencing and ompA restriction fragment length polymorphism (RFLP) analysis using five Cp. psittaci field isolates (99, 61/8, 7344/2, 8615/1 and 7778B15) each consisting of two different genotypes. The currently developed real-time PCR assays were used in a case study on a veterinary school and a turkey farm. In the veterinary school, Cp. psittaci genotypes D, E/B and F infection were detected in all five groups of turkeys, and one veterinarian who was taking care of all these turkeys. On the turkey farm, the presence of two Cp. psittaci genotype B infection waves was demonstrated in one randomly selected turkey, the first wave at the age of 6 weeks, and the second at the age of 12 weeks.  相似文献   

Streptococcus gallolyticus subsp. pasteurianus septicaemia in goslings     

Barnett J  Ainsworth H  Boon JD  Twomey DF 《Veterinary journal (London, England : 1997)》2008,176(2):251-253

Increased mortality was observed in a group of 2-3-week old goslings. At post mortem examination, gross lesions included congestion and haemorrhages in the viscera. Histopathological lesions included generalised congestion of tissues with multifocal bacterial colonies, an acute septic fibrinoid splenitis, and an acute septic hepatitis, all consistent with acute septicaemia. Gram-stained sections of liver and lung confirmed the presence of Gram-positive bacteria and Streptococcus gallolyticus subsp. pasteurianus (Streptococcus bovis biotype II/2) was isolated in pure growth from two of the goslings, and in mixed growth from a third.  相似文献   

Theory and practice to conserve freshwater biodiversity in the Anthropocene     

Rebecca Flitcroft  Michael S. Cooperman  Ian J. Harrison  Diego Juffe‐Bignoli  Philip J. Boon 《水产资源保护:海洋与淡水生态系统》2019,29(7):1013-1021

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