Clinical characteristics and mode of inheritance of familial focal seizures in Standard Poodles     

Licht BG  Lin S  Luo Y  Hyson LL  Licht MH  Harper KM  Sullivan SA  Fernandez SA  Johnston EV 《Journal of the American Veterinary Medical Association》2007,231(10):1520-1528

OBJECTIVE: To determine clinical characteristics and mode of inheritance of seizures in a family of Standard Poodles. DESIGN: Case series. ANIMALS: 90 Standard Poodles descended from the same maternal bloodline (30 with probable idiopathic epilepsy [PIE] and 60 without any history of seizures). PROCEDURES: Researchers contacted owners to determine whether dogs had ever had any seizures and, if so, the nature of any such seizures and any potential underlying causes. Dogs were considered to have PIE if they were between 6 months and 7.5 years old at the time of seizure onset and had no evidence of any underlying cause. To determine the mode of inheritance, segregation analyses were designed to allow the family to be analyzed as a whole, as opposed to as nuclear families. Competing models of inheritance were compared statistically for their ability to explain the data. RESULTS: Of the dogs with PIE, 28 (93%) had focal onset seizures with or without secondary generalization. Median age of onset was 3.7 years; 6 dogs were > 5 years old at the onset of seizures. Segregation analyses strongly suggested that PIE was inherited as a simple recessive autosomal trait with complete or almost complete penetrance. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that in this family of Standard Poodles, PIE was inherited as a simple recessive autosomal trait with complete or almost complete penetrance. Seizures often had focal, as opposed to generalized, onsets, and it was not uncommon for seizures to begin after 5 years of age.  相似文献   

Efficacy, duration, and onset of immunogenicity of a West Nile virus vaccine, live Flavivirus chimera, in horses with a clinical disease challenge model   总被引：1，自引：0，他引：1  

Long MT  Gibbs EP  Mellencamp MW  Bowen RA  Seino KK  Zhang S  Beachboard SE  Humphrey PP 《Equine veterinary journal》2007,39(6):491-497

REASON FOR PERFORMING STUDY: West Nile virus (WNF) is a Flavivirus responsible for a life-threatening neurological disease in man and horses. Development of improved vaccines against Flavivirus infections is therefore important. OBJECTIVES: To establish that a single immunogenicity dose of live Flavivirus chimera (WN-FV) vaccine protects horses from the disease and it induces a protective immune response, and to determine the duration of the protective immunity. METHODS: Clinical signs were compared between vaccinated (VACC) and control (CTRL) horses after an intrathecal WNV challenge given at 10 or 28 days, or 12 months post vaccination. RESULTS: Challenge of horses in the immunogenicity study at Day 28 post vaccination resulted in severe clinical signs of WNV infection in 10/10 control (CTRL) compared to 1/20 vaccinated (VACC) horses (P<0.01). None of the VACC horses developed viraemia and minimal histopathology was noted. Duration of immunity (DPI) was established at 12 months post vaccination. Eight of 10 CTRL exhibited severe clinical signs of infection compared to 1 of 9 VACC horses (P<0.05). There was a significant reduction in the occurrence of viraemia and histopathology lesion in VACC horses relative to CTRL horses. Horses challenged at Day 10 post vaccination experienced moderate or severe clinical signs of WNV infection in 3/3 CTRL compared to 5/6 VACC horses (P<0.05). CONCLUSIONS: This novel WN-FV chimera vaccine generates a protective immune response to WNV infection in horses that is demonstrated 10 days after a single vaccination and lasts for up to one year. POTENTIAL RELEVANCE: This is the first USDA licensed equine WNV vaccine to utilise a severe challenge model that produces the same WNV disease observed under field conditions to obtain a label claim for prevention of viraemia and aid in the prevention of WNV disease and encephalitis with a duration of immunity of 12 months.  相似文献   

An 8bp indel in exon 1 of Ghrelin gene associated with chicken growth   总被引：7，自引：0，他引：7  

Fang M  Nie Q  Luo C  Zhang D  Zhang X 《Domestic animal endocrinology》2007,32(3):216-225

Ghrelin, acts as the endogenous ligand for growth hormone secretagogues receptor (GHS-R), is a novel growth hormone (GH) releasing peptide with reported effects on food intake in chickens. In this study, an 8 bp indel polymorphism in exon 1 of the chicken Ghrelin (cGHRL) gene was genotyped in a F(2) designed full-sib population to analyze its associations with chicken growth and carcass traits. Later, mRNA level in the proventriculus was determined by real-time PCR to reveal the expression feature of cGHRL gene. Result showed that this 8 bp indel was significantly associated with body weight at the age of 28 days (BW28) and 56 days (BW56), eviscerated weight (EW) and leg muscle weight (LMW) (P<0.05), highly significantly associated with hatch weight (HW), BW14, 21, 35, 42, 49, 90 and body length (BL), dressed weight (DW), eviscerated weight with giblet (EWG), wing weight (WW), breast muscle weight (BMW) and head and neck weight (HNW) (P<0.01). Meanwhile, A allele (with 'CTAACCTG') was positive for chicken growth as individuals with AA genotype had the highest value of all traits. Analysis on cGhrelin mRNA level revealed that it differed significantly among individuals with three genotypes (P<0.05). Individuals with AB genotype had the highest mRNA level, whereas that of AA had the lowest one. It was concluded that this 8 bp indel of cGHRL gene was significantly associated with most body weight and body composition traits, and negative effect of endogenous Ghrelin on chicken growth were indicated by this study.  相似文献   

基于斜率比法评定断奶仔猪对不同铜源的相对生物学利用率     

晏家友  罗宗刚  李书伟  汤文杰  邝声耀 《动物营养学报》2023,(1):148-156

本试验旨在研究不同铜源（硫酸铜、碱式氯化铜和柠檬酸铜）和铜添加水平（20和30 mg/kg）对断奶仔猪生长性能、血清铜含量与含铜酶活性以及组织铜含量的影响,探讨断奶仔猪对不同铜源的相对生物学利用率。试验采用3×2双因子随机区组设计,选取平均体重为（8.98±0.48） kg的杜×长×大三元杂交断奶仔猪112头,随机分为7组,每组4个重复,每个重复4头猪。对照组饲喂玉米-豆粕型基础饲粮（铜含量为7.80 mg/kg）,各试验组分别在基础饲粮中添加20或30 mg/kg硫酸铜、碱式氯化铜或柠檬酸铜（均以铜含量计）。试验预试期3 d,正试期28 d。结果表明：1）试验组与对照组断奶仔猪平均日增重、平均日采食量和料重比均无显著差异（P>0.05）。2）饲粮铜源和铜添加水平对断奶仔猪血清铜含量具有显著影响（P<0.05）,但对血清铜蓝蛋白和铜锌超氧化物歧化酶活性均无显著影响（P>0.05）。3）饲粮铜源和铜添加水平对断奶仔猪肝脏铜含量具有显著影响（P<0.05）,但对心脏、肾脏、胰脏和跖骨铜含量均无显著影响（P>0.05）。4）根据多元线性回归斜率比法计算,以血清铜...  相似文献   

四川省凉山黑绵羊疫病防控现状及对策     

李英林  吉色曲伍  孙艳  李呷比  惹及拉呷  拉马甲甲  罗旭  李维  周可磊  周文茂 《中国动物检疫》2023,40(2):62-65

凉山黑绵羊是我国十大优异畜禽遗传资源之一。通过问卷调查、实地调研和查阅资料等方式,发现近年来凉山黑绵羊产业得到了前所未有的发展,但疫病防控也面临着羊病流行情况复杂、免疫效果不佳、疫病监测预警能力弱等诸多短板,由此提出提高疫病综合防控能力和养殖户疫病防控意识,优化科学合理的免疫程序,加强监测预警能力建设,科学防治寄生虫病等防控策略,旨在提升凉山黑绵羊产业疫病防控能力,助力乡村振兴,保障国家核心畜禽种源安全。  相似文献   

网络驱动的协同设计三维几何模型共享技术     

罗天洪  杜子学  李军 《农业机械学报》2007,38(9):138-142

为解决网络驱动的协同设计三维几何模型共享技术的实现问题,提出一种基于特征流的混合式3D几何模型共享机制,提出了基于多粒度的产品几何模型数据结构描述方法和面向模型局部操作的"游历-查询"特征的历史记录来重构模型的特征建模方法。建立了网络驱动的、基于CORBA/JAVA中间插件的3D几何模型特征信息同步传输和共享平台,CORBA/JAVA平台支持多用户、分布数据管理、消息传输、网络通讯以及可扩展性,通过该平台实现了三维模型的实时传输和共享。  相似文献   

红酵母的性质及其应用研究   总被引：10，自引：0，他引：10  

李臣  阮榕生  林向阳  陈卫江  罗爱香  陈金海 《农产品加工．学刊》2006,(5):20-22,25

红酵母是酵母菌的一个属,腐生,分布广泛,在医学、食品、化工和农业等方面都有一定的应用,但目前并没有引起人们足够的重视。综述了红酵母的种属特性、理化特性、培养及其应用。  相似文献   

植物新品种保护在企业科技进步中的作用     

何勇  罗敏 《中国种业》2007,(12):16-17

种子企业要在种子竞争中立于不败之地，就必须积极加快科研开发的步伐，全面启动品种创新，培育出更有竞争力的农作物新品种，才能使企业步入健康发展的轨道。同时对获得的植物新品种还应该认真加强保护，这样既保障了企业的应有权益，也激励了农业技术持续创新，规范了市场经济秩序，从而发挥知识产权为企业带来的经济效益，促使企业的科技进步。  相似文献   

Suitability of Bi-linear Restoring Force Model in Earthquake Response Analysis     

Huang Zongming  Luo Zhijian 《保鲜与加工》1996,(2):110-114

Based on the data of dynamic tests of small single-storey steel frames,the suitability of simplified hi-linear restoring force model in earthquake response analysis is discussed, and the Maximum displacement response MD, the sum of squares of the displacements in the whole vibrating history SSD, and the total dissipated energy EI are considered respectively as equivalent object.  相似文献   

强度参数统计特性对边坡稳定可靠性的影响     

桂勇  罗嗣海  邓通发 《保鲜与加工》2015,(4):67-76

由于对边坡强度参数统计特性考虑不全面,使得边坡可靠性指标计算值偏小,对应的失效概率偏大,常常超过10%,设计中难以采纳,造成地基可靠度规范的制定和执行进展缓慢。以某高速公路全风化花岗岩土质高边坡为工程背景,在分析土质参数统计特性的基础上,采用极限平衡理论和蒙特卡罗模拟法,系统地分析了土质强度参数的均值不定性、变异性、相关性、区间特性和空间变化性等对边坡稳定可靠性的影响。结果表明：土性参数的各种统计特性对边坡稳定可靠性均具有不同程度的影响。可靠性指标计算值随抽样距离的减小而增加,随c或φ均值的增大而增加,随c和φ的变异系数的增大而减小,随c和φ相关系数绝对值的增大而增加,考虑区间性后可靠性指标计算值也明显变大。即变异性对边坡稳定不利,而相关性、区间性和空间变化性对边坡稳定有利。因此,准确而全面地考虑土质参数的统计特性,尤其是在参数变异性和相关性的基础上加以考虑土性参数空间变化性和区间性会更加符合工程实际,且计算结果趋于安全,有利于地基可靠度规范的推广运用和边坡工程的安全评价。  相似文献