Pasteurella multocida Heddleston serovars 1 and 14 express different lipopolysaccharide structures but share the same lipopolysaccharide biosynthesis outer core locus     

Harper M  St Michael F  John M  Vinogradov E  Adler B  Boyce JD  Cox AD 《Veterinary microbiology》2011,150(3-4):289-296

Pasteurella multocida strains are classified using the Heddleston lipopolysaccharide (LPS) serotyping scheme into 16 serovars. Understanding the structural and genetic basis for this LPS typing scheme is important because protection against infections caused by P. multocida is generally considered to be serovar specific. Here we show that the serovar 14 type strain P2225 and the serovar 1 strains X73 and VP161 express similar LPS structures. However, the serovar 14 LPS lacks the terminal phosphocholine (PCho) residues present on the serovar 1 LPS and contains the 1,4-linked β-galactose but not the 1,6-linked β-galactose. Sequencing analysis of the LPS biosynthesis outer core loci of P2225 and the serovar 1 type strain X73 showed that they were nearly identical. However, the phosphocholine biosynthesis gene, pcgA of P2225 contained a 19bp nucleotide deletion. Complementation of P2225 with an intact pcgA resulted in an LPS structure identical to that expressed by serovar 1 strain VP161 and highly similar to that expressed by strain X73, with a 1,6-linked β-galactose and both terminal PCho residues. This study has shown unequivocally that strains belonging to serovar 1 and 14 share a common LPS outer core locus and that minor changes within this locus can dramatically alter the LPS structure expressed on the surface of P. multocida, and thus has implications into our understanding of the potential to generate cross-protective vaccines.  相似文献   

Encounter with saturn: voyager 1 imaging science results     

Smith BA  Soderblom L  Beebe R  Boyce J  Briggs G  Bunker A  Collins SA  Hansen CJ  Johnson TV  Mitchell JL  Terrile RJ  Carr M  Cook AF  Cuzzi J  Pollack JB  Danielson GE  Ingersoll A  Davies ME  Hunt GE  Masursky H  Shoemaker E  Morrison D  Owen T  Sagan C  Veverka J  Strom R  Suomi VE 《Science (New York, N.Y.)》1981,212(4491):163-191

As Voyager 1 flew through the Saturn system it returned photographs revealing many new and surprising characteristics of this complicated community of bodies. Saturn's atmosphere has numerous, low-contrast, discrete cloud features and a pattern of circulation significantly different from that of Jupiter. Titan is shrouded in a haze layer that varies in thickness and appearance. Among the icy satellites there is considerable variety in density, albedo, and surface morphology and substantial evidence for endogenic surface modification. Trends in density and crater characteristics are quite unlike those of the Galilean satellites. Small inner satellites, three of which were discovered in Voyager images, interact gravitationally with one another and with the ring particles in ways not observed elsewhere in the solar system. Saturn's broad A, B, and C rings contain hundreds of "ringlets," and in the densest portion of the B ring there are numerous nonaxisymmetric features. The narrow F ring has three components which, in at least one instance, are kinked and crisscrossed. Two rings are observed beyond the F ring, and material is seen between the C ring and the planet.  相似文献   

Estimating the mass of asteroid 253 mathilde from tracking data during the NEAR flyby   总被引：1，自引：0，他引：1  

DK Yeomans  J Barriot  DW Dunham  RW Farquhar  JD Giorgini  CE Helfrich  AS Konopliv  JV McAdams  JK Miller  WM Owen  DJ Scheeres  SP Synnott  BG Williams 《Science (New York, N.Y.)》1997,278(5346):2106-2109

The terminal navigation of the Near Earth Asteroid Rendezvous (NEAR) spacecraft during its close flyby of asteroid 253 Mathilde involved coordinated efforts to determine the heliocentric orbits of the spacecraft and Mathilde and then to determine the relative trajectory of the spacecraft with respect to Mathilde. The gravitational perturbation of Mathilde on the passing spacecraft was apparent in the spacecraft tracking data. As a result of the accurate targeting achieved, these data could be used to determine Mathilde's mass as 1.033 (+/- 0.044) x 10(20) grams. Coupled with a volume estimate provided by the NEAR imaging team, this mass suggests a low bulk density for Mathilde of 1.3 grams per cubic centimeter.  相似文献   

Evaluation of phenotypic markers in full genome sequences of avian influenza isolates from California     

Eva Mertens  Vivien G. Dugan  Timothy B. Stockwell  LeAnn L. Lindsay  Magdalena Plancarte  Walter M. Boyce 《Comparative immunology, microbiology and infectious diseases》2013

We evaluated phenotypic markers in full-genome sequences of avian influenza isolates to identify avian strains with increased potential for transmission and pathogenicity in mammals. Of 149 markers examined, 67 were positive in the consensus sequences from 206 avian isolates. Analysis of deep sequencing data in a subset of 24 isolates revealed that 344 subpopulations occurred at marker positions. Markers in subpopulations were significantly more likely to be negative (258/344) than positive (86/344), but nearly all of the marker-positive subpopulations (78/86) were associated with marker-negative consensus sequences. Our analysis revealed significant variation in important markers among avian isolates, and showed that consensus sequences do not fully convey an isolate's potential for increased transmissibility and pathogenicity in mammals.  相似文献   

NONINVASIVE ESTIMATION OF CENTRAL VENOUS PRESSURE IN ANESTHETIZED DOGS BY MEASUREMENT OF HEPATIC VENOUS BLOOD FLOW VELOCITY AND ABDOMINAL VENOUS DIAMETER     

NATHAN C. NELSON  WM TOD DROST  PHILLIP LERCHE  JOHN D. BONAGURA 《Veterinary radiology & ultrasound》2010,51(3):313-323

Determination of central venous pressure (CVP) is relevant to patients with right heart disease, hypovolemia, and following intravenous fluid therapy. We hypothesized that changes in CVP in dogs could be predicted by measurements of hepatic vein diameter, caudal vena cava (CVC) diameter, and hepatic venous flow velocities. Nine healthy American Foxhounds were anesthetized. Following baseline recordings, intravenous fluids were administered to increase CVP. Volume administration created treatment periods with CVP ranges of 5, 10, 15, 20, and 25 mm Hg. Flow velocities in the right medial hepatic vein were recorded using pulsed wave Doppler ultrasound. Hepatic vein, CVC, and aorta diameters were determined with B‐mode ultrasound. Variables were compared across the treatment periods by ANOVA for repeated measures. Relationships between CVP, Doppler, and B‐mode variables were evaluated using Spearman's rank correlations, multiple linear regression, and repeated measures linear regression. The a‐, S‐ and v‐wave velocities were augmented significantly with volume loading. The best part (semipartial) correlation coefficients predicting increasing CVP were identified with v‐wave velocity (0.823), S‐wave velocity (?0.800), CVC diameter (0.855), and hepatic vein diameter (0.815). Multiple linear regression indicated that CVP in this study could be predicted best by a combination of CVC and hepatic vein diameter and the v‐wave velocity (r=0.928). Ultrasound imaging identified gallbladder and pancreatic edema consistently, likely related to acute volume loading. These findings may be applicable in the assessment of volume status, dogs with right heart disease, and during serial monitoring of dogs receiving fluid or diuretic therapy.  相似文献   

Bragg diffraction from crystallized ion plasmas     

WM Itano  JJ Bollinger  JN Tan  B Jelenkovic  X Huang  DJ Wineland 《Science (New York, N.Y.)》1998,279(5351):686-689

Single crystals of a one-component plasma were observed by optical Bragg diffraction. The plasmas contained 10(5) to 10(6) single-positive beryllium-9 ions (9Be+) at particle densities of 10(8) to 10(9) per cubic centimeter. In approximately spherical plasmas, single body-centered cubic (bcc) crystals or, in some cases, two or more bcc crystals having fixed orientations with respect to each other were observed. In some oblate plasmas, a mixture of bcc and face-centered cubic ordering was seen. Knowledge of the properties of one-component plasma crystals is required for models of white dwarfs and neutron stars, which are believed to contain matter in that form.  相似文献   

Salt Hypertrophy in Succulent Dune Plants     

Boyce SG 《Science (New York, N.Y.)》1951,114(2969):544-545

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Familial renal amyloidosis in Abyssinian cats   总被引：1，自引：0，他引：1  

J T Boyce  S P DiBartola  D J Chew  P W Gasper 《Veterinary pathology》1984,21(1):33-38

Medullary and glomerular amyloidosis, papillary necrosis, and secondary interstitial disease were diagnosed in eight related adult Abyssinian cats from two catteries. The lesions were similar to those in two unrelated mongrel cats with renal amyloidosis. Ultrastructurally, the patterns of amyloid deposition were as described in other species, although medullary deposition predominated. Potassium permanganate oxidation blocked Congo red staining of the deposits suggesting that they contained amyloid A protein (secondary amyloid). The disease may be a model of familial secondary amyloidosis and offers an opportunity to study the pathogenesis of both amyloid deposition and papillary necrosis. The histochemical characteristics of feline renal amyloid require careful attention to technique. Section thickness affects Congo red affinity and both dichroism as well as birefringence should be considered when interpreting staining reactions. Thioflavine-T may be the preferred stain for identification of small deposits of amyloid. Variation in section thickness markedly affected the degree of potassium permanganate oxidation.  相似文献   

Care and use of animals. Probing four views of animal use     

J R Boyce 《Journal of the American Veterinary Medical Association》1990,196(9):1368-1369

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Sebekiosis in captive alligator hatchlings     

W Boyce  P Cardeilhac  T Lane  C Buergelt  M King 《Journal of the American Veterinary Medical Association》1984,185(11):1419-1420

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