Prevalence of methicillin-resistant Staphylococcus aureus (MRSA) in broilers and workers at ‘pluck shops’ in Trinidad     

Stewart-Johnson  Alva  Dziva  Francis  Abdela  Woubit  Rahaman  Saed  Adesiyun  Abiodun 《Tropical animal health and production》2019,51(2):369-372

Tropical Animal Health and Production - Livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) is a cause of zoonotic infections in many countries. People with occupational...  相似文献   

Nitrogen best management practice for citrus trees: I. Fruit yield,quality, and leaf nutritional status     

A.K. Alva  S. Paramasivam  T.A. Obreza  A.W. Schumann 《Scientia Horticulturae》2006

Elevated levels of nitrate-nitrogen (NO₃-N) in the surficial aquifer above the drinking water quality standard, i.e. maximum contaminant limit (MCL; 10 mg L⁻¹), have been reported in some part of central Florida citrus production regions. Soils in this region are very sandy (sand content >95%), hence are vulnerable to leaching of soluble nutrients and chemicals below the rooting depth of the trees. The objective of this research was to develop N and irrigation best management practices for citrus in sandy soils to maintain optimal crop yield and quality, and to minimize potential leaching of nitrate below the root zone. Six years of field experiment was conducted in a high productive (mean fruit yield > 80 Mg ha⁻¹yr⁻¹) >20-year-old ‘Hamlin’ orange trees [Citrus sinensis (L.) Osbeck] on ‘Cleopatra mandarin’ (Citrus reticulata Blanco) rootstock grown on a well drained Tavares fine sand (hyperthermic, uncoated, Typic Quartzipsamments) in Highland county, FL. Nitrogen rates ranged from 112 to 280 kg ha⁻¹ yr⁻¹ applied as fertigation (FRT), water soluble granular (WSG), 50:50 mix of FRT and WSG, and controlled-release fertilizer (CRF). Tensiometers were used to monitor the soil water content as a basis to schedule optimal irrigation. Fruit yield response over the entire range of N rates was greater for the FRT and WSG sources as compared to that for the WSG + FRT or CRF sources. Using the regression analysis of the fruit yield in relation to N rate, the optimum N rate appeared to be at 260 kg ha⁻¹ yr⁻¹. Based on fruit production response in this study, the N requirement for production of 1 Mg of fruit varied from 2.2 to 2.6 kg across four N sources. This study demonstrated an increased N uptake efficiency, as a result of best management of N and irrigation applications. The optimal N and K concentration in the 4–6-month-old spring flush leaves were 26–30, and 15–18 g kg⁻¹, respectively. However, fruit yield response showed no significant relationship with concentrations of P in the 4–6-month-old spring flush leaves over a range of 0.8–2.4 g kg⁻¹. The results of fate and transport of N in soil and in soil solution with application of different rates and sources of N, and components of citrus tree N budget, are reported in a companion paper.  相似文献   

Genomic diversity among Danish field strains of Mycoplasma hyosynoviae assessed by amplified fragment length polymorphism analysis     

Kokotovic B  Friis NF  Nielsen EO  Ahrens P 《Veterinary microbiology》2002,85(3):221-231

Genomic diversity among strains of Mycoplasma hyosynoviae isolated in Denmark was assessed by using amplified fragment length polymorphism (AFLP) analysis. Ninety-six strains, obtained from different specimens and geographical locations during 30 years and the type strain of M. hyosynoviae S16(T) were concurrently examined for variance in BglII-MfeI and EcoRI-Csp6I-A AFLP markers. A total of 56 different genomic fingerprints having an overall similarity between 77 and 96% were detected. No correlation between AFLP variability and period of isolation or anatomical site of isolation could be demonstrated. An examination of the clonal appearance of M. hyosynoviae isolates recovered from seven herds affected with arthritis revealed presence of several genotypically distinct variants of the organism in a single herd, indicating that different strains may simultaneously be involved in an outbreak of the disease.  相似文献   

Comparison of efficacy and safety of paste formulations of firocoxib and phenylbutazone in horses with naturally occurring osteoarthritis   总被引：1，自引：0，他引：1  

Doucet MY  Bertone AL  Hendrickson D  Hughes F  Macallister C  McClure S  Reinemeyer C  Rossier Y  Sifferman R  Vrins AA  White G  Kunkle B  Alva R  Romano D  Hanson PD 《Journal of the American Veterinary Medical Association》2008,232(1):91-97

OBJECTIVE: To compare efficacy and safety of paste formulations of firocoxib and phenylbutazone in horses with naturally occurring osteoarthritis. DESIGN: Randomized controlled clinical trial. ANIMALS: 253 client-owned horses with naturally occurring osteoarthritis. PROCEDURES: Horses were treated with firocoxib (0.1 mg/kg [0.045 mg/lb], PO, q 24 h) or phenylbutazone (4.4 mg/kg [2 mg/lb], PO, q 24 h) for 14 days. Physical examinations and lameness evaluations were performed prior to treatment and after 7 and 14 days. Clinical improvement was defined as a reduction of at least 1 lameness grade or a combined reduction of at least 3 points in scores for pain during manipulation or palpation, joint swelling, joint circumference, and range of motion. RESULTS: Proportion of horses clinically improved on day 14 for the firocoxib group (104/123 [84.6%]) was not significantly different from the proportion for the phenylbutazone group (103/119 [86.6%]). Proportion of horses that were improved on day 14 was significantly greater for horses treated with firocoxib than for horses treated with phenylbutazone with regard to score for pain on manipulation or palpation (P = 0.028), joint circumference score (P = 0.026), and range of motion score (P = 0.012), but not for overall lameness score or joint swelling score. No direct treatment-related adverse effects were detected during the study. CONCLUSIONS AND CLINICAL RELEVANCE: Results suggested that overall clinical efficacy of a paste formulation of firocoxib in horses with naturally occurring osteoarthritis was comparable to efficacy of a paste formulation of phenylbutazone.  相似文献   

Use of the Urease Inhibitor N-(n-BUTYL)-Thiophosphoric Triamide Decreased Nitrogen Leaching from Urea in a Fine Sandy Soil     

Prakash  O.  Alva  A. K.  Paramasivam  S. 《Water, air, and soil pollution》1999,116(3-4):587-595

Water, Air, &; Soil Pollution - Application of soluble forms of nitrogen (N) fertilizers to citrus trees in sandy soils of central Florida may cause leaching of NO 3 ? below the rooting...  相似文献   

Distribution of rainfall and soil moisture content in the soil profile under citrus tree canopy and at the dripline   总被引：2，自引：0，他引：2  

A. K. Alva  O. Prakash  Ali Fares  Arthur G. Hornsby 《Irrigation Science》1999,18(3):109-115

The plant canopy intercepts rain and thus can alter the distribution of water under the canopy as compared to that along the dripline. The effects of a citrus (Citrus sinensis L. Osbeck) tree (25-year-old, Valencia orange) canopy on the distribution of rainfall and soil moisture content within the soil profile either along the dripline (D) or under the canopy near the trunk (inner side; I), and midway between I and Dripline (M) were evaluated, on the east and west sides of trees planted along north-south rows. Results of eleven storm events in 1995 (mean of east and west sides) revealed that the amounts of precipitation at the D, M, and I positions were 97–140, 47–94, and 52–79% of the incident rainfall, respectively. Thus, canopy interception of incident rainfall was quite appreciable. The soil moisture content was greater along the dripline compared to that at the M and I positions, particularly in the deeper (≥60 cm) soil profile. The water flux was significantly greater at the dripline than under the canopy indicating a greater leaching potential of soil-applied fertilizers and other chemicals when placed along the dripline. A substantial reduction in the rainfall and water flux under the canopy as a result of canopy interception suggests that application of fertilizer and chemicals under the canopy could minimize leaching losses. Received: 10 November 1997  相似文献   

Fruit nutrient accumulation of four orange varieties during fruit development 1     

S. Paramasivam  A. K. Alva  K. H. Hostler  G. W. Easterwood  J. S. Southwell 《Journal of plant nutrition》2013,36(3):313-327

Evaluation of nutrient accumulation trends in fruit during fruit development and nutrient status in the leaves are important components defining nutrient requirements. Such nutrient demand should be met by nutrient supply in order to develop optimal rate and timing of fertilizer application. In a 3‐year study the citrus (orange) varieties, ‘Valencia’, ‘Parson Brown’, ‘Hamlin’, and ‘Sunburst’ were fertilized with either 168, 224, or 280 kg N ha^‐1 yr^‐1 as a broadcast application of N:P:K dry soluble granular fertilizer. These rates did not significantly affect the fruit nutrient status, juice quality, or nutrients concentrations in the spring flush. The concentrations of nitrogen (N), phosphorus (P), potassium (K), calcium (Ca), and magnesium (Mg) in the fruit decreased with their enlargement during June through November. However, micronutrient concentrations in the fruits increased from June through August or September and then decreased. Fruit dry weight was greatest in ‘Valencia’ followed by ‘Hamlin’, ‘Parson Brown’, and ‘Sunburst’ varieties. Soluble solids concentration (SSC) were higher in ‘Valencia’ and ‘Sunburst’ fruit than in the other two varieties. Other juice quality parameters were not significantly different among the varieties. This study indicated non‐significant influence of fertilizer rates on concentrations of various mineral elements in 6‐month‐old spring flush with most of those concentrations within the optimal ranges.  相似文献   

Potential for Biocontrol of Monosporascus Root Rot/vine Decline Under Greenhouse Conditions using Hypovirulent Isolates of Monosporascus cannonballus   总被引：1，自引：0，他引：1  

Jeffrey S. Batten  Karen-Beth G. Scholthof  Branko R. Lovic  Marvin E. Miller  Raymond D. Martyn 《European journal of plant pathology / European Foundation for Plant Pathology》2000,106(7):639-649

Monosporascus root rot/vine decline (MRR/VD) causes root necrosis and severe stunting of muskmelon and watermelon plants in several countries around the world. MRR/VD is caused by the soilborne ascomycete fungus, Monosporascus cannonballus. Currently, there are few options available for control of MRR/VD. This research describes experiments to test the possibility of using naturally occurring M. cannonballus isolates containing double-stranded RNA (dsRNA) for the biological control of MRR/VD. These isolates often develop a degenerate phenotype characterized by slow growth and reduced ascospore production. In addition, these degenerate isolates are hypovirulent on muskmelon. Plants co-inoculated with a hypovirulent, dsRNA⁺ isolate (Tx93-449⁺) and a virulent, dsRNA^- isolate (Az90-33^-) at an inoculum ratio of 10:1 (hypovirulent:virulent) were indistinguishable from the uninoculated plants in greenhouse pathogenicity trials. In vitro infection assays using fluorescence microscopy on aniline-stained muskmelon roots suggested that although the hypovirulent dsRNA⁺ isolate Tx93-449⁺ penetrated and partially colonized roots of the seedlings, it was not as efficient in colonizing the roots as the virulent, dsRNA^- isolate Az90-33^-. While more extensive experiments are needed, these data suggest that hypovirulent dsRNA⁺ isolates of M. cannonballus have potential for development as biological control agents to reduce disease pressure associated with MRR/VD.  相似文献   

Effects of soil-cation composition on reactions of four herbicides in a candler fine sand     

A. K. Alva  Megh Singh 《Water, air, and soil pollution》1990,52(1-2):175-182

Sorption, persistence and transport of herbicides in soils depend on the relative saturation of soils with cations from various soil amendments. Current research was conducted to study the effect of preequilibration of a Candler fine sand (Hyperthermic uncoated typic Quartzipsamments; 0–30 cm depth) with AlCl₃, CaCl₂, CuCl₂, FeCl₃, or KCl salt solutions on sorption in bromacil, simazine, norflurazon, and diuron herbicides commonly used in Florida citrus groves. Preequilibration of the soil with either FeCl₃, or AlCl₃ significantly decreased the sorption and therefore increased internal leaching potential, of all four herbicides as compared to their sorption in untreated soil. This decrease in sorption was much greater for bromacil and simazine (24 to 35%) than for norflurazon and diuron (7 to 8%). The desorption of bromacil and diuron in 1M NH₄OAc was also significantly lower in soils preequilibrated with FeCl₃ or AlCl₃ than the untreated soil. However, the reverse was true in the case of simazine and norflurazon. Preequilibration of the soil with CuCl₂, KCl, and CaCl₂ resulted in a significant decrease in sorption of norflurazon, diuron, and simazine but did not affect bromacil sorption. Accordingly, the species of adsorbed cation had varying effects on the sorption/desorption of each of the herbicides and varied their leaching potential.  相似文献   

Study on the effects of rearing density, temperature and salinity on hatching performance of the European sea bass, Dicentrarchus labrax (Linnaeus, 1758)     

Alexis J. Conides  Branko Glamuzina 《Aquaculture International》2002,9(3):217-224

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