Apparent ELISA detection times for albuterol after administration with the torpex equine inhaler device     

Dirikolu L  Mollett BA  Troppmann A  Woods WE  Bratton C  Cashman CP  Schroedter D  Mayer B  Lehner AF  Karpiesiuk W  Hughes C  Boyles J  Harkins JD  Tobin T 《Veterinary therapeutics : research in applied veterinary medicine》2002,3(3):297-307

Single doses of one, three, and six actuations (120 micro g albuterol/actuation) and multiple daily doses (six actuations per dose four times daily) for 5 days of aerosol albuterol sulfate were sequentially administered to each of six horses using an equine inhaler device (Torpex, 3M Animal Care Products, St. Paul, MN [corrected] and Boehringer Ingleheim Vetmedica, Inc., St. Joseph, MO [corrected]). A 2-week washout period was allowed between each dose. ELISA testing revealed no evidence of albuterol in urine at 24 hours after any single-dose administration. Results indicated that 48 hours or longer should be allowed for albuterol to be cleared from urine after single doses. When given at the maximum recommended rate of six actuations per dose four times a day for 5 days, urine samples tested by ELISA showed no evidence of albuterol at 48 hours after the final dose. Testing of nasal swabs by ELISA demonstrated the presence of albuterol for 8 hours after each single dose, and some horses might have detectable levels of albuterol in nasal swabs for several days following administration of multiple doses. As a guideline for withdrawal time, 72 hours or longer should be allowed after administration of aerosol albuterol sulfate to horses before participation in equestrian competitions that are regulated for detection of certain performance-enhancing substances. However, these recommendations were based on a small sample of horses and the specific ELISA test used and interpreted as described. Factors specific to individual horses may influence these detection times.  相似文献   

Inhibition of appressorial adhesion of Pyricularia oryzae to barley leaves by fungicides     

Satoru Inoue  Toshiro Kato  Victor W. L. Jordan  Keith J. Brent 《Pest management science》1987,19(2):145-152

The effects of rice blast fungicides known to inhibit melanisation or penetration on the adhesion of Pyricularia oryzae to the leaf surface of barley were investigated. Adhering appressoria were counted after shaking inoculated leaves vigorously with water. On untreated leaves, appressoria began to adhere at the time when appressorial melanisation was initiated. The number of adhering appressoria then increased gradually, and most were melanised. On chlobenthiazone-treated leaves, appressoria were not melanised and were easily detached from the surface. Similar results were obtained with tricyclazole. However, after treatment with tetrachlorophthalide or pentachlorobenzyl alcohol, which are also known to prevent penetration of P. oryzae, melanised appressoria still remained after shaking in water. It is suggested that appressorial melanisation in P. oryzae is involved in appressorial adhesion. The antipenetrant action of the melanin biosynthesis inhibitors chlobenthiazone and tricyclazole may be ascribed to the lack of adhesive intensity necessary to support the mechanical shearing of the cuticle of rice plants.  相似文献   

Comparison of antimicrobial resistance in generic Escherichia coli and Salmonella spp. cultured from identical fecal samples in finishing swine          下载免费PDF全文

Csaba Varga  Andrijana Raji&#x;  Margaret E. McFall  Richard J. Reid-Smith  Anne E. Deckert  David L. Pearl  Brent P. Avery  Sylvia L. Checkley    Scott A. McEwen 《Canadian journal of veterinary research》2008,72(2):181-187

The objectives of this study were to investigate the associations between antimicrobial resistance patterns in generic Escherichia coli and Salmonella spp. isolates recovered from identical pen pooled fecal samples, and to evaluate potential clustering of multiple isolates of these organisms within identical fecal samples. Up to 5 generic E. coli (n = 922 isolates) and Salmonella spp. (n = 922 isolates) isolates were obtained from each of 188 pen pooled fecal samples that had been collected from 45 finishing swine farms in Alberta in 2000, and tested for susceptibility to 15 antimicrobials. No isolates of either organism were resistant to 3rd generation cephalosporins or fluoroquinolones, which in Canada are considered antimicrobials of very high importance to human health. Approximately twice as many generic E. coli isolates as Salmonella spp. isolates were resistant to at least 1 antimicrobial. In addition, E. coli isolates showed more multidrug-resistance patterns. No significant association was observed between the resistance phenotypes of Salmonella spp. and E. coli at the fecal sample level. More clustering at the sample level was observed for proportions of antimicrobial resistance (AMR) in Salmonella spp. isolates than E. coli indicating that in future studies it might be sufficient to test fewer than 5 Salmonella spp. isolates per sample.  相似文献   

Wheat diseases on the prairies: A Canadian story     

Reem Aboukhaddour  Thomas Fetch  Brent D. McCallum  Michael W. Harding  Brian L. Beres  Robert J. Graf 《Plant pathology》2020,69(3):418-432

Canada is one of the largest wheat producers in the world, and wheat is grown over an area spanning most of the southern latitudes, with the prairie region (provinces of Saskatchewan, Alberta, and Manitoba) being the main producer. Several pathogens and pests attack wheat, but at present fusarium head blight (FHB), stripe rust, and leaf spots are the most damaging diseases to wheat production in Canada. Historically, smuts, stem rust, and leaf rust caused major crop losses in Canada and can still pose serious threat if management practices are relaxed. Cropping practices used by Canadian farmers to grow and harvest wheat over the last century have influenced disease development and pathogen biology, affecting the severity, incidence, and prevalence of crop diseases over time. Changes such as reduced tillage coincide with emergence of residue-borne diseases, such as FHB and leaf spots, while the deployment of resistant cultivars and increased fungicide use has resulted in the reduction of common bunt, stem, and leaf rust. This review will discuss the influence of long-term cropping practices, alone or in combination, on the biology, emergence, and prevalence of wheat diseases in Canada over the last century.  相似文献   

Achieving sustainable aquaculture: Historical and current perspectives and future needs and challenges     

Claude E. Boyd  Louis R. D'Abramo  Brent D. Glencross  David C. Huyben  Lorenzo M. Juarez  George S. Lockwood  Aaron A. McNevin  Albert G. J. Tacon  Fabrice Teletchea  Joseph R. Tomasso Jr  Craig S. Tucker  Wagner C. Valenti 《Journal of the World Aquaculture Society》2020,51(3):578-633

Important operational changes that have gradually been assimilated and new approaches that are developing as part of the movement toward sustainable intensive aquaculture production systems are presented via historical, current, and future perspectives. Improved environmental and economic sustainability based on increased efficiency of production continues to be realized. As a result, aquaculture continues to reduce its carbon footprint through reduced greenhouse gas emissions. Reduced use of freshwater and land resources per unit of production, improved feed management practices as well as increased knowledge of nutrient requirements, effective feed ingredients and additives, domestication of species, and new farming practices are now being applied or evaluated. Successful expansion into culture of marine species, both off and on shore, offers the potential of substantial increases in sustainable intensive aquaculture production combined with integrative efforts to increase efficiency will principally contribute to satisfying the increasing global demand for protein and food security needs.  相似文献   

Preparation and characterization of the foam-stabilizing properties of cellulose-ethyl cellulose complexes for use in foods     

Murray BS  Durga K  de Groot PW  Kakoulli A  Stoyanov SD 《Journal of agricultural and food chemistry》2011,59(24):13277-13288

Surface active cellulose particles have been prepared for use as foam stabilizing agents in foods. Various sources of cellulose were broken down by combinations of milling, acid dissolution and treatment with cellulase. The most efficient and simple method was hammer and freezer milling of dry crystalline α-cellulose (Tencel). The resultant Tencel particles were made partially hydrophobic through precipitation of ethyl cellulose (EC) onto them in acetone-water dispersions. The optimum ratio of EC to cellulose and the optimum solids concentration (C(x)) at which to form the complexes were 1:1 and C(x) ≈ 1 wt %, respectively. Complexes combined at low concentrations (e.g., C(x) ≈ 0.1 wt %) with caseins or whey proteins gave significant improvements in stability of foams and bubbles to coalescence and disproportionation compared to either component alone. As such, the complexes could be a useful ingredient in improving the quality of various food foams.  相似文献   

Particle-stabilizing effects of flavonoids at the oil-water interface     

Luo Z  Murray BS  Yusoff A  Morgan MR  Povey MJ  Day AJ 《Journal of agricultural and food chemistry》2011,59(6):2636-2645

It has been shown that some common food flavonoids can act as excellent stabilizers of oil-in-water emulsions through their adsorption as water-insoluble particles to the surface of the oil droplets, i.e., Pickering emulsions are formed. Flavonoids covering a wide range of octanol-water partition coefficients (P) were screened for emulsification behavior by low shear mixing of flavonoid+n-tetradecane in a vortex mixer. Most flavonoids with very high or very low P values were not good emulsifiers, although there were exceptions, such as tiliroside, which is very insoluble in water. When a high shear jet homogenizer was used with 20 vol% oil in the presence of 1 mM tiliroside, rutin, or naringin, much finer emulsions were produced: the average droplet sizes (d32) were 16, 6, and 5 μm, respectively. These results may be highly significant with respect to the delivery of such insoluble compounds to the gut, as well as their digestion and absorption.  相似文献   

Pesticide resistance and world food production: ed. by Gordon Conway. ISBN 0-950-7744-1-3 (144 pp; £3.60 inc. postage and packing). London: Imperial College Centre for Environmental Technology     

K.J. Brent 《Crop Protection》1984,3(1):129-130

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Analyses of rumen fluid from "sudden death", lactic acidotic and healthy cattle fed high concentrate ration.     

J R Wilson  E E Bartley  H D Anthony  B E Brent  D A Sapienza  T E Chapman  A D Dayton  R J Milleret  R A Frey  R M Meyer 《Journal of animal science》1975,41(5):1249-1255

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Forensic pathology of canadian bread wheat: the case of tan spot     

Lamari L  McCallum BD  Depauw RM 《Phytopathology》2005,95(2):144-152

ABSTRACT Pyrenophora tritici-repentis causes necrosis and chlorosis in its wheat host. Susceptibility to races 2 (necrosis) and 5 (chlorosis) of the pathogen is known to be mediated by Ptr ToxA and Ptr ToxB, respectively. Sensitivity to each toxin is controlled by a single dominant and independently inherited gene. We used sensitivity to Ptr ToxA and Ptr ToxB as two genetic markers to investigate the origin and the state of tan spot susceptibility in Canadian Western Red Spring (CWRS) wheat over a period of more than a century. Sensitivity to Ptr ToxA, the toxin produced by nearly all isolates of the pathogen collected in the past 20 years in western Canada, appears to have been present in the first major cultivar, Red Fife, grown massively in the late 1800s. Sensitivity then was transmitted unknowingly into Canadian wheat lines through extensive use of backcrossing to maintain the Marquis-Thatcher breadmaking quality. Sensitivity to Ptr ToxA, which nearly disappeared from cultivars grown in western Canada in the 1950s, was reintroduced in the 1960s and unintentionally bred into many of the present-day cultivars. Sensitivity to Ptr ToxB, a toxin rarely found in isolates from western Canada, appeared with the release of Thatcher in 1934 and was transferred to many cultivars through backcross programs. In spite of large areas planted to Ptr ToxAand Ptr ToxB-sensitive cultivars over decades, tan spot epidemics remained sporadic until the 1970s. The results of this study raise the problem of the narrowing genetic base of CWRS wheat lines and the potential for unanticipated threats from plant pathogens. The intercrossing of genetically diverse material in one Canadian wheat breeding program resulted in the release of several modern cultivars with resistance to tan spot. The absence of wild-type Ptr ToxB-producing isolates in western Canada remains unexplained, given that sensitivity to Ptr ToxB was present continuously in western Canadian cultivars grown on vast areas for more than 70 years.  相似文献