Ovine enzootic abortion (OEA): antibody response in vaccinated sheep compared to naturally infected sheep     

Borel N  Sachse K  Rassbach A  Bruckner L  Vretou E  Psarrou E  Pospischil A 《Veterinary research communications》2005,29(Z1):151-156

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A Low Oxygen Atmosphere during IVF Accelerates the Kinetic of Formation of In Vitro Produced Ovine Blastocysts     

GG Leoni  I Rosati  S Succu  L Bogliolo  D Bebbere  F Berlinguer  S Ledda  S Naitana 《Reproduction in domestic animals》2007,42(3):299-304

Among the factors that affect in vitro embryo development, oxygen atmosphere is considered to be of great influence. In this study, we evaluated the influence of two different oxygen atmospheres during in vitro fertilization (IVF) of ovine oocytes on their developmental capacity and quality assessed by cryotolerance. Cumulus oocyte complexes derived from ovaries of slaughtered sheep were matured in vitro and subsequently fertilized under low (5%) or high (20%) oxygen atmospheres, and cultured in SOF + aa + 0.4% BSA in 5% CO2 and 5% O2 up to blastocyst stage. The cleavage rates obtained in the fertilization system at 20% O2 were significantly higher than those obtained in the 5% O2 fertilization system (61.2% vs 50.8%; p < 0.01). The distribution of cleaved oocytes at 22, 26 and 40 h of culture intervals was not different in the low or high O2 atmosphere (31.4%, 26.4% and 42.1% vs 28.0%, 29.3% and 42.7% respectively). Blastocysts output on the 6th day post-fertilization (dpf) was significantly higher when oocytes were fertilized under 5% O2 concentration (63.04% in 5% O2 vs 47.36% in 20% O2), while on the 7th dpf the higher number of blastocysts was obtained in the 20% O2 system (35.10%.in 20% O2 vs 26.09% in 5% O2). After vitrification no differences were observed between low or high oxygen atmosphere in the viability rates of blastocysts obtained on day 6 (93.6% vs 96.5%), on day 7 (46.3% vs 41.7%) and on day 8 (11.1% vs 6.6%). After differential staining, no significant differences were observed in the total cell number and inner cell mass and trophoblastic cells ratio of blastocysts produced on 6 dpf (189.6 +/- 51.3 and 0.260 +/- 0.07 vs 223.3 +/- 45.6 and 0.277 +/- 0.09), on 7 dpf (168.3 +/- 25.1 and 0.316 +/- 0.06 vs 172.1 +/- 33,6 and 0.320 +/- 0.06) and on 8 dpf (121.2 +/- 23,8 and 0.302 +/- 0.03 vs 117.0 +/- 35.1 and 0.313 +/- 0.04) under low or high oxygen atmosphere respectively). In conclusion, our data suggest that low oxygen atmosphere during IVF affects positively the production of high quality ovine blastocysts.  相似文献   

Chlamydiosis in commercial ducks   总被引：1，自引：0，他引：1  

KE ARZEY  GG ARZEY  RL REECE 《Australian veterinary journal》1990,67(9):333-334

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The effectiveness of foot-and-mouth disease vaccines in Switzerland. II. Stability problems     

M Ackermann  H K Müller  L Bruckner  U Kihm 《Schweizer Archiv für Tierheilkunde》1989,131(7):389-395

One of the vaccines that were used in 1988 to immunize the Swiss national cattle population against foot-and mouth disease (FMD) was apparently not stable. Data, provided by the manufacturer, indicated a high initial antigenic content for serotype O. Protection experiments at the end of the vaccination campaign, however, indicated a substantial loss of serotype O antigen in the vaccine. Serological data, obtained during the campaign indicated that only 12% of the primovaccinated animals and 63% of previously vaccinated animals received an amount of FMD viral antigen sufficient to induce protective immunity. The primovaccinated animals were revaccinated in fall 1988 with a new batch provided by the same manufacturer. The new vaccine induced high titers of neutralizing antibodies in primo- and an anamnestic response in revaccinated cattle.  相似文献   

Serologic studies of rabies vaccination in dogs in Switzerland     

L Bruckner  M Ackermann  H K Müller  U Kihm 《Schweizer Archiv für Tierheilkunde》1986,128(4):195-200

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Molecular and immunodiagnostic studies of bovine neosporosis in Switzerland     

Gottstein B  Hentrich B  Wyss R  Thür B  Bruckner L  Müller N  Kaufmann H  Waldvogel A 《Schweizer Archiv für Tierheilkunde》1999,141(2):59-68

Cyst-forming coccidia may cause significant losses in livestock, primarily due to abortion, loss of young animals and neuromuscular diseases. Rather recently, Neospora caninum has been recognized as one of the major protozoal abortion-inducing parasites in cattle. The present study addressed the performance of different diagnostic tools (in vitro-cultivation; histology; immunohistochemistry; serology; PCR) suitable for the direct or indirect detection of N. caninum. By PCR, Neospora-DNA was detected in 24 brains (29%) from 83 bovine abortion, many of these brains were simultaneously characterized by histopathological findings typical for a protozoal, cerebral parasitosis. The diagnostic methods were furthermore assessed using samples of different tissues and body fluids from three experimentally Neospora-infected pregnant cows and their foetuses. The diaplacental passage of N. caninum to the foetus was successful in two of the three cases. In these two cases, PCR was positive for different foetal organs and, additionally, for the abomasal and amniotic fluid. The successfully infected cows developed anti-Neospora serum antibodies between 10 and 17 days post infection, foetuses remained serologically negative in all cases. The results obtained in the present study demonstrated the usefulness of PCR, complemented by serology, for the specific diagnosis of bovine neosporosis. Such tests may prove suitable to perform epidemiological investigations. Taken together, our data indicated that prenatal neosporosis may be an important cause of infectious bovine abortion in Switzerland.  相似文献   

Equine travellers to the Olympic Games in Hong Kong 2008: a review of worldwide challenges to equine health, with particular reference to vector-borne diseases     

Herholz C  Füssel AE  Timoney P  Schwermer H  Bruckner L  Leadon D 《Equine veterinary journal》2008,40(1):87-95

The past 10-20 years have seen exponential growth in the volume of trade in horses and equine germplasm; and the extent of global horse movements has increased significantly in the last 4 years. In preparing for the transport of elite Olympic horses to Hong Kong in 2008, it will be very important to be as fully informed as possible of the disease situation in both the exporting and importing country, import and re-entry requirements, as well as having a vaccination strategy to protect against particular diseases. In this context the review describes the equine vector-borne disease situation in Europe, Asia, Africa and South America and provides estimates of the number of horse movements between these countries, as well as information on import requirements and vaccination strategies.  相似文献   

Effects of Fusarium toxins on growth, humoral immune response and internal organs in weaner pigs, and the efficacy of apple pomace as an antidote     

Gutzwiller A  Czeglédi L  Stoll P  Bruckner L 《Journal of animal physiology and animal nutrition》2007,91(9-10):432-438

A feeding trial using 220 weaner pigs which comprised two experimental series was conducted to investigate the effects of diets contaminated with the Fusarium toxins deoxynivalenol (DON) and zearalenone (ZON) and to test the hypothesis that apple pomace acts as an antidote to these mycotoxins. Two diets without contaminated wheat, containing either no pomace or 8% pomace, and two diets with naturally contaminated wheat (3.2 mg DON and 0.06 mg ZON, and 2.1 mg DON and 0.25 mg ZON per kg diet in series 1 and 2 respectively), containing either no pomace or 8% pomace were fed ad libitum for 5 weeks. Mycotoxin exposure lowered feed intake (p < 0.01) and growth (p = 0.05), and tended to decrease the energy conversion ratio (p = 0.06). Although the intake of apple pomace did not increase feed intake, it increased the growth rate (p = 0.04), mainly by restoring growth in the presence of mycotoxins (p = 0.08 for the interaction mycotoxin x pomace). In the first experimental series, the animals were immunized with a parvovirus vaccine. The percentage of seroconverting animals did not differ between the treatments (p = 0.56), which indicates that DON did not affect the humoral immune response. In the second experimental series, female piglets fed the contaminated diets had heavier uteri than piglets fed the uncontaminated diets (p < 0.01), regardless of pomace supplementation. The results show that pomace may alleviate the negative effect of DON on growth but does not counteract the hormonal effects of ZON.  相似文献   

Reported adverse reactions due to veterinary drugs in 2006     

Müntener CR  Bruckner L  Gassner B  Stürer A  Demuth DC  Althaus FR  Zwahlen R 《Schweizer Archiv für Tierheilkunde》2007,149(10):439-448

We received 190 reports of suspected adverse events (SARs) following the use of veterinary drugs for the year 2006: 118 declarations for veterinary drugs and 72 declarations following the application of immunolgical medicinal products. Most of the 118 declarations relate to the use of antiparasitic drugs (48%) and every second declaration to drug use in dogs. Other drug classes concerned were, in decreasing order, antiinfectives (20%) and drugs used off-label (12%; other target species or other indication). For the vaccines, most of the reactions occurred in dogs (62%) followed by horses (11%) and cattle (10%). The most frequently reported reactions concerned the use of a vaccine against piroplasmosis. Another 349 requests for information were processed by the Swiss Toxicological Information center. We also present a case of a serious adverse reaction in form of a Stevens-Johnson syndrome in a cat as well as a case of ketamine abuse. We note the growing interest of practicing veterinarians with pleasure and are currently working on further adaptations to the system.  相似文献   

Environmental Influences on Flour Quality for Sheeted Noodles in Idaho 377s Hard White Wheat     

R. McLean  K. M. O'Brien  L. E. Talbert  P. Bruckner  D. K. Habernicht  M. J. Guttieri  E. J. Souza 《Cereal Chemistry》2005,82(5):559-564

Production of common wheat (Triticum aestivum L.) in the Pacific Northwest of the United States specifically for Asian noodle products is a relatively new goal for grain producers. We surveyed commercial fields of the hard white spring wheat cultivar Idaho 377s in two years to determine the variables contributing to Asian noodle quality and to validate previous observations made with small‐plot research. Fields were surveyed in 1998 and 1999 in two areas of the Snake River Plain of southeastern Idaho separated by ≈100 km, with both irrigated fields and rain‐fed fields sampled in both zones. Samples were evaluated for grain characteristics then milled and evaluated for flour quality, alkaline noodle color, and color and texture of nonalkaline Chinese (salted, neutral pH) noodles. Grain from rain‐fed fields produced brighter and more yellow alkaline noodles than grain from irrigated fields. Grain produced in rain‐fed fields also had lower peak flour pasting viscosity than grain produced in irrigated fields. Flour ash was lowest in grain from rain‐fed fields located in a higher elevation district (Upper Valley) and greatest in grain from irrigated fields located in a lower elevation district (Lower Valley). Noodle hardness and chewiness were greater in Chinese noodles made from grain produced in the Upper Valley than grain from the Lower Valley. Chinese noodle color had significant interaction with the location and irrigation management used for producing the grain. However, Chinese noodle brightness was consistently negatively correlated with flour protein concentration. The color and texture of noodles produced from flours milled from on‐farm commercial production was consistent with previous experiment station small‐plot research.  相似文献