Erythroid progenitors circulating in the blood of adult individuals produce fetal hemoglobin in culture   总被引：7，自引：0，他引：7  

T Papayannopoulou  B Nakamoto  J Buckley  S Kurachi  P E Nute  G Stamatoyannopoulos 《Science (New York, N.Y.)》1978,199(4335):1349-1350

Erythroid colonies, raised from erythroid stem cells circulating in the peripheral blood of normal adult individuals, synthesize considerable amounts of fetal hemoglobin. In cultures from persons with sickling disorders, amounts of hemoglobin F that are known to inhibit sickling in vivo are produced. The results provide evidence that primitive erythroid progenitors are able to express the hemoglobin F production program and that cultures of mononuclear cells of the adult blood can be used to investigate the mechanisms involved in regulation of gamma-globin gene switching.  相似文献   

Productivity is a poor predictor of plant species richness   总被引：5，自引：0，他引：5  

Adler PB  Seabloom EW  Borer ET  Hillebrand H  Hautier Y  Hector A  Harpole WS  O'Halloran LR  Grace JB  Anderson TM  Bakker JD  Biederman LA  Brown CS  Buckley YM  Calabrese LB  Chu CJ  Cleland EE  Collins SL  Cottingham KL  Crawley MJ  Damschen EI  Davies KF  DeCrappeo NM  Fay PA  Firn J  Frater P  Gasarch EI  Gruner DS  Hagenah N  Hille Ris Lambers J  Humphries H  Jin VL  Kay AD  Kirkman KP  Klein JA  Knops JM  La Pierre KJ  Lambrinos JG  Li W  MacDougall AS  McCulley RL  Melbourne BA  Mitchell CE  Moore JL  Morgan JW  Mortensen B 《Science (New York, N.Y.)》2011,333(6050):1750-1753

For more than 30 years, the relationship between net primary productivity and species richness has generated intense debate in ecology about the processes regulating local diversity. The original view, which is still widely accepted, holds that the relationship is hump-shaped, with richness first rising and then declining with increasing productivity. Although recent meta-analyses questioned the generality of hump-shaped patterns, these syntheses have been criticized for failing to account for methodological differences among studies. We addressed such concerns by conducting standardized sampling in 48 herbaceous-dominated plant communities on five continents. We found no clear relationship between productivity and fine-scale (meters(-2)) richness within sites, within regions, or across the globe. Ecologists should focus on fresh, mechanistic approaches to understanding the multivariate links between productivity and richness.  相似文献   

Upwelling: oceanic structure at the edge of the arctic ice pack in winter     

Buckley JR  Gammelsrød T  Johannessen JA  Johannessen OM  Røed LP 《Science (New York, N.Y.)》1979,203(4376):165-167

Observations taken on an expedition into the Arctic Ocean north of Spitsbergen indicated the existence of a region of wind-driven upwelling along the edge of the ice pack. Models underestimate the 12-kilometer width of the upwelling region.  相似文献   

Therapeutic plasma exchange as adjunct therapy in 3 dogs with myasthenia gravis and myasthenia‐like syndrome     

Amber Vitalo  Gareth Buckley  Leonel Londoo 《Journal of Veterinary Emergency and Critical Care》2021,31(1):106-111

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Development of a monoclonal antibody-based cELISA for the analysis of sulfadimethoxine. 2. Evaluation of rapid extraction methods and implications for the analysis of incurred residues in chicken liver tissue     

Muldoon MT  Buckley SA  Deshpande SS  Holtzapple CK  Beier RC  Stanker LH 《Journal of agricultural and food chemistry》2000,48(2):545-550

Several rapid extraction methods were evaluated for use with a monoclonal antibody-based competitive inhibition ELISA (cELISA) to detect sulfadimethoxine (SDM) in chicken liver tissue. These methods included extraction of the samples with (1) aqueous buffer with or without ultrafiltration, (2) acetonitrile/water, (3) methanol/water, or (4) acetone. The organic extraction methods were evaluated with or without solvent evaporation prior to dilution into assay buffer for the cELISA. The aqueous-based extraction methods were compatible with the cELISA. However, of the organic extraction methods, only the acetone liver extract with solvent evaporation prior to analysis was compatible with the cELISA. The cELISA method coupled to aqueous- or acetone-based sample extraction as well as an HPLC method was evaluated for the analysis of chicken liver tissues fortified with SDM at levels from 0.2 to 0.025 ppm. Mean SDM recoveries for the HPLC method and for the cELISA method using samples prepared by aqueous extraction, aqueous extraction and ultrafiltration, or acetone extraction, evaporation, and reconstitution were 68.9, 95.7, 60.1, and 52.5%, respectively. For the analysis of samples obtained from an SDM incurred residue study, HPLC and cELISA analysis of the same organic extract gave results that were highly correlated (R(2) = 0.976; p < 0.0001). However, results obtained from the analysis of aqueous extracts by cELISA did not correlate well with those obtained by HPLC (R(2) = 0.61, p > 0. 0006). This was attributed to the coextraction of cross-reactive SDM-related residues that were not quantified by the HPLC method. The presence of these residues should be considered during data interpretation when ELISA methods coupled with rapid aqueous extraction of samples are used in SDM residue monitoring programs.  相似文献   

Use and conservation of central Australian dunefields   总被引：1，自引：0，他引：1  

R. Buckley 《Biological conservation》1982,22(3):197-205

The arid central Australian dunefields consist largely of alternating swales and longitudinal sandridges whose plant cover and animal populations are dependent on past years' rainfall. The three main uses at present are cattle grazing, oil and mineral exploration, the establishment of reserves and tourism. Constraints on, and implications of, these and additional minor uses and possible future uses are reviewed and assessed with particular reference to conservation.  相似文献   

Electron capture gas chromatographic determination of traces of formaldehyde in milk as the 2,4-dinitrophenylhydrazone     

K E Buckley  L J Fisher  V G MacKay 《Journal of the Association of Official Analytical Chemists》1986,69(4):655-657

A quantitative method is described for the determination of formaldehyde in milk by packed-column gas chromatography and electron capture detection. Aldehyde derivatization was carried out in situ with 2,4-dinitrophenylhydrazine followed by extraction and analysis using an external standard. Average recoveries of 96.3 +/- 1.6% were characteristic of the chromatographic method with an estimated detection limit of 0.026 mg/kg. The technique was applied to determination of formaldehyde in milk from cows consuming a formalin-treated feedstuff.  相似文献   

Randomized, blinded comparison of epinephrine and vasopressin for treatment of naturally occurring cardiopulmonary arrest in dogs     

Buckley GJ  Rozanski EA  Rush JE 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2011,25(6):1334-1340

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Temporal trends in dioxin, furan and polychlorinated biphenyl concentrations in bovine milk from farms adjacent to industrial and chemical installations over a 15 year period     

O'Donovan JV  O'Farrell KJ  O'Mahony P  Buckley JF 《Veterinary journal (London, England : 1997)》2011,190(2):e117-e121

The concentrations of polychlorinated dibenzo-p-dioxins, furans and polychlorinated biphenyls (PCBs) were measured in pooled bovine milk samples collected between 1991 and 2005 in County Cork, Ireland. The pooled samples were of bulk-tank milk collected from farms adjacent to industrial, chemical and pharmaceutical installations (target milk) or from rural farms distant from industrial activity (control milk). Comparing data between the first and last 3-year periods of the study revealed a 62% decrease in the mean total dioxin concentration in target milk from 1.58 to 0.60pg toxic equivalents (TEQ)/g fat. On the same basis the dioxin-like PCB concentration in target milk decreased by 80% over the study period (from 0.95pg to 0.19pg TEQ/g fat). The mean 'marker' PCB concentration in target milk from 1991 to 1993 inclusive was 3359pg/g fat. This value decreased by 75% to a mean of 849pg/g fat for the years 2003-2005 inclusive. The results of this study are consistent with low background dietary/environmental PCB contamination in both target and control herds. The total dioxin concentrations in all samples were well below the maximum tolerable limits permitted for marketable milk. The decrease in the total dioxin concentration in target and control milk samples over the study period was chiefly due to decreases in the concentration of dioxin-like PCBs, consistent with significant reductions in the concentration of PCBs in the dairy cow diet over the 15 year study period.  相似文献   

Community-associated MRSA SCCmec type IVd in Irish equids     

Maeda Y  Millar BC  Loughrey A  Goldsmith CE  Rooney PJ  Moore JE  Rao J  Buckley T  Egan C  Dooley JS  Lowery CJ  Matsuda M 《The Veterinary record》2007,161(1):35-36

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