A BOVINE "TRICHOMONAD CYST."     

H. E. R. Beattik  B.Y.SC  H.D.A. 《Australian veterinary journal》1955,31(6):146-147

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VIBRIOSIS IN DAIRY CATTLE IN SOUTH WEST WESTERN AUSTRALIA.     

L. P. Rikard-Bku  B.V.SC. 《Australian veterinary journal》1954,30(2):41-52

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INVESTIGATION OF SHEEP DIPS     

D. K. O'Neill  M.SC  Ph.D. S. P. Hebdent  B.V.SC.  † 《Australian veterinary journal》1968,44(8):344-349

Groups of 30 sheep of four breeds (Merino, Dorset Horn, Romney Marsh and Border Leicester), that had been maintained under the same environmental and nutritional conditions were used in showering experiments with a diazinon emulsion. The fleeces of all breeds stripped (or depleted) diazinon from the dipping fluid. Diazinon was not deposited evenly down the length of the wool staple. On the Merino fleece, more diazinon was deposited on the outer section and less on the inner section near the skin. On the other breeds, more diazinon was found in the inner and less on the outer sections. Differences in the magnitude and location of the diazinon deposits are discussed, with the aid of supplementary in vitro dipping experiments, in relation to the compactness of the fleece and the amounts and distribution of wax, suint and dirt found there.  相似文献   

STUDIES ON ANTHELMINTICS FOR SHEEP.     

Hugh Mc  L. Gordon B. V. SC 《Australian veterinary journal》1957,33(1):1-7

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FACTORS AFFECTING THE PERFORMANCE OF BEEF CATTLE ON UNIMPROVED PASTURES IN QUEENSLAND     

M. C. Franklin  M.SC  Ph.D. 《Australian veterinary journal》1959,35(4):135-140

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EPIDIDYMITIS OF RAMS     

H. G. Osborne  B.V.SC 《Australian veterinary journal》1955,31(1):11-16

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ENERGY EVALUATION OF POULTRY FEEDSTUFFS   总被引：1，自引：0，他引：1  

W. J. Pryor  M.V.SC  Ph.D.  J. K. Connor  B.Agr.Sc. 《Australian veterinary journal》1966,42(5):141-145

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Tracheal Collapse in the Dog     

Joan A.  O'Brien  V.M.D.  James W.  Buchanan  D.V.M.  D. F. Kelly  M.A.  PH.D.  B.V.SC.  M.R.C.V.S. 《Veterinary radiology & ultrasound》1966,7(1):12-20

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SUBSTANCES IN CALF FAECES TOXIC FOR BOVINE KIDNEY CELLS     

P. B. Spradbrow  B.V.SC.  Ph.D. 《Australian veterinary journal》1965,41(1):17-19

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Optimization of conditions for in vitro production of radical oxygen species and expression of tissue factor by canine mononuclear cells and granulocytes for use in high-throughput assays     

Okano S  Hurley DJ  Bergh MS  Vandenplas ML  Budsberg SC  Moore JN 《Veterinary immunology and immunopathology》2006,112(3-4):234-242

The purpose of this study was to optimize conditions for high throughput measurement of radical oxygen species (ROS) production and expression of tissue factor, also termed procoagulant activity, by canine leukocytes. Granulocytes and mononuclear cells were separated by density gradient centrifugation from peripheral blood collected on several occasions from three healthy large breed dogs. To determine optimal conditions for ROS production, granulocytes were incubated for 1 or 3h in PBG (PBS containing 0.5% BSA and 5mM glucose) or RPMI containing 10% fetal bovine serum (FBS); lipopolysaccharide (LPS), zymosan, peptidoglycan (PGN) and phorbol myristate acetate (PMA) were used as stimuli. ROS was assessed by conversion of the nonfluorescent dye dihydrorhodamine 123 to fluorescent rhodamine 123 by radical species released into the media. To identify optimal conditions for expression of tissue factor, mononuclear cells were incubated for 5h in RPMI containing different concentrations of heat-inactivated FBS (HI-FBS), and LPS, zymosan, PGN or PMA as stimuli. Expression of tissue factor was determined using a one-stage recalcification assay performed in an automated nephelometric coagulation analyzer. Neither LPS nor zymosan increased ROS production by granulocytes incubated in PBG media. In contrast, granulocytes incubated in RPMI had dose-dependent increases in ROS production in response to zymosan and PGN. ROS production was significantly increased by incubation with concentrations of LPS of 0.01microg/ml or greater, and by zymosan concentrations of 0.1microg/ml or greater. ROS production in response to incubation with PMA was significantly increased starting at 10(-7)M, and was significantly greater for cells incubated in RPMI than cells incubated in PBG. LPS-, zymosan- and PGN-stimulated procoagulant activity increased in a dose-dependent manner, whereas PMA-stimulated procoagulant activity peaked at 10(-7)M. Increasing concentrations of HI-FBS significantly increased LPS-, zymosan- and PGN-induced procoagulant activity of mononuclear cells. Results obtained in this study indicate production of ROS by canine granulocytes is optimal when these cells are incubated for 3h in RPMI with LPS (0.1microg/ml), zymosan (10 microg/ml), PGN (10 microg/ml), and PMA (10(-7)M). Furthermore, canine mononuclear cells express procoagulant activity in response to LPS, zymosan, PGN, and PMA, and responses to LPS, zymosan and PGN are enhanced by the addition of HI-FBS. These findings suggest that HI-FBS retains important serum proteins that facilitate interactions between each of these bacterial or yeast derived products and the mononuclear cells. Consequently, future studies regarding the regulation of procoagulant activity by canine mononuclear cells should be performed in the presence of HI-FBS. Both assays utilized in this study allow high throughput of samples, and therefore are appropriate choices for rapid screening of conditions and/or therapeutic interventions affecting the canine inflammatory system.  相似文献