An assessment of genetic diversity and population structures of fifteen Vietnamese indigenous pig breeds for supporting the decision making on conservation strategies     

Van Ba  Nguyen  Nam  Le Quang  Do  Duy Ngoc  Van Hau  Nguyen  Pham  Lan Doan 《Tropical animal health and production》2020,52(3):1033-1041

Tropical Animal Health and Production - The study aimed to characterize genetic diversity, genetic clusters, and phylogenetic relationships of 15 Vietnamese indigenous pig breeds across the country...  相似文献   

Culture-based fisheries in small reservoirs in northern Vietnam: effect of stocking density and species combinations     

Hai Son Nguyen  Anh T Bui  Dieu Q Nguyen  Dung Q Truong  Luu Thanh Le  Nigel W Abery  & Sena S De Silva 《Aquaculture Research》2005,36(11):1037-1048

This study was conducted in 20 reservoirs, ranging in size from 4 to 30 ha, in the mountainous, northern region of Vietnam, in ThaiNguyen and YenBai provinces, over two growth cycles in 2002/03 and 2003/04. The reservoirs are leased by farmers for fishery activities from the provincial administration, and the trials were managed by the lessee farmers. Three species combinations in ratios (by fingerling weight) of grass carp: silver carp: bighead carp: common carp: mrigal 1:2:1:1:3 (A), 1:3:1:1:2 (B) and 1:2:1:1:2 (C) were used as seed stock. The overall mean yield of stocked fish in 2002/03 and 2003/04 growth cycles in reservoirs in ThaiNguyen and YenBai provinces was 165 (±21) and 190 (39), and 287 (±22) and 325 (±24) kg ha^?1 respectively. The yield in reservoirs in both provinces, in both growth cycles and irrespective of the species combinations, increased in relation to stocking density. In reservoirs in ThaiNguyen Province, the species combination B gave the lowest yield (both growth cycles and overall), and differed significantly (P<0.05) from combinations A and C. The stocking efficiency (ratio of the yield of stocked fish in kg ha^?1 to the weight of the stocked fish in kg ha^?1) in reservoirs in ThaiNguyen Province ranged from 2.9 to 5.1 over the two growth cycles and that in YenBai from 2.8 to 3.9. There was no discernible trend between growth cycles and/or between species combinations. The major cost incurred was for fingerling procurement. In all instances, a net profit was accrued. The mean (±standard error (SE)) net profit ha^?1 (in 10³VN dong; approximately 15 500 VND=1 US$) was 885 (±270) and 864 (±214), and 1322 (±176) and 1600 (±150) for the growth cycles 2002/03 and 2003/04 for reservoirs in ThaiNguyen and YenBai provinces respectively. Between the two growth cycles, the net profit increased in eight and three reservoirs from YenBai and ThaiNguyen, respectively, the maximum increase being recorded in Khuan Gio (165%) and Dong Man (39%) reservoirs.  相似文献   

Carotenoid accumulation and characterization of cDNAs encoding phytoene synthase and phytoene desaturase in garlic (Allium sativum)     

Tuan PA  Kim JK  Kim HH  Lee SY  Park NI  Park SU 《Journal of agricultural and food chemistry》2011,59(10):5412-5417

Phytoene synthase (PSY) and phytoene desaturase (PDS), which catalyze the first and second steps of the carotenoid biosynthetic pathway, respectively, are key enzymes for the accumulation of carotenoids in many plants. We isolated 2 partial cDNAs encoding PSY (AsPSY-1 and AsPSY-2) and a partial cDNA encoding PDS (AsPDS) from Allium sativum. They shared high sequence identity and conserved motifs with other orthologous genes. Quantitative real-time PCR analysis was used to determine the expression levels of AsPSY1, AsPSY2, and AsPDS in the bulbils, scapes, leaves, stems, bulbs, and roots of garlic. High-performance liquid chromatography demonstrated that carotenoids were not biosynthesized in the underground organs (roots and bulbs), but were very abundant in the photosynthetic organs (leaves) of A. sativum. A significantly higher amount of β-carotene (73.44 μg·g(-1)) was detected in the leaves of A. sativum than in the other organs.  相似文献   

虚拟现实技术在测绘中的应用     

Doan Van Chinh  Bui Thi Kien Trinh 《湖南农机》2011,38(1)

虚拟现实技术是门新兴的科学技术,是多门学科交叉的产物,将虚拟现实技术引入到测绘领域,实现地理信息的三维可视化与虚拟再现,为测绘学的发展指明了新的方向.本文介绍了虚拟现实的概念及特征,分析了虚拟现实实现的关键技术以及虚拟现实系统的分类,并就虚拟现实技术在测绘领域中应用提出观点.  相似文献   

Improvement of porcine oocytes with low developmental ability after fusion of cytoplasmic fragments prepared by serial centrifugation     

Viet Linh N  Kikuchi K  Nakai M  Noguchi J  Kaneko H  Dang-Nguyen TQ  Maedomari N  Nguyen BX  Nagai T  Manabe N 《The Journal of reproduction and development》2011,57(5):620-626

We have shown in pigs that oocytes denuded of cumulus cells at 24 h of in vitro maturation culture and subsequently matured for a total of 46 h (DO24 oocytes) have lower cytoplasmic maturity than those matured with cumulus cells for 46 h and then denuded (DO46 oocytes). In the present study, DO24 zona-free oocytes were fused with one (1C) or two (2C) cytoplasmic fragments produced by serial centrifugation ("centri-fusion") of DO46 oocytes (DO24+1C and DO24+2C oocytes, respectively). Groups of (1) DO46 (a control), (2) DO24, (3) DO24+1C and (4) DO24+2C oocytes were partheno-activated by an electrical pulse or fertilized in vitro and subsequently cultured for 6 days. In the fused groups, female pronucleus (FPN) formation rates were higher than that in the DO24 group after parthenogenetic activation (PA); however, the blastocyst rates were intermediate between those of the control and DO24 groups. After in vitro fertilization, the male pronucleus (MPN) formation rates in the fused groups were similar to that in the control group and higher than that in the DO24 group; the normal fertilization rate in the DO24+2C group was higher than that in the DO24 group and similar to that in the control group, resulting in significantly higher blastocyst rates in the DO24+2C and control groups than that in the DO24 group. These results suggest that centri-fusion using ooplasm from fully matured DO46 oocytes can offer a potentially novel approach for restoration of cytoplasmic maturity to oocytes with low developmental ability and subsequent improvement of fertilization and developmental competence.  相似文献   

The effect of ovary storage and in vitro maturation on mRNA levels in bovine oocytes; a possible impact of maternal ATP1A1 on blastocyst development in slaughterhouse-derived oocytes     

Somfai T  Imai K  Kaneda M  Akagi S  Watanabe S  Haraguchi S  Mizutani E  Dang-Nguyen TQ  Inaba Y  Geshi M  Nagai T 《The Journal of reproduction and development》2011,57(6):723-730

Since BSE testing of slaughtered cattle is obligatory in Japan, storage of ovaries at 15-20 C overnight in phosphate buffered saline has become a routine protocol in in vitro production (IVP) of cattle embryos. Ovary storage is known to reduce developmental competence of oocytes; however, its effects on oocyte gene expression have not been clarified yet. This study compared oocytes collected from stored slaughterhouse-derived ovaries with those collected by Ovum Pick-Up (OPU) in terms of the expression of 20 selected genes to determine if ovary storage affects cellular processes at the molecular level. Expression of mRNA in oocytes was assayed before and after in vitro maturation (IVM) by real-time quantitative PCR. Maternal mRNA levels of genes were investigated in 2-cell stage embryos obtained from slaughterhouse oocytes to assess their roles for blastocyst formation. In immature OPU oocytes, genes related to metabolism (GAPDH), transporters (GLUT8, ATP1A1) and stress resistance protein (HSP70) showed significantly higher expression compared with oocytes derived from stored ovaries. During IVM, the expression of GDF9, GLUT8, CTNNB1 and PMSB1 was significantly decreased irrespective of oocyte source. Two-cell stage embryos cleaving at 22-25 h after in vitro fertilization (IVF) showed a significantly higher blastocyst formation rate and ATP1A1 gene expression level compared with those cleaving at 27-30 h after IVF. Our results reveal that storage of ovaries alters mRNA levels in oocytes. Correlation of Na/K ATPase ATP1A1 expression in IVP embryos at the 2-cell and 8-cell stages with their developmental ability to the blastocyst stage may suggest the importance of maternal mRNA of this gene during blastulation in embryos derived from slaughterhouse oocytes.  相似文献   

Influence of the semen deposition site on the calves' sex ratio in Simmental dairy cattle     

Zobel R  Gereš D  Pipal I  Buić V  Gračner D  Tkalcic S 《Reproduction in domestic animals》2011,46(4):595-601

It has been suggested that the time of insemination has effect on the calves' sex ratio because of the differences in timing of capacitation, motility and survival time of the X and Y spermatozoa in the female reproductive tract. We have conducted a field trial to study the effects of different semen deposition sites on the sex ratio and fertility in cattle. Two groups of 450 cows were inseminated via artificial insemination: group A was inseminated into the uterine body and group B was inseminated deep into the uterine horn ipsilateral to the ovary with dominant follicle. After applying several exclusion criteria, a total of 607 pregnant cows were considered for data analysis (group A = 318 and group B = 289 cows). The conception rate was 7% higher (p < 0.05) in the group A, with 23% more of the male calf pregnancies (p < 0.001). At the same time, 18% more of the female calves were calved in the group B (p < 0.005). The difference in male calves between the two groups was 21% and in female calves was 20% (p < 0.001). We conclude that semen deposition site plays a significant role in differences in gender ratio observed after calving. Intracornual semen deposition resulted in a higher ratio of female calves, whereas uterine body deposition site resulted in higher male calves ratio, probably contributing physiologically to the differences in motility, capacitation time, the lifespan of X vs Y spermatozoa and to the pronounced shift of X spermatozoa in the female genital tract.  相似文献   

Genetic characterization of coconut (Cocos nucifera L.) varieties conserved in Vietnam through SCoT marker-based polymorphisms     

Khang  Nguyen Hoang Minh  Quang  Nguyen Thien  Mai  Hoang Ngoc Xuan  Phuong  Nguyen Doan Nguyen  Thao  Nguyen Phuong  Quoc  Nguyen Bao 《Genetic Resources and Crop Evolution》2022,69(1):385-398

Genetic Resources and Crop Evolution - Coconut (Cocos nucifera L.) production sustains millions of smallholder farmers and their families around the globe, and also powers a thriving industry of...  相似文献   

Characterizing morphological traits and estimating genetic relationship for intermediate soybean collected from South Korea     

Dang Toan Vu  Kwang‐Hyun Baek  La Tuan Nghia  Euiho Park 《Plant Breeding》2013,132(3):324-329

Twenty‐two wild, 16 intermediate and 20 cultivated soybean varieties were used to analyse relationships among the species of subgenus Soja, genus Glycine using 11 morphological traits and simple sequence repeat (SSR) markers. These genotypes using eleven agronomic characteristics were divided into three clusters: cluster I included 20 G. max, cluster II included 12 intermediate, and cluster III included 22 G. soja and four intermediate lines. Genetic relationships among the species of subgenus Soja showed three distinct clusters. Cluster I consisted of 20 G. max cultivars and two intermediate type lines, and cluster II consisted of 13 intermediate type and two G. soja lines; however, cluster III consisted of 20 G. soja and one intermediate type lines. These phenotypic and genetic data suggest that the intermediate type lines could be distinguished between G. max and G. soja lines. However, the intermediate type could not be classified as a new species.  相似文献   

Effects of replacing fish meal with soy cake in a diet based on urea-treated rice straw on performance of growing Laisind beef cattle   总被引：1，自引：1，他引：0  

Duong Van Nhiem  Jan Berg  Nils Petter Kjos  Nguyen Xuan Trach  Bui Quang Tuan 《Tropical animal health and production》2013,45(4):901-909

The present study was to test if different ratios of fish meal to soy cake as protein supplements in the diet are not significantly different in effects on feed intakes and performance of growing beef cattle fed urea-treated rice straw (URTRS) as basal diet under local conditions in North Vietnam. Thirty-six male cattle of Laisind (50 % local Yellow cattle and 50 % Sindhi, both Bos indicus) at around 12 months of age with an average live weight of 124?±?12 kg at start were used in six groups of the experiment. Six diets named A, B, C, D, E, and F used in the experiment were similar in crude protein (CP) level, except for diet A with no supplementation, but different in fish meal/soy cake ratio based on CP content as follows: diet A 0/0, diet B 100/0, diet C 75/25, diet D 50/50, diet E 25/75, and diet F 0/100. Following a 4-week adaptation period, a comparison period lasted 24 weeks. The total intakes of dry matter (DM), DM from roughage, metabolizable energy (ME), and CP were 6, 7, 6, and 20 % higher in diets B–F than diet A with P values ≤0.05, 0.05, 0.05, and 0.001, respectively. The average daily gain (ADG) was 50 % higher (P?<?0.001) in diets B–F than that in diet A. Compared to diet A, diets B–F had lower feed conversion ratio (FCR)_DM, FCR_ME, and FCR_CP by 30, 20, and 30 %, respectively (all at P?<?0.001). There were no significant differences between diets B–F in the intakes, FCRs, and ADG. The apparent digestibility of CP was 12 to 24 % higher (P?<?0.001) in diets B–F than that in diet A. There was a tendency that the apparent digestibilities of DM, CP, and CF were increasing with increasing level of soy cake in this study. The ratio of fish meal to soy cake did not significantly affect feed intakes, ADG, FCR, and apparent nutrient digestibilities of the cattle. Soy cake can therefore be used to partially or fully replace fish meal as a protein supplement in the diet of growing beef cattle fed URTRS without any negative effects on animal performance.  相似文献