Baumkrone und Schaftzuwachs     

Professor Dr. Busse 《European Journal of Forest Research》1930,52(7-8):310-318

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Biochemical differentiation of Listeria from cheese     

H Seiler  M Busse 《Berliner und Münchener tier?rztliche Wochenschrift》1989,102(5):166-170

Listeria spp. isolated from cheese were tested for biochemical characteristics together with reference strains from culture collections. Microtitration plates were used for testing the fermentation patterns. The results were subjected to a numerical cluster analysis based on linkage maps. The variation of the group structures calculating the characteristics with and without the hemolysin reactions is demonstrated. The pathogenic species L. monocytogenes could only be separated from the avirulent species L. innocua by the hemolysin tests. Most of the cheese isolates were identified as L. innocua, some as L. monocytogenes and L. seeligeri. There is a need for an inexpensive commercial test kit to identify the serovars or virulence factors of Listeria spp. in the quality assessment of food. The present study sets up doubts for a sufficiently ensured separation of L. innocua from L. monocytogenes.  相似文献   

Ectomycorrhizal Formation in Herbicide-Treated Soils of Differing Clay and Organic Matter Content     

Busse  Matt D.  Fiddler  Gary O.  Ratcliff  Alice W. 《Water, air, and soil pollution》2004,152(1-4):23-34

Herbicides are commonly used on private timberlands in the western United States for site preparation and control of competing vegetation. How non-target soil biota respond to herbicide applications, however, is not thoroughly understood. We tested theeffects of triclorpyr, imazapyr, and sulfometuron methyl on ectomycorrhizal formation in a greenhouse study. Ponderosa pine, Douglas-fir, and white fir seedlings were grown in four forest soils ranging in clay content from 9 to 33% and organic matter content from 3 to 17%, and treated with commercial formulations of each herbicide at 0, 1.0, and 2.0 times the recommended field rate. Many of the possible herbicide-soil combinations resulted in reduced seedling growth. Root development was particularly sensitive to the three herbicides, with an average of 51% fewer root tips compared to the control treatment. The ability of mycorrhizal fungi to infectthe remaining root tips, however, was uninhibited. Mycorrhizal formation was high, averaging 91% of all root tips, regardless of herbicide, application rate, soil type, or conifer species. In agreement, soil microbial biomass and respiratory activity were unaffected by the herbicide treatments. The results show that these herbicides do not alter the capability of mycorrhizal fungi to infect roots, even at concentrations detrimental to seedling growth.  相似文献   

Effect of Roscovitine‐Treated Donor Cells on Development of Porcine Cloned Embryos     

HJ Park  OJ Koo  DK Kwon  JT Kang  G Jang  BC Lee 《Reproduction in domestic animals》2010,45(6):1082-1088

Synchronization of the donor cell cycle is an important factor for successful animal cloning by nuclear transfer. To improve the efficiency of porcine cloning, in the present report, we evaluated effects of contact inhibition, serum starvation and roscovitine treatment of donor cells on in vitro and in vivo developmental potency of cloned porcine embryos. Fibroblasts derived from a porcine foetus at day 30 of gestation were isolated and cultured to 70% confluency. Then, cells were either cultured to 100% confluency for contact inhibition, or cultured in 0.5% serum for 72 h for serum starvation or with 15 μm roscovitine for 24 h. Cells were most effectively synchronized at G0/G1 in the serum starvation group (87.5%) compared with the contact inhibition and roscovitine treatment groups (76.3% and 79.9% respectively p < 0.05). However, after somatic cell nuclear transfer followed by in vitro culture, the serum starvation group showed a significantly lower blastocyst formation rate (5.6%) compared with the contact inhibition and roscovitine treatment groups (11.6% and 20.0% respectively). Differential expression of apoptosis‐related genes and the level of apoptosis in each treatment group explain the variation in developmental competence among the groups. Significantly higher level of apoptosis was observed in the serum starvation group. On the other hand, the roscovitine treatment group shows the lowest level of apoptosis and the best in vitro development among the groups. Cloned embryos derived from roscovitine‐treated donor cells were transferred to surrogate pigs. Three healthy live piglets were produced. In conclusion, we suggest that roscovitine treatment of donor cells improves development of cloned porcine embryos and can raise the efficiency of cloned piglet production.  相似文献   

Clinical,Ultrasonographic and Pathological Findings in a Bull with Segmental Aplasia of the Mesonephric Duct     

HJ Williams  SG Revell  SFE Scholes  AE Courtenay  RF Smith 《Reproduction in domestic animals》2010,45(5):e212-e216

On assessment for use in an AI stud, a 12‐month‐old bull was found to produce low volume ejaculates with 41% of the sperm having morphological abnormalities. No left epididymal tail was palpable and the head of the epididymis on the left was twice the size compared with the right. Ultrasound examination showed the left testis to contain a large central area of decreased echogenicity, which could be followed proximally to a 15‐mm echolucent lesion at the site of the epididymal head. Postmortem examination revealed a 15‐mm diameter cyst in the region of the left epididymal head, and absence of the body and tail of the epididymis. The mediastinum testis of the left testis was dilated, corresponding to the area of decreased echogenicity observed on ultrasonography. No left seminal vesicle was present and the ampulla was significantly smaller than the same structure on the right. Histological examination revealed incomplete or absent spermatogenesis involving the majority of seminiferous tubules in the left testis, and a small proportion of those of the right testis. The cystic structure at the site of the left epididymal head was lined by irregular, sometimes attenuated, epithelium and contained sparse spermatozoa. This case demonstrates the adverse impact, which segmental aplasia of the mesonephric duct had on the testicular and epididymal function of a bull, and highlights the importance of careful clinical assessment in its diagnosis.  相似文献   

Selecting trees for urban paved sites in Scandinavia – A review of information on stress tolerance and its relation to the requirements of tree planners     

Henrik Sjman  Anders Busse Nielsen 《Urban Forestry & Urban Greening》2010,9(4):281-293

Urban paved sites are complex stress environments and when selecting trees for such sites, tree planners must prioritise stress tolerance above aesthetic appeal and functional aspects. This requires detailed information about the tolerance of tree species to environmental stresses, so as to support urban tree planners in selecting a wide range of trees. In Scandinavia there is currently no overview of the extent to which the character of information about stress tolerance and its dissemination in books and papers support urban tree planners in selecting a wide range of species for paved sites. Books on dendrology, literature on plant use in cities, tree nursery catalogues and scientific papers were therefore reviewed. For the review, eight tree species were selected along a gradient representing their intensity of use in Northern Europe. We examined the character of information and assessed it relative to tree planners’ requirements for information to be; contextual, local to Scandinavia, referring to existing plantings in paved sites and recommendations for use of the species in paved sites. The results showed that existing information is piecemeal and that most is either too general (dendrology literature) or too specific or contradictory (scientific literature) to meet the requirements of urban tree planners, while books intended for plant use in cities do not sufficiently integrate the local perspective. Moreover, contextual information local to the Scandinavian region is mainly provided for already much used species. These findings led to suggestions on how future urban forestry and arboriculture research and dissemination efforts in Scandinavia can encourage tree planners to use a greater variety of tree species in urban paved sites.  相似文献   

The Effect of Pasture Nitrate Concentration and Concentrate Intake after Turnout on Embryo Growth and Viability in the Lactating Dairy Cow     

RA Laven  HJ Biggadike  & RD Allison 《Reproduction in domestic animals》2002,37(2):111-115

This study investigated the effects on embryo growth and survival rate of feeding heavily‐fertilised spring grass, containing high levels of quickly‐degradable nitrogen, to pregnant cows. Forty‐eight lactating Holstein cows between 2 and 8 weeks pregnant were turned‐out, after a one‐week transition period onto high‐ or low‐nitrate pasture and fed a high‐ or low‐concentrate supplement. Cows grazing the High nitrate pasture had significantly higher milk and plasma urea concentrations than cows grazing the Control pasture, while cows which were fed less concentrate had a notably higher plasma ammonia. However, there was no evidence that an increased quickly‐degradable nitrogen (QDN) intake from pasture affected embryo survival or growth from 20 days onwards. This suggests that the impact of turnout on fertility mainly affects ovulation, fertilisation and/or the early embryo.  相似文献   

A Review of Methods Used in the Laboratory Diagnosis of Fireblight1)     

HJ. MILLER 《EPPO Bulletin》1979,9(1):7-11

Most well–known laboratory methods which can be used in the diagnosis of fireblight, as well as more recent developments in identification, have been discussed. They vary from the use of simple culture media to the use of immunofluorescent microscopy. The use of any one method alone is not advised, but by combining methods, accurate and rapid diagnosis is possible. In order to minimize diagnostic errors, especially among less experienced workers, the use of 5 % sucrose–nutrient agar for bacterial isolation followed by serological control is recommended. For further information reference is given particularly to the procedures described by Lelliott at the first EPPO Conference on Fireblight held in 1967 at Canterbury.  相似文献   

Convection in a rotating layer: a simple case of turbulence     

Busse FH  Heikes KE 《Science (New York, N.Y.)》1980,208(4440):173-175

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The effect of prophylactic topical carbonic anhydrase inhibitors in canine primary closed‐angle glaucoma          下载免费PDF全文

R. Stavinohova  J. R. Newton  C. Busse 《The Journal of small animal practice》2015,56(11):662-666

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