Immune response to avian reovirus in chickens and protection against experimental infection     

J. MEANGER  R. WICKRAMASINGHE  CE ENRIQUEZ  GE WILCOX 《Australian veterinary journal》1997,75(6):428-432

Objectives To assess the efficacy of the vaccination procedure and the effect of the transfer of maternal antibodies to progeny chickens on reovirus pathogenicity.
Design To vaccinate chickens and challenge progeny chickens with high doses of homologous and heterologous viruses.
Procedure High doses of reovirus strains RAM-1, 1091 and 724 were used to induce tenosynovitis lesions. High doses were produced by concentration of viruses grown in cell culture. Then similar doses of viruses were used to challenge immunised chickens progeny.
Result Vaccination of breeding hens with the RAM-1 strain of avian reovirus, which resulted in the passive transfer of neutralising antibody to progeny chickens, completely prevented the development of tenosynovitis in 80% of progeny chickens infected with the homologous virus. Even though multiple injection of hens resulted in broadening of the normal type-specificity of the neutralising antibody response against heterologous serotypes of avian reovirus, only marginal protection against strains of two heterologous serotypes of avian reovirus was obtained.
Conclusions A model for assessing the efficacy of vaccination against avian reovirus strains on clinical signs such as tenosynovitis was developed that overcome the normal low virulence of Australian strains of avian reovirus. Breeding hens can be immunised with Australian strain of avian reovirus with passive transfer of antibody via the yolk to the progeny chickens. Although the neutralising antibody response to three injections of inactivated virus decreased the specificity of the neutralising antibody response against antigenically heterologous strains of avian reovirus, the protective immunity appeared to retain type-specificity.  相似文献   

Tendonitis of the branches of insertion of the superficial digital flexor tendon in horses     

K T GIBSON  H M BURBIDGE  B H ANDERSON 《Australian veterinary journal》1997,75(4):253-256

Objective To describe clinical findings, ultrasonographic features and outcome of injury to the branches of insertion of the superficial digital flexor (SDF) tendon in horses.
Design Retrospective study of 14 cases.
Procedure Fourteen Thoroughbred horses with tendonitis affecting the branches of insertion of the SDF tendon were examined for lameness, location and amount of swelling, and the presence of other musculoskeletal abnormalities. The flexor tendons were assessed by ultrasonographic examination, and recommendations were made for management of the cases. Outcome was assessed by re-examination of some horses, direct communication with the owner or trainer, and examination of race records.
Results The lateral SDF branch was affected in 10 horses; the medial branch in three, and both branches in one horse. Two horses had concurrent injuries to the SDF tendon in the metacarpal region of the contralateral limb. Ultrasonographic findings included swelling of the affected SDF branch, peritendinous fluid accumulation, disruption of normal fibre alignment on sagittal scan, and variable loss of echogenicity. As healing occurred, there was return of normal echogenicity, but normal fibre alignment did not return completely and apparent adhesions formed between the affected SDF branch and adjacent structures. Seven of 10 horses which returned to their previous use were able to compete without further tendon injury. Recurrence of injury occurred in one case, and another two horses developed tendonitis in the metacarpal region. One horse was retired from racing but was able to compete at dressage without recurrence of injury. Two horses were retired for breeding without returning to training, and one horse was sold and lost to follow up but did not race.
Conclusion The prognosis is fair for return to previous use following injury to the branches of insertion of the SDF tendon in athletic horses.  相似文献   

Field evaluation of a mixture of albendazole sulphoxide and levamisole against Ostertagia and Trichostrongylus spp in sheep     

N. ANDERSON  PJ MARTIN  RG JARRETT† 《Australian veterinary journal》1991,68(4):133-136

The efficacy of a mixture of albendazole sulphoxide and levamisole, 3.6 and 8.25 mg/kg respectively, at single and double dose rates, was compared with the recommended dose rate of each anthelmintic alone. The comparison was conducted on groups of 6 to 14-week-old lambs on 22 farms, 16 of which had evidence of multiple resistance to benzimidazole and levamisole. A single dose of the mixture reduced mean egg counts by 95% on half the farms with multiple resistance and on all the remaining farms. Consequently, the mixture should be included in an assessment of effective anthelmintics on farms to determine its value for nematode control. A double dose rate of mixture was effective on all but 4 farms. Reductions caused by the mixture were due to the additive actions of the drugs on 18 of 22 farms; synergistic action was noted on only 3 farms. It was concluded that the mixture of albendazole sulphoxide and levamisole offered many farmers an effective anthelmintic for use in preventive control programs. Recommendations for such programs include annual rotation of effective anthelmintics as a means of delaying selection for drug resistance.  相似文献   

THE APPLICATION OF THE ROSE-BENGAL PLATE TEST TO SERUMS COLLECTED FROM CARCASES AT ABATTOIRS     

I. D. WELLS  B.V.Sc.    B. ANDERSON  D. DANIEL 《Australian veterinary journal》1974,50(3):128-128

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Water balance in the dog and cat     

R. S. ANDERSON 《The Journal of small animal practice》1982,23(9):588-598

In respect of its metabolic control of water balance, the cat probably differs as much from the dog as it does in most other respects. In qualitative terms the routes of gain and loss of water are very much the same: in quantitative terms and in its homeostatic response to different environmental stimuli or insults the cat and the dog are rather different. Dogs and cats are among the commonest species with which veterinarians have to deal. They share much the same environment and are treated in much the same way by their owners. They are almost interchangeable as pets. So far as the veterinary profession is concerned, there are a few outstanding differences such as their response to certain drugs, their reproductive behaviour and their range of aggressive and defensive weapons—but they are in other ways treated in a somewhat similar fashion. It is, however, only within recent years that some other outstanding differences have really become identified between the two species. These differences are in aspects of protein, fat and vitamin metabolism which have direct or indirect relevance to feeding cats and dogs. But they are also manifest in water metabolism, which is the subject of this paper. The first part of this paper will deal with some differences in the water metabolism of dogs and cats which are in the scientific literature but are not widely known outside the research field. The second part will describe some of the work which we have been doing on food/water interrelationships of the dog and cat.  相似文献   

Evaluation of the Brix refractometer as an on‐farm tool for the detection of passive transfer of immunity in dairy calves          下载免费PDF全文

JB Thornhill  GL Krebs  CE Petzel 《Australian veterinary journal》2015,93(1-2):26-30

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Comparative Development of Embryonic Age by Organogenesis in Domestic Dogs and Cats          下载免费PDF全文

NCG Pieri  AF Souza  JB Casals  KCS Roballo  CE Ambrósio  DS Martins 《Reproduction in domestic animals》2015,50(4):625-631

The precise determination of the embryonic chronology is very important in reproductive biotechnologies, especially in estimating embryonic age. Thus, there is a need for greater knowledge and standardization for determining the chronology of embryonic development and functional morphology. We describe aspects of embryonic development in two domestic carnivores to add knowledge about organ peculiarities and for application in veterinary practice, in prenatal development and in the biotechnology fields. We found that the development of differential characteristics of embryonic organs occurs in the first trimester of pregnancy for both species. Thus, using the combination of the crown‐rump length, macroscopic analysis and optical microscopy, it is possible to predict gestational age more precisely in animals that lack a defined breed and establish an embryonic pattern.  相似文献   

Estimating the mass of asteroid 253 mathilde from tracking data during the NEAR flyby   总被引：1，自引：0，他引：1  

DK Yeomans  J Barriot  DW Dunham  RW Farquhar  JD Giorgini  CE Helfrich  AS Konopliv  JV McAdams  JK Miller  WM Owen  DJ Scheeres  SP Synnott  BG Williams 《Science (New York, N.Y.)》1997,278(5346):2106-2109

The terminal navigation of the Near Earth Asteroid Rendezvous (NEAR) spacecraft during its close flyby of asteroid 253 Mathilde involved coordinated efforts to determine the heliocentric orbits of the spacecraft and Mathilde and then to determine the relative trajectory of the spacecraft with respect to Mathilde. The gravitational perturbation of Mathilde on the passing spacecraft was apparent in the spacecraft tracking data. As a result of the accurate targeting achieved, these data could be used to determine Mathilde's mass as 1.033 (+/- 0.044) x 10(20) grams. Coupled with a volume estimate provided by the NEAR imaging team, this mass suggests a low bulk density for Mathilde of 1.3 grams per cubic centimeter.  相似文献   

AN ASSESSMENT OF THE RELATIONSHIP BETWEEN THE SURFACE PROPERTIES OF CHALK SOILS AND SLOPE FORM USING PRINCIPAL COMPONENTS ANALYSIS     

K. E. ANDERSON  P. A. FURLEY 《European Journal of Soil Science》1975,26(2):130-143

Five line transects were sited over grass-covered, convex-concave slopes in the Berkshire and Wiltshire chalk downs, with the object of describing and interpreting the variation in selected surface soil properties. Previous work had concentrated upon the correlation between single variables and slope gradient and slope length, whereas the emphasis of the present paper lies in examining the interrelationships amongst the variables through the use of principal components analysis. A consistent pattern of variation was established between the five sites. The first component derived from the analysis was interpreted as a combined organic matter and soluble constituents factor, which accounted for between 50 and 60 per cent of the total variance. The second component was identified as particle-size variation and accounted for a further 13 to 18 per cent of the total variance. Whereas the distribution of the component scores for the second component supported the original idea of a sharp junction effect in the pattern of soil properties on the slope, the first and most important component indicated a more diffuse transition from net erosion to net deposition conditions.  相似文献   

The effect of select seminal plasma proteins on endometrial mRNA cytokine expression in mares susceptible to persistent mating‐induced endometritis          下载免费PDF全文

CE Fedorka  KE Scoggin  EM Woodward  EL Squires  BA Ball  MHT Troedsson 《Reproduction in domestic animals》2017,52(1):89-96

In the horse, breeding induces a transient endometrial inflammation. A subset of mares are unable to resolve this inflammation, and they are considered susceptible to persistent mating‐induced endometritis PMIE Select seminal plasma proteins cysteine‐rich secretory protein‐3 (CRISP‐3) and lactoferrin have been shown to affect the innate immune response to sperm in vitro. The objective of this study was to determine whether the addition of CRISP‐3 and lactoferrin at the time of insemination had an effect on the mRNA expression of endometrial cytokines in susceptible mares after breeding. Six mares classified as susceptible to PMIE were inseminated during four consecutive oestrous cycles with treatments in randomized order of: 1 mg/ml CRISP‐3, 150 μg/ml lactoferrin, seminal plasma (positive control) or lactated Ringer's solution (LRS; negative control) to a total volume of 10 ml combined with 1 × 10⁹ spermatozoa pooled from two stallions. Six hours after treatment, an endometrial biopsy was obtained for qPCR analysis of selected genes associated with inflammation (pro‐inflammatory cytokines interleukin (IL)‐1β, IL‐8, tumour necrosis factor (TNF)‐α, interferon (INF)‐γ, anti‐inflammatory cytokines IL‐1RN and IL‐10, and inflammatory‐modulating cytokine IL‐6). Seminal plasma treatment increased the mRNA expression of IL‐1β (p = .019) and IL‐8 (p = .0068), while suppressing the mRNA expression of TNF (p = .0013). Lactoferrin also suppressed the mRNA expression of TNF (p = .0013). In conclusion, exogenous lactoferrin may be considered as one modulator of the complex series of events resulting in the poorly regulated pro‐inflammatory response seen in susceptible mares.  相似文献