Effects of Retinoids on the In Vitro Development of Capra Hircus Embryos to Blastocysts in Two Different Culture Systems     

A Chiamenti  CR Aguiar Filho  LM Freitas Neto  RM Chaves  FF Paula‐Lopes  PF Lima  PBD Gonçalves  MAL Oliveira 《Reproduction in domestic animals》2010,45(5):e68-e72

The objective of this study was to evaluate the effect of retinol (RT) and retinoic acid (RA) on the in vitro development of pre‐implantation goat embryos cultured in potassium simplex optimized medium or synthetic oviduct fluid or cocultured in oviductal cells monolayer either in potassium simplex optimized medium or synthetic oviduct fluid. A total of 2407 cumulus‐oocyte complexes were aspirated from 2 to 6 mm ovarian follicles from slaughtered animals. Selected cumulus‐oocyte complexes were subjected to in vitro maturation in TCM 199 for 24 h at 39°C in an atmosphere of 5% (v/v) CO₂ in humidified air. In vitro fertilization was performed in modified defined medium. Eighteen hours after in vitro fertilization, cumulus cells were removed and presumptive zygotes were randomly distributed into experimental groups. In Experiment 1, presumptive zygotes were cultured in potassium simplex optimized medium, potassium simplex optimized medium + RT, potassium simplex optimized medium + retinoic acid, synthetic oviduct fluid, synthetic oviduct fluid + RT and synthetic oviduct fluid + RA at 39°C in a humidified atmosphere of 5% (v/v) CO₂, 5% (v/v) O₂ and 90% (v/v) N₂. In Experiment 2, presumptive zygotes were cocultured in potassium simplex optimized medium + oviductal cells monolayer, potassium simplex optimized medium + RT + oviductal cells monolayer, potassium simplex optimized medium + RA + oviductal cells monolayer, synthetic oviduct fluid + oviductal cells monolayer, synthetic oviduct fluid + RT + oviductal cells monolayer and synthetic oviduct fluid + RA + oviductal cells monolayer in an atmosphere of 5% (v/v) CO₂ in humidified air. In both experiments, media were partially changed on day 2 after in vitro fertilization and unfertilized oocytes were excluded from the experiment. Embryos were cultured or cocultured for 8 days. In Experiment 1, there was no effect of RT or RA supplementation on the proportion of oocytes that reached the morula or blastocyst stages. By contrast, Experiment 2 demonstrated that the addition of 0.28 μg/ml RT and 0.5 μm RA to the embryo culture media stimulated (p < 0.05) development to the morula and blastocyst stages under the coculture conditions tested. In conclusion, retinoids play an important role in pre‐implantation development of goat embryos and can be used to enhance in vitro embryo production.  相似文献   

Characterization of structure and function of the mouse retina using pattern electroretinography, pupil light reflex, and optical coherence tomography     

Mohan K  Harper MM  Kecova H  Ye EA  Lazic T  Sakaguchi DS  Kardon RH  Grozdanic SD;CR: BioMed Vision Technologies  Neuroptics Inc 《Veterinary ophthalmology》2012,15(Z2):94-104

Objective To perform in vivo analysis of retinal functional and structural parameters in healthy mouse eyes. Animal Studied Adult C57BL/6 male mice (n?=?37). Procedures Retinal function was evaluated using pattern electroretinography (pERG) and the chromatic pupil light reflex (cPLR). Structural properties of the retina and nerve fiber layer (NFL) were evaluated using spectral-domain optical coherence tomography (SD-OCT). Results The average pERG amplitudes were found to be 11.2?±?0.7?μV (P50-N95, mean?±?SEM), with an implicit time for P50-N95 interval of 90.4?±?5.4?ms. Total retinal thickness was 229.5?±?1.7?μm (mean?±?SEM) in the area centralis region. The thickness of the retinal nerve fiber layer (mean?±?SEM) using a circular peripapillary retinal scan centered on the optic nerve was 46.7?±?0.9?μm (temporal), 46.1?±?0.9?μm (superior), 45.8?±?0.9?μm (nasal), and 48.4?±?1?μm (inferior). The baseline pupil diameter was 2.1?±?0.05?mm in darkness, and 1.1?±?0.05 and 0.56?±?0.03?mm after stimulation with red (630?nm, luminance 200?kcd/m(2) ) or blue (480?nm, luminance 200?kcd/m(2) ) light illumination, respectively. Conclusions Pattern electroretinography, cPLR and SD-OCT analysis are reproducible techniques, which can provide important information about retinal and optic nerve function and structure in mice.  相似文献   

Congenital portosystemic shunts in Maltese and Australian Cattle Dogs   总被引：2，自引：0，他引：2  

PLC TISDALL  GB HUNT ‡  CR BELLENGER †  R. MALIK † 《Australian veterinary journal》1994,71(6):174-178

SUMMARY Congenital portosystemic shunts were definitively diagnosed in 62 dogs over a period of 15 years. Maltese and Australian Cattle Dogs were significantly over-represented, accounting for 14 and 13 cases, respectively. Maltese invariably had a single extrahepatic shunt derived from the left gastric or gastrosplenic vein, whereas Cattle Dogs usually had large intrahepatic shunts involving the right liver lobes. The clinical syndromes resulting from anomalous portosystemic communications were indistinguishable in the 2 breeds. Fasting blood ammonia concentration was elevated in 20 of 22 dogs tested, providing a minimally invasive and effective means of diagnosis. Complete or partial shunt attenuation was performed successfully in all 9 Maltese and in 2 of 6 Cattle Dogs in which it was attempted.  相似文献   

响应面法优化姬松茸液体发酵培养基中红糖和酵母膏浓度   总被引：6，自引：0，他引：6  

范雷法  潘慧娟  Soccol CR  Pandey A 《食用菌学报》2005,12(3):27-31

运用响应面分析法优化姬松茸液体发酵培养基中红糖和酵母膏浓度.结果表明,红糖浓度为20.24g/L、酵母膏浓度为2.055g/L时,菌丝体生物量最高,达2.279g/L;红糖浓度为20.8g/L、酵母膏浓度为2.23g/L时,胞外多糖产量最高,达0.749g/L.  相似文献   

仔猪腹泻病的鉴别诊断及综合防治     

孔爱云  赵新  庞传文  刘万成  解爱英  曹翠荣 《中国兽药杂志》2005,39(3):48-50

对仔猪腹泻病进行了摸索、研究和总结,根据不同日龄和不同临床表现进行鉴别诊断,同时可采取组织病理学、微生物学方法进一步确诊,并采取针对性防治措施,才能有效地控制仔猪腹泻病.  相似文献   

Ampakine CX1942 attenuates opioid‐induced respiratory depression and corrects the hypoxaemic effects of etorphine in immobilized goats (Capra hircus)          下载免费PDF全文

Anna J Haw  Leith CR Meyer  John J Greer  Andrea Fuller 《Veterinary anaesthesia and analgesia》2016,43(5):528-538

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Faecal PCR panel results and clinical findings in Western Australian dogs with diarrhoea     

MW Kim  CR Sharp  CJ Boyd  LN Twomey 《Australian veterinary journal》2020,98(11):563-569

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Differential Rotation and Dynamics of the Solar Interior     

MJ Thompson  J Toomre  ER Anderson  HM Antia  G Berthomieu  D Burtonclay  SM Chitre  J Christensen-Dalsgaard  T Corbard  M DeRosa  CR Genovese  DO Gough  DA Haber  JW Harvey  F Hill  R Howe  SG Korzennik  AG Kosovichev  JW Leibacher  FP Pijpers  J Provost  EJ Rhodes  J Schou  T Sekii  PB Stark  PR Wilson 《Science (New York, N.Y.)》1996,272(5266):1300-1305

Splitting of the sun's global oscillation frequencies by large-scale flows can be used to investigate how rotation varies with radius and latitude within the solar interior. The nearly uninterrupted observations by the Global Oscillation Network Group (GONG) yield oscillation power spectra with high duty cycles and high signal-to-noise ratios. Frequency splittings derived from GONG observations confirm that the variation of rotation rate with latitude seen at the surface carries through much of the convection zone, at the base of which is an adjustment layer leading to latitudinally independent rotation at greater depths. A distinctive shear layer just below the surface is discernible at low to mid-latitudes.  相似文献   

Experimental Leptospira borgpetersenii serovar hardjo infection of pregnant cattle     

CR SMITH  MR McGOWAN  CS McCLINTOCK  BG CORNEY  PJ KETTERER  L. SMYTHE  W. WARD 《Australian veterinary journal》1997,75(11):822-826

Objective To observe the effect upon the foetus of experimental infection of pregnant cattle with Leptospira borg-petersenii serovar hardjo .
Design A disease transmission study using pregnant cattle.
Procedure Fourteen heifers serologically negative to L hardjo were artificially inseminated and later challenged with a north-Queensland isolate of L hardjo by conjunctival inoculation. The heifers were serologically monitored and their urine examined for the presence of leptospires using culture and fluorescent-antibody tests at appropriate intervals. Elective caesarean sections were performed on pregnant heifers at 6.5 weeks after the challenge. Foetuses were examined using serological, histopathological, microbiological and fluorescent-antibody tests.
Results Ten of the heifers became pregnant, but three subsequently aborted before challenge. After challenge, all 14 heifers seroconverted and L hardjo was isolated from the urine of 6 of the 7 pregnant heifers. No evidence of foetal L hardjo infection was detected. Two of the foetuses had histopatho-logical lesions consistent with Neospora s p infection.
Conclusion It is likely that the isolate of L hardjo used in this study does not normally infect the foetus. Neospora s p may be a more significant cause of bovine reproductive wastage.  相似文献   

Wounds of the pastern and foot region managed with phalangeal casts: 50 cases in 49 horses (1995–2006)     

KM Ketzner  AA Stewart  CR Byron  M Stewart  EM Gaughan  PD Vanharreveld  JD Lillich 《Australian veterinary journal》2009,87(9):363-368

Objective   Describe the use of a phalangeal cast as treatment for wounds in the pastern and foot region of horses. Secondly, to evaluate the healing and soundness of horses treated with phalangeal casts.
Design   Retrospective study of 49 horses.
Procedures   Medical records of 49 horses that were treated with a phalangeal cast for 50 cases of wounds in the pastern and foot region at equine referral hospitals from 1995 to 2006 were reviewed and follow-up information was obtained.
Results   Treatment consisted of wound debridement, lavage, wound closure (28 wounds), cast application and antibiotics (84%). At follow-up, the majority of horses were sound (42 of 47 wounds, 89.4%), three horses were still lame and one horse was euthanased because of persistent lameness. Three horses were lost to follow-up. There was no statistical difference between the outcomes of horses treated acutely (<24 h) or after a 24-h delay. Similarly, the involvement of synovial structures in the wound did not significantly influence outcome.
Conclusions   In this study, wounds involving the pastern and foot that were treated with a phalangeal cast carried a good prognosis for soundness (89.4%) and cosmetic healing (89.5%). The phalangeal casts were well-tolerated and effective.  相似文献