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MR Luz CC de Holanda JJ Pereira PMC Freitas AEP Salgado J Di Giorgio Giannotti SB de Oliveira NS Teixeira CR de Freitas Guaitolini 《Reproduction in domestic animals》2011,46(4):724-727
The embryonic collection techniques in dogs present a vast methodological variation and low recovery rates. The objectives were to compare and describe two techniques as to the recovery of canine embryos, on the 12th day after the first mating or artificial insemination. Embryos were recovered through uterine horn flushing in vivo, before performing the ovariohysterectomy (OHE) (Group 1; n = 9) or ex vivo, immediately after the OHE (Group 2; n = 9). In total, 43 and 47 embryonic structures were recovered in Groups 1 and 2, respectively. There was no significant difference (p > 0.05) between groups on recovery rates (72.8% and 81.0%, respectively). We inferred that both in vivo and ex vivo techniques allow a high rate of embryonic recovery; in the collection technique prior to the OHE, it is essential to carefully handle the reproductive system during the trans‐surgical period and that the 12th day (D12) after the first mating/artificial insemination is an efficient option for the high recovery rate of morulae and blastocysts. 相似文献
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A Chiamenti CR Aguiar Filho LM Freitas Neto RM Chaves FF Paula‐Lopes PF Lima PBD Gonçalves MAL Oliveira 《Reproduction in domestic animals》2010,45(5):e68-e72
The objective of this study was to evaluate the effect of retinol (RT) and retinoic acid (RA) on the in vitro development of pre‐implantation goat embryos cultured in potassium simplex optimized medium or synthetic oviduct fluid or cocultured in oviductal cells monolayer either in potassium simplex optimized medium or synthetic oviduct fluid. A total of 2407 cumulus‐oocyte complexes were aspirated from 2 to 6 mm ovarian follicles from slaughtered animals. Selected cumulus‐oocyte complexes were subjected to in vitro maturation in TCM 199 for 24 h at 39°C in an atmosphere of 5% (v/v) CO2 in humidified air. In vitro fertilization was performed in modified defined medium. Eighteen hours after in vitro fertilization, cumulus cells were removed and presumptive zygotes were randomly distributed into experimental groups. In Experiment 1, presumptive zygotes were cultured in potassium simplex optimized medium, potassium simplex optimized medium + RT, potassium simplex optimized medium + retinoic acid, synthetic oviduct fluid, synthetic oviduct fluid + RT and synthetic oviduct fluid + RA at 39°C in a humidified atmosphere of 5% (v/v) CO2, 5% (v/v) O2 and 90% (v/v) N2. In Experiment 2, presumptive zygotes were cocultured in potassium simplex optimized medium + oviductal cells monolayer, potassium simplex optimized medium + RT + oviductal cells monolayer, potassium simplex optimized medium + RA + oviductal cells monolayer, synthetic oviduct fluid + oviductal cells monolayer, synthetic oviduct fluid + RT + oviductal cells monolayer and synthetic oviduct fluid + RA + oviductal cells monolayer in an atmosphere of 5% (v/v) CO2 in humidified air. In both experiments, media were partially changed on day 2 after in vitro fertilization and unfertilized oocytes were excluded from the experiment. Embryos were cultured or cocultured for 8 days. In Experiment 1, there was no effect of RT or RA supplementation on the proportion of oocytes that reached the morula or blastocyst stages. By contrast, Experiment 2 demonstrated that the addition of 0.28 μg/ml RT and 0.5 μm RA to the embryo culture media stimulated (p < 0.05) development to the morula and blastocyst stages under the coculture conditions tested. In conclusion, retinoids play an important role in pre‐implantation development of goat embryos and can be used to enhance in vitro embryo production. 相似文献
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Mohan K Harper MM Kecova H Ye EA Lazic T Sakaguchi DS Kardon RH Grozdanic SD;CR: BioMed Vision Technologies Neuroptics Inc 《Veterinary ophthalmology》2012,15(Z2):94-104
Objective To perform in vivo analysis of retinal functional and structural parameters in healthy mouse eyes. Animal Studied Adult C57BL/6 male mice (n?=?37). Procedures Retinal function was evaluated using pattern electroretinography (pERG) and the chromatic pupil light reflex (cPLR). Structural properties of the retina and nerve fiber layer (NFL) were evaluated using spectral-domain optical coherence tomography (SD-OCT). Results The average pERG amplitudes were found to be 11.2?±?0.7?μV (P50-N95, mean?±?SEM), with an implicit time for P50-N95 interval of 90.4?±?5.4?ms. Total retinal thickness was 229.5?±?1.7?μm (mean?±?SEM) in the area centralis region. The thickness of the retinal nerve fiber layer (mean?±?SEM) using a circular peripapillary retinal scan centered on the optic nerve was 46.7?±?0.9?μm (temporal), 46.1?±?0.9?μm (superior), 45.8?±?0.9?μm (nasal), and 48.4?±?1?μm (inferior). The baseline pupil diameter was 2.1?±?0.05?mm in darkness, and 1.1?±?0.05 and 0.56?±?0.03?mm after stimulation with red (630?nm, luminance 200?kcd/m(2) ) or blue (480?nm, luminance 200?kcd/m(2) ) light illumination, respectively. Conclusions Pattern electroretinography, cPLR and SD-OCT analysis are reproducible techniques, which can provide important information about retinal and optic nerve function and structure in mice. 相似文献
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SUMMARY Congenital portosystemic shunts were definitively diagnosed in 62 dogs over a period of 15 years. Maltese and Australian Cattle Dogs were significantly over-represented, accounting for 14 and 13 cases, respectively. Maltese invariably had a single extrahepatic shunt derived from the left gastric or gastrosplenic vein, whereas Cattle Dogs usually had large intrahepatic shunts involving the right liver lobes. The clinical syndromes resulting from anomalous portosystemic communications were indistinguishable in the 2 breeds. Fasting blood ammonia concentration was elevated in 20 of 22 dogs tested, providing a minimally invasive and effective means of diagnosis. Complete or partial shunt attenuation was performed successfully in all 9 Maltese and in 2 of 6 Cattle Dogs in which it was attempted. 相似文献
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