Evaluation of the conjunctival swab for canine visceral leishmaniasis diagnosis by PCR-hybridization in Minas Gerais State, Brazil     

Ferreira Sde A  Ituassu LT  de Melo MN  de Andrade AS 《Veterinary parasitology》2008,155(3-4):257-263

Larvae of Oestrus ovis (Diptera: Oestridae) are ubiquitous parasites of nasal and sinusal cavities of sheep and goats. According to the chronobiology of O. ovis infections in Sardinia and the seasonal pattern of the IgG response, the optimal period to investigate the relationships between O. ovis larval populations and intensity of local and systemic IgG antibody responses was mid-July in the summer season. Sarda x Lacaune ewes (n=186), divided into three ram-families were used in the study. Systemic and local IgG responses were measured by ELISA tests using second stage larval crude extracts (L2CE) and L2 (L2SGC) and L3 (L3SGC) salivary gland contents as coating antigens. The number of larval instars, larval length of L1, L2 and L3 larvae, and larval weight of L2 and L3 larvae were individually recorded after ewe necropsy. Negative correlations among larval establishment and/or larval development on the one hand and intensity of local or systemic IgG responses on the other hand were found in two out of three studied ram-families.  相似文献   

High level of B19 strain detection in Brazilian cattle semen     

Lourencetti  M. P. S.  Souza  M. A.  Ganda  M. R.  Santos  J. P.  Ferreira Júnior  A.  Miyashiro  S.  Lima  A. M. C. 《Tropical animal health and production》2018,50(2):433-439

Tropical Animal Health and Production - Semen contaminated with microorganisms can disseminate serious diseases including brucellosis. The objectives of this study were to detect Brucella-specific...  相似文献   

Levels of supplementation of inorganic selenium and vitamin E for meat quail aged 0 to 14 and 14 to 35 days          下载免费PDF全文

V. Zancanela  A. C. Furlan  P. C. Pozza  S. M. Marcato  D. O. Grieser  C. E. Stanquevis  E. M. Finco  T. M. Oliveira‐Bruxel  M. F. Z. Ferreira 《Journal of animal physiology and animal nutrition》2018,102(2):e918-e930

Two experiments were carried out to determine the levels of supplementation of inorganic selenium (Se) and vitamin E (VE ) in diets of quails aged 0–14 and 14–35 days old. A completely randomized design was used in a factorial design (Se = 0.1125; 0.2250; 0.3375 and 0.4500 mg kg^?1 diet^?1 × VE  = 10; 23; 36 and 49 IU  kg^?1 diet^?1). In experiment 1, quail (n  = 2,400) were aged 0–14 days and were divided into 16 treatments, with three replicates of 50 birds. In experiment 2, quail (n  = 1,680) were aged 14–35 days and were divided into the same treatments, with three replicates of 35 birds. At age 0–14 days, the levels of VE did not affect performance (p  > .05); however, the feed conversion (FC ) was influenced by a quadratic effect (p  = .0515), according to the level of Se, with a higher level estimated at 0.29 mg Se kg^?1 diet^?1. At age 14–35 days, there was a linear effect with interaction (Se × VE ), for FC (p  = .0150) and weight gain (WG ; p  = .0266). FC (Se, p  = .0048 and VE , p  = .0019) and WG (Se, p  = .0049 and VE , p  = .0068) improved linearly with increasing levels of Se and VE . The feed intake (FI ) decreased linearly (p  = .0582) as a function of VE . The carcass yield showed a quadratic effect (p  = .0056) on the levels of VE , with a higher yield estimation of 27.24 IU VE /kg of diet. It can be concluded that the optimum level of supplementation at age 0–14 days was 0.29 mg Se kg^?1 diet^?1 and 10 IU VE  kg^?1 diet^?1 and at age 14–35 days, it was 0.4500 mg Se kg^?1 diet^?1 and 49 IU of VE  kg^?1 diet^?1.  相似文献   

Natriuretic peptide system regulation in granulosa cells during follicle deviation and ovulation in cattle          下载免费PDF全文

JG Ferst  JE Nóbrega Jr  PRA Rosa  MT Rovani  GF Ilha  RC Bohrer  R Ferreira  BG Gasperin  V Bordignon  PBD Gonçalves 《Reproduction in domestic animals》2018,53(3):710-717

Natriuretic peptides (NPs) are known to regulate reproductive events in polyovulatory species, but their function and regulation in monovulatory species remain to be fully characterized. Using a well‐established in vivo model, we found that bovine granulosa cells from follicles near the deviation stage express mRNA for the three NP receptors (NPR1, NPR2 and NPR3), but not for NP precursors (NPPA, NPPB and NPPC). The abundance of NPR3 mRNA was higher in dominant compared to subordinate follicles at the expected time of follicular deviation. After deviation, mRNA for all NP receptors was significantly more abundant in the dominant follicle. Intrafollicular inhibition of oestrogen receptors downregulated NPR1 mRNA in dominant follicles. In granulosa cells from preovulatory follicles, NPPC mRNA increased at 3 and 6 h after systemic GnRH treatment, but decreased at 12 and 24 h to similar levels observed in samples collected at 0 h. After GnRH treatment, NPR1 mRNA was upregulated at 24 h, NPR3 mRNA gradually decreased after 3 h, while NPR2 mRNA was not regulated. The mRNA expression of the enzyme FURIN increased at 24 h after GnRH treatment. These findings revealed that the expression of mRNA encoding important components of the NP system is regulated in bovine granulosa cells during follicular deviation and in response to GnRH treatment, which suggests a role of NP system in the modulation of these processes in monovulatory species.  相似文献   

Thermal decomposition,chemical composition,in vitro digestibility and gas production and in situ degradability of oilseed residues from the biofuel industry          下载免费PDF全文

Anderson Dias Vieira de Souza  Luís Carlos Vinhas Ítavo  Simone Palma Fávaro  Camila Celeste Brandão Ferreira Ítavo  Hélène Veronique Petit  Alexandre Menezes Dias  Maria da Graça Morais  Fernando Alvarenga Reis  Renato Roscoe 《Animal Science Journal》2018,89(1):79-87

Thermal analysis could rapidly and easily predict nutritional value of ruminant feeds. The hypothesis is that crambe meal (CM) has a quality similar to that of soybean meal (SM), and the objective of this study was to determine the nutritional characteristics of CM and compare them to those of SM. CM had greater concentrations of phytic acid (26.3 vs. 16.0 g/kg) and phenol compounds (615 vs. 393 mg gallic acid (GAE)/kg) than SM. In vitro dry matter (DM) digestibility was lower for CM than SM (752 vs. 975 g/kg DM). Cumulative in vitro gas production at 48 h of incubation (14.1 vs. 19.4 mL/100 mg substrate DM), and energy release (4.5 vs. 5.7 kJ/g substrate DM) were lower for CM than SM. CM had a higher concentration of low digestible fiber, hence degradability of DM was lower and the proportion of indigestible fraction was greater for CM than SM. High concentrations of indigestible compounds were likely responsible for lower gas production of CM compared to SM. These results suggest CM quality is lower than that of SM and that thermal analysis is a useful tool to precisely determine the nutritive value of oilseed residues.  相似文献   

Luteal vascularity and embryo dynamics in mares during early gestation: Effect of age and endometrial degeneration     

Jair Camargo Ferreira  Yatta Linhares Boakari  Noeme Sousa Rocha  Fernanda Saules Igncio  Guilherme Barbosa da Costa  Cezinande de Meira 《Reproduction in domestic animals》2019,54(3):571-579

The present study characterized the luteal status and the dynamic of the conceptus during the first 20 days of gestation in mares with different ages and degrees of endometrial degeneration. Total area of the corpus luteum (CL), luteal vascularity, CL area with blood signals, progesterone concentrations (P4), embryonic vesicle diameter, number of embryonic location changes, embryonic fixation position and uterine contractility were evaluated. In Experiment 1, mares ≤6 years of age (Young group, 5.6 ± 0.2 years, n = 7 mares) and mares ≥15 years of age (Old group, 17.2 ± 0.9 years, n = 6 mares) were used to investigate the effect of age. In Experiment 2, the luteal and embryonic parameters were compared between mares with minimal (Mild group, endometrial category I, n = 9 mares) and severe (Severe group, endometrial category III, n = 7 mares) endometrial degeneration. The Old and Severe groups had greater (p ≤ 0.04) total CL area and reduced luteal vascularity (p ≤ 0.04) than the Young and Mild groups, respectively. However, P4 levels and CL area with blood signals were similar (p ≥ 0.8) between the groups. A negative effect of age (p < 0.01), but not of endometrial degeneration (p = 0.6), was found for the embryonic vesicle diameter. The conceptus mobility was high (p > 0.1) until day 14 of gestation in the Severe group, while a reduced number of changes of the embryo location was detected earlier (p < 0.05) in the Old group. In conclusion, the newly formed CL of aged mares and mares with severe endometrial degeneration suffered a structural remodelling to safeguard the local blood supply and the continuous P4 output during early gestation. Moreover, an earlier reduction of the embryonic mobility and a delayed development of the conceptus were associated with advanced age, regardless of the degree of endometrial degeneration.  相似文献   

The dynamics of gene expression,lipid composition and DNA methylation reprogramming are different during in vitro maturation of pig oocytes obtained from prepubertal gilts and cycling sows     

Thiago Felipe Braga  Thainara Christie Ferreira Silva  Mariana Groke Marques  Andressa Pereira de Souza  Daniela Albring  Luciano Paulino Silva  Alexandre Rodrigues Caetano  Margot Alves Nunes Dode  Maurício Machaim Franco 《Reproduction in domestic animals》2019,54(9):1217-1229

  相似文献   

Detection of Toxoplasma gondii in Crassostrea spp. oysters cultured in an estuarine region in eastern Amazon     

Thamillys R. M. Monteiro  Katarine S. Rocha  Jacqueline Silva  Gleiciane S. S. Mesquita  Marcely K. S. Rosrio  Maeli F. S. Ferreira  Betsy E. T. Honorio  Hugo F. R. Melo  Flvia N. L. Barros  Alessandra Scofield  Isis Abel  Carla C. G. Moraes 《Zoonoses and public health》2019,66(3):296-300

The aim of the present study was to detect DNA of Toxoplasma gondii in Crassostrea spp. oysters cultured in the state of Pará, Brazil. A total of 400 oysters were directly collected from a fixed rack system. Gills, gastrointestinal tract (GIT) and intervalvular liquid were separated and grouped into pool samples of 10 animals, resulting in 40 samples each of gills, GIT and intervalvular liquid. DNA extraction was performed using a commercial kit, and T. gondii DNA was detected by nested PCR using the primers Toxo3 and Toxo4, which produced an amplification product of 155 bp of the T. gondii gene B1. Nucleotide sequencing was performed for positive samples, and the obtained sequences were identified by comparison with sequences in GenBank. The DNA of T. gondii was detected in 5.8% (7/120) of the pool samples, of which 7.5% (3/40) was in the GIT, 5% (2/40) in the gills, and 5% (2/40) was in the intervalvular liquid. The obtained sequences presented 100% identity and overlap with T. gondii DNA sequences. This is the report of detection of T. gondii DNA in oysters from genus Crassostrea spp. originating from the state of Pará, eastern Amazon.  相似文献   

Determination of creatinine excretion and evaluation of spot urine sampling in Holstein cattle   总被引：1，自引：0，他引：1  

Mario Luiz Chizzotti  Sebastio de Campos Valadares Filho  Rilene Ferreira Diniz Valadares  Fernanda Helena Martins Chizzotti  Luis Orlindo Tedeschi 《Livestock Science》2008,113(2-3):218-225

Four experiments were carried out to determine urinary creatinine excretion in Holstein growing bulls, lactating cows, and replacement heifers. In addition, we evaluated the use of spot sampling technique to estimate purine derivatives (PD) excretion. In Experiment I, 15 lactating cows were used in a randomized block design to compare creatinine excretion obtained in different time-spans of urine collection (during 6, 9, 12, 15, 18, 21, and 24 h). In Experiment II, four bulls were allocated in a 4 × 4 Latin square to evaluate the effect of diet (levels of cottonseed hulls of 0, 10, 20, and 30% of the DM) on excretion of creatinine. In Experiment III, 15 lactating cows were used to evaluate the effect of milk production (ranging from 3.9 to 36.7 kg/d) on daily creatinine and PD excretions. In Experiment IV, 22 replacement heifers were utilized to evaluate the effect of body weight (BW, ranging from 107 to 545 kg) on daily creatinine and PD excretions. For all experiments, total urine collections were made over 24 h and daily creatinine and PD excretions were determined. Different time-spans for total urine collection had no effect (P = 0.70) on creatinine excretion compared to the 24-h collection period, indicating a constant excretion rate of creatinine. The roughage source did not influence (P = 0.64) creatinine excretion by bulls, averaging 0.248 ± 0.008 mmol/kg BW. Similarly, milk production did not affect (P = 0.82) creatinine excretion in cows, averaging 0.212 ± 0.004 mmol/kg BW. In contrast, the creatinine excretion (mmol/kg BW) decreased linearly (P < 0.001) as BW of heifers increased, suggesting that creatinine excretion might vary with the degree of maturity of growing animals. There were no differences (P > 0.14) between the 24-h total collection and spot sampling technique in estimating daily PD excretion. The spot sampling technique may be used to estimate the daily excretion of urinary PD in Holstein cattle under practical conditions.  相似文献   

Traditional knowledge, genetic and morphological diversity in populations of Spondias tuberosa Arruda (Anacardiaceae)     

Ernani Machado de Freitas Lins Neto  Iêda Ferreira de Oliveira  Fabio Barros Britto  Ulysses Paulino de Albuquerque 《Genetic Resources and Crop Evolution》2013,60(4):1389-1406

Plant domestication is an evolutionary process whose artificial selection and management change the landscape, modeling plants to satisfy the human needs, affecting the phenotype component(s) of the species under manipulation. This study aimed at examining and comparing two rural communities: Malhada de Areia e Carão, municipally of Altinho, Pernambuco regarding the knowledge, perception of morphological variations (size fruit, flavor fruit, shape fruit, yield pulp and fruit color), morphological characteristics of preference at the time of collection; evaluation on the inter- and intrapopulation morphological differences of species and assessment of diversity, variability and local genetic structure of Spondias tuberosa populations under different management regimes based on the ISSR analysis. The results pointed to the maintenance of local genetic and morphological diversity, being it strongly related to the management practices of the species, especially the S. tuberosa tolerance in open areas for farming and pasture. Thus, it is emphasized the importance of this study in Brazil by being the first time that aspects related to management and perceived diversity, morphology and genetics of a highlighted perennial species from the semi-arid region of northeastern Brazil are evaluated from the perspective of domestication studies on its incipient status. Moreover, it provides interesting data that will contribute to the conservation strategies and breeding programs.  相似文献