Detection of Toxoplasma gondii in Crassostrea spp. oysters cultured in an estuarine region in eastern Amazon     

Thamillys R. M. Monteiro  Katarine S. Rocha  Jacqueline Silva  Gleiciane S. S. Mesquita  Marcely K. S. Rosrio  Maeli F. S. Ferreira  Betsy E. T. Honorio  Hugo F. R. Melo  Flvia N. L. Barros  Alessandra Scofield  Isis Abel  Carla C. G. Moraes 《Zoonoses and public health》2019,66(3):296-300

The aim of the present study was to detect DNA of Toxoplasma gondii in Crassostrea spp. oysters cultured in the state of Pará, Brazil. A total of 400 oysters were directly collected from a fixed rack system. Gills, gastrointestinal tract (GIT) and intervalvular liquid were separated and grouped into pool samples of 10 animals, resulting in 40 samples each of gills, GIT and intervalvular liquid. DNA extraction was performed using a commercial kit, and T. gondii DNA was detected by nested PCR using the primers Toxo3 and Toxo4, which produced an amplification product of 155 bp of the T. gondii gene B1. Nucleotide sequencing was performed for positive samples, and the obtained sequences were identified by comparison with sequences in GenBank. The DNA of T. gondii was detected in 5.8% (7/120) of the pool samples, of which 7.5% (3/40) was in the GIT, 5% (2/40) in the gills, and 5% (2/40) was in the intervalvular liquid. The obtained sequences presented 100% identity and overlap with T. gondii DNA sequences. This is the report of detection of T. gondii DNA in oysters from genus Crassostrea spp. originating from the state of Pará, eastern Amazon.  相似文献   

The lesser anteater (Tamandua tetradactyla) is not tetradactyla,but pentadactyla     

Ana Cecília Machado Cruvinel  Matheus Camargos de Britto Rosa  Bruna Lívia Lopes Guimares  Cecília Ferreira Amaral Silva  Paulo Henrique de Sampaio da Silva  Cesar de Lima Borges Leo  Juliano V. Peixoto  Joo Chrysostomo de Resende Júnior  Antonio Carlos Cunha Lacreta Junior  Marcelo Passamani  Andr Rodrigues da Cunha Barreto‐Vianna  Eduardo M. M. de Lima  Gregrio Corrêa Guimares 《Anatomia, histologia, embryologia》2019,48(3):256-263

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Luteal vascularity and embryo dynamics in mares during early gestation: Effect of age and endometrial degeneration     

Jair Camargo Ferreira  Yatta Linhares Boakari  Noeme Sousa Rocha  Fernanda Saules Igncio  Guilherme Barbosa da Costa  Cezinande de Meira 《Reproduction in domestic animals》2019,54(3):571-579

The present study characterized the luteal status and the dynamic of the conceptus during the first 20 days of gestation in mares with different ages and degrees of endometrial degeneration. Total area of the corpus luteum (CL), luteal vascularity, CL area with blood signals, progesterone concentrations (P4), embryonic vesicle diameter, number of embryonic location changes, embryonic fixation position and uterine contractility were evaluated. In Experiment 1, mares ≤6 years of age (Young group, 5.6 ± 0.2 years, n = 7 mares) and mares ≥15 years of age (Old group, 17.2 ± 0.9 years, n = 6 mares) were used to investigate the effect of age. In Experiment 2, the luteal and embryonic parameters were compared between mares with minimal (Mild group, endometrial category I, n = 9 mares) and severe (Severe group, endometrial category III, n = 7 mares) endometrial degeneration. The Old and Severe groups had greater (p ≤ 0.04) total CL area and reduced luteal vascularity (p ≤ 0.04) than the Young and Mild groups, respectively. However, P4 levels and CL area with blood signals were similar (p ≥ 0.8) between the groups. A negative effect of age (p < 0.01), but not of endometrial degeneration (p = 0.6), was found for the embryonic vesicle diameter. The conceptus mobility was high (p > 0.1) until day 14 of gestation in the Severe group, while a reduced number of changes of the embryo location was detected earlier (p < 0.05) in the Old group. In conclusion, the newly formed CL of aged mares and mares with severe endometrial degeneration suffered a structural remodelling to safeguard the local blood supply and the continuous P4 output during early gestation. Moreover, an earlier reduction of the embryonic mobility and a delayed development of the conceptus were associated with advanced age, regardless of the degree of endometrial degeneration.  相似文献   

The dynamics of gene expression,lipid composition and DNA methylation reprogramming are different during in vitro maturation of pig oocytes obtained from prepubertal gilts and cycling sows     

Thiago Felipe Braga  Thainara Christie Ferreira Silva  Mariana Groke Marques  Andressa Pereira de Souza  Daniela Albring  Luciano Paulino Silva  Alexandre Rodrigues Caetano  Margot Alves Nunes Dode  Maurício Machaim Franco 《Reproduction in domestic animals》2019,54(9):1217-1229

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Determination of creatinine excretion and evaluation of spot urine sampling in Holstein cattle   总被引：1，自引：0，他引：1  

Mario Luiz Chizzotti  Sebastio de Campos Valadares Filho  Rilene Ferreira Diniz Valadares  Fernanda Helena Martins Chizzotti  Luis Orlindo Tedeschi 《Livestock Science》2008,113(2-3):218-225

Four experiments were carried out to determine urinary creatinine excretion in Holstein growing bulls, lactating cows, and replacement heifers. In addition, we evaluated the use of spot sampling technique to estimate purine derivatives (PD) excretion. In Experiment I, 15 lactating cows were used in a randomized block design to compare creatinine excretion obtained in different time-spans of urine collection (during 6, 9, 12, 15, 18, 21, and 24 h). In Experiment II, four bulls were allocated in a 4 × 4 Latin square to evaluate the effect of diet (levels of cottonseed hulls of 0, 10, 20, and 30% of the DM) on excretion of creatinine. In Experiment III, 15 lactating cows were used to evaluate the effect of milk production (ranging from 3.9 to 36.7 kg/d) on daily creatinine and PD excretions. In Experiment IV, 22 replacement heifers were utilized to evaluate the effect of body weight (BW, ranging from 107 to 545 kg) on daily creatinine and PD excretions. For all experiments, total urine collections were made over 24 h and daily creatinine and PD excretions were determined. Different time-spans for total urine collection had no effect (P = 0.70) on creatinine excretion compared to the 24-h collection period, indicating a constant excretion rate of creatinine. The roughage source did not influence (P = 0.64) creatinine excretion by bulls, averaging 0.248 ± 0.008 mmol/kg BW. Similarly, milk production did not affect (P = 0.82) creatinine excretion in cows, averaging 0.212 ± 0.004 mmol/kg BW. In contrast, the creatinine excretion (mmol/kg BW) decreased linearly (P < 0.001) as BW of heifers increased, suggesting that creatinine excretion might vary with the degree of maturity of growing animals. There were no differences (P > 0.14) between the 24-h total collection and spot sampling technique in estimating daily PD excretion. The spot sampling technique may be used to estimate the daily excretion of urinary PD in Holstein cattle under practical conditions.  相似文献   

Regulation of Anti‐Müllerian Hormone and Its Receptor Expression around Follicle Deviation in Cattle          下载免费PDF全文

GF Ilha  MT Rovani  BG Gasperin  R Ferreira  MP de Macedo  OA Neto  R Duggavathi  V Bordignon  PBD Gonçalves 《Reproduction in domestic animals》2016,51(2):188-194

The anti‐Müllerian hormone (AMH) is an important marker of ovarian reserve and for predicting the response to superovulatory treatments in several species. The objective of this study was to investigate whether AMH and its receptor (AMHR2) are regulated in bovine granulosa cells during follicular development. In the first experiment, granulosa cells were retrieved from the two largest follicles on days 2 (before), 3 (at the expected time) or 4 (after deviation) of follicular wave. In the second experiment, four doses of FSH (30, 30, 20 and 20 mg) or saline were administered twice a day starting on Day 2 of the first follicular wave of the cycle. Granulosa cells and follicular fluid were collected from the two largest follicles 12 h after the last injection of FSH or saline. AMH mRNA abundance was similar in granulosa cells of the two largest follicles (F1 and F2) before deviation (Day 2), but greater in dominant (DF) than subordinate follicles (SF) at the expected time (Day 3) and after (Day 4) deviation (p < 0.05). In experiment 1, AMH mRNA levels declined in both DF and SF near the expected time and after deviation when compared to before deviation. There was no difference in AMHR2 mRNA levels before and during follicular deviation (p > 0.05), but they tended to be greater in DFs than SFs (p < 0.1) after deviation. Experiment 2 showed that AMH and AMHR2 mRNA in granulosa cells and AMH protein abundance in follicular fluid were similar (p > 0.05) between both co‐dominant follicles collected from the FSH‐treated cows. These findings indicate the followings: AMH mRNA levels decrease in both DFs and SFs during follicular deviation; granulosa cells from heathy follicles express more AMH mRNA compared to subordinate follicles undergoing atresia and FSH stimulates AMH and AMHR2 mRNA expression in granulosa cells of co‐dominant follicles.  相似文献   

Hemodynamics of the pectinis oculi artery in American pekin ducks (Anas platyrhynchos domestica)          下载免费PDF全文

Thiago Alegre C. Ferreira  Amália Turner Giannico  Fabiano Montiani‐Ferreira 《Veterinary ophthalmology》2016,19(5):409-413

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Effects of epidural administration of dexmedetomidine on the minimum alveolar concentration of isoflurane in dogs     

Campagnol D  Teixeira Neto FJ  Giordano T  Ferreira TH  Monteiro ER 《American journal of veterinary research》2007,68(12):1308-1318

Objective-To evaluate the effects of epidural administration of 3 doses of dexmedetomidine on isoflurane minimum alveolar concentration (MAC) and characterize changes in bispectral index (BIS) induced by nociceptive stimulation used for MAC determination in dogs. Animals-6 adult dogs. Procedures-Isoflurane-anesthetized dogs received physiologic saline (0.9% NaCl) solution (control treatment) or dexmedetomidine (1.5 [DEX1.5], 3.0 [DEX3], or 6.0 [DEX6] mug/kg) epidurally in a crossover study. Isoflurane MAC (determined by use of electrical nociceptive stimulation of the hind limb) was targeted to be accomplished at 2 and 4.5 hours. Changes in BIS attributable to nociceptive stimulation and cardiopulmonary data were recorded at each MAC determination. Results-With the control treatment, mean +/- SD MAC values did not change over time (1.57 +/- 0.23% and 1.55 +/- 0.25% at 2 and 4.5 hours, respectively). Compared with the control treatment, MAC was significantly lower at 2 hours (13% reduction) but not at 4.5 hours (7% reduction) in DEX1.5-treated dogs and significantly lower at 2 hours (29% reduction) and 4.5 hours (13% reduction) in DEX3-treated dogs. The DEX6 treatment yielded the greatest MAC reduction (31% and 22% at 2 and 4.5 hours, respectively). During all treatments, noxious stimulation increased BIS; but changes in BIS were correlated with increases in electromyographic activity. Conclusions and Clinical Relevance-In dogs, epidural administration of dexmedetomidine resulted in dose-dependent decreases in isoflurane MAC and that effect decreased over time. Changes in BIS during MAC determinations may not represent increased awareness because of the possible interference of electromyographic activity.  相似文献   

Orchitis in roosters with reduced fertility associated with avian infectious bronchitis virus and avian metapneumovirus infections     

Villarreal LY  Brandão PE  Chacón JL  Assayag MS  Maiorka PC  Raffi P  Saidenberg AB  Jones RC  Ferreira AJ 《Avian diseases》2007,51(4):900-904

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Molecular characterization of infectious bronchitis virus strains isolated from the enteric contents of Brazilian laying hens and broilers     

Villarreal LY  Brandão PE  Chacón JL  Saidenberg AB  Assayag MS  Jones RC  Ferreira AJ 《Avian diseases》2007,51(4):974-978

Infectious bronchitis virus (IBV) is the causative agent of avian infectious bronchitis, which is characterized by respiratory, reproductive, and renal signs. However, the role of IBV as an enteric pathogen in still controversial. In Brazil, antigenic groups of IBV divergent from the Massachusetts serotype used for vaccination schedules in that country have already been demonstrated. The present study aimed to assess the different genotypes of IBV in Brazilian commercial poultry flocks by partial sequencing of the S1 amino-terminus coding region using enteric contents as samples and examine their relationship with the vaccine serotype currently in use. Samples of enteric contents were taken as pools of five birds from each of 18 poultry farms (17 broiler and one laying farm) from five Brazilian states between 2002 and 2006. Birds were presenting watery diarrhea and poor general condition but were without respiratory, renal, or reproductive signs. Conventional antibacterial and anticoccidial therapies were unsuccessful and, furthermore, all samples proved negative for rotavirus, reovirus, and astrovirus. Eleven IBV samples were isolated in embryonated eggs and resulted in S1 sequences. Phylogenetic analysis showed that these segregated into an exclusive cluster, close to serotype D274, but distant from Massachusetts. Mean amino acid identity amongst these Brazilian strains was 94.07%; amongst these and serotypes D274, 4/91, and Massachusetts, mean amino acid identity was 77.17%, 69.94%, and 68.93%, respectively. In conclusion, the presence of genotype variant strains of IBV in Brazilian poultry flocks has been demonstrated and might be the reason for the unsuccessful control of IBV in Brazil. Furthermore, these results also strengthen the implications of IBV in enteric diseases of poultry.  相似文献