Biomarkers predictive of long-term fertility found in vaginal lipidome of gilts at weaning     

Kayla M Mills  Christina R Ferreira  Jebadiah G Stevens  Kara R Stewart  Theresa M Casey 《Journal of animal science》2021,99(8)

A marker indicative of the fertility potential of replacement gilts early in development would decrease culling rates in the sow herd, improve sow herd reproductive efficiency, and reduce production costs. The objective of this study was to determine if vaginal lipid profiles at 21 d postnatal (PN) could predict sow reproductive performance. Vaginal swabs of the anterior vagina were taken at 21 ± 4 d PN from gilts born on a commercial sow production facility for lipidomic analysis. Animals were followed prospectively for 2 yr and assigned to reproductive performance categories based on the observation of estrus or piglets weaned per sow per year (PSY) across two farrowings. Lipids were extracted from cellular material collected with swabs taken from high fertility (HF; n = 28; ≥26 PSY) and infertile (IF; n = 34; no estrus, no pregnancy) animals, and multiple reaction monitoring profiling was used for lipidome analysis. The relative abundance of arachidonic acid (C20:4) and docosahexaenoic acid (C22:6) was lower (P < 0.05) in IF gilts than HF gilts, whereas the abundance of the free fatty acids such as cerotic (C26:0), ximenic (C26:1), and nonadecanoic (C19:0) acids was greater (P < 0.05) in IF gilts. Additionally, eicosapentaenoic acid (C20:5), a precursor of prostaglandins, was higher (P < 0.05) in IF gilts. The perspective of having a panel of lipids captured with vaginal swabs at weaning that can predict the reproductive efficiency of gilts shows promise and warrants future research in this area.  相似文献   

Leaf and canopy optical characteristics as crop‐N‐status indicators for field nitrogen management in corn     

Lisandro Rambo  Bao‐Luo Ma  Youcai Xiong  Paulo Regis Ferreira da Silvia 《植物养料与土壤学杂志》2010,173(3):434-443

The primary constraint of predicting the economic optimum nitrogen rate (EONR) for corn (Zea mays L.) is the high variability of soil nitrogen (N) supply due to environments, soil types, manure, and cropping histories. Portable instruments have been developed to measure leaf and canopy optical characteristics for determining plant N status. The objectives of this field study were to: (1) evaluate leaf and canopy optical properties including transmittance, reflectance, and fluorescence as indicators of corn N status with soil types, developmental stages, and N‐application rates, (2) compare the efficiency of two commercial radiometers that are designed to measure canopy reflectance, and (3) assess the constraints of these crop‐based indicators as a possible guide for real‐time N sidedressing in corn. Field experiments with different levels of N, soil types, and corn hybrids were conducted at three sites in Ottawa, ON, Canada, in 2004 and 2005. Leaf chlorophyll concentrations (SPAD chlorophyll meter), chlorophyll fluorescence (OS‐30), leaf area, and canopy reflectance (NDVI measured by CropScan and GreenSeeker radiometers) were simultaneously measured at several growth stages, while grain yield was determined at harvest. Our results show that canopy reflectance (NDVI) displayed similar efficiency as an indicator of N status on both soil types and corn hybrids in the two consecutive years. The chlorophyll readings often differentiated N‐deficient from N‐sufficient plots and therefore were a promising indicator for predicting corn N requirements. The fluorometer device evaluated in this study was unable to characterize corn N status.  相似文献   

Humoral immune response of water buffalo monitored with three different antigens of Toxocara vitulorum     

de Souza EM  Starke-Buzetti WA  Ferreira FP  Neves MF  Machado RZ 《Veterinary parasitology》2004,122(1):67-78

Humoral immune response of water buffalo naturally infected with Toxocara vitulorum was monitored using three different antigens of this parasite in serum and colostrum of buffalo cows and calves. Soluble extract (Ex) and excretory/secretory (ES) larval antigens and perienteric fluid antigen (Pe) of adult T. vitulorum were used to measure the antibody levels by an indirect ELISA. Serum of 7-12 buffalo cows for the first 365 days and colostrum of the same number of buffalo cows for the first 60 days of parturition, and serum of 8-10 buffalo calves for the first 365 days after birth were assayed. The ELISA detected antibodies against all three T. vitulorum antigens in the colostrum and serum of 100% of buffalo cows and calves examined. The highest antibody levels against Ex, ES and Pe antigens were detected in the buffalo cow sera during the perinatal period and were maintained at high levels through 300 days after parturition. On the other hand, colostrum antibody concentrations of all three antigens were highest on the first day post-parturition, but decreased sharply during the first 15 days. Concomitantly to the monitoring of immune response, the parasitic status of the calves was also evaluated. In calves, antibodies passively acquired were at the highest concentrations 24 h after birth and remained at high levels until 45 days coincidentally with the peak of T. vitulorum infection. The rejection of the worms by the calves occurred simultaneously with the decline of antibody levels, which reached their lowest levels between 76 and 150 days. Thereafter, probably because of the presence of adults/larvae stimulation, the calves acquired active immunity and the antibodies started to increase slightly in the serum and plateaued between the days 211 and 365. All three antigens were detected by the serum antibodies of buffalo calves; however, the concentration of anti-Pe antibody was higher than anti-EX and anti-ES, particularly after 90 days of age. By conclusion, the buffalo cows develop immunity and keep high levels of antibodies against T. vitulorum-Ex, ES and Pe antigens and these antibodies are transferred to their calves through the colostrum. This passively acquired immunity does not protect the calves against the acquisition of the infection, but these antibodies, passively or actively acquired, may have an important role during worm rejection by the calves and prevention of intestinal reinfection.  相似文献   

Comparison of analgesia and perioperative problems associated with the preoperative use of ketoprofen and nimesulide in dogs     

X Ferreira  L Lambert  A Leblond  E  Troncy 《Veterinary anaesthesia and analgesia》2001,28(4):207-207

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Use of a multiplex PCR-based assay to differentiate Campylobacter jejuni and Campylobacter coli strains isolated from human and animal sources.     

M H C Aquino  A H Regua Mangia  A L L Filgueiras  L M Teixeira  M C S Ferreira  A Tibana 《Veterinary journal (London, England : 1997)》2002,163(1):102-104

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Testicular measurements in Campolina stallions     

H.C. Manso Filho  H.E.C. Costa  F.L. Santos  M.G. Abage  L.M.C. Ferreira  S.R. Marques   《Journal of Equine Veterinary Science》2000,20(4)

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In vitro Bioaccessibility of Proteins,Phenolics, Flavonoids and Antioxidant Activity of Amaranthus viridis     

Silva  Alisson David  Ávila  Suelen  Küster  Renata Tulio  dos Santos  Mayara Padovan  Grassi  Marco Tadeu  de Queiroz Pereira Pinto  Christiane  Miguel  Obdulio Gomes  Ferreira  Sila Mary Rodrigues 《Plant foods for human nutrition (Dordrecht, Netherlands)》2021,76(4):478-486

Plant Foods for Human Nutrition - The aim of this study was to investigate the chemical composition, mineral content and report the effect of gastrointestinal digestion on the proteins, phenolics,...  相似文献   

Hypoglycemic and Vasorelaxant Effect of Passiflora edulis Fruit Peel By-Product     

Cabral  Bárbara  Bortolin  Raul Hernandes  Gonçalves  Tays Amanda Felisberto  Maciel  Priscilla Maria Pereira  de Arruda  Alinne Villar  de Carvalho  Thais Gomes  Abboud  Kahlile Youssef  Alves  Jovelina Samara Ferreira  Cordeiro  Lucimara M. C.  de Medeiros  Isac Almeida  de Rezende  Adriana Augusto  Zucolotto  Silvana Maria 《Plant foods for human nutrition (Dordrecht, Netherlands)》2021,76(4):466-471

Plant Foods for Human Nutrition - Passiflora edulis fo. flavicarpa (Passifloraceae) is popularly known as yellow passion fruit and its fruit peels are considered a rich by-product in bioactive...  相似文献   

Traditional knowledge, genetic and morphological diversity in populations of Spondias tuberosa Arruda (Anacardiaceae)     

Ernani Machado de Freitas Lins Neto  Iêda Ferreira de Oliveira  Fabio Barros Britto  Ulysses Paulino de Albuquerque 《Genetic Resources and Crop Evolution》2013,60(4):1389-1406

Plant domestication is an evolutionary process whose artificial selection and management change the landscape, modeling plants to satisfy the human needs, affecting the phenotype component(s) of the species under manipulation. This study aimed at examining and comparing two rural communities: Malhada de Areia e Carão, municipally of Altinho, Pernambuco regarding the knowledge, perception of morphological variations (size fruit, flavor fruit, shape fruit, yield pulp and fruit color), morphological characteristics of preference at the time of collection; evaluation on the inter- and intrapopulation morphological differences of species and assessment of diversity, variability and local genetic structure of Spondias tuberosa populations under different management regimes based on the ISSR analysis. The results pointed to the maintenance of local genetic and morphological diversity, being it strongly related to the management practices of the species, especially the S. tuberosa tolerance in open areas for farming and pasture. Thus, it is emphasized the importance of this study in Brazil by being the first time that aspects related to management and perceived diversity, morphology and genetics of a highlighted perennial species from the semi-arid region of northeastern Brazil are evaluated from the perspective of domestication studies on its incipient status. Moreover, it provides interesting data that will contribute to the conservation strategies and breeding programs.  相似文献   

The Paleozoic origin of enzymatic lignin decomposition reconstructed from 31 fungal genomes     

Floudas D  Binder M  Riley R  Barry K  Blanchette RA  Henrissat B  Martínez AT  Otillar R  Spatafora JW  Yadav JS  Aerts A  Benoit I  Boyd A  Carlson A  Copeland A  Coutinho PM  de Vries RP  Ferreira P  Findley K  Foster B  Gaskell J  Glotzer D  Górecki P  Heitman J  Hesse C  Hori C  Igarashi K  Jurgens JA  Kallen N  Kersten P  Kohler A  Kües U  Kumar TK  Kuo A  LaButti K  Larrondo LF  Lindquist E  Ling A  Lombard V  Lucas S  Lundell T  Martin R  McLaughlin DJ  Morgenstern I  Morin E  Murat C  Nagy LG  Nolan M  Ohm RA  Patyshakuliyeva A 《Science (New York, N.Y.)》2012,336(6089):1715-1719

Wood is a major pool of organic carbon that is highly resistant to decay, owing largely to the presence of lignin. The only organisms capable of substantial lignin decay are white rot fungi in the Agaricomycetes, which also contains non-lignin-degrading brown rot and ectomycorrhizal species. Comparative analyses of 31 fungal genomes (12 generated for this study) suggest that lignin-degrading peroxidases expanded in the lineage leading to the ancestor of the Agaricomycetes, which is reconstructed as a white rot species, and then contracted in parallel lineages leading to brown rot and mycorrhizal species. Molecular clock analyses suggest that the origin of lignin degradation might have coincided with the sharp decrease in the rate of organic carbon burial around the end of the Carboniferous period.  相似文献