ASAS-NANP SYMPOSIUM: Applications of machine learning for livestock body weight prediction from digital images     

Zhuoyi Wang  Saeed Shadpour  Esther Chan  Vanessa Rotondo  Katharine M Wood  Dan Tulpan 《Journal of animal science》2021,99(2)

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Effects of dietary Bacillus coagulans and yeast hydrolysate supplementation on growth performance,immune response and intestinal barrier function in weaned piglets     

Runqi Fu  Chan Liang  Daiwen Chen  Hui Yan  Gang Tian  Ping Zheng  Jun He  Jie Yu  Xiangbing Mao  Zhiqing Huang  Yuheng Luo  Junqiu Luo  Bing Yu 《Journal of animal physiology and animal nutrition》2021,105(5):898-907

The present study investigated the effects of Bacillus coagulans and yeast hydrolysate supplementation on growth performance, immune response and intestinal barrier function of weaned piglets. Twenty-four weaned piglets with an average body weight (BW) of 6.89 ± 0.15 kg were divided into four diets for 28 days. The treatments were basal diet (control), basal diet supplemented with antibiotic (20 mg/kg colistin sulphate and 40 mg/kg bacitracin zinc, AT), probiotics (400 mg/kg Bacillus coagulans ≥5 × 109 CFU/g, BC) or yeast hydrolysate (5000 mg/kg yeast hydrolysate, YH). Average daily gain (ADG) and average daily feed intake (ADFI) were improved by AT and YH diets (p < 0.05), while BC diet only increased ADG (p < 0.05). The complement 3 (C3), lysozyme (LZM) and tumour necrosis factor-α (TNF-α) concentrations in serum were increased in BC diet (p < 0.05). Feeding AT and YH caused the increase of jejunal villus height (p < 0.05), and a higher ratio of villus height/crypt depth was observed in AT, BC and YH groups (p < 0.05). The mRNA expression of zonula occludens-1 (ZO-1) in jejunal mucosa was up-regulated by AT, BC and YH diets (p < 0.05). Dietary AT, BC or YH inclusion decreased the interleukin-1β (IL-1β) concentration and TNF-α mRNA expression (p < 0.05), and YH supplementation even down-regulated toll-like receptor 4 (TLR4) and CD14 expressions (p < 0.05). In summary, the dietary administration of BC or YH both improves growth performance through promoting the intestinal barrier function, indicating both of them can serve as potential alternatives to antibiotics growth promoters for the piglet production.  相似文献   

Thromboelastographic assessment of the contribution of platelets and clotting proteases to the hypercoagulable state of dogs with immune‐mediated hemolytic anemia          下载免费PDF全文

Negar Hamzianpour BSc  BVSc  MRCVS  Daniel L. Chan DVM  DACVECC  DECVECC  DACVN  MRCVS 《Journal of Veterinary Emergency and Critical Care》2016,26(2):295-299

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Generation of Salmonella ghost cells expressing fimbrial antigens of enterotoxigenic Escherichia coli and evaluation of their antigenicity in a murine model     

Chan Song Kim  Jin Hur  Seong Kug Eo  Sang-Youel Park  John Hwa Lee 《Canadian journal of veterinary research》2016,80(1):40-48

Salmonella Typhimurium ghost cells expressing K88ab, K88ac, K99, and FasA fimbriae of enterotoxigenic Escherichia coli (ETEC) in their envelopes were constructed. The genes encoding the fimbriae were individually cloned into an expression plasmid, pMMP81, carrying the asd gene, which was subsequently electroporated into the Δasd S. Typhimurium mutant. Plasmid pJHLP99, carrying the phiX174 lysis gene E, was also subsequently electroporated into the Salmonella mutant. The presence of the individual fimbriae on the ghost cells was examined by Western blot analysis. Forty BALB/c mice were equally divided into 2 groups of 20 mice each. Group A mice were intramuscularly vaccinated with a mixture of the 4 ghost cells expressing the individual fimbriae. The group B mice were inoculated with sterile phosphate-buffered saline as a control. The antigen-specific serum IgG concentrations were significantly higher in group A than in group B from week 2 until week 6 after inoculation. In addition, the antigen-specific IgA concentrations in fecal samples were significantly higher in group A than in group B at week 2 after inoculation. A large difference between the groups in the number of antigen-specific IgA-secreting cells in the small intestine was observed by immunohistochemical study. Also, the splenic lymphocyte proliferative responses were significantly greater in group A than in the control mice. These results suggest that vaccination with our Salmonella ghost cells can induce both humoral and cell-mediated immune responses and that the increased number of antigen-specific IgA-secreting cells in the small intestine may be correlated with the elevated fecal IgA immune response.  相似文献   

Pyruvate kinase is necessary for Brucella abortus full virulence in BALB/c mouse     

Jianpeng Gao  Mingxing Tian  Yanqing Bao  Peng Li  Jiameng Liu  Chan Ding  Shaohui Wang  Tao Li  Shengqing Yu 《Veterinary research》2016,47(1)

Brucellosis, caused by a facultative intracellular pathogen Brucella, is one of the most prevalent zoonosis worldwide. Host infection relies on several uncanonical virulence factors. A recent research hotpot is the links between carbon metabolism and bacterial virulence. In this study, we found that a carbon metabolism-related pyruvate kinase (Pyk) encoded by pyk gene (locus tag BAB_RS24320) was associated with Brucella virulence. Determination of bacterial growth curves and resistance to environmental stress factors showed that Pyk plays an important role in B. abortus growth, especially under the conditions of nutrition deprivation, and resistance to oxidative stress. Additionally, cell infection assay showed that Pyk is necessary for B. abortus survival and evading fusion with lysosomes within RAW264.7 cells. Moreover, animal experiments exhibited that the Pyk deletion significantly reduced B. abortus virulence in a mouse infection model. Our results elucidated the role of the Pyk in B. abortus virulence and provided information for further investigation of Brucella virulence associated carbon metabolism.  相似文献   

An investigation into an outbreak of pancytopenia in cats in the United Kingdom     

Barbara Glanemann  Karen Humm  Camilla Pegram  Daniel L. Chan 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(1):117-125

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猪痢疾密螺旋体的冻干保存试验     

苏雄  陈毓川  金梅林 《华中农业大学学报》1988,(4)

将密螺旋体菌株(包括猪痢疾密螺旋体和无害螺旋体)接种于华农1或3号血液琼脂平板上,置CO_2:H_2=20:80的环境中和37℃下培养5天。选择生长最好的琼脂平板,每个加入3ml PBS(0.01mol,pH7.2),洗下培养物,集中后加入等容积的S. H. 保护剂并使成均一的悬液分装安瓿瓶。用Edwards EF03型冻干机进行冷冻真空干燥,在真空状态下密封安瓿瓶,冻干菌种保存于4℃冰箱或-10～-15℃冰盒中。保存期:用新鲜保护剂者为8-13个月;用干燥保护剂者为二年。  相似文献   

Trade‐off between defense priming by herbivore‐induced plant volatiles and constitutive defense in tomato     

Peng‐Jun Zhang  Chan Zhao  Zi‐Hong Ye  Xiao‐Ping Yu 《Pest management science》2020,76(5):1893-1901

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温室大棚钢骨架自动弯折和自动焊接技术及装备          下载免费PDF全文

杜婵  苏安晋  杜娟 《农业工程》2020,10(9):34-37

介绍了钢骨架自动弯折和自动焊接技术及装备的必要性和重要性,以及国内外发展现状和趋势。针对目前骨架制作难、安全性差、现有设备移动不便及消耗大量人力物力等一系列问题,在国内外现有钢骨架制作技术及装备的基础上,自主开发研制一种新型的并适合我国国情的钢骨架自动弯折和自动焊接技术及装备。重点介绍钢骨架自动弯折和自动焊接技术及装备的主要结构组成、工作原理、工作过程和关键技术特点,并对其开发应用前景进行展望。   相似文献   

A typology of agricultural production systems: Capability building trajectories of three Asian economies     

Chan‐Yuan Wong  Guanie Lim 《Asia Pacific viewpoint》2020,61(1):37-53

Analysing the agricultural sectors of Taiwan, Malaysia and Singapore, this paper examines the capability‐building process that encourages productivity and innovation. It describes and explains the origins and subsequent evolution of three forms of agricultural production system, each generating different farming capabilities and distinct forms of competitive advantages. The paper argues that Taiwan's rice‐oriented agricultural production system stimulates both productivity and innovation, helping Taiwanese farmers raise their income level and living standards. The active deployment of state institutions and a malleable labour force, evidenced in the Malaysian palm oil industry, is effective in raising farming productivity but not the ability to innovate. Singapore's aquaculture‐oriented agricultural production system is somewhat useful in stimulating productivity and innovation. Yet the city‐state's inherent lack of space and open international trade regime have circumscribed the potential of its aquaculture industry. It now relies on the regional ethnic Chinese business networks to expand the aquaculture industry's knowledge base and its industrial commons. The principles discussed in this paper provide policy lessons, or at least some initial guidance, for other developing economies aspiring to modernise their agricultural sector.  相似文献