Alterations in activated T‐cell cytokine expression in healthy dogs over the initial 7 days of twice daily dosing with oral cyclosporine     

Caitlin Riggs  Lakshmi Narayanan  Charlee Mulligan  Robert Wills  Andrew Mackin  Claire Fellman  John Thomason  Todd Archer 《Journal of veterinary pharmacology and therapeutics》2019,42(4):385-391

Cyclosporine is a powerful T‐cell inhibitor used in the treatment of immune‐mediated and inflammatory diseases in the dog. There is limited information on how to best monitor patients on cyclosporine therapy. Currently, pharmacokinetic and pharmacodynamic assays are available. Pharmacokinetic assays that measure the concentration of cyclosporine in the blood are used to assess if an appropriate drug concentration has been achieved; however, target blood drug concentrations have not been shown to reliably correlate with suppression of T‐cell function in the dog. In human transplant recipients, therapeutic drug monitoring has shifted to include pharmacodynamic‐based monitoring. Our laboratory has validated a RT‐qPCR assay to measure the pharmacodynamic effects of cyclosporine in the dog. In this study, activated T‐cell expression of IL‐2 and IFN‐γ was measured using RT‐qPCR daily for 7 consecutive days in 8 healthy Walker hounds receiving oral cyclosporine at a dosage of 10 mg/kg every 12 hr. Cytokine production was found to be markedly decreased within 24 hr after the initiation of cyclosporine and remained significantly decreased for the duration of the project. Based on these results, cyclosporine causes a rapid drop in T‐cell cytokine production that is sustained with continued dosing in healthy dogs. Although performed in healthy dogs, this study demonstrated a marked decrease in cytokine suppression within 24 hr of drug administration, suggesting that pharmacodynamic monitoring of cyclosporine's effects on T cells could be considered within several days of commencing therapy in dogs suffering from life‐threatening immune‐mediated disorders.  相似文献   

What's your diagnosis? Skin impression smear from a Bearded Dragon          下载免费PDF全文

Holly M. Minard  Caitlin Burrell  Josue D. Delgado  Raquel R. Rech  Mary B. Nabity 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(3):505-506

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Sensibilité à l'halothane dans quelques élevages de porcs Landrace au Québec          下载免费PDF全文

Lafortune AM  Malo D  Nadeau S  Deroth L 《The Canadian veterinary journal. La revue veterinaire canadienne》1989,30(1):50-51

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Innovative approaches to genome editing in avian species   总被引：1，自引：0，他引：1  

Caitlin A.Cooper  Timothy J.Doran  Arjun Challagulla  Mark L.V.Tizard  Kristie A.Jenkins 《畜牧与生物技术杂志(英文版)》2018,(2)

The tools available for genome engineering have significantly improved over the last 5 years, allowing scientist to make precise edits to the genome. Along with the development of these new genome editing tools has come advancements in technologies used to deliver them. In mammals genome engineering tools are typically delivered into in vitro fertilized single cell embryos which are subsequently cultured and then implanted into a recipient animal.In avian species this is not possible, so other methods have been developed for genome engineering in birds. The most common involves in vitro culturing of primordial germ cells(PGCs), which are cells that migrate through the embryonic circulatory system to the developing gonad and colonize the gonad, eventually differentiating into the gonadocytes which produce either sperm or ova. While in culture the PGCs can be modified to carry novel transgenes or gene edits, the population can be screened and enriched, and then transferred into a recipient embryo. The largest drawback of PGC culture is that culture methods do not transfer well across avian species, thus there are reliable culture methods for only a few species including the chicken. Two newer technologies that appear to be more easily adapted in a wider range of avian species are direct injection and sperm transfection assisted gene editing(STAGE).The direct injection method involves injecting genome engineering tools into the circulatory system of the developing embryo just prior to the developmental time point when the PGCs are migrating to the gonads. The genome engineering tools are complexed with transfection reagents, allowing for in vivo transfection of the PGCs. STAGE utilizes sperm transfection to deliver genome engineering tools directly to the newly fertilized embryo. Preliminary evidence indicates that both methodologies have the potential to be adapted for use in birds species other than the chicken, however further work is needed in this area.  相似文献   

Development of the neonatal B and T cell repertoire in swine: implications for comparative and veterinary immunology     

Butler JE  Sinkora M  Wertz N  Holtmeier W  Lemke CD 《Veterinary research》2006,37(3):417-441

Birth in all higher vertebrates is at the center of the critical window of development in which newborns transition from dependence on innate immunity to dependence on their own adaptive immunity, with passive maternal immunity bridging this transition. Therefore we have studied immunological development through fetal and early neonatal life. In swine, B cells appear earlier in fetal development than T cells. B cell development begins in the yolk sac at the 20th day of gestation (DG20), progresses to fetal liver at DG30 and after DG45 continues in bone marrow. The first wave of developing T cells is gammadelta cells expressing a monomorphic Vdelta rearrangement. Thereafter, alphabeta T cells predominate and at birth, at least 19 TRBV subgroups are expressed, 17 of which appear highly homologous with those in humans. In contrast to the T cell repertoire and unlike humans and mice, the porcine pre-immune VH (IGHV-D-J) repertoire is highly restricted, depending primarily on CDR3 for diversity. The V-KAPPA (IGKV-J) repertoire and apparently also the V-LAMBDA (IGLV-J) repertoire, are also restricted. Diversification of the pre-immune B cell repertoire of swine and the ability to respond to both T-dependent and T-independent antigen depends on colonization of the gut after birth in which colonizing bacteria stimulate with Toll-like receptor ligands, especially bacterial DNA. This may explain the link between repertoire diversification and the anatomical location of primary lymphoid tissue like the ileal Peyers patches. Improper development of adaptive immunity can be caused by infectious agents like the porcine reproductive and respiratory syndrome virus that causes immune dysregulation resulting in immunological injury and autoimmunity.  相似文献   

Chopping of whole-crop barley silage improves intake and live-weight gain of young dairy steers   总被引：1，自引：0，他引：1  

B.-O. Rustas  E. Nadeau 《Livestock Science》2011,141(1):80-84

This experiment evaluated the effect of chopping whole-crop barley silage on intake, diet selection and live-weight gain in young dairy steers. Whole-crop barley harvested at the dough stage of maturity and preserved as round bale silage was fed ad libitum in its long form or precision-chopped to 63 pen-housed dairy steers (average weight 173, s.d. 39 kg). In addition, each animal received 0.6 kg of soybean meal, 0.4 kg of barley grain and 0.08 kg of mineral feed daily. Chopping increased dry matter intake of whole-crop barley silage by 23% and live-weight gain by 22%. Higher concentrations of starch in the orts from steers when fed unchopped silage reflected sorting against grain, probably caused by long awns in the unchopped silage, which were not present in the chopped silage. No sorting against grain occurred in the chopped silage. Chopping increased intake of whole-crop barley silage and live-weight gain of young dairy steers, probably due to negative effects of long awns in the unchopped whole-crop barley silage on intake.  相似文献   

Nonvolcanic tremors deep beneath the San Andreas Fault     

Nadeau RM  Dolenc D 《Science (New York, N.Y.)》2005,307(5708):389

We have discovered nonvolcanic tremor activity (i.e., long-duration seismic signals with no clear P or S waves) within a transform plate boundary zone along the San Andreas Fault near Cholame, California, the inferred epicentral region of the 1857 Fort Tejon earthquake (moment magnitude approximately 7.8). The tremors occur between 20 to 40 kilometers' depth, below the seismogenic zone (the upper approximately 15 kilometers of Earth's crust where earthquakes occur), and their activity rates may correlate with variations in local earthquake activity.  相似文献   

Effects of hydrochloric,valeric, and other volatile fatty acids on pathogenesis of ulcers in the nonglandular portion of the stomach of horses     

Nadeau JA  Andrews FM  Patton CS  Argenzio RA  Mathew AG  Saxton AM 《American journal of veterinary research》2003,64(4):413-417

OBJECTIVE: To identify in vitro effects of hydrochloric acid, valeric acid, and other volatile fatty acids (VFAs) on the pathogenesis of ulcers in the nonglandular portion of the equine stomach. SAMPLE POPULATION: Gastric tissues from 13 adult horses. PROCEDURE: Nonglandular gastric mucosa was studied by use of Ussing chambers. Short-circuit current (Isc) and potential difference were measured and electrical resistance and conductance calculated after tissues were bathed in normal Ringer's solution (NRS) or NRS and hydrochloric, valeric, acetic, propionic, and butyric acids. Treated tissues were examined histologically. RESULTS: Incubation in 60mM valeric acid at pH < or = 7.0 abruptly and irreversibly abolished Isc, which was followed by a slower decrease in resistance and an increase in conductance. Incubation in 60mM acetic, propionic, and butyric acids and, to a lesser extent, hydrochloric acid at pH < or = 7.0 significantly decreased Isc, which was followed by an increase in resistance and a decrease in conductance. CONCLUSIONS AND CLINICAL RELEVANCE: Incubation in valeric acid at pH < or = 7.0 caused a dramatic decrease in mucosal barrier function in the nonglandular portion of the stomach. Changes in barrier function attributable to exposure to valeric acid were associated with histopathologic evidence of cellular swelling in all layers of the nonglandular mucosa. Because of its high lipid solubility, valeric acid penetrates the nonglandular gastric mucosa, resulting in inhibition of sodium transport and cellular swelling. Valeric acid and other VFAs in gastric contents may contribute to the pathogenesis of ulcers in the nonglandular portion of the stomach of horses.  相似文献   

A case of zoonosis associated with Streptococcus equi ssp. zooepidemicus          下载免费PDF全文

Boucher C  Higgins R  Nadeau M  Vincent C 《The Canadian veterinary journal. La revue veterinaire canadienne》2002,43(2):123-124

A sexagenarian couple operating a dairy farm, where there were also many horses, suffered a severe respiratory infection associated with the bacterium Streptococcus equi ssp. zooepidemicus. The investigation revealed that the source of infection was due to contacts with a horse having an infected wound from which we isolated this zoonotic agent.  相似文献   

Parasitologie vétérinaire. Fascicule V: Mycologie vétérinaire          下载免费PDF全文

Marie Nadeau 《The Canadian veterinary journal. La revue veterinaire canadienne》1995,36(9):551

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