Localization of Helicobacter spp. in the fundic mucosa of laboratory Beagle dogs: an ultrastructural study     

Lanzoni A  Faustinelli I  Cristofori P  Luini M  Simpson KW  Scanziani E  Recordati C 《Veterinary research》2011,42(1):42

ABSTRACT: In dogs Helicobacter spp. are found in all gastric regions usually localized in the surface mucus, gastric glands and parietal cells. The aim of this study was to detail the distribution of Helicobacter spp. in the fundic mucosa of asymptomatic Beagle dogs and their intracellular localization within parietal cells, in order to evaluate species-specific pathogenetic effects on gastric cells. The presence of Helicobacter spp. was investigated by immunohistochemistry, TEM, and PCR in the fundic mucosa of six Beagle dogs. Helicobacter spp. were found in all dogs examined, and H. bizzozeronii and H. felis were identified by PCR and confirmed by TEM. In the lumen of the fundic glands, co-localization was common. H. bizzozeronii was present in larger numbers than H. felis in both intraluminal and intraparietal localization. The amounts of H. bizzozeronii were similar in superficial and basal portions of the glands. H. felis was predominantly localized in the superficial portions of gastric glands but almost absent from the base. Within parietal cells, most Helicobacter organisms were intracanalicular, but intact and degenerate Helicobacter organisms were also visualized free in the cytoplasm or in secondary lysosomes. No specific degenerative lesions were found in infected parietal cells. Helicobacter organisms were also observed within macrophages in the lamina propria. In conclusion, there is a differential distribution of H. bizzozeronii and H. felis in the fundic mucosa of Beagle dogs, and their intracellular localization in parietal cells and macrophages suggests novel pathogenic scenarios for the development of immune response and maintenance of chronic gastritis in dogs.  相似文献   

Normal reference intervals and the effects of sample handling on dynamic viscoelastic coagulometry (Sonoclot) in healthy adult horses          下载免费PDF全文

Camilla A. Jamieson BVM & BVS  Andrew S. Hanzlicek DVM  MS  DACVIM  Mark E. Payton PhD  Todd C. Holbrook DVM  DACVIM  DACVSMR 《Journal of Veterinary Emergency and Critical Care》2018,28(1):39-44

Objective

To determine reference intervals and the effect of sample agitation and rest time on Sonoclot analysis in healthy adult horses.

Design

Original prospective study.

Setting

University veterinary medical teaching hospital.

Animals

Sixty healthy adult horses.

Interventions

Blood was collected for assessment of complete blood count, serum biochemical analysis, and Sonoclot analysis.

Measurements and Main Results

Horses were determined to be healthy based upon physical examination, CBC, and serum biochemistry analysis. Blood was analyzed in a glass bead‐containing cuvette using the Sienco Sonoclot analyzer following 2 rest periods (30 mins and 240 min) and with 2 sample handling interventions (agitated and nonagitated), to obtain values for clot rate, time‐to‐peak, activated clotting time, and platelet function. This study failed to detect a significant difference when a rest time of 30 minutes was compared with 240 minutes, but based on wide limits of agreement the 2 rest times were not considered interchangeable. Agitation at both rest times significantly affected all Sonoclot analyses leading to changes indicative of hypercoagulability.

Conclusions

Sample agitation and rest time should be taken into consideration when developing preanalytical guidelines for Sonoclot analysis in horses. Calculated reference intervals were relatively wide. Further research is needed to evaluate the clinical utility of Sonoclot analysis in horses.  相似文献   

Microalgae Schizochytrium sp. as a source of docosahexaenoic acid (DHA): Effects on diet digestibility,oxidation and palatability and on immunity and inflammatory indices in dogs     

Camilla Mariane Menezes Souza  Daniele Cristina de Lima  Taís Silvino Bastos  Simone Gisele de Oliveira  Breno Castello Branco Beiro  Ananda Portella Flix 《Animal Science Journal》2019,90(12):1567-1574

The objective of this study was to evaluate the effects of the microalgae Schizochytrium sp., as a dietary source of docosahexaenoic acid (DHA), on diet palatability, coefficients of total tract apparent digestibility (CTTAD) of nutrients and metabolizable energy (ME), blood variables and indicators of immunity in dogs. We also evaluated oxidative stability. Two diets containing 0 and 0.4% of microalgae Schizochytrium sp. were evaluated in three experiments. On Experiment I the palatability of diets containing 0% versus 0.4% microalgae was compared. In Experiment II test diets were offered for 30 days to determine digestibility, fecal characteristics, and blood parameters. In Experiment III, the oxidative stability of diets containing microalgae versus anchovy oil was evaluated. There was a higher intake ratio of the diet containing microalgae (p < .05). The ME and CTTAD of nutrients increased (p < .05), except for ether extract after acid hydrolysis, with the inclusion of the microalgae in diet. The amount of monocytes and phagocytic granulocytes was higher (p < .05) in dogs fed 0.4% microalgae. There was greater oxidative stability for the sample containing microalgae. The addition of 0.4% microalgae presented high palatability, increased phagocytic cell numbers, and demonstrated oxidative stability superior to anchovy oil.  相似文献   

Prevalence of Neospora caninum and bovine viral diarrhoea virus in dairy cows in Southern Vietnam     

Duong MC  Alenius S  Huong LT  Björkman C 《Veterinary journal (London, England : 1997)》2008,175(3):390-394

The main purpose of this study was to investigate the prevalence of Neospora caninum and bovine viral diarrhoea virus (BVDV) in some dairy herds in Southern Vietnam, and to ascertain whether there were differences in seroprevalences between herds with imported and locally bred cows. Serum samples collected on five state farms and 97 smallholder herds were analysed for the presence of antibodies to N. caninum and BVDV. All BVDV antibody-negative sera were further tested by antigen-ELISA in order to identify persistently infected individuals. The N. caninum prevalence varied between 16% and 53% in the state herds, and was higher in the four herds that had imported cows than in the herd that only had locally bred cows. Nineteen percent of the samples collected on smallholder farms, which all had only locally bred cows, had antibodies to N. caninum. The BVDV seroprevalence varied between 58% and 93% on the state farms. In smallholder herds, the prevalence of BVDV among the sampled cows was 18% and even lower on the state farms. Despite the high seroprevalence for BVDV in the state herds, no persistently BVDV infected cows were found. Given the high prevalence for Neospora and BVDV among herds with imported cows, it seems advisable to test for both infections before cattle are imported into the country.  相似文献   

Buffy coat smear or Knott's test: which to choose for canine microfilaria screening in field studies?          下载免费PDF全文

Ricardo Marcos  Cristina Pereira  Marta Santos  Camilla Luzzago  Stefania Lauzi  João P. Maia  Augusto Faustino  Pablo Puente‐Payo 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2016,45(1):201-205

  相似文献   

Stakeholders' perceptions of consultations as tools for co-management — A case study of the forestry and reindeer herding sectors in northern Sweden     

Camilla Sandstrm  Camilla Widmark 《Forest Policy and Economics》2007,10(1-2):25-35

The forestry and reindeer herding sectors utilize the same land in northern Sweden, and adversely affect each other's productivity. The common pool resource character of this situation has made it difficult to find ways to resolve conflicts that could threaten the two sectors' continued co-existence. A consultation procedure that was introduced to reduce conflicts does not appear to be effective, since conflicts between the two actors still occur. One reason for this failure might be found in the power distribution between forestry and reindeer herding. Earlier research has shown that a co-management system in which the allocation of power between the stakeholders is uneven is difficult to maintain in the long term. However, it is unclear just how uneven the power distribution is between the two actors in this case, and the consequences the disparity might have for the viability and stability of the management system. Focusing on the power relations within the consultation procedures, this paper explores the potential of the present institutional system to take the different interests of the stakeholders into account and to use the consultation procedures as tools for co-managing the forest resources in northern Sweden.  相似文献   

Influence of thermal treatment on black currant (Ribes nigrum L.) juice aroma     

Varming C  Andersen ML  Poll L 《Journal of agricultural and food chemistry》2004,52(25):7628-7636

The influence of thermal treatment on black currant juice aroma was investigated in temperature and time ranges relevant for black currant juice concentration processes, namely, 45, 60, 75, and 90 degrees C. Forty-nine aroma compounds were quantified, and the thermal treatment resulted in concentration increases of most terpenes, aldehydes, furans, and phenols, whereas the concentration of esters slightly decreased. Higher temperatures and longer exposure times had larger effects on the aroma compounds. Odor triangle tests showed no sensory difference between pasteurized juice and juice heated at 60 degrees C, whereas juice heated at 90 degrees C differed significantly from pasteurized juice. It is concluded that a 90 degrees C thermal treatment of black currant juice, which is in the temperature range used for conventional evaporation of black currant juice, has an effect on the aroma and sensory properties.  相似文献   

Consumption and remediation of European Seabass (Dicentrarchus labrax) waste by the sea cucumber Holothuria forskali     

Camilla L. E. MacDonald  Selina M. Stead  Matthew J. Slater 《Aquaculture International》2013,21(6):1279-1290

Integrated multi-trophic aquaculture (IMTA) systems combining finfish and sea cucumbers remain largely untested. Integrating a deposit-feeding species into existing aquaculture units prerequires survival of the deposit feeder, waste diet consumption and bioremediation effect of feeding activity. The sea cucumber, Holothuria forskali, was fed waste from cultured European Seabass, Dicentrarchus labrax, to determine palatability and impact of grazing by H. forskali. Sea cucumbers readily consumed waste diet at amounts suitable to process biodeposition beneath commercial sea-based D. labrax farms. Grazing by H. forskali significantly reduced total nitrogen content of D. labrax waste in short-term controlled feeding experiments (unpaired t test, p < 0.05) and suppressed total carbon content of D. labrax waste in long-term controlled feeding experiments (unpaired t test, p < 0.05). Survival, palatability and nutrient reduction results support investigation of H. forskali inclusion in IMTA systems focussed on bioremediation of finfish waste if limitations in understanding of growth potential, biology and life history are addressed.  相似文献   

Artificial neural networks as a tool for plant identification: a case study on Vietnamese tea accessions   总被引：1，自引：0，他引：1  

Camilla Pandolfi  Sergio Mugnai  Elisa Azzarello  Silvia Bergamasco  Elisa Masi  Stefano Mancuso 《Euphytica》2009,166(3):411-421

Seventeen tea accessions belonging to Chinese (Camellia sinensis), Assamic (C. sinensis var. assamica), and Shan tea (C. sinensis var. pubilimba) groups, which are either commercially planted or new promising tea germplasm, were morphologically described at Phu Tho province (Viet Nam) and assessed for their diversity. Fourteen phyllometric parameters were qualitatively and quantitatively investigated using digital image analysis. The accessions were then discriminated by a dedicated artificial neural network for univocal plant identification and a hierarchical cluster analysis was performed in order to build a dendrogram reporting the relationships among them. Results proved the diversity of investigated tea morphotypes from Phu Tho province based on a morphological screening. More, the artificial neural network was able to perform a correct identification for almost all the accessions using simple dedicated instruments.  相似文献   

Prevalence and transmission of Neospora caninum within infected Swedish dairy herds     

Frössling J  Uggla A  Björkman C 《Veterinary parasitology》2005,128(3-4):209-218

In this long-term study, the changes in within-herd Neospora caninum seroprevalences and the relative importance of horizontal and vertical parasite transmission in Swedish dairy herds were investigated. The within-herd prevalences varied between 5.8% and 65.0% when the herds were first sampled. Comparing the prevalences of year 2000 with those found 2-3 years later, nine out of 14 investigated herds had a similar or lower within-herd prevalence while five herds had an increased portion of seropositive individuals. Three herds were free or almost free from infection at their last sampling. Changes in prevalence were not related to the level of prevalence that each herd had when first sampled. Antibody avidity measurements revealed that the majority of seropositive individuals in all herds were chronically infected. Dam-calf pair investigations and inspection of genealogic trees supplemented with antibody test results showed that vertical transmission was by far the dominant transmission route in all herds. Taken together, these results indicate that the prevalence of N. caninum infection in Swedish dairy cattle can be successfully reduced if actions to eliminate infection in a herd are combined with preventive measures to avoid re-infection.  相似文献