Assessment of antibody avidity in aborting cattle by a somatic Neospora caninum tachyzoite antigen IgG avidity ELISA     

Sager H  Gloor M  Björkman C  Kritzner S  Gottstein B 《Veterinary parasitology》2003,112(1-2):1-10

A Neospora caninum IgG avidity ELISA was carried out on the basis of a somatic N. caninum tachyzoite antigen. The test was validated using experimentally infected calves, where a clear maturation of the IgG avidity over time could be demonstrated. At a maximum of 82 days after infection (d.p.i.), all animals showed antibody avidities ranging above 35%, and respective sera were thus defined as highly avid.Sera of 103 naturally infected seropositive cows with abortion (N. caninum association was provided by a N. caninum PCR-positivity of the fetus in 40 cases) and 139 seropositive animals without abortion history were concurrently examined. Significantly lower avidities were observed in aborting cows when compared to animals without abortion problems (P<0.01). While the avidity of sera collected before abortion remained practically constant until abortion, a significant increase of avidity could be observed in samples collected weeks to months after abortion (P<0.01).The avidities of non-aborting animals from farms with or without abortion problems did not differ significantly with time and were mainly located in the high avidity area. These data indicate that low avidities are not necessarily linked to recent N. caninum infection but can also be an indicator for increased abortion risk in cattle.  相似文献   

Use of bulk milk for detection of Neospora caninum infection in dairy herds in Thailand     

Chanlun A  Näslund K  Aiumlamai S  Björkman C 《Veterinary parasitology》2002,110(1-2):35-44

The relationship between the level of Neospora caninum antibodies in bulk milk and the seroprevalence in lactating cows was investigated. Bulk milk was also used to estimate the prevalence of N. caninum infection in dairy herds in the northeast and north Thailand. Bulk milk and individual serum from all lactating cows in 11 herds as well as 220 bulk milk samples from nine milk collection centres were analysed for presence of N. caninum antibodies using an iscom ELISA. In the 11 herds the bulk milk absorbances ranged between 0.04 and 0.89 and the seroprevalences varied between 0 and 46%. Five herds had milk absorbances below 0.20, among those were the two herds housing only seronegative lactating cows. In the remaining three herds with such low bulk milk absorbances one or two cows (5-14%) were seropositive. Six of the investigated herds had bulk milk absorbances above 0.20. In the two herds with the highest bulk milk absorbances more than 30% of the cows were seropositive. Using an absorbance of 0.20 to discriminate between negative and positive herds, 102 (46%) of 220 bulk milk samples were judged positive. There was no significant difference in mean bulk milk absorbance between the milk collection centres within each region. However, the proportion of herds with bulk milk absorbances > or =0.50 in the north was statistically (P < 0.01) higher than that in the northeast. It was concluded that bulk milk antibody testing can be used to identify N. caninum-infected herds and that N. caninum is a common infection in dairy herds in Thailand.  相似文献   

Cholinesterase in plasma and acetylcholinesterase in erythrocytes from cattle of different breeds and ages and fed different diets     

Kristina Forstend  Camilla Bjrkman 《Acta veterinaria Scandinavica》1983,24(3):328-330

  相似文献   

Effect of time and autologous serum addition on the analysis of cerebrospinal fluid in horses     

Camilla Quattrini  Rebeca Scalco  William Vernau  Pouya Dini  Monica Aleman 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(2):713-717

  相似文献   

Surgical complications following sentinel lymph node biopsy guided by γ-probe and methylene blue in 113 tumour-bearing dogs     

Lavinia Elena Chiti  Elisa Maria Gariboldi  Roberta Ferrari  Ester Luconi  Patrizia Boracchi  Donatella De Zani  Davide Zani  Martina Manfredi  Carlotta Spediacci  Valeria Grieco  Chiara Giudice  Camilla Recordati  Francesco Ferrari  Damiano Stefanello 《Veterinary and comparative oncology》2023,21(1):62-72

Sentinel lymph node biopsy (SLNB) is an accepted veterinary surgical procedure given the impact of early detection of nodal metastases on staging of several canine malignancies. This study aims at reporting the incidence and risk factors for surgical complications of SLNB in tumour-bearing dogs. A total of 113 client-owned dogs that underwent tumour excision and SLNB guided by γ-probing and blue dye were retrospectively enrolled. Recorded variables included: signalment, location and number of extirpated lymphocenters and nodes, time for SLNB, histopathological status of excised nodes. Incidence of SLNB complications was calculated. They were classified as minor and major based on severity and required treatment, and as short-term (0–30 days) and long-term (31–90 days). Univariate analysis with generalized linear model with binomial error estimated the association between variables and incidence of SLNB complications. Significance was set at 5%. Median overall time for SLNB was 25 min. Surgeons excised one node in 38% of dogs and multiple nodes in 62% of cases, belonging to one (62%) or multiple (38%) lymphocenters. Metastases were detected in 45% of nodes. No intraoperative complications occurred. The overall incidence of postoperative complications of SLNB was 21,24%, the majority of which (91.67%) were minor. Only increasing dogs' weight was associated with an increased incidence of SLNB complications (p = .00976). Sentinel lymphadenectomy was associated with a relatively low incidence of complications, most of which were self-limiting. The low morbidity and previously reported impact on staging of SLNB justify its implementation to collect data for prognostic studies.  相似文献   

Thoracic dog bite wounds in dogs: A retrospective study of 123 cases (2003-2016)     

Anna K. Frykfors von Hekkel BVetMed PGDVCP  MRCVS  Camilla Pegram BVetMed  MRes  MRCVS  Zoë J. Halfacree MA VetMB  CertVDI  CertSAS  FHEA  DECVS  MRCVS 《Veterinary surgery : VS》2020,49(4):694-703

  相似文献   

Bone morphogenetic protein 15 supplementation enhances cumulus expansion,nuclear maturation and progesterone production of in vitro-matured bovine cumulus-oocyte complexes     

Juliana de Carvalho Delgado  Thais Rose dos Santos Hamilton  Camilla Mota Mendes  Adriano Felipe Perez Siqueira  Marcelo Demarchi Goissis  José Buratini  Mayra Elena Ortiz D’Ávila Assumpção 《Reproduction in domestic animals》2021,56(5):754-763

In vitro embryo production (IVP) efficiency is reduced when compared to in vivo. The basic knowledge of bovine in vitro oocyte maturation (IVM) mechanisms provides support to improve in vitro embryo production yields. The present study assessed the effects of bone morphogenetic protein 15 (BMP15), fibroblast growth factor 16 (FGF16) and their combined action on cumulus cells (CC) expansion, oocyte and CC DNA fragmentation, oocyte nuclear maturation, energetic metabolism and progesterone production in bovine IVM. Cumulus-oocyte complexes (COC) were matured in control or supplemented media containing BMP15 (100 ng/ml), FGF16 (10 ng/ml) or BMP15 combined with FGF16; and assessed at 0 and 22 hr of IVM. BMP15 alone or its association with FGF16 enhanced cumulus expansion. BMP15 decreased DNA fragmentation in both CC and oocytes, and improved oocyte nuclear maturation rate. In addition, BMP15 increased CC progesterone production, an effect not previously reported. The present study reinforces previous data pointing to a beneficial influence of BMP15 during IVM, while providing novel evidence that the underlying mechanisms involve increased progesterone production.  相似文献   

Soil carbon stock in olive groves agroforestry systems under different management and soil characteristics     

Bateni  Camilla  Ventura  Maurizio  Tonon  Giustino  Pisanelli  Andrea 《Agroforestry Systems》2021,95(5):951-961

The atmospheric concentrations of greenhouse gases have increased to unprecedented levels during last decades, raising concerns about their effect on climate change. Agriculture and land use change play an important role in atmospheric CO₂ emission and fixation, especially by affecting the soil carbon (C) storage. In this context, agroforestry systems (AFSs) could play an important role contributing to climate change mitigation. Given the importance of olive cultivation in the Mediterranean region, it is important to investigate soil C stock in olive groves, and to assess which agronomic practices could improve the soil C stock in these systems. For this reason, a study was conducted in different olive groves, including conventional and organic management, and a typical silvopastoral AFS. Furthermore, an abandoned olive grove and nearby forest were examined as a comparison. Soil samples were collected in each farm and analysed for C content and physico-chemical characteristics. This study indicates that, irrespective of the management, olive groves in the Umbria region of Italy are characterised by a high level of soil C stock if compared to those growing in other areas and to forest ecosystems, indicating that the practices adopted in the area are not negatively affecting soil C storage. A slightly lower soil C stock was measured in the silvopastoral AFS in comparison to the other farms, while high soil C stock was associated with the use of pomace, suggesting that this practice can be used to further increase soil C stock in olive orchards.

  相似文献   

Cell biology. A helix for the final cut     

Raiborg C  Stenmark H 《Science (New York, N.Y.)》2011,331(6024):1533-1534

  相似文献