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31.
Marinaro M Tempesta M Tarsitano E Camero M Losurdo M Buonavoglia C Rezza G 《Research in veterinary science》2012,93(2):662-667
The analysis of cytokines secreted by antigen-specific lymphocytes is hampered in goats by the paucity of species-specific reagents yet it is crucial to study immune responses to infections. To overcome this limit, two commercial kits designed to measure soluble bovine IL-4 (by ELISA) and frequencies of bovine IFN-gamma secreting cells (by ELISPOT) were tested for cross-reactivity in goats. In addition, an ELISA specific to bovine/ovine IL-4 and employing two monoclonal antibodies, clones CC313 and CC314, was tested as well. Concanavalin A-stimulated caprine peripheral blood mononuclear cells (PBMCs) cultures were studied and they exhibited high levels of soluble IL-4 and high frequencies of IFN-gamma secreting cells. In addition, the two IL-4 ELISAs detected similar amounts of cytokine. To start defining the cytokine response triggered by caprine herpesvirus 1 (CpHV-1) infection, PBMC cultures were setup from goats naturally or experimentally infected with CpHV-1. High frequencies of IFN-gamma producing cells and low, when detectable, levels of soluble IL-4 were observed in CpHV-1-specific PBMC cultures from both groups of infected goats. Thus, the availability of cross-reactive research tools can expand cytokine studies in goats and can implement the research on immunity to other caprine infections. 相似文献
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Characterisation of canine parvovirus strains isolated from cats with feline panleukopenia 总被引：1，自引：0，他引：1
Nicola Decaro Domenico Buonavoglia Francesca Amorisco Antonio Parisi Gabriella Elia Alessandra Cavalli Canio Buonavoglia 《Research in veterinary science》2010,89(2):275-278
Unlike the original canine parvovirus type 2 (CPV-2), CPV-2 variants have gained the ability to replicate in vivo in cats but there is limited information on the disease patterns induced by these variants in the feline host. During 2008, two distinct cases of parvoviral infection were diagnosed in our laboratories. A CPV-2a variant was identified in a 3-month-old Persian kitten displaying clinical sign of feline panleukopenia (FPL) (acute gastroenteritis and marked leukopenia) and oral ulcerations, that died eight days after the onset of the disease. Two pups living in the same pet shop as the cat were found to shed a CPV-2a strain genetically identical to the feline virus and were likely the source of infection. Also, non-fatal infection by a CPV-2c strain occurred in a 2.5-month-old European shorthair kitten displaying non-haemorrhagic diarrhoea and normal white blood cell counts. By sequence analysis of the major capsid protein (VP2) gene, the feline CPV-2c strain showed 100% identity to a recent canine type-2c isolate. Both kittens had been administered multivalent vaccines against common feline pathogens including FPL virus. Whether and to which extent the FPL vaccines can protect cats adequately from the antigenic variants of CPV-2 should be assessed. 相似文献
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C. Buonavoglia A. Fioretti M. Tollis F. Menna V. Papparella 《Veterinary research communications》1988,12(2-3):195-197
Inactivation of Newcastle Disease Virus (NDV) by binary ethylenimine (BEI) is reported. The activity of an oil vaccine prepared with BEI-inactivated NDV was compared to a vaccine prepared with formalin-inactivated NDV.The BEI inactivated vaccine had almost twice the efficacy. 相似文献
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C. Buonavoglia M. Tempesta A. Cavalli V. Voigt D. Buonavoglia A. Conserva M. Corrente 《Comparative immunology, microbiology and infectious diseases》1996,19(4):275-281
The authors report CapHV.1 reactivation in two latently infected adult goats treated with dexamethasone (DMS) (4.40 mg/kg BW) for 6 days. Virus was reisolated from vaginal swabs from the 3rd to the 12th day post-treatment with DMS and from nasal swabs for 2 days (6th and 7th day post-treatment). The animals also showed an increase of neutralizing antibody (SN) titer to CapHV.1 3 weeks after the end of treatment with DMS. 相似文献
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Experimental infection of dogs with a novel strain of canine coronavirus causing systemic disease and lymphopenia 总被引：1，自引：0，他引：1
Decaro N Campolo M Lorusso A Desario C Mari V Colaianni ML Elia G Martella V Buonavoglia C 《Veterinary microbiology》2008,128(3-4):253-260
A pantropic canine coronavirus (CCoV) strain (CB/05) has been recently associated to a fatal outbreak of systemic disease in young dogs. We report the clinical, virological and serological findings in dogs experimentally infected with strain CB/05. The dogs, three 2.5-month-old and two 6-month-old pups, were successfully infected, shedding viral RNA with their faeces for the entire observation period (21 days) and displaying systemic clinical signs resembling those observed during the course of natural infection. Leucopenia (acute lymphopenia) occurred in all infected dogs, with values dropping below 60% of the initial counts. Considering the severity of the CB/05-induced disease, two of the youngest pups were euthanized for ethical reasons at days 8-9 postinfection, whereas the other pups underwent a slow but progressive improvement of their clinical status with complete recovery. At postmortem examination, remarkable lesions were observed in the internal organs of the euthanized pups, that tested positive for CCoV by real-time RT-PCR and virus isolation on cell cultures. All pups seroconverted for CCoV, as shown by the high optical density values and antibody titres detected by ELISA and virusneutralisation tests, respectively. The present study confirms that strain CB/05 is highly pathogenic for dogs, being able to induce a severe disease (and in some cases the death) even in experimental conditions. 相似文献
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Pratelli A Martella V Elia G Tempesta M Guarda F Capucchio MT Carmichael LE Buonavoglia C 《Journal of veterinary medicine. B, Infectious diseases and veterinary public health》2001,48(5):385-392
An outbreak of dual infection in dogs with canine adenovirus type 1 (CAV-1) and canine coronavirus (CCV) infection is reported in an animal shelter that comprised approximately 200 adults stray dogs and 30 puppies. Twenty puppies died 7-8 days after the onset of the clinical signs (severe enteritis, leucopoenia, respiratory distress and dehydration). Both CAV-1 and CCV were isolated from tissue or swab samples. Antibodies to CCV and, at high levels, to CAV-1 also were detected in several puppies. The principal histological findings were atrophy of small intestinal villi, lymphoid depletion, hepatitis and bronchopneumonia. The persistence of CCV in the faeces, observed by the polymerase chain reaction assay, was longer than previously reported. Results demonstrated the serious consequences which may occur with dual infections by CAV-1 and CCV in assembled groups of dogs that are housed in poorly managed kennels with inadequate vaccination programmes. 相似文献