Electro-acupuncture and Chinese herbs for treatment of cervical intervertebral disk disease in a dog     

Ayne Murata Hayashi  Júlia Maria Matera  Tatiana Soares da Silva  Ana Carolina Brand?o de Campos Fonseca Pinto  Sílvia Renata Gaido Cortopassi 《Journal of veterinary science (Suw?n-si, Korea)》2007,8(1):95-98

A non-ambulatory dog with tetraparesis following a pain episode that had evolved over 2 months was submitted for medical treatment and diagnosed with intervertebral disk disease at C3-C4 and dorsal extradural compression at C1-C2 and C3-C4 using myelography and computed tomography. The dog experienced ambulation recovery after 15 days of treatment with only electroacupuncture and Chinese herbal medicine, with marked improvement occurring after only 10 treatments. Six months of follow-up demonstrated that the dog was stable and had no recurrence of symptoms. Therefore, it was concluded that the combination of electroacupuncture and Chinese herbal medicine was responsible for motor rehabilitation.  相似文献   

Retrospective study of 942 small-sized dogs: Prevalence of left apical systolic heart murmur and left-sided heart failure, critical effects of breed and sex     

Pierre Serfass DVM  Valrie Chetboul DVM  PhD  Dipl ECVIM  Carolina Carlos Sampedrano DVM  MS  Audrey Nicolle DVM  Thierry Benalloul DVM  Herv Laforge DVM  Christophe Gau DVM  Carole Hbert DVM  Jean-Louis Pouchelon DVM  PhD  Renaud Tissier DVM  PhD 《Journal of Veterinary Cardiology》2006,8(1):11-18

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Monoclonal antibodies putatively identifying porcine B cells     

S. Denham  R. J. Zwart  J. T. D. Whittall  M. Pampusch  A. H. Corteyn  A. T. J. Bianchi  M. P. Murtaugh  R. M. E. Parkhouse  H. Tlaskalova  J. Sinkora  M. Sinkora  Z. Rehakova 《Veterinary immunology and immunopathology》1998,60(3-4):317-328

Comparison was made of the binding of 38 test and three standard monoclonal antibodies (mAbs) to B cells from various pig lymphoid tissues by flow cytometry (FCM) and immunohistochemistry. Some mAbs were also tested on B cells from foetal pig tissues. Twenty of the new mAbs bound, though to variable degrees, to porcine B cells but only three were given cluster assignations: C35 (#147) and BB6-11C9 (#167) were assigned to wCD21 and 2F6/8 (#057) was assigned to SWC7.  相似文献   

Detection of Leishmania spp. and associated inflammation in ocular‐associated smooth and striated muscles in dogs with patent leishmaniosis     

Carolina Naranjo  Dolors Fondevila  Marta Leiva  Xavier Roura  Teresa Pea 《Veterinary ophthalmology》2010,13(3):139-143

Objective Canine leishmaniosis is a disease characterized by the wide distribution of the parasite throughout the tissues of the host. The purpose of this study was to describe the presence of Leishmania spp. and associated inflammation in ocular‐associated muscles of dogs with patent leishmaniosis. Procedures Smooth muscles (iris dilator muscle, iris sphincter muscle, ciliary muscle, Müller muscle, smooth muscle of the periorbita and smooth muscle of the nictitating membrane) and striated muscles (orbicularis oculi muscle, obliquus dorsalis muscle and dorsal rectus muscle) were evaluated. Routine staining with hematoxylin and eosin and immunohistochemistry to detect Leishmania spp. were performed on tissue sections. Results Granulomatous inflammation was seen surrounding muscular fibers and was composed mainly of macrophages with scattered lymphocytes and plasma cells. This infiltrate could be seen in 52/473 (10.99%) samples of smooth muscle and 36/142 (25.35%) samples of striated muscle. Parasites were detected in 43/473 (9.09%) samples of smooth muscle and in 28/142 (19.71%) samples of striated muscle. Conclusions To the authors’ knowledge, this is the first report assessing the presence of Leishmania spp. and associated infiltrate in intraocular, extraocular and adnexal smooth and striated muscles. The inflammation present in those muscles could contribute to clinical signs already described, such as blepharitis, uveitis, and orbital cellulitis.  相似文献   

Growth,Feed Utilization,and Glycemic Response in European Sea Bass,Dicentrarchus labrax,Juveniles Fed Carbohydrate of Different Complexities     

Paula Enes  Helena Peres  Ivone Almeida  Ana Couto  Aires Oliva‐Teles 《Journal of the World Aquaculture Society》2011,42(6):873-879

The effect of dietary carbohydrate complexity on growth, feed utilization, and glycemia was studied in European sea bass juveniles. Four isonitrogenous (50% crude protein) and isolipidic (15% crude lipids) diets were formulated to contain 20% pregelatinized maize starch (PGS diet), dextrin (DEX diet), maltose (MAL diet), or glucose (GLU diet). No effect of dietary carbohydrate complexity on growth was noticed. Feed efficiency and protein efficiency ratio were lower in fish fed the GLU diet than in the other groups, whereas the opposite was observed for feed intake. Plasma glucose peaked 3–4 h after feeding in fish fed the MAL and GLU diets, whereas in fish fed the PGS and DEX diets the peak was reached 5–6 h after feeding. Peak plasma glucose concentration (13 mmol/L) was higher in fish fed the GLU diet than the other diets (9 mmol/L). Shorter hyperglycemia duration was observed in fish fed the MALT and GLU diets (6 h) than the PGS and DEX diets (10 h). Complex carbohydrates delayed plasma glucose concentration peak compared with simple sugars, whereas the opposite was observed for hyperglycemia duration. Overall, dietary maltose, dextrin, and starch were apparently better utilized as energy source than glucose by European sea bass juveniles.  相似文献   

The use of monoclonal antibodies in an enzyme immunospot assay to detect isotype-specific antibody-secreting cells in pigs and chickens   总被引：1，自引：0，他引：1  

A T Bianchi  J W Scholten  I M Jongenelen  G Koch 《Veterinary immunology and immunopathology》1990,24(2):125-134

Monoclonal antibodies directed against porcine immunoglobulin isotypes G, G1, G2, M, and A and against chicken immunoglobulin isotopes G, M, and A were tested in an antigen-specific spot-forming cell (SFC) assay based on the principle of the enzyme immunoassay. The SFC assay was used to quantitate ovalbumin (OA)-specific antibody-secreting cells (ASC) in pigs that had been primed and boosted with OA. The SFC assay was also used to quantitate trinitrophenyl (TNP)-specific ASC in chickens that had been primed with TNP-conjugated keyhole lympet haemocyanin (TNP-KLH). Although, the classical plaque-forming cell (PFC) assay cannot reliably detect isotope-specific ASC in pigs and chickens, it can detect these cells in mice. Therefore, we compared the OA- and TNP-specific SFC assays with PFC assays that were specific for these antigens in mice. The study demonstrated that the SFC assay is superior to the PFC assay in detecting both OA-specific ASC and TNP-specific ASC. The frequencies of OA-specific and TNP-specific SFC detected in mice were of the same order of magnitude as those detected in pigs and chickens. We concluded that the SFC assay is the better method for quantitating ASC in pigs, chickens, and probably all domestic animals for which isotype-specific monoclonal antibodies are available.  相似文献   

Tracking leaf area index and coefficient of light extinction over the harvesting cycle of black wattle     

Alexandre Behling  Carlos Roberto Sanquetta  Ana Paula Dalla Corte  Sylvio Péllico Netto  Aurélio Lourenço Rodrigues  Bráulio Otomar Caron  Augusto Arlindo Simon 《林业研究》2016,27(6):1211-1217

The amount of photosynthetic radiation intercepted by a crop is a function of the incident solar radiation on the plants, the leaf area index (L _AI), and the light extinction coefficient (k). We quantified L _AI and k in stands of black wattle (Acacia mearnsii De Wild.) over a 7-year growth cycle at two locations in the state of Rio Grande do Sul, Brazil. Our study was conducted in commercial stands in agroecological regions with high densities of black wattle plantations. L _AI was calculated as the ratio between the leaf area of a tree and its planting space, and k was derived from Beer’s law. L _AI depends on the planting site and stand age. Between the two sites, the L _AI was similar over time, the amount of variation differed. Values of k depended only on stand age, with the highest average observed for stands up to 5 years old. The trend of k during the plantation cycle was inversely proportional to L _AI and was correlated with L _AI, leaf area, leaf dry mass, canopy volume, height, branches dry mass, total dry mass, and crown diameter.  相似文献   

Risk factors to incidental leptospirosis and its role on the reproduction of ewes and goats of Espírito Santo state,Brazil     

Cortizo  Priscilla  Loureiro  Ana Paula  Martins  Gabriel  do Rodrigues  Patrícia Rosário  Faria  Braulio Pego  Lilenbaum  Walter  Deminicis  Bruno Borges 《Tropical animal health and production》2015,47(1):231-235

Ovine and caprine stockbreeding have been gaining attention in developing countries as an attractive investment. On these animals, infectious diseases of the reproductive tract, such as leptospirosis, can compromise the production leading to economic losses. The present study aimed to determine the risk factors associated with incidental leptospirosis and its influence on the reproductive parameters of ewes and goats of Espírito Santo state, Brazil. A total of 737 animals distributed on 24 herds/flocks were studied, and an overall prevalence of 10.9 % seroreactive animals was observed. Serogroup Icterohaemorrhagiae was the most frequent in goats (97.0 %) as well as in ewes (78.3 %). Regarding risk factors related to leptospirosis, the presence of waterholes and the semi-intensive breeding system were the most important associated to seroreactivity. Besides, there was an observed association between seroreactivity and reproductive failures (P?<?0.05). Moreover, seroreactive ewes (relative risk (RR)?=?1.3) and goats (RR?=?1.9) presented more chances to have abortions than seronegative animals. Furthermore, seroreactive ewes presented 11.6 more chances of having premature births when compared to the seronegative ones. It can be concluded that Leptospira infection, mainly those caused incidental strains (such as Icterohaemorrhagiae serogroup), is a significant factor to reduce the productivity of small ruminants’ herds/flocks in the studied region, and environmental measures must be considered on control programs.

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Subcutaneous administration of paclitaxel in dogs with cancer: A preliminary study     

Daniella M. Silva  Aline I. Franciosi  Paula C.F. Pezzini  Simone D. Guérios 《The Canadian veterinary journal. La revue veterinaire canadienne》2015,56(8):823-830

Intravenous paclitaxel has been underused in dogs due to severe and acute hypersensitivity reactions. Subcutaneous (SC) administration of paclitaxel and its safety are unknown. In this preliminary study, SC administration of paclitaxel was evaluated for hypersensitivity reactions and toxicity in 21 dogs with advanced cancer. Dogs received 1 to 5 paclitaxel doses, ranging from 85 to 170 mg/m², SC every 14 or 21 days. A total of 40 paclitaxel doses were administered and none of the 21 dogs developed systemic or acute local hypersensitivity reactions. Severe skin lesions at the injection site developed in 2 dogs after the 4th injection at the same location. Grade 4 neutropenia was observed in 50% of the dogs 5 days after the first treatment at 115 mg/m² (n = 14). Two animals developed Grade 5 diarrhea and died likely due to hemodynamic failure or sepsis. Paclitaxel can be administered SC in dogs with no hypersensitivity reaction.  相似文献   

Experimental Infections of Bluegill with the Trematode Ribeiroia ondatrae (Digenea: Cathaemasiidae): Histopathology and Hematological Response     

Dana M. Calhoun  Paula A. Schaffer  Jacklyn R. Gregory  Katherine M. Hardy  Pieter T. J. Johnson 《Journal of aquatic animal health》2015,27(4):185-191

Infections by the digenetic trematode, Ribeiroia ondatrae, cause severe limb malformations in many North American amphibians. Ribeiroia ondatrae also infects fishes as second intermediate hosts, but less is known about the pathology and immune responses initiated in infected fish, even though reports of infected fish date back to early 1900s. To this end, we experimentally exposed juvenile Bluegills Lepomis macrochirus to three doses of R. ondatrae cercariae and monitored the pathology, parasite infection success, and humoral responses over 648 h. All exposed fish became infected with metacercariae, and the average infection load increased with exposure dose. Histologically, infection was associated with acute hemorrhages in the lateral line and local dermis at 36 h, followed by progressive granulomatous inflammation that led to the destruction of encysted metacercariae. Correspondingly, over the course of 648 h we observed an 85% decline in average infection load among hosts, reflecting the host's clearance of the parasite. Infection was not associated with changes in fish growth or survival, but did correlate with leukocytosis and neutrophilia in circulating host blood. Understanding the physiological responses of R. ondatrae in Bluegill will help to clarify the ecological effects of this parasite and provide a foundation for subsequent comparisons into its effects on behavior, individual health, and population dynamics of Bluegill.

Received March 4, 2015; accepted August 9, 2015  相似文献