Pregnancy Rate in Zebu Cows with Two Different Postpartum Intervals Exposed to a Two-Bull Rotational System     

Molina R  Galina CS  Maquivar M  Estrada S  Chávez A  Díaz GS 《Veterinary research communications》2003,27(8):671-680

In order to evaluate the reproductive effect of a rotational breeding system, the pregnancy rate of 69 Zebu cows was assessed, 32 late postpartum (211±93 days postpartum, LP) and 37 early postpartum (averaging 63±8 days after calving, EP). In the LP group, 60% (19/32) were cycling before exposure to the bulls but only 38% (14/37) in the EP group. The two groups were dissimilar from the start (p<0.05). The cows were exposed in a rotational breeding system, with six Brahman bulls with previous sexual experience paired off in three groups (A–B, C–D and E–F). The cows were exposed to each pair of bulls for a period of 3 weeks, with one week in between each period, when the females were without the presence of a male. In the LP group, the pair of bulls A–B obtained a pregnancy rate of 72% (23/32), whereas for bulls C–D the pregnancy rate was 33% (3/9) and for bulls E–F the pregnancy rate was 67% (4/6). In contrast, for the EP cows, bulls A–B obtained a pregnancy rate of 32% (12/37), bulls C–D a pregnancy rate of 67% (12/18) and for bulls E–F a pregnancy rate of 55% (6/11). The percentage pregnancy obtained using the pair of bulls A–B differed (p<0.05) between LP and EP, whereas for bulls C–D and E–F it was similar. The pregnancy rate was different (p<0.05) in the first 3 weeks of mating for the LP group, the highest number of cows becoming pregnant during the first 3 weeks of the study. In contrast, the highest number of pregnancies in EP occurred evenly during the last 6 weeks. This study suggests that the reproductive performance of pairs of bulls in a 9-week rotational programme with the overall pregnancy rate is similar (94% in the LP and 81% in the EP). However, the time taken for the females to become pregnant, and hence the performance of the bulls, is related to the average number of days postpartum for the cows.  相似文献   

Equine infectious anemia in mules: virus isolation and pathogenicity studies     

Spyrou V  Papanastassopoulou M  Psychas V  Billinis Ch  Koumbati M  Vlemmas J  Koptopoulos G 《Veterinary microbiology》2003,95(1-2):49-59

There appears to be a lack of information concerning responses of mules to natural infection or experimental inoculation with equine infectious anemia virus (EIAV). In the present study EIAV was isolated from mules, for the first time, and its pathogenicity in naturally infected and experimentally inoculated animals was investigated. Two naturally infected (A and B) and three EIAV free mules (C, D and E) were used for this purpose. Mule A developed clinical signs, whereas mule B remained asymptomatic until the end of the study. Mules C and D were each inoculated with 10ml of blood from mule A and developed signs of the disease; they were euthanatized or died at day 22 and 25 post-inoculation, respectively. Mule E served as a negative control. The virus was isolated from the plasma samples of mules with clinical signs of the disease (A, C and D), but not from the asymptomatic mule B. Both proviral DNA and viral RNA were amplified from blood and tissues of the infected animals by nested polymerase chain reaction (nPCR). Antibodies were not detected in the two experimentally infected mules until their natural death or euthanasia. Clinicopathological and laboratory findings showed that, in mules, EIAV produced clinical signs similar to those observed in horses and ponies. Nested PCR proved to be a rapid, sensitive and specific diagnostic method for the detection of EIAV, regardless of the disease stage.  相似文献   

Definition of the flight period of Lasioderma serricorne (F.) in stored tobacco     

S.Ch. Papadopoulou  C.Th. Buchelos 《Anzeiger für Sch?dlingskunde》2002,75(3):81-83

A 4-year (1996–99) survey of the biology of L. serricorne (F) (Coleoptera, Anobiidae) on stored tobacco has taken place in Thessaloniki, northern Greece. For the trapping of the adults, pheromone ('New Serrico' and 'Black Stripe Moth') as well as electric (black light) traps were used. The first generation adults emerging from larvae hibernating inside the tobacco bales appeared flying on the 17/5, 16/5, 28/4, and 30/4 of the years 1996, 1997, 1998, and 1999, respectively. In parallel observations, adults inside the bales were found on the 14/5, 12/5, 23/4, and 26/4 of the above-mentioned years, respectively. Termination of flights, in all the years concerned, took place during October or November, while the last flights of November were recorded in temperatures of about 13°C.  相似文献   

Major Gene and Polygenic Resistance to Leptosphaeria maculans in Oilseed Rape (Brassica napus)     

R. Delourme  A. M. Chèvre  H. Brun  T. Rouxel  M. H. Balesdent  J. S. Dias  P. Salisbury  M. Renard  S. R. Rimmer 《European journal of plant pathology / European Foundation for Plant Pathology》2006,114(1):41-52

The most common and effective way to control phoma stem canker (blackleg) caused by Leptosphaeria maculans in oilseed rape (Brassica napus) is through the breeding of resistant cultivars. Race specific major genes that mediate resistance from the seedling stage have been identified in B. napus or have been introgressed from related species. Many race specific major genes have been described and some of them are probably identical in B. napus (allotetraploid AACC) and the parental species B. rapa (diploid AA). More work is needed using a set of well-characterised isolates to determine the number of different major resistance genes available. In some B. napus cultivars, there is resistance which is polygenic (mediated by Quantitative Trait Loci) and postulated to be race non-specific. Many of these major genes and Quantitative Trait Loci for resistance to L. maculans have been located on B. napus genetic maps. Genes involved in race specific and polygenic resistance are generally distinct.  相似文献   

A Severe Hellenic CMV Tomato Isolate: Symptom Variability in Tobacco, Characterization and Discrimination of Variants     

A. P. Sclavounos  A. E. Voloudakis  Ch. Arabatzis  P. E. Kyriakopoulou 《European journal of plant pathology / European Foundation for Plant Pathology》2006,115(2):163-172

A severe strain of Cucumber mosaic virus (CMV) originating from an infected tomato plant (Gastouni-Olympia, Greece) was isolated in tobacco (Nicotiana tabacum cv. Xanthi nc), after three serial local lesion passages in Chenopodium quinoa and designated CMV-G. CMV-G induces yellow mosaic (YM) symptoms in tobacco. When CMV-G was passed mechanically through C. quinoa, phenotypic variants inducing YM or green mild mosaic (MM) in tobacco were isolated. Aphid transmission, from different hosts, appears to be an effective approach for separating MM variants of CMV-G from YM variants. In particular, aphid transmission from zucchini proved to be very efficient in selecting for MM variants. In contrast, aphids transmitted only YM variants from tomato plants. Molecular characterization of CMV-G and its progeny resulted in their classification in the CMV subgroup IB, free of satellite RNA, being the first discovery of the subgroup IB in Greece. In the Solanaceae family (tobacco, tomato, pepper) YM variants induced more severe symptoms than the MM variants. YM and MM phenotype was stable in tobacco for all seven passages tried using the obtained YM and MM variants. Cross-protection experiments showed that an isolated MM variant was able to protect tobacco plants against a challenge infection by a YM variant.  相似文献   

Inoculation of cucumber roots with zoospores of mycoparasitic and plant pathogenic Pythium species: Differential zoospore accumulation, colonization ability and plant growth response     

E.G. Wulff  A.T.H. Pham  M. Chérif  P. Rey  Y. Tirilly  J. Hockenhull 《European journal of plant pathology / European Foundation for Plant Pathology》1998,104(1):69-76

Hydroponically grown cucumber (Cucumis sativus) seedlings were inoculated with zoospores of 1 mycoparasitic (Pythium oligandrum) and 2 pathogenic (Pythium aphanidermatum and Pythium group F) Pythium spp. During the first 2 days after inoculation, all the Pythium spp. caused reduction in the root length. However, roots treated with Pythium oligandrum quickly reached the length of the control and on the 8th day, and for the rest of the experimental period, stimulation of root elongation was noted. Pythium oligandrum was not pathogenic on cucumber and no differences in the fresh weights of control and Pythium oligandrum inoculated plants were observed in the course of the experiment. Pythium group F and Pythium aphanidermatum were pathogenic on cucumber seedlings, but their pathogenicities differed. Thus, while Pythium group F had a constant, negative influence on root length and plant growth, measured as fresh weight, Pythium aphanidermatum caused generalized necroses of the root system, inhibiting consistently root elongation and plant growth and finally causing plant death. Moreover, the zoospores of 2 mycoparasitic species, Pythium oligandrum and Pythium periplocum, were not attracted to roots of cucumber and accumulated on the roots in very low numbers compared to those of the pathogenic species, Pythium aphanidermatum, which were strongly attracted and accumulated in large numbers. Finally, it was also found that Pythium oligandrum colonized the roots very poorly, while Pythium group F and Pythium aphanidermatum were significantly better root colonizers. The significance of these findings is discussed in relation to the ecology of Pythium species and biocontrol.  相似文献   

Ultrasonographic comparison of adhesions induced by two different methods of gastropexy in the dog     

F. Tanno    U. Weber    Ch.  Wacker  L. Gaschen  V. Schmid  J. Lang 《The Journal of small animal practice》1998,39(9):432-436

Ultrasonography was used to compare adhesions induced by two different methods of gastropexy in 16 dogs. An incisional gastropexy technique was used in eight dogs (group 1) and a 'modified' gastropexy technique in the remaining eight (group 2). The length and thickness of the gastropexy and the peristaltic activity of the stomach were measured ultrasonographically and compared between groups. Measurements for the two groups were taken in the early postoperative interval (two to four days), intermediate postoperative interval (eight to 20 days) and late postoperative interval (57 to 79 days). Both techniques were equally successful in forming permanent adhesions at two months postoperatively and there was no recurrence of gastric dilatation and volvulus. The length and thickness of the gastropexy were similar for both groups at two months postoperatively and there were no surgical complications with either technique. The modified gastropexy provides a technique that can easily be performed by a single surgeon , with no increased operative time or duration of anaesthesia, and with the formation of a permanent adhesion.  相似文献   

Editorial | Comparative Immunology,Microbiology and Infectious Diseases - Volume 31, Issues 2–3     

Ch. Pilet 《Comparative immunology, microbiology and infectious diseases》2008,31(2-3):71

  相似文献   

The effect of Trifluralin on the formation of ectotrophic mycorrhizae in some pine species I. Toxicity to mycorrhiza forming fungi     

Ch. Iloba 《Forest Pathology》1977,7(1):47-51

Laboratory experiments were conducted to test the effect of various concentrations of Trifluralin on certain fungi. The ectotrophic mycorrhizal fungi tested were: Amanita citrina Schaefer, Tricholoma pessundatum (Fr.) Quel., and Tricholoma saponaceum (Fr.) Qtiel. All exhibited sensitivity to Trifluralin concentrations. The consequence of such fungitoxicity was discussed.  相似文献   

The effect of Trifluralin on the formation of ectotrophic mycorrhizae in some pine species     

Ch. Iloba 《Forest Pathology》1977,7(3):172-177

Pot experiments were performed to investigate the effects of Tritluralin on the mycorrhizal symbiosis of conifer seedlings. Infected forcst soils and potting mixturcs were mixed, pottcd and treated with Trifluralin at levels of 0.2 and 0.4ml/m² Seeds of Pinus sylvestris L. and Picea abies (L.) Karst. were sown in the pots. The concentrations tested, though recommended by the manufacturer produced phytotoxicity. The seedling growth in general and that of the rootsystem and laceral roots in particular, were severely inhibited. Mycorrhizal development in the seedlings was consequently adversely affected.  相似文献