Lucidenic acid B induces apoptosis in human leukemia cells via a mitochondria-mediated pathway   总被引：2，自引：0，他引：2  

Hsu CL  Yu YS  Yen GC 《Journal of agricultural and food chemistry》2008,56(11):3973-3980

Ganoderma lucidum is known as a medicinal mushroom used in traditional Chinese medicine. In the present study, the effect of lucidenic acids (A, B, C, and N) isolated from a new G. lucidum (YK-02) on induction of cell apoptosis and the apoptotic pathway in HL-60 cells were investigated. The results demonstrated that lucidenic acids decreased cell population growth of HL-60 cells, assessed with the MTT assay. The cell cycle assay indicated that treatment of HL-60 cells with lucidenic acid A, C, and N caused cell cycle arrest in the G 1 phase. Lucidenic acid B (LAB) did not affect the cell cycle profile; however, it increased the number of early and late apoptotic cells but not necrotic cells. Treatment of HL-60 cells with LAB caused loss of mitochondria membrane potential. Moreover, the ratio of expression levels of pro- and antiapoptotic Bcl-2 family members was changed by LAB treatment. LAB-induced apoptosis involved release of mitochondria cytochrome c and subsequently induced the activation of caspase-9 and caspase-3, which were followed by cleavage of poly(ADP-ribose) polymerase (PARP). Pretreatment with a general caspase-9 inhibitor (Z-LEHD-FMK) and caspase-3 inhibitor (Z-DEVD-FMK) prevented LAB from inhibiting cell viability in HL-60 cells. Our finding may be critical to the chemopreventive potential of lucidenic acid B.  相似文献   

Treatment with taurine attenuates hepatic apoptosis in NZB/W F1 mice fed with a high-cholesterol diet     

Hsu TC  Chiang SY  Wu JH  Tsai CC  Huang CY  Chen YC  Tzang BS 《Journal of agricultural and food chemistry》2008,56(20):9685-9691

Cholesterol-rich diets are known to cause hepatic apoptosis, which has been associated with the pathogenesis of systemic lupus erythematosus (SLE). However, the mechanisms and treatments for hepatic apoptosis in SLE are poorly understood. To clarify the effects of taurine on hepatic apoptosis in SLE, NZB/W F1 mice received control, cholesterol, and cholesterol/taurine diets. Significant reductions of caspase-3 activity, TUNEL-positive cells, and Fas- and mitochondrial- dependent apoptosis were detected in liver from the cholesterol/taurine group as compared to the cholesterol group. Moreover, significant increases of phosphorylated AKT, NF-kappaB (p65), and ERK1/2 proteins were detected in liver from the cholesterol/taurine group as compared to the cholesterol group. In contrast, a significant reduction of phosphorylated p38 protein was observed in the cholesterol/taurine group. These experimental results demonstrated positive effects of taurine against hepatic apoptosis in NZB/W F1 mice fed a high-cholesterol diet and suggested the therapeutic potential of taurine in SLE.  相似文献   

Modeling risk analysis for rice production due to agro-climate change and uncertainty in irrigation water     

Wu  Shiang-Jen  Chiueh  Ya-Wen  Hsu  Chih-Tsung 《Paddy and Water Environment》2018,16(1):35-53

Paddy and Water Environment - This study proposes a risk analysis model for quantifying the insufficient risk of rice production due to the climate change and variation in irrigation water and...  相似文献   

Evaluation of Commercial‐scale Approaches for Cryopreservation of White Crappie,Pomoxis annularis,Sperm          下载免费PDF全文

Charlie M. Culpepper III  Amy M. Guitreau  Shay Allred  Terrence R. Tiersch  Peter J. Allen 《Journal of the World Aquaculture Society》2018,49(4):725-734

Crappie, Pomoxis spp., are popular game fish throughout North America and are produced by public and private hatcheries. However, production is limited by a lack of information on tank culture and induced spawning methods. Development of techniques for storage of sperm and in vitro fertilization would increase flexibility in spawning. Therefore, techniques for sperm cryopreservation were examined in white crappie, Pomoxis annularis. Sperm from adult wild white crappie were used to evaluate sperm extender, cryoprotectant agent and concentration, and cooling technique based on post‐thaw sperm motility. Percent egg fertilization was also compared between sperm stored in the two best cryopreservation protocols and two different osmotic activator solutions. Sperm were cryopreserved using treatment combinations of two extenders (350 mOsmol/kg Hanks' balanced salt solution [HBSS] and 350 mOsmol/kg Ca²⁺free HBSS) and two cryoprotectants (dimethyl sulfoxide [DMSO] and methanol) at concentrations of 5, 10, and 15% that were cooled at four different rates: 5, 10, 20, and 40 C/min. Post‐thaw sperm motility and fertilization rates indicated white crappie sperm can be cryopreserved using either extender, cryoprotectants of either 5% DMSO or 10% methanol, and cooling at 40 C/min. A follow‐up experiment demonstrated sperm in suspensions on ice retained viability after overnight transport.  相似文献   

Happy Birthday Geoff Sumner-Smith from all your friends and colleagues around the world!: Laudation.     

Kenneth Johnson  Josep de la Fuente  John Houlton  Charlie DeCamp  Jean-Pierre Cabassu  Aldo Vezzoni  Dieter Prieur  Amanda Godsell  Andrea Schürg  Dieter Bergemann 《Veterinary and comparative orthopaedics and traumatology》2008,21(3):i-iv

  相似文献   

Nonsurgical repair of an umbilical hernia in two Asian elephant calves (Elephas maximus)     

Ellen B Wiedner  Charlie Gray  Peter Rich  Gary L Jacobson  Ramiro Isaza  Dennis Schmitt  William A Lindsay 《Journal of zoo and wildlife medicine》2008,39(2):248-251

Umbilical hernias were diagnosed in two captive-born, female Asian elephant (Elephas maximus) calves several weeks after birth. Daily manual reduction of the hernias for 5 wk in the first case and for 5 mo in the second resulted in complete closure of the defects. Nonsurgical repair of uncomplicated, fully reducible umbilical hernias in Asian elephants can be an alternative to surgery.  相似文献   

Genetic parameters for semen production traits in Swiss dairy bulls     

Alexander Burren  Hannes Joerg  Malena Erbe  Arthur R. Gilmour  Ulrich Witschi  Fritz Schmitz‐Hsu 《Reproduction in domestic animals》2019,54(9):1177-1181

Variance components (VC) were estimated for the semen production trait ejaculate volume, sperm concentration and sperm motility in the Swiss cattle breeds Brown Swiss (BS), Original Braunvieh (OB), Holstein (HO), Red‐Factor‐Carrier (RF), Red Holstein (RH), Swiss Fleckvieh (SF) and Simmental (SI). For this purpose, semen production traits from 2,617 bulls with 124,492 records were used. The data were collected in the years 2000–2012. The model for genetic parameter estimation across all breeds included the fixed effects age of bull at collection, year of collection, month of collection, number of collection per bull and day, interval between consecutive collections, semen collector, bull breed as well as a random additive genetic component and a permanent environmental effect. The same model without a fixed breed effect was used to estimate VC and repeatabilities separately for each of the breeds BS, HO, RH, SF and SI. Estimated heritabilities across all breeds were 0.42, 0.25 and 0.09 for ejaculate volume, sperm concentration and sperm motility, respectively. Different heritabilities were estimated for ejaculate volume (0.42; 0.45; 0.49; 0.40; 0.10), sperm concentration (0.34; 0.30; 0.20; 0.07; 0.23) and number of semen portions (0.18; 0.30; 0.04; 0.14; 0.04) in BS, HO, RH, SF and SI breed, respectively. The phenotypic and genetic correlations across all breeds between ejaculate volume and sperm concentration were negative (?0.28; ?0.56). The other correlations across all breeds were positive. The phenotypic and genetic correlations were 0.01 and 0.19 between sperm motility and ejaculate volume, respectively. Between sperm motility and sperm concentration, the phenotypic and genetic correlations were 0.20 and 0.36, respectively. The results are consistent with other analyses and show that genetic improvement through selection is possible in bull semen production traits.  相似文献   

Electroretinography is a prognostic indicator for postoperative vision in dogs undergoing retinal reattachment surgery          下载免费PDF全文

Allison Hoffman  Steve Sisler  Marie Pappania  Kimberly Hsu  Maya Ross  Ron Ofri 《Veterinary ophthalmology》2018,21(3):273-280

  相似文献   

Insecticidal Activity and HPLC Correlation of Thuringiensin from Fermentation and Two-phase Aqueous Separation Processes     

Tai-Hao Hsu  Yew-Min Tzeng  Mei-Mao Wu 《Pest management science》1997,50(1):35-41

Thuringiensin produced by Bacillus thuringiensis subsp. darmstadiensis was further subjected to a two-phase aqueous separation system. A modified HPLC method and a test for quantitative pathogenicity using the house fly Musca domestica were used for analysis of thuringiensin. Within a realistic range of dosages, more effect was observed in the pupal stage than in the larval stage. The percentage effective control rate (ECR) was calculated by (100-percentage emergence); malformed and non-reproductive adults were considered as emerged. Pupal mortality, pupal weight, and ECR after feeding the three-day-old larvae were the measured response criteria for bioassay. The EC₅₀ of thuringiensin for pupae mortality was 1·64 μg ml^-1 diet, and 0·83 μg ml^-1 for mortality of adults. Insecticidal activity of the broth increased with fermentation time-course from 9th to 21st hour. The bioassay curve constructed with three-hour sampling interval during the fermentation course had good correlation to thuringiensin content as determined by the HPLC method. In the two-phase aqueous separation system, a maximum of 96·7% ECR was achieved with the bottom salt layer, compared to a value of 46·7% with the upper PEG layer. These results suggest that thuringiensin, prepared through a fermentation and recovery process, is suitable for pest control. © 1997 SCI.  相似文献   

Modelling the impacts of environmental variation on habitat suitability for Pacific saury in the Northwestern Pacific Ocean     

Yi‐Jay Chang  Kuo‐Wei Lan  William A. Walsh  Jhen Hsu  Chih‐hao Hsieh 《Fisheries Oceanography》2019,28(3):291-304

We developed habitat suitability index (HSI) models for two size classes of Pacific saury Cololabis saira in the Northwestern Pacific Ocean. Environmental data, including sea surface temperature, sea surface height, salinity, and net primary production, and catch and effort data from Taiwanese distant‐water stick‐held dip net fisheries during the main fishing season (August–October) during 2002–2015 were used. Habitat preferences and suitable habitat area differed between size classes. The suitable habitat was located between 40–47.5°N and 145–165°E for large‐sized Pacific saury but encompassed a greater area (35–47°N and 140–165°E) for medium‐sized Pacific saury. Both size classes were affected by substantial interannual variation in the environmental variables, which in turn can be important in determining the potential fishing grounds. We found a significant negative relationship between the suitable habitat area and the Niño3.4 indices with a time‐lag of 6 months for the large‐sized (r = ?0.68) and medium‐sized (r = ?0.42) Pacific saury, respectively, as well as the total landings of Pacific saury by all fishing fleets (r = ?0.46). As remotely‐sensed environmental data become increasingly available, HSI models may prove useful for evaluation of possible changes in habitat suitability resulting from climate change or other environmental phenomena and in formulating scientific advice for management.  相似文献