Sister-chromatid cohesion, established during replication by the protein complex cohesin, is essential for both chromosome segregation and double-strand break (DSB) repair. Normally, cohesion formation is strictly limited to the S phase of the cell cycle, but DSBs can trigger cohesion also after DNA replication has been completed. The function of this damage-induced cohesion remains unknown. In this investigation, we show that damage-induced cohesion is essential for repair in postreplicative cells in yeast. Furthermore, it is established genome-wide after induction of a single DSB, and it is controlled by the DNA damage response and cohesin-regulating factors. We thus define a cohesion establishment pathway that is independent of DNA duplication and acts together with cohesion formed during replication in sister chromatid-based DSB repair. 相似文献
This paper presents a method to separate harvested area and yield for irrigated crops from rainfed crops in a region, given
gross harvested area and yield, and climatic, agronomic and economic data for crops. The method is based on the principle
of general maximum entropy, which combines incomplete data, empirical knowledge and a priori information to derive desired
information. The model is applied to three large basins with aggregated climatic and agricultural conditions, and to five
counties in Texas and California. The modeled results and assessed values in these study areas are compared. While the dependability
of model outputs relies on empirical knowledge and judicious parameter estimation, the model remains reliable even for the
significant level of uncertainty produced by subjectively predetermined major parameters. The model can be applied to retriving
historical data for irrigated and rainfed crops; it can also be used for irrigated and rainfed agriculture planning based
on climatic and technological projections. Moreover, the model provides other useful information, including water allocation
by crop, water use efficiency and the impact of other agricultural inputs. 相似文献
Ferulic acid (FA) cross-links have been implicated in the thermal stability of texture in Chinese water chestnut (CWC) tissues. The aim of the current study has been to investigate this concept further. CWC tissue strips were measured for their mechanical properties before and after extraction in increasing strengths of alkali. The mechanical properties were related to the associated mode of fracture (cell separation or breakage) at the fracture surfaces and the phenolic composition of the cell walls. CWC tissue softened after prolonged extraction in cold alkali due to an increase in the ease of cell separation. Analysis of wall-bound phenolics demonstrated that most FA moieties, including five of the six dehydrodimers, were released before tissue strength was reduced. Loss of strength was, however, coincident with the loss of 8,8'-diferulic acid, aryltetralin (AT) form. It has been suggested that this dehydrodimer may be particularly concentrated at the edge of the cell faces. These results provide further evidence for the involvement of this dehydrodimer in conferring thermal stability of cell-cell adhesion and hence texture in CWC. However, they do not exclude the other diferulates from involvement in cell adhesion. 相似文献
Open space lands are provided by a variety of entities from private individuals to the federal government and these entities make management decisions based on a very broad range of priorities. The net benefits of additional open space depend on the number, quality, and composition of existing open space in the vicinity. In areas where open space is abundant and there is a significant proportion that is federally owned, the net benefits are not well understood. In this study, the marginal willingness to pay for proximity to public open space is estimated using the hedonic property method and home sales transactions in 2007 for El Paso County Colorado, a location that boasts of having over 150,000 acres of open space. Results from a generalized spatial two‐stage least squares regression indicate that homes in close proximity to open space provided locally by city, county, and state governments sell for a premium, but larger premiums are associated with proximity to the Pike National Forest and Cheyenne Mountain Air Force Station likely at least partially due to the natural amenities of the Rocky Mountains. In contrast, proximity to Fort Carson Army Installation is found to negatively affect house sales prices likely due to undesirable activities that occur on and around the installation. As for the other area of federal lands, proximity to the U.S. Air Force Academy and to Peterson Air Force Base was found to negatively affect house prices; however, these effects were not statistically significant. 相似文献
Xylazine is widely used worldwide as a short-acting sedative in general equine and racing practice. In the UK, although it has a legitimate use during training, equine anti-doping rules state it is a prohibited substance on race day. The aim of the study was to produce a detection time (DT) to better inform European veterinary surgeons so that xylazine can be used appropriately under regulatory rules. Previous publications have various limitations pertaining to analysis method, particularly for plasma and limited length of time of sample collection. In this study, pharmacokinetic data were produced for xylazine and 4-OH-xylazine in equine urine and plasma following a single intravenous xylazine dose of 0.4 mg/kg to six Thoroughbred horses. Pharmacokinetic parameters were generated from a 3-compartmental model with clearance = 15.8 ± 4.88 ml min-1 kg-1, Vss = 1.44 ± 0.38 L/kg, terminal half-life = 29.8 ± 12.7 hr and a DT determined at 71 hr for the administration of xylazine (Chanazine®) in plasma and urine. Urine screening should aim to detect the 4-OH-xylazine metabolite, which can act as an indicator for the xylazine plasma concentration. A DT of 72 hr has been agreed by the European Horserace Scientific Liaison Committee, to be implemented in June 2019. 相似文献
Cocoa is a major crop and income source for most farmers and rural households in the Centre, South and South-West regions of Cameroon, where cocoa is generally produced in agroforestry systems. In this country, cocoa-based agroforestry systems (CBAFS) are undergoing multiple changes alongside the rapid changes underway in the natural, economic and socio-political conditions. This study—carried out in the Akongo subregion in central Cameroon—was designed to gain insight into the CBAFS trends and dynamics in the light of those multiple changes. This semi-structured socioeconomic survey involved interviews and direct observations at plot, farm, household and village scales. Overall, forty cocoa growers from ten villages were interviewed and then fifteen cocoa plots were characterized on the basis of the survey findings. They revealed that cocoa was the major crop in this study area, with cocoa plantations occupying three quarters of the total farming area. Three types of CBAFS were identified, which differed according to their vegetation structure, management practices and age of the plantations. Dynamics affecting the structural characteristics and the spatial extension of these systems emerged and were intimately linked to the dynamics of the cocoa farmer population in relation to their context.
The antioxidative effect of purified carnosine (i.e., separated from the common contaminant hydrazine) has been evaluated in two systems: (i) Carnosine was found to possess poor reducing properties toward the prooxidant ferrylmyoglobin; at pH approximately 5 the presence of carnosine did not increase the rate of reduction of MbFe(IV)=O compared to autoreduction, whereas at pH 7.4 the rate constant for reduction by carnosine was 0.010 +/- 0.002 M(-1).s(-1) (I = 0.16; 25.0 degrees C). (ii) In cooked pork patties prepared from meat (longissimus dorsi and masseter) with purified or nonpurified carnosine added, the effect of purified carnosine was insignificant when compared to control patties, whereas patties with carnosine contaminated with hydrazine had a lower oxidation level than patties with purified carnosine. Carnosine is concluded not to deactivate the prooxidant ferrylmyoglobin and not to have any antioxidative effect in cooked pork. 相似文献
The contents of raffinose family oligosaccharides (RFO) and sucrose in Brassica, Lupinus, Pisum, and Hordeum species were investigated by chemometric principal component analysis (PCA). Hordeum samples contained sucrose and raffinose, and Brassica samples all contained sucrose, raffinose, and stachyose. In addition to these, the Pisum samples contained verbascose and the Lupinus samples also contained ajugose. High stachyose and low ajugose contents were found in Lupinus albus in contrast to Lupinus angustifolius, having low stachyose and high ajugose contents. Lupinus luteus had average stachyose and ajugose contents, whereas large amounts of verbascose were accumulated in these seeds. Lupinus mutabilis had high stachyose and low ajugose contents, similar to the composition in L. albus but showing higher raffinose content. The Brassica samples also showed compositional RFO variations within the species, and subgroup formations were discovered within the investigated Brassica napus varieties. PCA results indicated compositional variations between the investigated genera and within the various species of value as chemotaxonomic defined parameters and as tools in evaluations of authenticity/falsifications when RFO-containing plants are used as, for example, feed and food additives. 相似文献
Objective: To compare ultrasound‐guided lateral and medial approaches for periarticular injections of the thoracolumbar intervertebral facet joints (IFJ). Study Design: Experimental cadaveric study. Sample Population: Adult equine cadavers (n=4). Methods: IFJ (T12–T13 to L5–L6) were identified by ultrasound (transducer perpendicular to the spine axis) and insertion of a 13 cm, 18 g spinal needle monitored until bone contact using medial (right side) and lateral (left side) approaches. Number of needle insertions at each site, needle repositioning, and insertion depth were recorded. On bone contact 2 mL latex was injected. Intraarticular deposition, distance of latex from the closest articular margin, and presence of latex in the multifidus muscle were established by dissection. Results: Of 96 attempts, only 1 site require reinsertion of the needle; however, 46% of the injections required needle repositioning. Mean ± SD insertion depth was 8.5 ± 1.1 cm. Most injections (86%) were intraarticular and 96% were at or within 0.5 cm of the closest articular margin. Needle insertion relative to the transducer (lateral, medial) had no effect on the distance from the latex to the closest articular margin and all injections were performed into the multifidus muscle. Conclusions: Ultrasound‐guidance facilitated accurate periarticular injection of thoracolumbar IFJ irrespective of using a medial or lateral approach. 相似文献