Evaluation of Selected Cytokine Gene Expression in Colonic Mucosa from Dogs with Idiopathic Lymphocytic-plasmacytic Colitis     

Yu TAMURA  Hiroshi OHTA  Nozomu YOKOYAMA  Sue Yee LIM  Tatsuyuki OSUGA  Keitaro MORISHITA  Kensuke NAKAMURA  Masahiro YAMASAKI  Mitsuyoshi TAKIGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(10):1407-1410

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Sparing Effect of Robenacoxib on the Minimum Alveolar Concentration for Blunting Adrenergic Response (MAC-BAR) of Sevoflurane in Dogs     

Jun TAMURA  Takaharu ITAMI  Tomohito ISHIZUKA  Sho FUKUI  Norihiko OOYAMA  Kenjiro MIYOSHI  Tadashi SANO  Kazuto YAMASHITA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(1):113-117

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Role of oocyte‐derived paracrine factors in follicular development     

Chihiro Emori  Koji Sugiura 《Animal Science Journal》2014,85(6):627-633

Mammalian oocytes secrete transforming growth factor β (TGF‐β) superfamily proteins, such as growth differentiation factor 9 (GDF9), bone morphogenetic protein 6 (BMP6) and BMP15, and fibroblast growth factors (FGFs). These oocyte‐derived paracrine factors (ODPFs) play essential roles in regulating the differentiation and function of somatic granulosa cells as well as the development of ovarian follicles. In addition to the importance of individual ODPFs, emerging evidence suggests that the interaction of ODPF signals with other intra‐follicular signals, such as estrogen, is critical for folliculogenesis. In this review, we will discuss the current understanding of the role of ODPFs in follicular development with an emphasis on their interaction with estrogen signaling in regulation of the differentiation and function of granulosa cells.  相似文献   

Reductions in greenhouse gas emissions by using wood to protect against soil liquefaction     

Chihiro Kayo  Seiji Hashimoto  Atsunori Numata  Masanori Hamada 《Journal of Wood Science》2011,57(3):234-240

We compared the greenhouse gas (GHG) emissions from a log pile (LP) to those from a sand compaction pile (SCP) and from cement deep mixing (CDM) as measures against soil liquefaction, assuming that forest and waste management scenarios influence the GHG (CO₂, CH₄, and N₂O) balance of wood. We found little difference between the LP and SCP methods with respect to GHG emissions from fossil fuel and limestone consumption. However, GHG emissions from the CDM method were seven times higher than emissions from the LP method. In the GHG balance of wood, when the percentage of CH₄ emissions from carbon in underground wood was lower than 3.3%, permanent storage in the log achieved greater reductions in GHG emissions than using the waste log as fuel in place of coal or heavy oil. In order to obtain reductions in GHG emissions by replacing SCPs or CDM with LPs, sustainable forest management with reforestation and prevention of CH₄ emissions from the underground log are essential. Using reforestation, permanent storage of the log, no CH₄ emission from the log, and using logging residues instead of coal, the LP can achieve reductions in GHG emissions of 121 tonnes of CO₂ per 100 m² of improvement area by replacing CDM.  相似文献   

Methane emissions from five paddy fields with different amounts of rice straw application in central Hokkaido, Japan   总被引：6，自引：1，他引：6  

Habib Mohammad NASER  Osamu NAGATA  Satsuki TAMURA  Ryusuke HATANO 《Soil Science and Plant Nutrition》2007,53(1):95-101

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Effect of dietary addition of seaweed and licorice on the immune performance of pigs     

Masafumi KATAYAMA  Tomokazu FUKUDA  Toshihiro OKAMURA  Eisaku SUZUKI  Katsuo TAMURA  Yuuko SHIMIZU  Yoshihito SUDA  Keiichi SUZUKI 《Animal Science Journal》2011,82(2):274-281

In pig production, dietary additive antibiotics are usually used for growth stimulation and disease prevention, although there is public concern about the increased incidence of resistant antibiotics and food safety. It is possible that such antibiotics might be replaced by naturally derived products such as seaweed and licorice. In this study, we evaluated the effect of dietary addition of seaweed and licorice on enhancing the immune function in swine. The animals of each group (eight animals per group) were sensitized at day 42 and 49, and the immunoglobulin production and the expression of cytokines were detected by the ELISA and real‐time PCR. As the results, saliva IgA production of the seaweed‐treated group increased around five times compared to that of control (day 56). Delayed hypersensitivity reaction and IgG production of the seaweed‐treated group increased around 1.8–2.0 times. In addition, enhanced saliva IgA production was detected at day 50 (around two times) and day 51 (around five times) by the licorice treatment, and lower expression level of tumor necrosis factor‐α messenger RNA at day 51 (around 1/25) was observed in the licorice treatment. We conclude that the replacement of antibiotics by naturally derived dietary additives might be feasible for immune system enhancement.  相似文献   

Molecular epidemiological survey of benign Theileria parasites of cattle in Japan: detection of a new type of major piroplasm surface protein gene     

Kim JY  Yokoyama N  Kumar S  Inoue N  Yamaguchi T  Sentoku S  Fujisaki K  Sugimoto C 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(3):251-256

Benign Theileria species of cattle are found in most parts of the world. The major piroplasm surface protein (MPSP), a conserved protein in all Theileria species, has been used as a maker for epidemiological and phylogenetical studies of benign Theileria species. Parasites with Ikeda- or Chitose-type MPSP genes are dominant in Japan, but we report here mixed infection cases of Theileria parasites with an additional MPSP type parasite infecting cattle in Abashiri District, Hokkaido. The MPSP gene sequence found in the additional type was closely related to MPSP genes of Theileria parasites found in Southeast Asian countries, including Thailand (Narathiwat) and Indonesia (Java). Theileria parasites from the blood sample were also distinguishable from the Ikeda or Chitose type parasites by the small subunit (SSU) rRNA gene sequence analysis, and they are grouped into the SSU rRNA types C/D found in Korea, North America, and Spain. The present finding of mixed infections of cattle with three different types of Theileria makes epidemiological feature of bovine theileriosis in Japan more complex. We have designed a set of primers specific to this MPSP type in order to conduct further epidemiological study.  相似文献   

Immunohistochemical localization of steroidogenic enzymes in the testis of Hokkaido Sika deer (Cervus nippon yesoensis)     

Hayakawa D  Sasaki M  Akabane C  Kitamura N  Tsubota T  Suzuki M  Yamada J 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1463-1466

The testes from 15 adult male Hokkaido Sika deer (Cervus nippon yesoensis) were collected during the rutting season (October and November). We investigated the localization of 4 kinds of steroidogenic enzymes (P450scc, 3betaHSD, P450c17 and P450arom) immunohistochemically in these testicular samples. The specific immunoreactivities to these enzymes were detected only in the cytoplasm of Leydig cells. This differs to the enzyme distributions reported previously in Japanese black bear, Japanese raccoon dog, Hokkaido brown bear and American black bear, in which the same immunoreactivities were detected in Leydig cells, Sertoli cells and/or spermatogenic cells. The current study suggests that in the testes of the Hokkaido Sika deer, testosterone and estradiol-17beta may be synthesized in the Leydig cells only.  相似文献   

PCR-based discrimination of Toxoplasma gondii from pigs at an abattoir in Okinawa, Japan     

Zakimi S  Kyan H  Oshiro M  Sugimoto C  Fujisaki K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(4):401-404

To determine the prevalence of the 3 primary clonal lineages of Toxoplasma gondii (strain types I, II, and III) in pigs in Okinawa Prefecture, we analyzed lymph node samples that had been collected at an abattoir by PCR analysis using primers specific for the Toxoplasma gondii SAG2 locus. This study revealed the presence of this parasite in 57 out of 101 samples examined. Restriction fragment length polymorphism (RFLP) in PCR-amplified SAG2 products was used to group strains into one of the three genotypes of T. gondii. Genotypes I and II were equally predominant, accounting for 22 (44.9%) and 23 (46.9%) of 49 SAG2-positive samples, respectively, while the type III strain was found in only 4 (8.2%) of the 49 samples. The other 8 samples were indistinguishable by PCR-RFLP analysis. Polymorphisms for the 3 genotypes were confirmed at the sequence level for several samples using the sequences from the RH strain, the Beverley strain, and the C56 strain as references. On the other hand, the dihydropteroate synthase gene, which is responsible for sulfonamide resistance, was amplified in 40 of 54 SAG2-positive samples by PCR with the specific primers, and further RFLP and sequence analysis revealed that none of them carried the drug-resistant form of the dhps gene. This is the first report of genotyping of T. gondii distributed in Japan.  相似文献   

The presence of a short form of p53 in chicken lymphoblastoid cell lines during apoptosis     

Takagi M  Takeda T  Asada Y  Sugimoto C  Onuma M  Ohashi K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(6):561-566

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