人工草地土壤水分周年变化规律的研究   总被引：14，自引：0，他引：14  

余优森  林日暖  邓振镛  仇化民 《土壤学报》1992,29(2):175-182

本文利用人工牧草地土壤水分试验测定资料,分析研究了草地土壤水分的周年变化规律,得出草地土壤水分周年变化可划分为春季失墒期、夏季增失墒交替期、伏秋增墒期;土壤水分动态曲线波动主要受降水补给量和牧草耗水量的影响而变化,雨季降水对旱作草地土壤水分的补给起着重要作用。  相似文献   

Purification, cloning, and identification of two thaumatin-like protein isoforms in jelly fig (Ficus awkeotsang) Achenes     

Chua AC  Chou WM  Chyan CL  Tzen JT 《Journal of agricultural and food chemistry》2007,55(18):7602-7608

Jelly curd used for a popular summer drink in Taiwan is prepared by extracting the pericarpial portion of jelly fig (Ficus awkeotsang Makino) achenes. The two most abundant proteins found in jelly curd have been identified as a pectin methylesterase and a chitinase. A method was developed to purify the next abundant protein by 40% ammonium sulfate precipitation and flowing through Mono Q chromatography. In sodium dodecyl sulfate-polyacrylamide gel electrophoresis analyses, the purified protein migrated as a polypeptide of 20 kDa in the absence of beta-mercaptoethanol but split into a minor polypeptide of 20 kDa and a major polypeptide of 27 kDa in the presence of this reducing agent. Two cDNA fragments encoding precursor polypeptides of two putative thaumatin-like protein isoforms were obtained by polymerase chain reaction cloning and subsequently overexpressed in Escherichia coli to generate recombinant proteins for antibody preparations. Immunological detection and mass spectrometric analyses indicated that the two split polypeptides were thaumatin-like protein isoforms encoded by the two cloned cDNA fragments.  相似文献   

雪花梨贮藏中红肉病的研究   总被引：5，自引：1，他引：4  

陈雨新  刘兴民 《园艺学报》1993,20(1):8-12

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电取蜂毒对中华蜜蜂寿命的影响   总被引：4，自引：0，他引：4  

周冰峰  张富荣 《中国蜂业》1993,(2):4-6

采用QF—1型电子取毒仪,分别电刺激3～27日龄的中蜂工蜂5分钟,结果3和6日龄的工蜂寿命缩短差异不显著(P>0.05);9、12、15、18日龄的工蜂寿命缩短2.75～4.59天,差异显著(P<0.05);21、24、27日龄的工蜂寿命缩短4.50～4.75天,差异极显著(P<0.01)。  相似文献   

Proportion and cluster analyses of the skull in various species of the tree shrews     

Endo H  Hikida T  Chou LM  Fukuta K  Stafford BJ 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(1):1-7

The skull adaptation was functional-morphologically examined in 14 species of the tree shrews. From the data of the proportion indices, the similarities were confirmed between T. minor and T. gracilis, T. tana and T. dorsalis, and T. longipes and T. glis. We demonstrated that the splanchnocranium was elongated in terrestrial T. tana and T. dorsalis and shortened in arboreal T. minor and T. gracilis from the proportion data. In both dendrogram from the matrix of the Q-mode correlation coefficients and scattergram from the canonical discriminant analysis, the morphological similarities in the skull shape suggested the terrestrial-insectivorous adaptation of T. tana and T. dorsalis, and the arboreal adaptation of T. minor and T. gracilis. Since the osteometrical skull similarities were indicated among the three species of Tupaia by cluster and canonical discriminant analyses, the arbo-terrestrial behavior and its functional-morphological adaptation may be commonly established in T. montana, T. longipes and T. glis.  相似文献   

Bovine ephemeral fever in Taiwan (2001-2002)   总被引：3，自引：0，他引：3  

Hsieh YC  Chen SH  Chou CC  Ting LJ  Itakura C  Wang FI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2005,67(4):411-416

Bovine ephemeral fever (BEF), a vector-borne disease of cattle, is caused by the Ephemerovirus of the family Rhabdoviridae. In the past 40 years, Taiwan has had seven BEF epizootics, and we have previously reported the first five. This study summarizes the 2001 and 2002 epizootics; conducted case-control serologic studies on 10 herds involved in the 2001 epizootic; determined whether the recent BEF viruses have varied significantly; and discusses the relationship between epizootic patterns and possible variant BEF viruses. For mature cows that had received at least 2 doses of vaccine before the study, a negative correlation between the prevaccinated (the 3rd dose and after) serum neutralization antibody (SNA) titers and their postvaccinated peak rates was found. When prevaccinated SNA levels were at < or = 32, their postvaccinated SNA levels increased significantly faster (P<0.01) than for those at > or = 32. The glycoprotein gene of isolates from 1999, 2001, and 2002 had a 99.2-99.9% homology, without consistent amino acid variations in the neutralization sites. Phylogenetic analysis of Taiwanese isolates revealed 2 distinct clusters, the 1983-1989 and 1996-2002 isolates. Cross-neutralization tests confirmed the glycoprotein gene sequence analysis results. In conclusion, annual boosters at SNA levels > 32, at more than 2 doses, or at intervals shorter than 6 months are not advisable. The occurrence of frequent small epizootics implies the dominance of BEF virus over host immunity, but not a variant virus.  相似文献   

溴甲烷熏蒸马铃薯种薯防治块茎蛾的研究   总被引：2，自引：0，他引：2  

钱念曾  周长初  郑文钻  屠乐平 《植物保护学报》1965,4(3):237-248

溴甲烷熏蒸馬鈴薯种薯防治块茎蛾研究結果:在温度10—15℃和15—20℃,药量每立方米35克;温度7—10℃,每立方米45克,密閉均为3小时,对蛹、幼虫、卵、成虫的死亡率均为100％。在温度20℃,药量每立方米45克,密閉5小时,在职上熏蒸因子幅度范围职内,相隔5天后播种,对出苗、生长和产量均无不夏影响。休眠、有芽或淋雨种薯熏蒸后,其出苗、生长亦无显著差異。各虫态致死中率測定:以中期蛹耐药力最强,順序为卵、幼虫和成虫。中期蛹的药量,比卵高2倍,比幼虫高4倍左右,比成虫高8倍。成虫致死中率药量只需每升4毫克,与溴甲烷木箱熏蒸結果是相符合的。在大規模熏蒸示范中,得出一套仓庫熏蒸和帐幕熏蒸的操作經驗,可供生产上应用。  相似文献   

辽宁地区玉米螟化学防治的研究   总被引：2，自引：0，他引：2  

忻亦芬  孙承钧  彭元发  金日新  曹尔蕴 《植物保护学报》1964,3(3):299-306

1958—1962年在辽宁省新金、辽中及沈阳三地,对为害玉米及谷子的玉米螟进行了化学防治試驗。試驗証明:玉米心叶末期或田間初見卵后20—25天,或田間第一代螟卵接近绝跡时均可作为辽宁地区进行化学防治适期的标准。用5％DDT或1％六六六顆粒剂适期施药,对第一代玉米螟都有100％效果。于玉米收获前剖查,經5％DDT与1％六六六顆粒剂处理后,減虫率分別为70％以上和60％以上。心叶期增加施药次数并不能增加防治效果。从顆粒剂的加工方法来看,混和顆粒剂优于拌粉顆粒剂。谷子上化学防治的适宜时期,为田间初見卵后15—20天,相当于第一代卵高峯或卵高峯后3—5天。用5％DDT、0.5％六六六1∶1混合粉剂,每亩4斤施于谷子茎基部,一次施药,可得70—80％以上的防治效果。  相似文献   

Detection of the Severe Acute Respiratory Syndrome‐Related Coronavirus and Alphacoronavirus in the Bat Population of Taiwan          下载免费PDF全文

Y.‐N. Chen  V. N. Phuong  H. C. Chen  C.‐H. Chou  H.‐C. Cheng  C.‐H. Wu 《Zoonoses and public health》2016,63(8):608-615

Bats have been demonstrated to be natural reservoirs of severe acute respiratory syndrome coronavirus (SARS CoV) and Middle East respiratory syndrome (MERS) CoV. Faecal samples from 248 individuals of 20 bat species were tested for partial RNA‐dependent RNA polymerase gene of CoV and 57 faecal samples from eight bat species were tested positive. The highest detection rate of 44% for Scotophilus kuhlii, followed by 30% for Rhinolophus monoceros. Significantly higher detection rates of coronaviral RNA were found in female bats and Scotophilus kuhlii roosting in palm trees. Phylogenetic analysis classified the positive samples into SARS‐related (SARSr) CoV, Scotophilus bat CoV 512 close to those from China and Philippines, and Miniopterus bat CoV 1A‐related lineages. Coronaviral RNA was also detected in bat guano from Scotophilus kuhlii and Myotis formosus flavus on the ground and had potential risk for human exposure. Diverse bat CoV with zoonotic potential could be introduced by migratory bats and maintained in the endemic bat population in Taiwan.  相似文献   

CD205-positive,Sepharose-induced peritoneal exudate cells: a new resource for DC research in the chicken     

Vuong  Christine N.  Chou  Wen-Ko  Berghman  Luc R. 《Veterinary research communications》2019,43(2):115-122

Veterinary Research Communications - Dendritic cells (DC) are important antigen-presenting cells and are among the least characterized immune cells in the chicken. In order to obtain chicken DC,...  相似文献