Density,metabolic activity,and identity of cultivable rhizosphere bacteria on Salix viminalis in disturbed arable and landfill soils     

Katarzyna Hrynkiewicz  Christel Baum  Peter Leinweber 《植物养料与土壤学杂志》2010,173(5):747-756

The objective of this study was to provide fundamental data for a subsequent selection of willow growth and soil remediation promoting bacterial strains. The rhizosphere of willows (Salix viminalis) was screened for cultivable bacteria with high enzymatic activity (proteolytic, pectolytic, cellulolytic, amylolytic) and production of siderophores at four test sites with broad spectrum of anthropogenic soil disturbance: sewage‐sludge application, impoverishment by unfavorable arable use, ash dumping, and household‐waste depositing. The density of bacteria in the rhizosphere ranged from 7.92 to 8.56 log₁₀ of colony‐forming units per gram dry weight of soil and varied in a site‐ and willow‐clone‐specific manner. Within the 240 bacterial strains, a high diversity of metabolic activities was observed but was rarely combined in one strain (1.2% having six and 5.8% having five out of seven metabolic activities, respectively). The majority of strains (79.2%) revealed just one or two metabolic activities. Most common was a combination of lipolytic, proteolytic activities, and siderophore production as found in 13.8% of the bacterial strains. The 50 strains with the highest metabolic activity belonged predominantly to the Gammaproteobacteria (66%), the others to Flavobacteria (18%), Betaproteobacteria (8%), Actinobacteria (4%), and Bacilli (4%). The highest portion of cultivable strains of rhizosphere bacteria with high metabolic activities belonged to the genera Pseudomonas, Serratia, and Flavobacterium. We hypothesize that these genera include strains that support willow growth and soil remediation. Therefore, the described strain collection from the rhizosphere of S. viminalis provides a valuable basis for a subsequent selection of these candidates for applications in improvement of site adaptation of plants or remediation of soils.  相似文献   

Development of a seven-target multiplex PCR for the simultaneous detection of transgenic soybean and maize in feeds and foods   总被引：16，自引：0，他引：16  

Germini A  Zanetti A  Salati C  Rossi S  Forré C  Schmid S  Marchelli R  Fogher C 《Journal of agricultural and food chemistry》2004,52(11):3275-3280

The detection of genetically modified organisms (GMOs) in food and feed is an important issue for all the subjects involved in raw material control, food industry, and distribution. Because the number of GMOs authorized in the EU increased during the past few years, there is a need for methods that allow a rapid screening of products. In this paper, we propose a method for the simultaneous detection of four transgenic maize (MON810, Bt11, Bt 176, and GA21) and one transgenic soybean (Roundup Ready), which allows routine control analyses to be sped up. DNA was extracted either from maize and soybean seeds and leaves or reference materials, and the recombinant DNA target sequences were detected with 7 primer pairs, accurately designed to be highly specific for each investigated transgene. Cross and negative controls were performed to ensure the specificity of each primer pair. The method was validated on an interlaboratory ring test and good analytical parameters were obtained (LOD = 0.25%, Repeatability, (r) = 1; Reproducibility, (R) = 0.9). The method was then applied to a model biscuit made of transgenic materials baked for the purpose and to real samples such as feed and foodstuffs. On account of the high recognition specificity and the good detection limits, this multiplex PCR represents a fast and reliable screening method directly applicable in all the laboratories involved in raw material and food control.  相似文献   

Wirkungen technogener Druckbelastung auf die Spannungsverteilung und das Bodengefüge einer Schwarzerde aus Löß     

Rainer Horn  Dietrich Werner  Thomas Baumgartl  Christel Winterot 《植物养料与土壤学杂志》1994,157(6):433-440

Effect of wheeling on stress distribution and changes in the macro- and microstructure of a cambic phaeozem derived from loess The determination of stress induced changes in the poresystem due to repeated wheeling of a cambic phaeozem derived from loess as well as in the aggregate size distribution, arrangement, and stage are reported. It could be shown, that repeated wheeling always results in a further stress distribution to deeper depths and that the vertical stresses dominate with repeated wheeling. Furthermore the octahedral shear stresses and mean normal stresses exceed the maximum strength defined by the Mohr Coulomb failure line and define a further soil deformation. The process of aggregate deterioration can be verified by radiographs from which not only the changes in bulk density on a mesoscale can be derived but from which also the crack patterns can be defined for the different stages of structure deterioration. Under the well known assumption that pore water pressure is increased due to wheeling, soil compaction and consecutive soil homogenisation by kneading can be differentiated. The consequences for ecological parameters will be discussed.  相似文献   

Darstellung des Bodengefüges durch Röntgenanalyse     

Dietrich Werner  Christel Winterot  Barbara Werner  Kurt John 《植物养料与土壤学杂志》1993,156(5):427-434

Characterization of soil structure by X-ray analysis Frequently the use of morphological observation of soil structure to assess effects of tillage operations has been restricted by the extreme slowness of sample preparation. A method for rapid analysis of structure with the help of X-ray radiographs is described. The method is based on differences in attenuation of transmitted X-rays by soil pores and the adjoining soil mass. After digitizing X-ray pictures of unimpregnated soil cores using a video scanner and digitizer we found a close relationship between the average bulk density of soil within a core and the mean greylevel value of the digital image when sample thickness, origin and organic matter content of soil material, energy of radiation and sensitivness of X-ray film are the same. Application of a universal image processing system (DIAS) for the quantification of image parameters by procedures of binarization, skeletonization and direction coding demonstrated that radiographs are useful for analyzing spatial distribution, shape and orientation of soil structure elements. Results are illustrated by examples of soil tillage experiments.  相似文献   

Modeling combined continuous and ordinal outcomes in a clustered setting     

Christel?FaesEmail author  Helena?Geys  Marc?Aerts  Geert?Molenberghs  Paul?J.?Catalano 《Journal of Agricultural, Biological & Environmental Statistics》2004,9(4):515-530

Measurements of both continuous and categorical outcomes appear in many statistical problems. One such example is the study of teratology and developmental toxicity, where both the probability that a live fetus is malformed (ordinal) or of low birth weight (continuous) are important measures in the context of teratogenicity. Although multivariate methods of the analysis of continuous outcomes are well understood, methods for jointly continuous and discrete outcomes are less familiar. We propose a likelihood-based method that is an extension of the Plackett-Dale approach. Specification of the full likelihood will be avoided using pseudo-likelihood methodology. The estimation of safe dose levels as part of quantitative risk assessment will be illustrated based on a developmental toxicity experiment of diethylene glycol dimethyl ether in mice.  相似文献   

Erfassung der Variabilität von Bodenmerkmalen in einem Blindversuch auf Fahlerde mittels Methoden der räumlichen Statistik     

Irina Kuzyakova  Christel Richter 《植物养料与土壤学杂志》2003,166(3):348-356

Variability of soil parameters in a uniformity trial on a Luvisol evaluated by means of spatial statistics The relationships between soil parameters and indicators of soil genesis were investigated in a uniformity trial on Luvisol by means of classical and spatial statistics. The following properties were investigated in the topsoil (0–30 cm) and subsoil (31–40 cm): content of total carbon (C_t), content of clay plus fine silt (FAT), concentrations of available potassium (K) and phosphorus (P), and pH values. The depth loamy Bt horizon and microrelief were considered as indicators of soil genesis. Smoothing of the raw data by means of block kriging eliminated the small scale variability of all parameters. In this way, the rank correlation coefficients between soil parameters increased by factor 2 and were to 0.52 for C_t–FAT; 0.69 for C_t–K, and 0.55 for C_t–P in the top soil. The maps for smoothed data allowed a much better visual analysis of spatial distribution of investigated properties compared to the raw data. The significant correlations as well as the similarity of maps and variograms suggested that the spatial variability of FAT, C_t, K, and P in the top soil was determined by the same factors. The depth of the loamy Bt horizon and the relief were the most important factors defining the distribution of the investigated soil parameters. The rank correlation coefficients of the smoothed and detrended relief with the soil parameters amount to –0.63, –0.77, –0.71, and –0.57 for FAT, C_t, K, and P, respectively.  相似文献   

Clonal and seasonal shifts in communities of saprotrophic microfungi and soil enzyme activities in the mycorrhizosphere of Salix spp.     

Christel Baum  Katarzyna Hrynkiewicz 《植物养料与土壤学杂志》2006,169(4):481-487

The species‐specific microbial root and rhizosphere colonization contributes essentially to the plant nutrient supply. The species number and colonization densities of cultivable saprotrophic microfungi and the activities of nutrient‐releasing soil enzymes (protease, acid and alkaline phosphatase, arylsulfatase) were investigated in the rhizosphere of one low mycorrhizal (Salix viminalis) and one higher mycorrhizal (S. × dasyclados) willow clone at a Eutric Cambisol in N Germany. After soil washing, in total 32 and 28 saprotrophic microfungal species were isolated and identified microscopically from the rhizosphere of S. viminalis and S. × dasyclados, respectively. The fungal species composition changed within the growing season but the species number was always lower under S. × dasyclados than under S. viminalis. Under both willow clones, the fungal colonization density was largest in spring, and the species number was largest in autumn. Acid‐phosphatase activity (p < 0.001) and protease activity (p < 0.003) were significantly affected by the Salix clone, whereas arylsulfatase and alkaline‐phosphatase activities did not show clone‐specific differences. All enzyme activities reached their maxima in the summer sampling. Rhizosphere colonization with Acremonium butyri, Cladosporium herbarum, and Penicillium janthinellum contributed significantly to explain the activities of acid phosphatase. Rhizosphere colonization with Cylindrocarpon destructans, Penicillium spinulosum, Plectosphaerella cucumerina, and Trichoderma polysporum contributed significantly to explain the arylsulfatase activities. Effects of the saprotrophic fungal colonization densities on the protease activities in the rhizosphere were low. Acid‐ and alkaline‐phosphatase and arylsulfatase activities in the rhizosphere soil were stronger affected by the composition of the saprotrophic fungal communities than by the Salix clone itself. In conclusion, the colonization density of some saprotrophic microfungi in the rhizosphere contributed to explain shifts in soil‐enzyme activities of the P and S cycles under different willow clones.  相似文献   

Research needs in forest tree breeding and improvement in developing countries     

Christel Palmberg 《Agroforestry Systems》1989,9(1):29-35

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Fine root dynamics in a developing Populus deltoides plantation     

Kern CC  Friend AL  Johnson JM  Coleman MD 《Tree physiology》2004,24(6):651-660

A closely spaced (1 x 1 m) cottonwood (Populus deltoides Bartr.) plantation was established to evaluate the effects of nutrient availability on fine root dynamics. Slow-release fertilizer (17:6:12 N,P,K plus micronutrients) was applied to 225-m(2) plots at 0, 50, 100 and 200 kg N ha(-1), and plots were monitored for two growing seasons. Fine root production, mortality, live root standing crop and life span were analyzed based on monthly minirhizotron observations. Fine root biomass was measured in soil cores. Fine root dynamics were controlled more by temporal, depth and root diameter factors than by fertilization. Cumulative fine root production and mortality showed strong seasonal patterns; production was greatest in the middle of the growing season and mortality was greatest after the growing season. Small diameter roots at shallow soil depths cycled more rapidly than larger or deeper roots. The strongest treatment effects were found in the most rapidly cycling roots. The standing crop of live roots increased with fertilizer treatment according to both minirhizotron and soil coring methods. However, production and mortality had unique treatment response patterns. Although cumulative mortality decreased in response to increased fertilization, cumulative production was intermediate at 0 kg N ha(-1), lowest with 50 kg N ha (-1), and highest with 200 kg N ha(-1). Aboveground growth responded positively to fertilization up to an application rate of 50 kg N ha(-1), but no further increases in growth were observed despite a threefold increase in application rate. Median fine root life span varied from 307 to over 700 days and increased with depth, diameter and nutrient availability.  相似文献   

Ephestia cautella (Walker) als Schädling an Schweinehäuten und Haarschaffellen aus Brasilien     

Christel Knoche 《Journal of pest science》1961,34(9):141-142

Ohne Zusammenfassung Die vorliegende Arbeit wurde durch eine Herrn Prof. Dr. H. Weidner vom Forschungsrat bei der Beh?rde für Wirtschaft und Verkehr der Freien und Hansestadt Hamburg gew?hrte Forschungsbeihilfe erm?glicht. Dem Forschungsrat sei auch an dieser Stelle für die Unterstützung der Untersuchungen an Materialsch?dlingen ergebenst gedankt.  相似文献