全文获取类型
收费全文 | 763篇 |
免费 | 53篇 |
国内免费 | 3篇 |
专业分类
林业 | 43篇 |
农学 | 23篇 |
基础科学 | 2篇 |
182篇 | |
综合类 | 174篇 |
农作物 | 30篇 |
水产渔业 | 57篇 |
畜牧兽医 | 253篇 |
园艺 | 10篇 |
植物保护 | 45篇 |
出版年
2023年 | 4篇 |
2021年 | 13篇 |
2020年 | 13篇 |
2019年 | 15篇 |
2018年 | 15篇 |
2017年 | 15篇 |
2016年 | 24篇 |
2015年 | 23篇 |
2014年 | 18篇 |
2013年 | 26篇 |
2012年 | 38篇 |
2011年 | 38篇 |
2010年 | 26篇 |
2009年 | 16篇 |
2008年 | 33篇 |
2007年 | 32篇 |
2006年 | 26篇 |
2005年 | 45篇 |
2004年 | 32篇 |
2003年 | 23篇 |
2002年 | 29篇 |
2001年 | 29篇 |
2000年 | 26篇 |
1999年 | 25篇 |
1998年 | 17篇 |
1997年 | 7篇 |
1996年 | 8篇 |
1995年 | 13篇 |
1994年 | 8篇 |
1993年 | 12篇 |
1992年 | 15篇 |
1991年 | 11篇 |
1990年 | 17篇 |
1989年 | 13篇 |
1988年 | 14篇 |
1987年 | 8篇 |
1986年 | 6篇 |
1985年 | 13篇 |
1984年 | 10篇 |
1981年 | 5篇 |
1979年 | 5篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1976年 | 5篇 |
1975年 | 4篇 |
1974年 | 7篇 |
1973年 | 4篇 |
1972年 | 4篇 |
1971年 | 3篇 |
1963年 | 3篇 |
排序方式: 共有819条查询结果,搜索用时 15 毫秒
121.
122.
K. L. Hepworth‐Warren B. L. Hay Kraus D. M. Wong A. C. Krull G. L. Metcalf 《Equine Veterinary Education》2017,29(11):603-608
This report describes an 11‐year‐old Percheron mare that presented with signs of colic and was ultimately diagnosed with bacterial peritonitis of unknown origin. Bacterial culture of a peritoneal fluid sample isolated 2 Clostridial species, one of which was strongly suspected to be Clostridium haemolyticum. The horse was markedly hypoalbuminaemic at presentation, leading to the development of low oncotic pressure and ventral oedema. The mare was administered a low molecular weight/low molar substitution hydroxyethyl starch solution in conjunction with other therapies that resulted in marked improvement of clinical signs. The purpose of this report is to describe the clinical findings associated with equine peritonitis associated with C. haemolyticum, a rarely identified pathogen in the horse. Secondly, this report serves to describe the beneficial effects of tetrastarch administration in a clinical case with severe hypoalbuminaemia and ventral oedema. 相似文献
123.
Modal testing based on the theory of transverse vibration of orthotropic plate has shown great potentials in measuring elastic constants of panel products. Boundary condition (BC) and corresponding calculation method are key in affecting its practical application in terms of setup implementation, frequency identification, accuracy and calculation efforts. To evaluate different BCs for non-destructive testing of wood-based panels, three BCs with corresponding calculation methods were investigated for measuring their elastic constants, namely in-plane elastic moduli (E x , E y ) and shear modulus (G xy ). As a demonstration of the concept, the products used in this study were oriented strand board (OSB) and medium density fiberboard (MDF). The BCs and corresponding calculated methods investigated were, (a) all sides free (FFFF) with one-term Rayleigh frequency equation and finite element modeling, (b) one side simply supported and the other three free (SFFF) with one-term Rayleigh frequency equation, (c) a pair of opposite sides along minor strength direction simply supported and the other pair along major strength direction free (SFSF) with improved three-term Rayleigh frequency equation. Differences between modal and static results for different BCs were analyzed for each case. Results showed that all three modal testing approaches could be applied for evaluation of the elastic constants of wood-based panels with different accuracy levels compared with standard static test methods. Modal testing on full-size panels is recommended for developing design properties of structural panels as it can provide global properties. 相似文献
124.
This study is aimed at assessing the ability of two endophytic bacteria originally isolated from healthy oil palm roots, Pseudomonas sp. (UPMP3) and Burkholderia sp. (UPMB3) to induce resistance in susceptible Berangan banana against Fusarium oxysporum race 4 (FocR4). Increased accumulation of resistance-related enzymes such as peroxidase (PO), phenylalanine ammonia lyase (PAL), lignithioglycolic acid (LTGA), and pathogenesis-related (PR) proteins (ehitinase and β-1,3-glucanase) has been observed in plantlets treated with endophytic bacteria UPMP3 and UPMB3 singly or as mixture under glasshouse conditions. Pre-inoculation of banana plantlets with UPMP3 showed a significant reduction in Fusarium wilt incidence 72 d after challenged inoculation with FocR4. UPMB3 was less effective in suppressing Fusarium wilt compared to UPMP3, whereas, the mixture of both endophytes showed an intermediate effect. Based on these results, it is concluded that UPMP3 could be a promising biological control agent that can trigger resistance against Fusarium wilt in susceptible Berangan banana. 相似文献
125.
126.
127.
J Bingham RA Lunt DJ Green KR Davies V Stevens FYK Wong 《Australian veterinary journal》2010,88(6):204-210
Objective To study the potential role of an Australian corvid, the little raven (Corvus mellori), in the surveillance for exotic West Nile virus (WNV) in Australia. Method In a series of trials, little ravens were infected with WNV (strain 4132 New York 1999) and Kunjin virus (strain K42886) by the intramuscular route. They were observed for 20 days during which blood and swab samples were taken for virus isolation. Tissue samples were taken from ravens humanely killed during the acute infection period, and at the termination of the trials, for virus isolation, histopathology and immunohistochemistry. Results Ravens infected with WNV became mildly ill, but all recovered and seroconverted. Blood virus titres peaked around 3 to 4 days after inoculation at levels between 103.0 to 107.5 plaque forming units/mL. Virus or viral antigen was detected in spleen, liver, lung, kidney, intestine, testis and ovary by virus isolation and/or immunohistochemistry. WNV was detected in oral and cloacal swabs from 2 to 7 days post inoculation. The molecular and pathogenic characteristics of the inocula were consistent with them being of high virulence, as expected for this isolate. Ravens infected with Kunjin virus developed viraemia and seroconverted, although they did not develop disease. Conclusions Little ravens do not develop severe disease in response to virulent WNV infection and for this reason may not be important sentinel hosts in the event of an outbreak of WNV, as in North America. However, as they have relatively high viraemias, they may be able to support virus cycles. 相似文献
128.
Schirawski J Mannhaupt G Münch K Brefort T Schipper K Doehlemann G Di Stasio M Rössel N Mendoza-Mendoza A Pester D Müller O Winterberg B Meyer E Ghareeb H Wollenberg T Münsterkötter M Wong P Walter M Stukenbrock E Güldener U Kahmann R 《Science (New York, N.Y.)》2010,330(6010):1546-1548
Biotrophic pathogens, such as the related maize pathogenic fungi Ustilago maydis and Sporisorium reilianum, establish an intimate relationship with their hosts by secreting protein effectors. Because secreted effectors interacting with plant proteins should rapidly evolve, we identified variable genomic regions by sequencing the genome of S. reilianum and comparing it with the U. maydis genome. We detected 43 regions of low sequence conservation in otherwise well-conserved syntenic genomes. These regions primarily encode secreted effectors and include previously identified virulence clusters. By deletion analysis in U. maydis, we demonstrate a role in virulence for four previously unknown diversity regions. This highlights the power of comparative genomics of closely related species for identification of virulence determinants. 相似文献
129.
Numerous types of equine herpesviruses (EHV) continue to afflict horses resulting in a variety of clinical manifestations. While many of the clinical manifestations of EHV are self‐limiting or require only supportive care, some clinical expressions of EHV infections cause severe risk to the horse's overall health and can result in abortion, long‐term deficits or death. Antiviral medications are infrequently utilised therapeutics in equine medicine and their exact efficacy is largely unknown. However, the use of antiviral medications may potentially decrease convalescent time and improve outcome in horses with EHV‐related diseases. Thus, equine practitioners should consider the potential use of antiviral medications in the future. The purpose of this article is to familiarise the equine practitioner with current information in regard to antiviral medications and their potential uses in equine medicine. 相似文献
130.
Chang SC Chen HF Chou MH Wang HC Su HY Wong ML 《Veterinary and comparative oncology》2010,8(4):302-309
Four types of globins for oxygen transport are known in vertebrates, and the haemoglobin is responsible for carrying oxygen in blood. In this study, we found that haemoglobin was also expressed in canine mammary glands. Samples were taken from 26 malignant mammary tumors, 16 normal mammary glands and 10 other normal tissues. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), immunoblotting and mass spectrometry were used to investigate haemoglobin in mammary tissues. The results indicated that normal canine mammary glands expressed high levels of haemoglobin protein as shown by Coomassie blue staining. The identity of haemoglobin was confirmed by immunoblotting and mass spectrometry, and the mass spectrometry data revealed that both alpha-haemoglobin and beta-haemoglobin were expressed. Relative to normal mammary glands, the levels of haemoglobin expression in mammary tumors were lower. Our results also indicated that the haemoglobin was endogenously produced in mammary gland tissues and was not derived from the erythroid cells. 相似文献