EFFECT OF METHYLCELLULOSE ON UPPER GASTROINTESTINAL QUALITY IN DOGS     

Joohyun  Jung  DVM  MS  Mincheol  Choi  DVM  PHD  Jinhwa  Chang  DVM  Sungjoon  Won  DVM  Woqjo  Chung  DVM  Hojung  Choi  DVM  MS  Kichang  Lee  DVM  MS  Junghee  Yoon  DVM  PHD  Hyunkwon  Ha  MD  PHD 《Veterinary radiology & ultrasound》2003,44(6):642-645

This study was performed to evaluate and optimize a small bowel contrast technique using barium and methylcellulose in dogs. Ten healthy dogs underwent both a conventional upper gastrointestinal study that used only barium and a modified study that used barium and methylcellulose. The control group received 10 mL/kg of 40% barium suspension. Experimental groups were divided into three subgroups given 15 mL/kg of different viscosities (low, moderate, and high viscosity) of 0.5% methylcellulose after 4 mL/kg of 40% barium suspension. Compared with the control group, dogs receiving methylcellulose had higher-quality upper gastrointestinal studies. Moderate viscosity of methylcellulose was superior to the other methylcellulose groups. In conclusion, the use of methylcellulose is a simple and effective method for improving the image quality in an upper GI examination.  相似文献   

Effect of dietary energy source on in vitro substrate utilization and insulin sensitivity of muscle and adipose tissues of Angus and Wagyu steers     

Rhoades RD  Sawyer JE  Chung KY  Schell ML  Lunt DK  Smith SB 《Journal of animal science》2007,85(7):1719-1726

Angus (n = 8; 210 kg of BW) and 7/8 Wagyu (n = 8; 174 kg of BW) steers were used to evaluate the effects of dietary energy source on muscle and adipose tissue metabolism and insulin sensitivity. Steers were assigned to either a grain-based (corn) or hay-based (hay) diet and fed to similar final BW. At slaughter, LM and s.c. and i.m. adipose tissue samples were collected. Portions of the LM and adipose tissues were placed immediately in liquid N for later measurement of glycolytic intermediates. Fresh LM and s.c. and i.m. adipose tissues were incubated with [U-(14)C]glucose to assess glucose metabolism in vitro. All in vitro measures were in the presence of 0 or 500 ng/mL of insulin. Also, s.c. and i.m. adipose tissues were incubated with [1-(14)C]acetate to quantify lipid synthesis in vitro. Glucose-6-phosphate and fructose-6-phosphate concentrations were 12.6- and 2.4-fold greater in muscle than in s.c. and i.m. adipose tissues, respectively. Diet did not affect acetate incorporation into fatty acids (P = 0.86). Insulin did not increase conversion of glucose to CO(2), lactate, or total lipid in steers fed hay but caused an increase (per cell) of 97 to 110% in glucose conversion to CO(2), 46 to 54% in glucose conversion to lactate, and 65 to 160% in glucose conversion to total lipid content in adipose tissue from steers fed corn. On a per-cell basis, s.c. adipose tissue had 37% greater glucose oxidation than i.m. adipose (P = 0.04) and 290% greater acetate incorporation into fatty acids than i.m. adipose (P = 0.04). Insulin addition to s.c. adipose tissue from corn-fed steers failed to stimulate glucose incorporation into fatty acids, but exposing i.m. adipose tissue from corn-fed steers to insulin resulted in a 165% increase in glucose incorporation into fatty acids. These results suggest that feeding hay limited both glucose supply and tissue capacity to increase glucose utilization in response to insulin without altering acetate conversion to fatty acids. Because s.c. adipose tissue consistently utilized more acetate and oxidized more glucose than did i.m. adipose, these results suggest that hay-based diets may alter i.m. adipose tissue metabolism with less effect on s.c. adipose tissue.  相似文献   

Lipogenesis and stearoyl-CoA desaturase gene expression and enzyme activity in adipose tissue of short- and long-fed Angus and Wagyu steers fed corn- or hay-based diets   总被引：1，自引：0，他引：1  

Chung KY  Lunt DK  Kawachi H  Yano H  Smith SB 《Journal of animal science》2007,85(2):380-387

Angus and Wagyu steers consuming high-roughage diets exhibit large differences in adipose tissue fatty acid composition, but there are no differences in terminal measures of stearoyl-CoA desaturase (SCD) activity or gene expression. Also, adipose tissue lipids of cattle fed corn-based diets have greater MUFA:SFA ratios than cattle fed hay-based diets. We hypothesized that any changes in SCD gene expression and activity would precede similar changes in adipose tissue lipogenesis between short- and long-fed endpoints. Furthermore, changes in SCD activity and gene expression between production endpoints would differ between corn- and hay-fed steers and between Wagyu and Angus steers. Angus (n = 8) and Wagyu (n = 8) steers were fed a corn-based diet for 8 mo (short-fed; 16 mo of age) or 16 mo (long-fed; 24 mo of age), whereas another group of Angus (n = 8) and Wagyu (n = 8) steers was fed a hay-based diet for 12 mo (short-fed; 20 mo of age) or 20 mo (long-fed; 28 mo of age) to match the end point BW of the corn-fed steers. Acetate incorporation into lipids in vitro was greater (P < 0.01) in corn-fed steers than in hay-fed steers and tended (P = 0.06) to be greater in Wagyu than in Angus s.c. adipose tissue because the rate in Wagyu was twice that of Angus adipose tissue in the corn-fed, short-fed steers. There were diet x end point interactions for lipogenesis in i.m. and s.c. adipose tissues (both P < 0.01) because lipogenesis was 60 to 90% lower in the long-fed cattle than in short-fed cattle fed the corn-based diet. The greatest SCD enzyme activity in Angus s.c. adipose tissue was observed at 24 mo of age (corn-based diet), but activity in Wagyu adipose tissue was greatest at 28 mo of age (hay-based diet; breed x diet x end point interaction, P = 0.08). For short- vs. long-fed endpoints in Angus, s.c. adipose tissue SCD activity was less (hay diet) or the same (corn diet). Conversely, SCD gene expression was greatest in long-fed Wagyu steers fed the hay- or corn-based diets (breed x end point interaction; P < 0.01). Contrary to our hypotheses, SCD activity increased over time, whereas lipogenesis from acetate decreased. However, the developmental pattern of SCD gene expression and activity differed markedly between hay-fed Angus and Wagyu adipose tissues, which may explain the differences in the MUFA:SFA ratios observed in adipose tissues from these cattle.  相似文献   

The Exploitation of Crop Allelopathy in Sustainable Agricultural Production   总被引：10，自引：0，他引：10  

T. D. Khanh  M. I. Chung  T. D. Xuan  S. Tawata 《Journal of Agronomy and Crop Science》2005,191(3):172-184

Crop allelopathy may be useful to minimize serious problems in the present agricultural production such as environmental pollution, unsafe products, human health concerns, depletion of crop diversity, soil sickness and reduction of crop productivity. Several crops including alfalfa, buckwheat, maize, rice, rye, sorghum, sunflower, wheat, etc. are affected either by their own toxicity or phytotoxin exudates when their residues decompose in the soil, that show strong suppression on weed emergences. Allelopathic crops when used as cover crop, mulch, smother crops, green manures, or grown in rotational sequences are helpful in reducing noxious weeds and plant pathogen, improve soil quality and crop yield. Those crop plants, particularly the legumes, incorporated at 1–2 tons ha⁻¹ (alfalfa, buckwheat, rice by-products), which can give weed reduction and increase of rice yield by 70 and 20 %, respectively, are suggested for use as natural herbicides. Allelochemicals from allelopathic crops may aid in the development of biological herbicides and pesticides. Cultivating a system with allelopathic crops plays an important role in the establishment of sustainable agriculture. The introduction of allelopathic traits from accessions with strong allelopathic potential to the target crops will enhance the efficacy of crop allelopathy in future agricultural production.  相似文献   

Production of interspecific hybrids between Glycine max and G. tomentella through embryo culture     

Gyu Hwa Chung  Jin Hyeong Kim 《Euphytica》1990,48(2):97-101

Summary Interspecific hybrids have been obtained in an incompatible cross between Glycine max and G. tomentella through the in vitro culture of hybrid embryos. The percentage of successful pod setting in the crosses averaged 12.8% but there were marked differences depending on the soybean cultivar used as the female parent. Hybrid embryos at globular to heart stages were extracted from the embryo sac 15–25 days after pollination and cultured in vitro. Hybrid plants were successfully obtained by culturing the embryos on B5 medium supplemented with 0.1 mg/l IBA followed by culture on B5 medium supplemented with 0.1 mg/l TBA plus 0.25 mg/l 2-iP. The F1 plants resembled the wild male parent in growth form, but had an intermediate leaf shape between that of the parents.  相似文献   

Acidic Precipitation and Large-Scale Transport of Air Pollutants Observed in Korea     

Yong-Seung Chung  Hak-Sung Kim  Kie-Hyun Park 《Water, air, and soil pollution》2001,130(1-4):367-372

Acid rain at a rural site in Chongwon (KHN), central Korea has been under observation since 1990. To substantiate the 10-year observations, rain samples from six other sites were also collected and analyzed. Results show that acid rain occurred frequently at all sites. At KHN, seasonal and annual variability of pH values had a 10-year VWM value of 4.67 and an arithmetic mean value of 5.09. In general, a high value of pH occurred during springtime when the occurrence of yellow sand (dust) was prevalent in East Asia; during summer, heavy rain usually contained neutral pH values. During other seasons, the degree of acidic precipitation usually coincided with certain air flows. Most frequently, air flows and cyclones coming from south and central China often resulted in precipitation with low pH values observed in Korea. Using daily satellite observations from 1996, we have also been monitoring the long-range and large-scale transport of air pollutants (LSTAP) over East Asia. Many samples of LSTAP were obtained when they were crossing the Yellow Sea and moving over the Korean Peninsula and to SW Japan. The width of the Yellow Sea is ca. 350～700 km and air pollutants take 1～2 days to cross it. Cases of acid rain in Korea clearly correlate wth the LSTAP from source regions in China.  相似文献   

Volatile components in crabmeats of Charybdis feriatus   总被引：1，自引：0，他引：1  

Chung HY 《Journal of agricultural and food chemistry》1999,47(6):2280-2287

The volatile components of different meats (legs with claws, body, and carapace) of a popularly consumed edible crab in Asia, Charybdis feriatus, were investigated. Samples were extracted by simultaneous steam distillation-solvent extraction and analyzed by gas chromatography/mass spectrometry. Among 177 compounds detected, 130 were positively identified. Seventy-six compounds were previously reported in other crab species. A greater number of naphthalenes were detected in this crab compared with other crabs in the literature. Aromatic compounds, alcohols, and sulfur-containing compounds were the three predominant groups with >15 components. Carapace tissue contained a greater number of volatile components in each group, except for sulfur-containing compounds. Most of the common components in the leg meat and the body meat were found at similar levels (p > 0.05). Carapace tissue generally had the highest quantity of common components among the meats. The higher levels of volatile components present in the carapace tissue might account for its stronger flavor compared with the other meats. Furthermore, the higher number of aldehydes and lower number of sulfur-containing compounds detected in the carapace meat might contribute to its unique flavor.  相似文献   

Schisandrin B protects against solar irradiation-induced oxidative injury in BJ human fibroblasts     

Chiu PY  Lam PY  Yan CW  Ko KM 《Fitoterapia》2011,82(4):682-691

The effects of schisandrin B (Sch B) and its analogs on solar irradiation-induced oxidative injury were examined in BJ human fibroblasts. Sch B and schisandrin C (Sch C) increased cellular reduced glutathione (GSH) level and protected against solar irradiation-induced oxidative injury. The photoprotection was paralleled by decreases in the elastases-type protease activity and matrix-metalloproteinases-1 expression in solar-irradiated fibroblasts. The cytochrome P-450-mediated metabolism of Sch B or Sch C caused ROS production. The results suggest that by virtue of its pro-oxidant action and the subsequent glutathione antioxidant response, Sch B or Sch C may offer the prospect of preventing skin photo-aging.  相似文献   

Ochratoxin A in Korean food commodities: occurrence and safety evaluation     

Park JW  Chung SH  Kim YB 《Journal of agricultural and food chemistry》2005,53(11):4637-4642

To evaluate the exposure of Koreans to ochratoxin A, we conducted a survey in 2003 for ochratoxin A in various domestic food commodities: 60 polished rices, 22 barleys, 35 wheat flours, 46 beers, and 14 unstrained rice wine (makkolli) samples. They were analyzed for ochratoxin A using immunoaffinity column and high-performance liquid chromatography (HPLC)-fluorescence detection, and the positive samples were confirmed using HPLC-tandem mass spectrometry. By combining results from different surveys on the levels of ochratoxin A in selected foods and the consumption patterns, we obtained the Korean probable daily intakes (PDI) of ochratoxin A. The polished rice commodity had the highest mean levels of ochratoxin A, which ranged from 0.2 (not detected, i.e., ND = 0) to 1.0 ng/g (ND = limit of detection, i.e., LOD). The estimated PDI for all Koreans fell into the range of 0.8-4.1 ng/kg bw/day, while for heavy consumers the estimates ranged from 1.7 to 9.1 ng/kg bw/day, which did not exceed the PTDI value (14 ng/kg bw/day). Staple rice is the major contributor (>90%) to the Korean dietary intake of ochratoxin A. On the basis of these estimates, it may be concluded that there is at present no considerable risk of ochratoxin A exposure for the average Korean consumer.  相似文献   

Quantitative extraction of macro-invertebrates from temperate and tropical leaf litter and soil: efficiency and time-dependent taxonomic biases of the Winkler extraction   总被引：2，自引：0，他引：2  

Frank-Thorsten Krell  Arthur Y.C. Chung  Emma DeBoise  Paul Eggleton  Alessandro Giusti  Kelly Inward  Sylvia Krell-Westerwalbesloh 《Pedobiologia》2005,49(2):175-186

Winkler extractors, a simple device presumed to extract macro-invertebrates efficiently from soil and litter samples, is being used increasingly in ecological surveys and functional studies of soil macro-invertebrate communities. In this study the extraction efficiency and taxonomic bias of the Winkler extraction are evaluated for extraction periods of 3 h up to 7 weeks, calibrated by hand-sorting after 7 weeks. The method extracts most macro-invertebrates completely or to a proportion of over 90% except Isopoda, Diplopoda and Mollusca. However, for an exhaustive result, a long extraction period of several weeks is necessary. For the most speciose group (adult beetles) and for the commonly most abundant group (ants), a short extraction of 3 days was sufficient to get 70% of the individuals and nearly all species. Three days was also sufficient to recover the rank abundance order of beetle families, while for ‘higher taxa’ and for Chilopoda species, 4 and 3 weeks were necessary, respectively. Optimum extraction times for the abundant macro-invertebrate groups and possible adjustment factors for the soil macro-invertebrates of temperate woodlands are proposed to compensate the taxonomic bias caused by short extraction periods. However, for recording an accurate snapshot of the soil and litter fauna at a particular time, shorter extraction periods are advisable because of the short life cycle of many soil invertebrates causing emergence of later stages or a second generation during longer extraction periods. The problem of contamination of samples is also discussed.  相似文献