Platelet-Rich Plasma as an Adjunctive Therapy for the Management of a Severe Chronic Distal Limb Wound in a Foal     

Catalina López  Jorge U. Carmona  MVZ  MSc  PhD 《Journal of Equine Veterinary Science》2014

Distal limb wounds are frequent in horses after traumatic events. The use of platelet-rich plasma (PRP) represents a simple method of treating wounds in equines. A case of a chronic severely contaminated distal limb wound in an 8-month-old foal is presented. The patient was managed with the combination of standard wound therapy (debridement and bandages), surgery, and PRP obtained by a manual tube protocol. No complications were observed with the PRP treatment, and the foal was fully recovered 2 months later. The results from this case report indicate that PRP and its by-products (platelet-poor plasma) could be used as an adjunctive treatment in severe distal limb wounds in horses. A clinical controlled study should be performed to test this hypothesis.  相似文献   

Foaling Rates After Surgical Repair of Ventral Cervical Lacerations Using a Trendelenburg Position in 18 Anesthetized Mares     

John Mark O' Leary BVMS  Dwayne Rodgerson DVM  MSc  Diplomate ACVS  Michael Spirito DVM  Jorge Gomez MVZ  Diplomate ACVS 《Veterinary surgery : VS》2013,42(6):716-720

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In vitro Evaluation of the Torsional Strength Reduction of Neonate Calf Metatarsal Bones with Bicortical Defects Resulting from the Removal of External Fixation Implants     

Stefano Brianza DVM  PhD  Eng.  Susan Vogel DVM  Stephan Rothstock Eng.  Andrè Desrochers DVM  MS  Diplomate ACVS  Ludovic Boure DVM  MSc  DES  Diplomate ACVS & ECVS 《Veterinary surgery : VS》2013,42(1):75-78

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Nutritional Programming of Intrauterine Development: A Concept Applicable to the Horse?     

Abigail L. Fowden  Juanita K. JellymanOrlando A. Valenzuela  DVM  MSc  Alison J. Forhead  PhD 《Journal of Equine Veterinary Science》2013

Human epidemiological observations and studies of experimental animals have shown that low birth weight is associated with adult phenotypes characterized by abnormalities in cardiovascular, metabolic, and endocrine function. In human populations, these changes can lead to overt degenerative diseases such as hypertension and type 2 diabetes. Because fetal growth depends primarily on the nutrient supply, the associations between birth weight and adult phenotype have been linked to poor nutrition in utero. The fetal supply of nutrients and oxygen depends on both the availability of these substances in the mother and the functional capacity of the placenta to supply them to the fetus. This review takes a comparative approach to examining the nutritional programming of adult physiological phenotype in mammals with emphasis on the horse where possible. It considers the role of maternal nutritional state and placental function in these processes and discusses the cellular and molecular mechanisms operating in utero that are responsible for the epigenetic regulation of phenotypical diversity.  相似文献   

Effects of Pentoxifylline on Equine Epididymal Sperm     

Priscilla N. Guasti  Gabriel A. MonteiroRosiara R. Maziero  MSc  Ian MartinBruno R. Avanzi  MSc  José A. Dellaqua Jr.Frederico O. Papa  PhD 《Journal of Equine Veterinary Science》2013

This study evaluated whether pentoxifylline (PTX) present in the flushing extender influenced the function of equine epididymal spermatozoa after recovery and after thawing. For this experiment, 58 testicles from 29 Brazilian Jumping Horses were used. Cauda epididymides of each stallion were separated and flushed with a skim milk extender, with or without 7.18 mM PTX and then subjected to the freezing process. Samples flushed with the extender containing PTX showed a significant increase in total motility, progressive motility, straight line velocity, curvilinear velocity, and percentage of rapid sperm immediately after the recovery of epididymal sperm and after 15 minutes of incubation at 37°C (P < .05). However, the presence of PTX in the flushing extender did not affect the post-thaw motility parameters or plasma membrane integrity (P > .05). The results of this study showed that the PTX present in the flushing extender improved motility parameters of recently recovered epididymal sperm and had no deleterious effects on plasma membrane integrity and freezability of equine epididymal sperm.  相似文献   

Effects of oral caffeine administration to sows with induced parturition on hypoxia in piglets     

Paola Superchi  Claudio MazzoniPaola Zanardelli  Chiara PiancastelliErnesto Mario Zambini  Valentino BerettiAlberto Sabbioni 《Livestock Science》2013,157(1):372-377

To counteract the effects of perinatal hypoxia in piglets, the oral administration of caffeine to sows with induced parturition was evaluated. On day 113 of gestation 9 sows received 27 mg/kg body weight (BW) of caffeine mixed with 200 g of standard diet. The same amount of feed without the addition of caffeine was administered to 9 control sows. Additionally, on day 113 of gestation, all sows were treated by two injections in the perianal area of 1 mg of alfaprostol (at 8:00 am and 14:00 pm), and on the morning of farrowing by 14 IU of oxytocin in the perianal area. Caffeine did not affect BW of piglets and size of litters; however piglets from treated sows showed a higher capacity to adapt to extra-uterine life. Particularly, they showed a greater thermoregulatory ability (P<0.001) and a higher percentage of viability score≥7 compared to piglets from control sows (92% vs. 75%, respectively; P=0.030). Caffeine furthermore reduced the frequency of high serum biopterin values (>80 nmol/L) in piglets born from treated sows (P=0.001). In conclusion, the caffeine orally administered to sows with induced parturition showed a protective effect on the consequences of neonatal hypoxia in tissue ischemia–reperfusion injury in piglets.  相似文献   

Iron oxide‐humic acid coprecipitates as iron source for cucumber plants     

Erika Di Iorio  Claudio Colombo  Ruggero Angelico  Roberto Terzano  Carlo Porfido  Fabio Valentinuzzi  Youry Pii  Tanja Mimmo  Stefano Cesco 《植物养料与土壤学杂志》2019,182(6):921-933

In soil, iron (Fe) solubility depends on complex interactions between Fe minerals and organic matter, but very little is known about plant availability of Fe present in Fe oxides associated with humic substances. For this purpose, this study investigates the effect of Fe mineral crystallinity in the presence of humic acids (HA) on Fe availability to plants. Four Fe–HA mineral coprecipitates were prepared, either in the presence or absence of oxygen, i.e., two goethite (G)‐HA samples containing large amounts of Fe as nanocrystalline goethite and ferrihydrite mixed phases, and two magnetite (M)‐HA samples containing crystalline magnetite. Bioavailability studies were conducted in hydroponic systems on cucumber plants (Cucumis sativus L.) grown under Fe deficient conditions and supplied with the Fe–HA coprecipitates containing goethite or magnetite. Results showed that plants grown in the presence of Fe–HA coprecipitates exhibited a complete recovery from Fe deficiency, albeit less efficiently than plants resupplied with Fe‐chelate fertilizer used as control (Fe‐diethylene triamine penta acetic acid, Fe‐DTPA). However, the supply with either G‐ or M–HA coprecipitates produced different effects on plants: G–HA‐treated plants showed a higher Fe content in leaves, while M–HA‐treated plants displayed a higher leaf biomass and SPAD (Soil–Plant Analysis Development) index recovery, as compared to Fe‐DTPA. The distribution of macronutrients in the leaves, as imaged by micro X‐ray fluorescence (µXRF) spectroscopy, was different in G–HA and M–HA‐treated plants. In particular, plants supplied with the poorly crystalline G–HA coprecipitate with a lower Fe/HA ratio showed features more similar to those of fully recovered plants (supplied with Fe‐DTPA). These results highlight the importance of mineral crystallinity of Fe–HA coprecipitates on Fe bioavailability and Fe uptake in hydroponic experiments. In addition, the present data demonstrate that cucumber plants can efficiently mobilize Fe, even from goethite and ferrihydrite mixed phases and magnetite, which are usually considered unavailable for plant nutrition.  相似文献   

Citrantifidiene and citrantifidiol: bioactive metabolites produced by Trichoderma citrinoviride with potential antifeedant activity toward aphids     

Evidente A  Ricciardiello G  Andolfi A  Sabatini MA  Ganassi S  Altomare C  Favilla M  Melck D 《Journal of agricultural and food chemistry》2008,56(10):3569-3573

Two novel metabolites with potential antifeedant activity were isolated from cultures of the fungus Trichoderma citrinoviride strain ITEM 4484 grown in solid-state fermentation on sterile rice kernels. The producing strain was identified at species level by sequence analysis of the internal transcribed spacer regions ITS-1 and ITS-2 of the nuclear rDNA and a fragment of the translation elongation factor gene TEF-1alpha. Fractionation by column chromatography and TLC of the culture organic extract, followed by feeding preference tests on the aphid pest Schizaphis graminum (Rondani), allowed the purification of 5.8 and 8.9 mg/kg of culture of two bioactive metabolites, which were named citrantifidiene and citrantifidiol ( 1 and 2). Citrantifidiene and citrantifidiol, whose structures were determined by spectroscopic methods (NMR and MS) are a symmetrical disubstituted hexa-1,3-dienyl ester of acetic acid and a tetrasubstituted cyclohexane-1,3-diol, respectively. The pure metabolites influenced the feeding preference of S. graminum restraining individuals from feeding on wheat leaves dipped in 5% aqueous methanol solution containing 0.57 mg/mL of citrantifidiene or 0.91 mg/mL of citrantifidiol.  相似文献   

Transgenic rice lines that include barley genes have increased tolerance to low iron availability in a calcareous paddy soil   总被引：2，自引：0，他引：2  

Motofumi SUZUKI  Kendi Claudio MORIKAWA  Hiromi NAKANISHI  Michiko TAKAHASHI  Masahiko SAIGUSA  Satoshi MORI  Naoko K. NISHIZAWA 《Soil Science and Plant Nutrition》2008,54(1):77-85

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Importance of contact between rice roots and co-situs applied fertilizer granules on iron absorption by paddy rice in a calcareous paddy soil     

Claudio Kendi MORIKAWA  Masahiko SAIGUSA  Naoko Kishi NISHIZAWA  Satoshi MORI 《Soil Science and Plant Nutrition》2008,54(3):467-472

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