Antitrypanosomal activity of polycarpol from Piptostigma preussi (Annonaceae)     

Igor Ngantchou  Ernestine Nkwengoua  Yves Nganso  Barthelemy Nyasse  Colette Denier  Veronique Hannaert  Bernd Schneider 《Fitoterapia》2009

Polycarpol, sitosterol and sitosterol-3-O-β-D-glucoside isolated for the first time from Piptostigma preussi (Annonaceae) occur regularly in some Annonaceae such as Piptostigma genus. Polycarpol exhibits interesting antitrypanosomal activity with an ED₅₀ value of 5.11 µM on Trypanosoma brucei cells. Moreover, it inhibits T. brucei glycolytic enzymes GAPDH and PFK with IC₅₀ values of 650 and 180 µM respectively.  相似文献   

Simazine dealkylation in conjunction with citrus roots     

Lowell S. Jordan  Virgil A. Jolliffe 《Pest management science》1973,4(4):467-472

Simazine (2-chloro-4,6-bis(ethylamino)-s-triazine) was metabolised by dealkylation in the presence of citrus tree roots growing in nutrient solution. Dehalogenation to hydroxysimazine was not observed. Removal of citrus roots from the solution caused dealkylation to cease. Dealkylation did not occur in the nutrient solution in the absence of roots.  相似文献   

Evaluation of the discriminatory power of variable number tandem repeat (VNTR) typing of Mycobacterium bovis strains     

Hilty M  Diguimbaye C  Schelling E  Baggi F  Tanner M  Zinsstag J 《Veterinary microbiology》2005,109(3-4):217-222

The discriminatory power of variable number tandem repeat (VNTR) typing based on 16 known loci (12 MIRUs, 3 ETRs and VNTR 3232) was assessed for Mycobacterium bovis strains collected sequentially at the slaughterhouse of N'Djaména, Chad. Of 67 M. bovis strains analyzed, 67% were clustered. In this study, VNTR typing was highly discriminative with an overall allelic diversity (h(oa)) of 0.922. We defined five loci (ETR A, B, C and MIRU 26, 27) as highly (h>0.25), two loci (MIRU 4, and VNTR 3232) as moderately (0.11相似文献   

Malignant hyperthermia in a horse anesthetized with halothane     

Aleman M  Brosnan RJ  Williams DC  LeCouteur RA  Imai A  Tharp BR  Steffey EP 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2005,19(3):363-367

Ahealthy, 565-kg, 14-year-old Quarter Horse gelding, negative for equine protozoal myelitis on serum Western blot and for hyperkalemic periodic paralysis (HYPP) by polymerase chain reaction, was selected for use in an electrophysiology study performed under inhalation anesthesia. The study was performed by following the guidelines of an Animal Use and Care Committee Protocol of the University of California at Davis (UCD 8578). Anesthesia was induced with halothane delivered via a face-mask. The horse was intubated 17 minutes later with an orotracheal tube and anesthesia was maintained with halo-thane by using a semiclosed, large-animal breathing circuit. Although muscle relaxation was otherwise good, the horse's ears remained tensed in a caudal direction throughout the study  相似文献   

Spiroplasma citri Spiralin Acts In Vitro as a Lectin Binding to Glycoproteins from Its Insect Vector Circulifer haematoceps     

Killiny N  Castroviejo M  Saillard C 《Phytopathology》2005,95(5):541-548

ABSTRACT In order to understand the molecular mechanisms underlying transmission of Spiroplasma citri by the leafhopper Circulifer haematoceps, we screened leafhopper proteins as putative S. citri-binding molecules using a spiroplasma overlay assay of protein blots (Far-western assay). Insect proteins were separated by one- or two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis, blotted, and probed with S. citri proteins. In this in vitro assay, we found that spiroplasma proteins exhibited affinity for seven leafhopper proteins. The interactions between S. citri proteins and insect proteins with molecular masses of 50 and 60 kDa were found to be sugar sensitive. These insect proteins were identified as high mannose N-glycoproteins, which support an interaction of glycoprotein-lectin type with S. citri proteins. Lectin detection in S. citri has revealed only one protein of 24 kDa. Using a leafhopper protein overlay assay on an S. citri protein blot, one spiroplasma protein with a similar molecular mass of 24 kDa was shown to display an insect protein-binding capacity. This protein was identified as the spiralin, which is the most abundant membrane protein of S. citri. Far-western experiments performed with purified spiralin and insect glycoproteins confirmed the binding of spiralin to the insect glycoproteins of 50 and 60 kDa. Thus, the spiralin could play a key role in the transmission of S. citri by mediating spiroplasma adherence to epithelial cells of insect vector gut or salivary gland.  相似文献   

Evaluation of a broad range real-time polymerase chain reaction (RT-PCR) assay for the diagnosis of septic synovitis in horses     

Colette R. Elmas  Judith B. Koenig  Dorothee Bienzle  Nicola C. Cribb  Natalia Cernicchiaro  Nathalie M. Coté  J. Scott Weese 《Canadian journal of veterinary research》2013,77(3):211-217

Septic synovitis is a potentially debilitating and life-threatening disorder in horses. We hypothesized that a universal bacterial real-time PCR (RT-PCR) assay would have improved sensitivity and decreased turn-around time for detection of bacteria in synovial fluid (SF) samples. Forty-eight SF samples were collected from 36 horses that presented to two referral institutions with suspected septic synovitis. Universal RT-PCR, bacterial culture and SF analysis were performed on all samples, and an interpretation on the sample being septic or not was derived by three board certified specialists from the history, clinical assessment and SF characteristics. RT-PCR results were compared to a composite standard comprised of positive culture and interpretation by all three specialists of samples as “septic”. For 41 of 48 samples (85%), culture and RT-PCR results were concordant. Compared to the composite standard, 83% of samples were correctly classified by RT-PCR (turn-around time of approximately 4 hours). Relative sensitivity and specificity of RT-PCR were 87% and 72% respectively, and 56% and 86% for culture. Hence, universal RT-PCR was a rapid and highly sensitive test, which may accelerate diagnosis and improve outcome for horses with septic synovitis.  相似文献   

Effects of temporal floral resource availability and non-crop habitats on broad bean pollination     

Eckerter  Philipp W.  Albrecht  Matthias  Bertrand  Colette  Gobet  Erika  Herzog  Felix  Pfister  Sonja C.  Tinner  Willy  Entling  Martin H. 《Landscape Ecology》2022,37(6):1573-1586

Landscape Ecology - Flowering plants can enhance wild insect populations and their pollination services to crops in agricultural landscapes, especially when they flower before the focal crop....  相似文献   

Selective one-step extraction of Arabidopsis thaliana seed oleosins using organic solvents     

D'Andréa S  Jolivet P  Boulard C  Larré C  Froissard M  Chardot T 《Journal of agricultural and food chemistry》2007,55(24):10008-10015

Oleosins are hydrophobic proteins from oleaginous seeds, surrounding and stabilizing oil bodies. They are known to display interesting interfacial properties. Specific sera were raised against four different A. thaliana oleosins and used in dot-blot assays for oleosin quantification. These assays were used to set up extraction of oleosins from A. thaliana seeds. One mixture of chloroform/methanol gave optimal oleosin extraction. Extracted proteins represented 9% of seed proteins and were identified by immunoblot and proteomic analyses. Oleosins accounted for 79% of the extracted proteins. This simple one-step procedure allows selective extraction and concentration of oleosins from seeds without tedious oil body purification. Oleosin extract was indeed used to demonstrate the presence of the rare oleosin S5 in mature seeds. Moreover, this method will be useful to investigate the potential use of oleosins as emulsifier and to question their possible allergenicity.  相似文献