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A temperature sensitive (ts) vaccine strain designated ts-11 was selected after exposure of a low passage culture of the immunogenic Australian field isolate (strain 80083) of Mycoplasma gallisepticum to 100 mg/ml of N-methyl-N-nitro-N-nitrosoguanidine. Viable counts (assayed as colour changing units (CCU)/25 microliters) of a thawed stock culture of ts-11 were typically log10 3 to log10 5 higher when incubated at 33 degrees C (the permissive temperature) than duplicate viable counts incubated at 39.5 degrees C (the restrictive temperature). Doses of approximately 2 x 10(7) CCU of ts-11 caused no gross lesions or loss of egg production when inoculated into the air sacs of susceptible chickens and no clinical or pathological signs of sinusitis when inoculated into the infraorbital sinuses of susceptible turkey poults, whereas the parent strain 80083 was demonstrably pathogenic. However, 1 of 10 poults inoculated intra-abdominally with approximately 2 x 10(7) CCU of ts-11 did show signs of mild airsacculitis. Eight-week-old pullets were vaccinated by eye drop with up to 1.4 x 10(7) CCU of ts-11 and simultaneously subjected to several stressful management practices, without apparent ill effects. Administration by coarse aerosol of 5 ml of ts-11 vaccine/25 day-old broilers, with or without 25 doses of infectious bronchitis virus vaccine caused no obvious signs of respiratory disease. The non virulent ts phenotype was maintained after 3 passages of strain ts-11 in chickens. Chickens vaccinated 3 weeks previously with ts-11 or with strain 80083 were placed in contact with susceptible chickens for a period of 2 weeks.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
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白背毛木耳菌株栽培对比试验 总被引:1,自引:0,他引:1
对12个白背毛木耳菌株的农艺性状进行栽培对比。结果表明:经初步筛选,‘06283’、‘43012’和‘AP201’等3个优良菌株可以在生产上推广;菌株‘AP201’产量最高为74.64g/袋,与对照相比增产15.7%,干物质含量也高,但其菌种活力较弱,不耐高温,生长速度慢,而且该菌株耳片腹面棱脊多,呈网状,不符合目前的出口外销要求,不建议生产上大规模应用;菌株‘06283’和‘43012’的产量均较高,分别为69.90、64.69g/袋,与对照相比分别增产8.4%、0.3%,且耳片大而厚,抗污染能力强,‘43102’的产量虽然比‘06283’及‘AP201’稍低,但其干物质含量比值最大,这2个菌株的产品适合于出口,市场价格较好,符合生产品种要求。 相似文献
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Eperythrozoon wenyonii infection in dairy cattle 总被引:1,自引:0,他引:1
J A Smith M A Thrall J L Smith M D Salman S V Ching J K Collins 《Journal of the American Veterinary Medical Association》1990,196(8):1244-1250
Approximately 10 of 100 young heifers that had recently delivered their first calf--members of a large Colorado dairy herd--had a syndrome of swollen teats and distal portions of the hind limbs, prefemoral lymphadenopathy, transient fever, rough coat, decreased milk production, and subsequent weight loss and reproductive inefficiency. Acute clinical signs of disease were associated with large numbers of Eperythrozoon wenyonii seen on blood smears, and resolution of signs correlated with reduction or disappearance of the parasite. Other known causes of peripheral edema could not be documented. The parasite was transmitted to 4 of 7 nonlactating dairy cows destined to be culled and a splenectomized calf via IV inoculation of blood from parasitemic heifers, but clinical signs of infection were not induced. 相似文献
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Twelve goats were inoculated with 40,000 third-stage Haemonchus contortus larvae and two were killed on each of Days 4, 7, 11, 14, 18 and 21 after inoculation (DAI). The number of worms that established, and the site of development were recorded. More worms established in the fundic, than in the middle or pyloric thirds of the abomasum. Early development occurred within the mucosa; emergence into the lumen started between 7 and 11 days after infection. By 4 DAI, all worms had completed the third moult to the L4 stage. At 11 DAI the majority of the worms were adults. A mean of 13.2% of the female worms had eggs in their uteri at 18 DAI; by 21 DAI more than half of the female worms had eggs in their uteri. The development of H. contortus was essentially similar to that described in sheep. 相似文献
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An angiosarcoma involving the right frontal and maxillary sinuses in a nine-year-old Thoroughbred mare was studied post mortem by light and electron microscopy. Important diagnostic features included malignant endothelial cells lining ill-defined vascular spaces, supported by other vasoformative elements. Rhabdomyoblasts were also found. The tumour is apparently rarely seen in the nasal passages of the horse. 相似文献
59.
The relationship between air quality-its dust content-and respiratory diseases of animals in the food industry and otherwise in examined. The correlation between dust content and incidence of disease in farm animals is also discussed. Lesions caused either directly or indirectly by dusttransported microorganisms are reviewd. Effects of dust on animal performance are included.This review is an attempt to compile available information on the natural occurrence of stable dust and the possibilities for its measurement and control. 相似文献
60.
两栖蔊菜(Rorippa amphibia)是近年来备受关注的入侵植物,主要出现在草坪中,并有不断向各种生境扩张的趋势,往往会成为其入侵群落中的优势种,因此研究其种群和群落特征及环境影响因素,对于更好地了解其入侵机制具有重要理论意义。本研究调查了不同小生境中受到两栖蔊菜入侵的草坪群落,采用扩散系数(C)、负二项参数(K)、平均拥挤度(m*)、丛生指标(I)、聚块性指标(PI)、Cassie指标(CA)和Green指数(GI) 7个指标对两栖蔊菜的空间分布格局进行研究,并分析两栖蔊菜的种群密度、盖度等特征。运用聚类分析、主成分分析(PCA)探讨了群落类型的差异及主要环境影响因子。结果表明,在不同生境条件下两栖蔊菜种群均呈聚集分布。无遮阴和半遮阴条件下,两栖蔊菜的种群密度分别为全遮阴条件的2.93倍和2.73倍,盖度分别为4.52倍和4.79倍,高度分别为1.57倍和2.37倍。即在全遮阴条件下两栖蔊菜种群的密度、高度、盖度均最低,且与无遮阴生境种群间差异显著(P <0.05);半遮阴条件下,两栖蔊菜的高度和盖度最高,与全遮阴生境下差异显著(P <0.05),与无遮阴生境种群高... 相似文献